The Helianthus tuberosus , it is one of the important Tuberous plants . at present time Where this plant was used in the study , Precisely the juice fruit of Helianthus tuberosus , as were used widely in promoting immune system , stimulate blood circulation, controlling cholesterol , blood pressure, diabetes, muscle , preservation of food , Antibacterial , the Beautify skin and Biofuels. This study conducted on juice fruit Helianthus tuberous . it taken from different areas in Baghdad / Iraq . after Buy them from the local market , washed, dried and squeeze . this study aimed to identify the chemical compounds and trace elements in the Helianthus tuberosus juice using tests chemical differents , usage a measurement technique (GC-MS) ,and the FTIR spectrum. and their impact on the liver function of domestic birds and species of microorganisms , The study is giving that the juice of the fruit Helianthus Tuberosus is Acidic nature and near from the value acidic for water , The study showed that the extract contains a group of carbohydrate compounds, phenol compounds, tannins , resins, flavonoids, alkaloids, turbines, unsaturated fatty acids, polymers. and the Inulin compound. The Study showed the ability of juice Helianthus Tuberosus in concentration of 15%, increasing stimulate of SGPT levels and SGOT in serum (p <0.01), while the levels of ALP, LDL, TG were decreased (p <0.05), The study shows extracting of phenolic compounds in fruit juice Helianthus Tuberousus when TLC and HPLC used a mixture (water, chloroform , acetic acid) and by (3: 6: 1) . This mixture had effect on multi pigment (gram pigments) . The effect was more in Gram positive bacteria (Escherichia coli Pseudomonas aeruginosa, Salmonella typhimurium), but its effect was less in Gram negative bacteria (Staphylococcus aureus, Staphylococcus epidermidis, Bacillus subtilis). lastly the study giving , through the quantitative estimate of the chemical elements of the juice of the Helianthus Tuberosus fruit , contained some important minerals of different concentrations with a capacity of (ppm).the presence of these elements led to increased enzymatic effectiveness through increased support for building bone tissue and the process of activating enzymes transfer of oxygen in the blood and increase the flow of all metabolic processes in the body and that these jobs will give an over view of the work and effectiveness of the nature activity of some organs of our body.