The scaling law was examined using numerical simulation for fire phenomena in a compartment with a horizontal natural opening in this study. The results of the large-scale conversion of the scaled model numerical simulation results ([Small-scale] condition) from the earlier studies obtained by applying the scaling law were compared to those of the large-scale numerical simulation performed in this study ([Large-scale] condition). The overall trends for the effects of horizontal natural opening area and fire source location on the discharge mass flow rate through the horizontal natural opening, temperature distribution in the compartment, and flow velocity through the horizontal opening were found to be identical under the [Small-scale] and [Large-scale] conditions. A complex bidirectional flow was observed through the horizontal natural opening under the conditions of this study and the accuracy in predicting the average W-velocity value based on the scaling law was low; however, the accuracy in predicting the range of velocity fluctuation was higher than the average value under certain conditions. Based on the standard deviation of the numerical simulation results, the percentages of the data within ± 30% of the difference between the [Small-scale] and [Large-scale] conditions relative to the total number of data were 100, 75, and 88% for the discharge mass flow rate, temperature, and flow velocity through the horizontal natural opening, respectively. The accuracy of the scaling law was found to be high for the discharge mass flow rate under the conditions considered in this study.