Currently, with the intensification of global education competition, how to cultivate innovative and versatile talents has become the focus of education today. Informal learning spaces (ILS) have received widespread attention due to their benefits for interdisciplinary interaction. Many forms of ILS have been built, and coffee shops are one of them. However, there is no uniform standard for the design of cafes as ILS, and there are many problems with the spatial quality. This study took a university cafe as a case study and conducted research on the environmental quality and influencing factors from the perspective of environmental behavior. In terms of research methods, this study adopted a combination of ultra-wideband positioning, questionnaire surveys, and environmental data measurements. This research method could obtain the complete relationship between user behavior, time, and location, which improved the shortcomings of traditional research methods of fragmented and accidental data acquisition, and helped to obtain more accurate research results. The study identified the impact proportions of four criteria layer elements and 26 scheme layer factors, which was also the main contribution of this study. In terms of four criteria layer elements, the physical elements had the highest proportion, accounting for 32.46%. The ontology elements ranked second, accounting for 27.07%. The atmosphere elements ranked third, accounting for 24.19%. Finally, facility elements accounted for 16.29%. The top three scheme layer factors were equipment and facilities, noise, and illumination, with weights of 11.18%, 10.71%, and 8.35%, respectively. The study summarized the development directions of cafes as ILS, such as considering the learning needs of different groups, installing sound-absorbing and soundproof devices, adding grilles to external windows, and improving glare problems.