Local Landraces Research Articles

Greek Landrace Flours Characteristics and Quality of Dough and Bread.

Besides organic growing, ancient wheats and landraces are attracting the attention of scientists who are reassessing the healthy and dietary properties attributed to them by popular tradition. A total of eleven wheat flours and whole meal samples were analyzed, of which, nine originated from the organic farming of five Greek landraces (one einkorn, one emmer, two durum, and one soft wheat) and a commercial organically grown emmer cultivar. Two commercial conventional flours of 70% and 100% extraction rate were examined for comparison purposes. Chemical composition, micronutrients, phenolic profile, and quantification, and antioxidant activity of all samples were determined. Moreover, dough rheology and breadmaking quality were studied; Flours from local landraces were higher in micronutrients, phenolic content, and antioxidant activity than the commercial samples. The 90% extraction flour of the landrace, besides the highest protein content (16.62%), exhibited the highest content of phenolic acids (19.14 μg/g of flour), whereas the commercial refined emmer flour was the lowest (5.92 μg/g of flour). The same milling of the einkorn landrace also showed a higher specific volume (1.9 mL/g vs. 1.7 mL/g) and lower bread crumb firmness than the whole meal commercial emmer sample (33.0 N vs. 44.9 N). The results of this study showed that the examined Greek wheat landraces could be considered as a possible source of microelements, phenolics, and antioxidants with a beneficial effect in human health, and by using an appropriate breadmaking procedure, they could produce high-quality breads.

Determinants of Adoption of Improved Varieties of Wheat (Triticum aestivum), Teff (Eragrostis teff), and Maize (Zea mays L.) in Central Ethiopia

This study determined factors of the adoption of improved varieties of certified maize, teff, and wheat seeds in central Ethiopia. The logit model was applied to estimate the likelihood of adoption decisions. The result showed that 29% of household respondents adopted improved seeds, while 71% relied on their local landraces. The findings also revealed that farmers' decisions to adopt wheat, teff, and maize varieties were significantly influenced by educational level, farm size, farming experience, income, credit access, extension contact, farm input, and distance to the market. Thus, the study recommends that the government should focus on strengthening extension services, improving access to improved seeds, expanding timely agricultural inputs supply, improving market opportunities, equipping knowledgeable farmers who increase the use of new varieties, and making the land more economical by sharing agronomic practices are areas that need policy attention enhancing the adoption of certified seeds of improved varieties.

Pay more for me, I’m from the mountains! The role of the EU Mountain Product term and other credence attributes in consumers’ valuation of lamb meat

On-farm product differentiation represents a valuable opportunity for mountain agriculture. Consumers appreciate mountain products’ characteristics, but when signalling instruments are lacking, consumers might not be able to recognize (and pay for) them. Through a choice experiment, this paper considers consumers’ willingness to pay for three attributes (mountain origin through the EU Mountain Product term; local endangered landrace; agroecosystems stewardship through rotational grazing). It considers lamb meat production and North-Eastern Italian consumers, also exploring attitudes towards food and differences across groups of consumers. The highest willingness to pay is associated with the EU Mountain Product term, but communication strategies and public information provision are needed to enhance consumers’ overall knowledge.

Genetic diversity and structure of Slovenian native germplasm of plum species (P. domestica L., P. cerasifera Ehrh. and P. spinosa L.).

Slovenia has particular climatic, soil, geographic and historical conditions that lead to long tradition of plum cultivation and use. In this work, a set of 11 SSR and three universal cpDNA markers, as well as flow cytometry, were used to (1) evaluate the genetic diversity of 124 accessions of the three Prunus species (P. domestica L., P. cerasifera Ehrh., and P. spinosa L.), (2) investigate the possible involvement of P. cerasifera and P. spinosa species in P. domestica origin, (3) study the genetic relationships and variability among the most typical P. domestica accessions present in Slovenia. Ten haplotypes of cpDNA were identified and clustered into three groups according to the Neighbor-Joining analysis (NJ). All 11 SSR primer pairs were polymorphic, revealing 116 unique genotypes. A total of 328 alleles were detected with an average value of 29.82 alleles per locus, showing relatively high diversity. Bayesian analysis of genetic structure was used to identify two ancestral populations in the analyses of all three species as well as in a separate set consisting of P. domestica material only. Principal Coordinate Analysis (PCoA) showed that accessions clustered largely in agreement with Bayesian analysis. Neighbor-Joining analysis grouped 71 P. domestica accessions into three clusters with many subgroups that exhibited complex arrangement. Most accessions clustered in agreement with traditional pomological groups, such as common prunes, mirabelle plums and greengages. In this study, the analyses revealed within P. domestica pool valuable local landraces, such as traditional prunes or bluish plums, which seem to be highly interesting from a genetic point of view. Moreover, complementary approaches allowed us to distinguish between the three species and to gain insights into the origin of plum. The results will be instrumental in understanding the diversity of Slovenian plum germplasm, improving the conservation process, recovering local genotypes and enriching existing collections of plant genetic resources.

Agronomic, Physiological, Genetic and Phytochemical Characteristics of Onion Varieties Influenced by Daylength Requirements

The evaluation of A. cepa biodiversity provides onion breeders with great prospects to obtain plants with high resistance to environmental factors, remarkable yield, and product quality. Genetic diversity assessment of a collection of nine short- and long-day onion landraces and varieties originated from different Iranian regions, using ISSR markers and UPGMA dendrograms, was carried out for the first time. Short-day landraces (Sarze, Sarkoreh, and Kerman) originated from low latitude and short-day hybrids (Mirela and Soberana) demonstrated high genetic similarity, in terms of physiological responses to day length and temperature during the crop cycle and bulbing stage initiation. Contrarily, high latitude landraces (Azarshahr, White Gorgan, Kurdistan, and Esfahan) showed low biometrical, agronomic and genetic similarity with commercial short-day onions. Specific differences in morphological reactions between these two groups displayed diverse responses of plants to day length and temperature. Long- and short-day cultivars displayed significant differences in the dynamics of leaf and scale number, leaf and root length, dry and fresh weight, and bulbing ratio and bulb diameter, which were in accordance with ISSR data. The local landraces Azarshahr, Kurdistan, and Esfahan had high antioxidant status, suggesting high prospects for their utilization as an alternative to foreign F1 hybrid varieties.

Genetic diversity between local landraces and current breeding lines of pepper in China

Based on 22 qualitative traits, 13 quantitative traits, and 27 molecular markers (26 SSR and 1 InDel), in the current study we compared the diversity and population structure of 94 local landraces and 85 current breeding lines of pepper in China. The results showed that the Shannon Diversity indices of 9 qualitative traits and 8 quantitative traits in current breeding lines were greater than those of landraces, of which 11 were fruit organ-related traits. Compared with current breeding lines, the mean values of Gene Diversity index and Polymorphism Information content of local landraces were higher by 0.08 and 0.09, respectively. Population structure and phylogenetic tree analysis showed that the 179 germplasm resources could be divided into two taxa, dominated by local landraces and current breeding lines, respectively. The above results indicated that the diversity of quantitative traits of current breeding lines were higher than that of local landraces, especially traits related to fruit organs, but the genetic diversity based on molecular markers was lower than that of local landraces. Therefore, in the future breeding process, we should not only focus on the selection of target traits, but also strengthen the background selection based on molecular markers. Moreover, the genetic information of other domesticated species and wild species will be transferred to the breeding lines through interspecific crosses to expand the genetic background of the breeding material.

English

High-yielding and disease-resistant hybrid plant cultivars recently developed by breeders need evaluation for end-use. This study evaluated the influence of processing methods on the sensory acceptability of products (plantain chips, fried ripe plantains called “dodo” in Nigeria, boiled unripe and ripe plantains, and "amala," a dough, as it is called in Nigeria when unripe and ripe plantain flour is reconstituted in hot water) from selected hybrid plantain cultivars. Pita 26, Pita 27, Mbi egome, and Agbagba landrace cultivars were studied. Plantain pulps were subjected to frying (170°C for 2 min), boiling (100°C for 15 min), and drying (65°C for 48 h) at unripe and ripe stages before analyses. These food items were subjected to sensory evaluation. The panel of 20 people evaluated samples for texture, taste, flavor, appearance, color, stretchability, moldability, mouthfeel, and overall preference on a 9-point hedonic scale. The sensory studies showed statistically significant differences (P<0.05) between products and processing methods significantly affected the cultivars' sensory parameters and essential minerals and vitamins. Products from the Mbi egome cultivar were adjudged acceptable in terms of overall quality, followed by the Agbagba local landrace cultivar. The data in this study have shown that hybrid plantains have the potential to be used industrially.   Key words: Consumers acceptability, value added products, hybrid plantain, organoleptic. 

Indigenous Sudanese sorghum-based food: Secondary metabolites and antioxidant activities of traditional Sudanese nonalcoholic beverage Hulu-mur from two sorghum landraces.

Hulu-mur is a Sudanese traditional nonalcoholic beverage that is made from sorghum flour. This work determined the secondary metabolites and antioxidant activities of traditional Sudanese nonalcoholic beverage Hulu-mur from two local sorghum landraces Abjaro and Hegarii. The changes on the total phenolic content (TPC), total flavonoid content (TFC), carotene content, tannins, and antioxidant activity (DPPH, reducing power, and FRAP) were estimated during the preparation of the Hulu-mur flasks. For both landraces, a significant (p< .05) effect on the phytochemical compound and the antioxidant activity was observed during malting and fermentation of sorghum flour. However, the most increase in the TPC and carotene content was observed, whereas tannin and TFC were decreased in the Hulu-mur flasks compared with the malted and fermented samples. The antioxidant activity DPPH, TRP, and FRAP was significantly (p< .05) higher in Hulu-mur flasks than those of raw and processed flour. The partial least squares regression test stated a positive validation score of the Hulu-mur flasks prepared from the both landraces. In conclusion, Hulu-mur drink from Abjaro and Hegarii landraces contain high antioxidants compound, which could improve the health-promoting metabolites in Sorghum-based food.

Analysis of local cassava landraces and improved genotypes in response to infections by cassava mosaic begomoviruses under field conditions in Kenya

Analysis of local cassava landraces and improved genotypes in response to infections by cassava mosaic begomoviruses under field conditions in Kenya

Genetic Diversity and Population Structure of Common Bean (Phaseolus vulgaris L.) Landraces in the Lazio Region of Italy.

Common bean cultivation has historically been a typical component of rural economies in Italy, particularly in mountainous and hilly zones along the Apennine ridge of the central and southern regions, where the production is focused on local landraces cultivated by small-scale farmers using low-input production systems. Such landraces are at risk of genetic erosion because of the recent socioeconomic changes in rural communities. One hundred fourteen accessions belonging to 66 landraces still being grown in the Lazio region were characterized using a multidisciplinary approach. This approach included morphological (seed traits), biochemical (phaseolin and phytohemagglutinin patterns), and molecular (microsatellite loci) analyses to investigate their genetic variation, structure, and distinctiveness, which will be essential for the implementation of adequate ex situ and in situ conservation strategies. Another objective of this study was to determine the original gene pool (Andean and Mesoamerican) of the investigated landraces and to evaluate the cross-hybridization events between the two ancestral gene pools in the P. vulgaris germplasm in the Lazio region. Molecular analyses on 456 samples (four for each of the 114 accessions) revealed that the P. vulgaris germplasm in the Lazio region exhibited a high level of genetic diversity (He = 0.622) and that the Mesoamerican and Andean gene pools were clearly differentiated, with the Andean gene pool prevailing (77%) and 12% of landraces representing putative hybrids between the two gene pools. A model-based cluster analysis based on the molecular markers highlighted three main groups in agreement with the phaseolin patterns and growth habit of landraces. The combined utilisation of morphological, biochemical, and molecular data allowed for the differentiation of all landraces and the resolution of certain instances of homonymy and synonymy. Furthermore, although a high level of homozygosity was found across all landraces, 32 of the 66 examined (49%) exhibited genetic variability, indicating that the analysis based on a single or few plants per landrace, as usually carried out, may provide incomplete information.

Genetic variability for micronutrients under an aerobic condition in local landraces of rice from different agro-ecological regions of Karnataka, India

Abstract. Thuy NP, Nhu TTT, Trai NN, Nhu NK, Thao NHX, Phong VT, Khoa BD, Luan NT. 2023. Genetic variability for micronutrients under an aerobic condition in local landraces of rice from different agroecologicalregions of Karnataka, India. Biodiversitas 24: 1-10.Genetic variability is a fundamental reference for the innovative development of breeding programs to improve varieties and desirable traits. This study aimed to assess genetic variability for micronutrients (iron and zinc) and protein content under an aerobic condition in local landraces of rice (Oryza sativa L.) from different agroecological regions of Karnataka, India. Fifty-fivelocal landraces of ricefrom India were studied.High phenotypic and genotypic coefficient of variation was observed for grain yield per plant and moderate for the remaining traits. Heritability was high for most of the traits observed while moderatefor harvest index and grain yield plant-1. The genetic advance (GA) was high for the majority of yield and its attributing traits. GA as mean was high for grain yield per plant. Based on D2value, 50 genotypes were grouped into 8 clusters. Clusters VI and VIII are the largest and had accessions with the highest protein and zinc content. Marabatta, Jeerige Batta, Neerguli, Mugadh Sughanda, and Kesarnellu genotypes contained high grain protein and zinc content, whereas Naga Batta genotype has high iron content. Among the superior nutrient landraces, Neerguli, Jeerige Batta, DBN, Kesarnellu, and Doddiga genotypes had high grain yield plant-1.

Sustainable Intensification of Cassava Production towards Food Security in the Lomami Province (DR Congo): Role of Planting Method and Landrace

Cassava is a mainstay crop for food security in Africa, its tubers being a large source of carbohydrates for the human diet. In some regions (e.g., the Democratic Republic of the Congo; DRC), leaves are also consumed as a source of proteins, vitamins, and minerals. Cassava adapts well to a range of soil-climate conditions and requires low inputs, yet yields are often unsatisfactory because of failures in disseminating improved genotypes and agricultural practices. The aim of this study was to test the effect of (i) seedbed preparation for planting cassava (i.e., flat, mounds, and ridges) and (ii) local landraces (i.e., Kakuanga, Kasongoy, Kasonie, Ndunda, and Ngoymuamba) on yield components and their nutritional quality in the Lomami province (DRC). In-depth measurements of yield components were performed, including the number of tubers and stems per plant, leaf biomass, stem biomass, root yield, and peeling yield. Tubers and leaves were also analyzed for chemical composition. Our results demonstrated that mound and ridge seedbed preparations may highly increase tuber yield (+32–68%) compared with flat. This is not the case for leaves and stems, which were not affected. The Ngoymuamba landrace showed a tuber yield about three times larger than Ndunda, which represented the common productivity values (5–8 Mg ha−1). No effect of seedbed preparation was observed and only minor differences between landraces were observed for the chemical composition of roots and leaves. We concluded that selecting the best-performing seedbed preparations × landraces could have a significant potential for achieving in a relatively short time the goal of “Zero Hunger” and improving the diet in the DRC.

Assessment of drought Associated Characters in Red Sorghum (Sorghum bicolor) Germplasm and Land Races

From the farmer’s field of Krishnagiri and Dharmapuri districts fourteen local land races of sorghum were collected and sixteen germplasm accessions from the Department of Plant Genetic Resources, TNAU, Coimbatore along with two promising sorghum varieties Paiyur 1 and Paiyur2 were taken for study and characterization was done for six drought tolerant traits. Among the 32 land races evaluated, Sorghum land races 16-05 (RS), 2457, 16-01 (RS), 2657, 4269 were found to be early in (82-85 days), high yielding (28 to 29.3g/plant), having high photosynthetic rate, (38.6 umolm2 s-1), high Proline content (394 ugg-1) and high soluble protein content (14.4 mgg-1) and hence found to be tolerant to drought. These sorghum land races can be used as one of the parents in crossing programme for developing short duration, high yielding drought tolerant varieties in sorghum.

Evaluation of Mustard Genotypes for Terai Regions of Nepal

Mustard is locally popular with the name of tora/rayo/raichi is getting priority among the farmers due to its dual advantage of leafy vegetables and oilseed. With the objective identification of the potential mustard genotypes from the available gene pools, a coordinated Varietal Trial (CVT) was conducted in Oilseed Research Program, Nawalpur. A total of 14 mustard genotypes were evaluated in a randomized complete block design (RCBD) with three replications. The plot size was 10 m2 with five rows of 3-meter length maintaining inter-row of 40 cm and intra-row spacing of 15 cm. While depicting the yield, ICJ01-11 was considered the highest yielding genotype with the average yield of 521 kg/ha followed by ICJ9708 with the yield of 467 kg/ha and RH30 with the yield of 450 kg/ha. Due to the pivotal role of mustard as an oilseed production, it is indispensable to evaluate the available gene pool of local landrace, varieties and foreign genotypes of mustard to find out the highest yielding. So these promising genotypes can be used the in future to develop varieties of genotypes with high yield.

Trait Association and path coefficient analysis for yield and yield attributing traits in Sesame (Sesamum indicum L.)

Correlation and path analysis were carried out in 36 diverse genotypes of sesame collected from different parts of the country along with North-East region of India for local landraces. Analysis of variance indicated the presence of good amount of genetic variability among the genotypes under study. Correlation studies indicated that seed yield per plant was significantly and positively associated with traits, i.e. plant height, internodal length, number of capsules per plant, number of branches per plant, stover yield per plant and harvest index both at genotypic and phenotypic levels which indicate the importance of these traits in selection for enhancing the seed yield. Path analysis revealed that maximum positive direct effect on seed yield per plant was imposed by days to 80% physiological maturity, internodal length, capsule length, stem height from base to first branch, stover yield per plant, harvest index, relative water content, oil content and protein content both at genotypic and phenotypic levels. This indicate that these are the real independent characters and they have maximum contribution towards increase in the seed yield per plant.

Evaluation of F1 Crosses of Brinjal (Solanum melongena L.) Landraces for Fruit Yield, Quality and Tolerance to Bacterial Wilt

Field experiment was carried out at All India Coordinated Research Project on Vegetable Crops, OUAT, Bhubaneswar, Odisha, India in order to identify the superior F1 crosses for marketable fruit yield quality and resistance to bacterial wilt. All total 21 F1 crosses evolved from seven distinctly diverse local landraces of brinjal along with a hybrid check, Mahy Green were evaluated by adopting Randomized Block Design and replicated twice in rabi 2021-2022. Results revealed significant variations among various fruit quality attributes (TSS: 4.32 oBrix to 6.00 oBrix and ascorbic acid content of fruit: 5.10 mg100g-1 to 7.10 mg100g-1), incidence of bacterial wilt (30 DAT: 0.00% to 4.17%, 60 DAT: 0.00% to 12.50% and 90 DAT: 0.00% to 20.83%). Out of 21 crosses, nine cross showed immune reaction to bacterial wilt at 90 DAT. The marketable fruit yield plant-1 varied significantly from 1.00 kg to 1.99 kg. The F1 cross viz. BBSR-08-2 × Selection from BBSR-145-1(1.99 kg) recorded significantly highest fruit yield plant-1 followed by BBSR-08-2 × BBSR-10-25(1.87 kg) and BBSR-08-2 × BBSR-10-26 (1.81 kg). Thus, it may be concluded that, F1 crosses obtained from local landraces of brinjal viz., BBSR-08-2 × Selection from BBSR-145-1, BBSR-08-2 × BBSR-10-25 and BBSR-08-2 × BBSR-10-26 may be recommended for higher marketable fruit yield, fruit quality and over all resistance to bacterial wilt for higher profit. These local landraces may also be used for future brinjal improvement programme towards development varieties with higher fruit yield quality and resistance to bacterial wilt.

Development of homozygous maize lines differing in oil and zein content using in-vivo maternal haploid technique

This study was carried out in order to develop homozygous lines that differ in grain quality from the local maize population. Twelve different local maize landraces were used as donor materials in the study. These populations were subjected to induction crossing under greenhouse conditions in September 2020 with the ADAIL-I inducer line. In September 2021, a total of twelve haploid lines were grown in greenhouse conditions. Some plant traits and some grain quality characteristics were examined. Zein protein fractions were also analyzed with SDS-PAGE analysis. The haploid induction rates (HIR) of donor materials ranged from 6.08% to 11.71%. The average HIR value of the ADAIL-I inducer line was determined as 8.20%. The average value of plant height of developed lines varied between 123 cm and 250 cm; first ear height between 54 cm and 120 cm; stem diameter between 0.7 cm and 1.2 cm; crude oil content between 2.39%, and 7.54%; oleic acid content between 15.34% and %30.98; linoleic acid content between 50.4% and 67.8%; protein content between 6.75% and 13.74%; and zein content between 4.58%, and 5.04%. Some the homozygous lines carry the desired protein bands in terms of zein fractions.

Resistance to Pythium root rot and anthracnose among Kenyan common bean genotypes and marker-assisted introgression of resistance genes

ABSTRACT Common bean (Phaseolus vulgaris L.) is an important food legume crop and major protein source in Kenya and many parts of the world. However, the yields are low due to the diseases like anthracnose caused by Colletotrichum lindemunthianum and Pythium root rots. The study, therefore, sought to screen Kenyan common bean genotypes for anthracnose and Pythium resistance and to improve the resistance of local landraces to the two diseases. Local landraces and commercial cultivars were screened for resistance under greenhouse conditions for Pythium and in vitro for anthracnose. The results showed that majority of the landraces were moderately resistant to anthracnose and Pythium root rot. The study further utilized molecular markers linked to anthracnose and Pythium resistance, respectively, to determine their potential to detect specific resistance genes among the test genotypes. None of the markers amplified the test genotypes except the control genotypes pointing toward different resistance genes. Further, cultivars G2333 and KK8 were used as donor parents for anthracnose and Pythium resistance, respectively, in a marker-assisted backcross breeding program involving three high-yielding farmer-preferred landraces. A number of lines were developed that carry both resistance genes. These lines can be advanced for release as new cultivars to enhance common bean production in Kenya.

Growth, yield and quality of selected sweet potato (Ipomoea batatas [l.] Lam.), lines under varying soil moisture conditions

Sweet Potato (Ipomoea batatas [L.] has increasing potential as a food security crop in Kenya. However, its’ production is relatively low compared to its potential production which is attributed to drought conditions alongside use of local landrace cultivars that are quite low yielding. A study was conducted at Meru University of Science and Technology in Kenya in 2020-2021 to assess the performance of selected improved sweet potato lines under varied watering regimes. Two selected sweet potato varieties were used; CIP 106988.1 (Naspot13) and CIP 112286.1(Margarette), and a farmer-preferred variety Kemb-10. The varieties were subjected to three watering regimes (i) Severe stress (SS), 4 WAE (ii) Moderate Stress (MS), 9 WAE and (iii) well watered to maturity. A randomized complete block design in a split plot, with water treatment as the main plot and varieties as subplot with 3 replicates was used. There was no significant interaction between moisture treatments and varieties. Water treatments significantly (p≤0.05) affected vine length, number of leaves and tuber yield. Plants under severe stress had significantly lower vine length, branches and leaves. Under Moderate stress 9 WAE, plants experienced significant reduction in vine length, leaves and branches compared to the well watered. However, this reduction was less compared to severe stress. The reduced vine length and tuber yield is attributed to reduced cell division, elongation and assimilates partitioning. Tuber numbers correlated with leaves (r = 0.63*, 0.74*), and vine length (r = 0.62*, 0.76*). This positive correlation implies that if drought significantly reduces number of leaves and vine length, it reduces tuber yield indicating effect of drought on tuber yield can be estimated by measuring tuber length and number of leaves. Based on findings, ensuring the crop obtains enough moisture during the first nine weeks after planting is recommendable whereas Naspot13 is suitable for the area considering its higher productivity.

Symbiotic Performance and Characterization of Pigeonpea (Cajanus cajan L. Millsp.) Rhizobia Occurring in South African Soils

Pigeonpea (Cajanus cajan (L.) Millsp.) is an important grain legume, which, like several other legumes, depends on the process of biological nitrogen fixation for its nitrogen (N2) requirement by forming a symbiotic association with rhizobia. Compared to other tropical legumes, however, the productivity of pigeonpea in South Africa is low, despite the extensive interests in developing it for wider markets. To assist this process, the objectives of the current study were to (i) characterize putative indigenous rhizobial strains that were previously derived from local soils with no previous history of legume cultivation and (ii) confirm their nodulation abilities on a local landrace and a genetically improved (exotic) genotype of pigeonpea. DNA-based analyses using the 16S rRNA and recA genes showed that the strains predominantly represented Rhizobium and Bradyrhizobium, although we also recovered Phyllobacterium and Paraburkholderia. These rhizobia nodulated both the local landrace and the improved pigeonpea genotype that were included for comparative purposes. In many cases, rhizobia performed similarly on the two genotypes, although the locally sourced landrace mostly performed better in terms of nodulation and plant biomass. While the current study generated vital information regarding the diversity of indigenous rhizobia associating with pigeonpea, further screening (including field inoculation trials) would be necessary to identify possible elite nitrogen fixing rhizobial strains for development as inoculants to enhance South African pigeonpea production.