Palaeolithic archaeological sites of the Western Ukraine are clustered along the Prut and Dniester Rivers. Different sites provided data enabling reconstruction of the paleoenvironment, chronology and cultures of human group during the Upper Paleolithic (notably Molodova V). During the second part of the Pleniglacial, between 23 000 and 20 000 years BP, palaeoclimatic variations took place. The intensification of cold and arid conditions is liable to force human groups to adapt to a changing environment. Little is known about this period, with only a few assemblages. Ongoing excavations continue to provide new data. The archaeological site of Dorochivtsy III has an important sequence stratigraphy with several archaeological layers. Among the seven Upper Palaeolithic layers, layers 6, 5, 4 and 3 testify to activities of a human group during the Upper Pleniglacial. We studied the faunal assemblages applying zooarchaeological methods, identifying mammoth (Mammuthus primigenius), reindeer (Rangifer tarandus), horse (Equus sp.) and fox (Alopex lagopus/Vulpes vulpes). The Gravettian layers 3, 4, 5 have anthropogenic clues in connection with subsistence activities oriented on reindeer, then horses, and finally fox. Concerning mammoths, we cannot define the modes of acquisition and use. These occupations have been interpreted as recurrent hunting occupations linked to procurement of local flint and lithic production for butchering activities. Layer 6 dated to 22 330 ± 100 BP is remarkable because of previously unseen practises. The lithic assemblage combined with bone industry and engraved tusk is a novel set of cultural elements in this area, called ancient Epigravettian. This layer testifies to the diversity of human activities during the Upper Pleniglacial and to the particular status of mammoth ivory as an artistic medium. Palynological data and taphonomic observations on bones indicate the persistence of relatively moist conditions during some periods, which could favour the movement of human groups. Although little is known about the Upper Pleniglacial period, Dorochivtsy III testifies to the continuity of a large exploitation of the territory of the Dniester valley.