Abstract Objective To compare patient knowledge, attitudes and behaviour regarding antibiotic use between 2000 and 2005. Method Postal survey to 400 patients in March-April 2005. Setting Two general medical practices in Grampian, UK. Key findings There was a 64% response rate; 90% had experience of antibiotic use and 96% were happy to take antibiotics when necessary; 75% felt we should be more careful about how we use antibiotics. Compared to 2000, fewer patients indicated that most infections clear up by themselves without the need for antibiotics (48% versus 59%, P = 0.031). Fewer of those who experienced at least one respiratory tract infection (RTI) in the review period had consulted a doctor (2005, 14% versus 2000, 23%), and fewer consultations resulted in an antibiotic being prescribed (55% versus 75%). Fewer patients now agreed that telephone helplines for advice on RTIs are a good idea (42% versus 54%, P = 0.009), while more felt that people should make more use of their local pharmacist for advice on treatments for RTI and that they, themselves, would consult a pharmacist. On advice (from doctors, pharmacists or advertisements) on the management of RTI, fewer patients now cited: drink fluids (48% versus 68%, P = 0.023); rest (35% versus 54%, P = 0.012); take paracetamol (35% versus 50% P = 0.028); while more cited: self-medicate (non-paracetamol) (32% versus 15%, P = 0.004); let infection run its course (11% versus 5%, P = 0.027). Conclusions Antibiotic use remains a concern for many patients; however, almost all would take antibiotics when necessary. Fewer patients now consult a doctor about a RTI, and fewer consultations lead to an antibiotic being prescribed. Patients are increasingly positive about pharmacist advice but less keen on telephone helplines. A further local health education initiative should be considered to remind patients of strategies for the self-management of RTI.