In this study, an air-mediated local heating method is developed for Lab-on-a-Disk platforms. The hot air is blown on a rotating disk through a dual-sided nozzle for heating up the specific ring-shaped regions on the rotating disk surface. The local heating can be used for combining different thermal based fluidic functions and reactions in a single disk. A numerical simulation has been performed for analyzing the air flow and temperature distribution on different regions of the disk and surrounding air. The effects of the operational parameters including hot air temperature and flow rates as well as the angular velocity of the disk are evaluated using an exclusive experimental setup. The results show that without considerable thermal effect on other regions of the disk, a uniform temperature can be achieved for the liquid sample (25 μl in this study) with a considerable temperature changing rates. The proposed method has been implemented for a PCR amplification of the β actin gene. The heating and cooling rates of the thermocycling process were 1.8 °C/s and 2.2 °C/s respectively.