Local Birds Research Articles

Newcastle disease virus genotype VII.1.1 identified from backyard chickens with low antibody titer: Jimma Zone, Southwest Ethiopia.

BackgroundNewcastle disease significantly impacts the global poultry industry and is prevalent in many African countries, including Ethiopia. The objective of this research is to determine the humoral immune response to Newcastle Disease Virus (NDV), identify the circulating NDV genotype, and evaluate the correlation between the diagnostic tests used in backyard chickens in the Jimma Zone, southwest Ethiopia.MethodsA total of 90 swab and blood samples were purposively collected from symptomatic backyard chicken in the period between February and April 2022. Samples were collected from Jimma town, Seqa Chekorsa and Tiro Afeta districts of Jimma zone. Enzyme linked immunosorbent assay (ELISA) was conducted and seropositivity was determined from the collected serum samples. From the swab samples, total RNA was extracted and the viral genomic material was detected by amplifying the Fusion gene by Reverse transcription polymerase chain reaction (RT-PCR). The interconnection between ELISA and RT-PCR was also analyzed. Further, positive swab samples were nucleotide sequenced and genotyped.ResultsOf the 90 serum samples, 62 (68.8%) were seropositive. From the 90 swab samples, 14 (15.5%) were RT-PCR positive. No statistically significant association between risk factors (breed, age, sex) and virus exposure was observed by RT-PCR (P = 0.41, 0.44, 0.67) or ELISA (P = 0.85, 0.19, 0.11). However, local breeds, young, and male birds were at higher risk according to RT-PCR results, while young and female birds were more likely to be seropositive. The antibody titer study showed that RT-PCR positive birds produced less than half the mean antibodies of negative birds (x̄=854 vs. 1885) and positive birds produced similar amount of antibody (σ = 626). Local (x̄=1978), adult (x̄=2558), and female (x̄=2620) birds had higher mean antibody titers than their counterparts. The agreement between RT-PCR and ELISA in identifying positive samples was minimal (k = 0.05). Nucleotide-sequenced isolates were nearly identical (99.7%) to each other and identified as velogenic based on the F-gene cleavage site (RRQKRF) with genotype VII.1.1.ConclusionNDV was circulating in the study area, infecting birds with low antibody titers. High viral similarity between neighboring countries emphasizes the need for regional disease control strategy.

Vulnerable bird species and avian mortality due to predation by domestic cat (Felis catus) in anthropogenically transformed landscapes of the Bukovynian Carpathians

Abstract The domestic cat (Felis catus) poses a significant threat to bird fauna, particularly in the urbanised, anthropogenically transformed landscapes of the Bukovynian Carpathians, Ukraine. This study aimed to determine the annual bird mortality rate due to cat predation and identify vulnerable species to this threat. Using the Monte Carlo method with 10,000 iterations, mortality rates were calculated. Data were gathered from sociological surveys, field observations, and scientific publications. The methodology included estimating the number of rural pets and feral cats across 877.9 km2, calculating population density, and assessing bird predation. Annually, an average of 509,000 birds are killed, with a 95% mortality estimate range between 188.6 thousand and 1,008.6 thousand, which highlights the cats’ impact on local bird populations. Linear regression models were developed to predict bird mortality based on cat population size. The regression formulae are nBFC = 44.10 * nFC for feral cats and nBRP = 32.92 * nRP for rural pets. A total of 62 vulnerable bird species were identified in these landscapes of the Bukovynian Carpathians.

Molecular detection of haemobacteria in Colombian wild birds

Colombia shows a high density and variety of bird species, making it one of the most diverse avian territories globally. Antioquia ranks among the top four provinces with the greatest variety of bird species, underscoring the importance of research efforts on the local bird fauna. Therefore, this study aimed to identify bacterial agents in the blood of wild birds from the municipality of Jardín (Antioquia, Colombia) by 16S qPCR sequencing. A descriptive cross-sectional study was conducted using non-probabilistic convenience sampling. Wild birds were captured using mist nets and blood samples were collected from each animal via puncture using sterile lancets in the brachial vein, and a drop of blood was collected on filter paper for qPCR analysis. The 16S gene in bacterial genomes was found in 13 out of 46 wild birds of the Passeriform and quasi-Passeriform orders, captured at three different locations within the study municipality at altitudes ranging from 1,665 to 2,034 m.a.s.l. Seven different bird species were recorded and four different haemobacteria were identified (i.e. Exiguobacterium spp., Escherichia coli, Stenotrophomonas spp., and Stenotrophomonas maltophilia). This study contributes to the knowledge in Colombia by identifying four different hemobacteria in wild birds. Further research is required on the health status of these birds and the attributable impacts on their populations and other related factors, including humans.

Migratory birds have a distinct haemosporidian community and are temporally decoupled from vector abundance at a stopover site.

Migratory animals likely play an important role in the geographic spread of parasites. In fact, a common assumption is that parasites are potentially transmitted by migratory animals at temporary stopover sites along migratory routes, yet very few studies have assessed whether transmission at stopover sites can or does occur. We investigated the potential for a group of vector-transmitted parasites, the avian haemosporidians, to be transmitted during migratory stopover periods at Rushton Woods Preserve in Pennsylvania, USA. Using an analysis of 1454 sampled avian hosts, we found that while a core group of abundant haemosporidians was shared between local breeding birds and passing migrants, the parasite community of migratory birds at Rushton was distinct from that of local breeding birds and showed similarity to a previously sampled boreal forest haemosporidian community. Haemosporidians that were unique to passing migratory birds were associated with sampling sites in North America with cooler summer temperatures than haemosporidians that are transmitted at Rushton, suggesting that the transmission of these parasites may be restricted to high-latitude regions outside of our temperate stopover site. We also found that the abundance of mosquitoes in our study region is offset from that of migratory bird abundance during avian migratory periods, with the peak period of bird migration occurring during periods of low mosquito activity. Collectively, these findings suggest that although abundant haemosporidians are possibly transmitted between local and passing migratory birds, a combination of biotic and abiotic factors may constrain haemosporidian transmission during avian stopover at our study site.

Serological detection of Toxoplasma gondii in local duck birds, and Gallus gallus in Basrah Province, Iraq

تنتشر المقوسة الكوندية على نطاق واسع في البشر والحيوانات الأخرى، بما في ذلك الدواجن، في جميع أنحاء العالم وتعتمد على مجموعة فريدة من العضيات الإفرازية والهيكلية الخلوية لغزو الخلايا المضيفة. يتم استهلاك لحوم الدواجن المصابة بالمقوسة الكوندية والتي تشمل الدجاج والبط على نطاق واسع في معظم البلدان وهي معروفة كمصدر رئيسي للعدوى البشرية. في العراق بشكل عام وفي محافظة البصرة على وجه الخصوص، نادرًا ما يُعرف عن انتشار المقوسة الكوندية في البط والدجاج المحلي. في الدراسة الحالية، تم استخدام اختبار تلازن اللاتكس (LAT) ومجموعة الاختبار السريع للاجسم المضادة IgM/IgG لفحص الأجسام المضادة لطفيلي المقوسة الكوندية في 40 طائر من البط محلي و 62 دجاج محلي من مناطق مختلفة في محافظة البصرة. تم العثور على الأجسام المضادة لـلطفيلي (LAT) في 14 (35٪) من 40 طائر بط محلي ، و 23 (37.09٪) من 62 من الدجاج المحلي. بينما تم الكشف عن الأجسام المضادة للطفيلي (الاختبار السريع) في 10 (25٪) من 40 طائر بط محلي و 17 (27.41٪) من 62 من الدجاج المحلي. أظهرت النتائج أن التربة ملوثة بالكيسات البيضية للطفيلي التي تمثل مصدراً غذائياً للدجاج والبط المحلي نظرا لتغذية الطيور على الأرض. وتشير الدراسة إلى أن لحوم الدواجن قد تكون مصدراً مهماً للعدوى البشرية بالطفيلي في العراق. تعتبر هذه الدراسة هي الدراسة الأولى في محافظة البصرة للكشف عن داء المقوسات في الدجاج والبط المحلي.

Effects of wind turbine dimensions on the collision risk of raptors: A simulation approach based on flight height distributions

Wind energy development is a key component of climate change mitigation. However, birds collide with wind turbines, and this additional mortality may negatively impact populations. Collision risk could be reduced by informed selection of turbine dimensions, but the effects of turbine dimensions are still unknown for many species.As analyses of mortality data have several limitations, we applied a simulation approach based on flight height distributions of six European raptor species. To obtain accurate flight height data, we used high-frequency GPS tracking (GPS tags deployed on 275 individuals). The effects of ground clearance and rotor diameter of wind turbines on collision risk were studied using the Band collision risk model.Five species had a unimodal flight height distribution, with a mode below 25 m above ground level, while Short-toed Eagle showed a more uniform distribution with a weak mode between 120 and 260 m. The proportion of positions within 32–200 m ranged from 11 % in Marsh Harrier to 54 % in Red Kite.With increasing ground clearance (from 20 to 100 m), collision risk decreased in the species with low mode (−56 to −66 %), but increased in Short-toed Eagle (+38 %). With increasing rotor diameter (from 50 to 160 m) at fixed ground clearance, the collision risk per turbine increased in all species (+151 to +558 %), while the collision risk per MW decreased in the species with low mode (−50 % to −57 %).These results underpin that wind turbine dimensions can have substantial effects on the collision risk of raptors. As the effects varied between species, wind energy planning should consider the composition of the local bird community to optimise wind turbine dimensions. For species with a low mode of flight height, the collision risk for a given total power capacity could be reduced by increasing ground clearance, and using fewer turbines with larger diameter.

Broad geographic variation in age- and sex-dependent origin of harvested eurasian wigeon (Mareca penelope) revealed by stable-hydrogen (δ2H) isotope analyses of feathers

Migratory waterfowl are a harvested resource shared among multiple European countries, exposing them to potential overexploitation. Management of take is challenging since the life cycle of migratory waterfowl consists of several stages distributed among several locations, with possible spatio-temporal overlap among populations with differing population trends. Successful harvest management in such situations requires knowledge about the connections between breeding and non-breeding locations, and where birds are harvested. Breeding populations of Eurasian wigeon (Mareca penelope) are declining in Finland, underlining the need for more effective harvest management. Relative proportions and temporal distribution of local breeding birds and migrants from a larger Russian breeding population within the Finnish hunting bag has been unknown to date. We studied spatio-temporal origins of Finnish harvested wigeon by measuring stable-hydrogen (δ2H) isotope values from legally harvested birds. We modelled the changes in δ2H values of the feather samples within the hunting season using Gaussian processes and found that the origin of harvested wigeon in Finland changed during the hunting season and differed by age and sex. In juveniles and adult females but not in adult males, origin of harvested birds shifted from local and possibly western Russian birds to more long-distance migratory birds during the harvest season. These patterns likely reflected sex- and age-specific differences in migratory behaviour of Eurasian wigeon in the East Atlantic flyway, which can be used to guide future management and conservation of this species through the implementation of spatio-temporal harvest regulation.

Diversity and Conservation of Wetland Avifauna in Nanjarayan Lake, Tiruppur, Tamil Nadu, India

This paper shares the results from a big bird survey at Nanjarayan Lake in Tamil Nadu. It ran from 2022 to 2024. To check out the bird variety, see how it changes with the seasons and look at how well different bird species are doing there. Over three years, we did lots of field surveys during different times of the year. We used methods like point counts, transect walks, and habitat checks. In total, we found 184 bird species resident birds that live there all year and migrating ones that visit. We even spotted some species that need special attention. This shows how important the lake is for both local birds and those just flying between. When we looked closely at the numbers through the seasons, we found some big changes in how many birds there were. Winter was especially busy, with a lot of different kinds showing up and the migratory birds arriving. The study also identified key threats to avian biodiversity in the lake, such as habitat degradation, water pollution, and human disturbances. Based on these findings, the study recommends urgent conservation measures, including habitat restoration, pollution control, and the implementation of strict regulations to minimize human impact. This survey underscores the importance of Nanjarayan Lake as a vital bird habitat and calls for enhanced conservation efforts to protect its rich avian diversity. The findings provide valuable baseline data for future ecological studies and contribute to the broader understanding of lake ecosystems in Tamil Nadu.

Architectural traditions in the structures built by cooperative weaver birds.

Humans cooperate to build complex structures with culture-specific architectural styles. However, they are not the only animals to build complex structures nor to have culture. We show that social groups of white-browed sparrow weavers (Plocepasser mahali) build structures (nests for breeding and multiple single-occupant roosts for sleeping) that differ architecturally among groups. Morphological differences are consistent across years and are clear even among groups with territories a few meters apart. These repeatable differences are not explained by among-group variation in local weather conditions, bird size, tree height, or patterns of genetic relatedness. Architectural styles are also robust to the immigration of birds from other groups.

Changes in Danish bird communities over four decades of climate and land‐use change

Our understanding of how human activities impact biodiversity comes largely from space‐for‐time substitutions. However, spatial gradients are a poor surrogate for changes through time as they do not account for dynamic processes such as delayed extinction debts. Here we contribute towards filling this research gap by assessing the trajectories of local avian assemblages over 40+ years of climate and land‐use change. Using four decades of volunteer observations in Denmark we investigated long‐term trends of local bird richness, community structure, function, abundance, and biomass to better understand their anthropogenic drivers. Between 1976 and 2020, volunteers recorded ~ 2.4 million birds at 378 routes spanning a median of 15 years (range: 10–44). At the local level, we found a restructuring of bird communities over time (6% change per decade) and declines in abundance (−7% per decade), but stability in biomass, functional diversity, and spatial turnover. Local species richness showed a shallow decline on average. These results provide evidence that temporal turnover and loss of individuals are the most prominent features of recent ecological change in these communities. We found that the rate of local warming was positively associated with trends of species richness and functional diversity, suggesting a potential redistribution of warm‐adapted species. Meanwhile, communities that were becoming more spatially homogenous were associated with urban and farmland areas. In space, environmental changes are often distinct and recognisable, e.g. between forest and farmland. Through time, however, changes can be infrequent, gradual, and non‐linear. Despite these challenges, our results illustrate the power of spatially replicated, long‐term biodiversity monitoring programs for detecting the trends and attributing drivers of local biodiversity change.

Bird-Ecosystem Relationship in an Urbanizing Habitat: Implications for Conservation and Ecosystem Stability

This study explores the association between birds and their urbanizing ecosystems. The study area was divided into 4 quadrats of area 2700 m2 each. Bird species as well as number of individuals of each species were counted using the Line Transect Method. Statistical analysis of the data was performed using Windows Microsoft Excel and SPSS v.28. The investigation revealed a significant correlation (P < 0.05) between bird species and the four distinct sites within the study area. These sites exhibit variations in vegetation composition and include notable features like water bodies. The findings underscore the heavy reliance of certain bird species in this region on specific types of vegetation for their survival and well-being. Removing even a single plant species could potentially disrupt the entire ecosystem. The study documented the presence of 42 bird species across 30 families, including one vulnerable species. Both local and seasonal migratory birds were observed during the research. Although the study identified a decline in bird diversity within the study area, it remains species-rich, displaying moderate to high diversity (Simpson’s Dominance Index: 0.0319; Margalef’s Richness Index: 4.0754). This research emphasizes the critical importance of conserving bird species and their associated vegetation to maintain ecosystem integrity. Strategic sustainable development practices along with habitat enrichment for birds is recommended to maintain the valuable avifaunal diversity of the study area. By highlighting the intricate relationship between birds and their habitat, the study highlights the significance of conservation efforts in safeguarding the overall health of the ecosystem.

Commons are cared for: Coexistence between humans and wild birds on the island of Kihnu, Estonia

Abstract Local knowledge is an essential dimension of humanity's relationship with the environment. Investigating the interdependence between humans and wild birds holds value in fostering a mutually beneficial relationship with avian populations. Gathering bird eggs is a significant practice for Kihnu islanders in Estonia. There are very few studies of this age‐old, complex knowledge that assess the topic from the perspective of the local community. This paper investigates the reciprocity and the relationality between people and birds and the position of commons in the local community practice of bird semi‐domestication. Several methods (interviews, participant observation and literature study) were used in data collection. We obtained information on local knowledge concerning 58 bird taxa. Locals named at least 21 bird species from which they have collected eggs. While collecting eggs is a central activity, the relationship surrounding egg collection is far more intricate. Locals take care of nesting boxes in Common Merganser which birds reside, collecting only a few eggs from every box. These nesting boxes are also constructed for passerine birds. Furthermore, caring for birds influences the soundscape of Kihnu and significantly impacts the cultural bond between local communities and the birds. Kihnu islanders are mindful of the changes occurring in the bird population. The intimate relationship between local people and birds is challenged when traditional ways of life are restricted by stricter nature conservation norms (e.g. banning the collection of bird eggs and visiting islets). However, birdlife is very important in ensuring biocultural diversity. Therefore, this study proposes several important future perspectives in promoting the sustainable development of rural life: (1) the introduction of pluralistic nature protection, including the opinion of local communities in the management of natural resources; (2) the promotion of birds as a means of pest control; and (3) reinforcing the reciprocal coexistence of birds and humans on Kihnu. We advocate for community‐centred nature management which takes into consideration Local Ecological Knowledge and its attached expertise, shared responsibility and benefits, sustainable development trajectories and local community‐based governance systems. Read the free Plain Language Summary for this article on the Journal blog.

Anatolia: A Hotspot of Avian Genetic Diversity in the Western Palaearctic

Located at the crossroads of two continents and at the southeastern edge of the Mediterranean Basin, Anatolia was one of the most important Pleistocene glacial refugia in the Western Palaearctic. As part of the Irano-Anatolian, Caucasus and Mediterranean Basin biodiversity hotspots, this region is also home to a rich avian community including nearly 400 breeding species. Nevertheless, research addressing the genetic structure and diversity of local bird populations is limited, and information on glacial refugia in this region is still scant, especially when compared to other large Mediterranean peninsulas, namely the Balkan, Italian and Iberian ones. In this study, we contribute to filling this gap by addressing the biogeographic pattern of four common resident songbirds—the Eurasian blue tit (Cyanistes caeruleus), the great tit (Parus major), the Eurasian chaffinch (Fringilla coelebs) and the Eurasian blackbird (Turdus merula)—and one endemic species—the Krüper’s nuthatch (Sitta krueperi)—by amplifying two mitochondrial DNA genes in individuals from Anatolia (n = 329) and comparing their sequences to those of conspecifics from the rest of their distribution range across the western Palaearctic (n = 357) deposited in public databases. The overall genetic structure of these species is consistent with a scenario of isolation for multiple populations in different refugia across Anatolia and subsequent secondary contact in the wake of ice retreat, which makes this region a hotspot of genetic diversity for both widespread and endemic avian species.

Molecular Survey of Haemosporidian Parasites in Procellariiformes Sampled in Southern Brazil, 2013-22.

The order Procellariiformes includes several species of seabirds that perform long-distance migrations crossing all the oceans. These movements may contribute to the dispersal and exchange of hemoparasites, such as haemosporidians. There is a lack of studies regarding the order Haemosporida in Procellariiformes, and, to date, only the genus Plasmodium has been reported. This survey investigated the occurrence of the three genera of haemosporidians, Plasmodium, Haemoproteus, and Leucocytozoon, in samples collected between 2013 and 2022 from 95 individuals of 14 species of Procellariiformes from southern Brazil, including live animals in rehabilitation centers, individuals caught as incidental bycatch, and carcasses found along the coast. A total of 171 samples of blood and fragments of liver and spleen were analyzed, with extracted DNA being subjected to a nested PCR followed by phylogeny analysis. All animals were negative for Plasmodium spp. and Leucocytozoon spp., but one Black-browed Albatross (Thalassarche melanophris) and one Manx Shearwater (Puffinus puffinus) specimen were positive for Haemoproteus spp. The sequences obtained from positive seabirds did not show 100% similarity with other known lineages available in the MalAvi database and thus were probably novel lineages. However, one sequence clustered together with Haemoproteus noctuae, a parasite from Strigiformes, while the other was grouped with Haemoproteus columbae, which is classically related to Columbiformes. These results suggest that both positive animals may have become infected when beached or in rehabilitation centers by a spillover of vectors from local birds. This highlights the importance of surveillance of the health of Procellariiformes regarding the possibility of dissemination of new pathogens in different bird populations.

Effects of Invasive Smooth Cordgrass Degradation on Avian Species Diversity in the Dafeng Milu National Nature Reserve, a Ramsar Wetland on the Eastern Coast of China

Invasive smooth cordgrass (Spartina alterniflora) has been expanding rapidly through the coastal wetlands of eastern China and these changes negatively affect local birds. In the Dafeng Milu National Nature Reserve (henceforth referred to as DMNNR), rapid degradation of spartina occurs after an increase in milu (Elaphures davidianus; hereafter elk) numbers and ecological hydrological engineering. We evaluated the impact of such degradation on the abundance and species diversity of birds in the DMNNR during 2017–2021. We found that the area covered by S. alterniflora decreased significantly in the study area at a rate of 310 ha per year and by 62% during 2017–2021 (p < 0.01). With this decrease in the S. alterniflora area, the species richness and abundance of birds first increased and then decreased. Songbird density clearly decreased but species richness did not significantly do so. This research demonstrated that during the initial stages of vegetation degradation, there was a positive effect on bird diversity. With the increasing vegetation degradation increases, both songbirds and waterbirds experience negative impacts. The DMNNR is an important stopover site for waterbirds in the East Asian–Australasian Flyway, and additional measures are needed to control vegetation degradation and to restore the native habitats for birds.

Local and regional-scale effects of hedgerows on grassland- and forest-associated bird populations within agroecosystems.

Linear woody features (LWFs), like hedgerows along field edges, provide wildlife habitat and support biodiversity in agroecosystems. Assessments of LWFs usually focus on community-level indices, such as species richness. However, effective conservation actions need to balance the contrasting habitat preferences of different wildlife species, necessitating a focus on population-level effects in working landscapes. We assessed associations between LWFs and abundance for 45 bird species within an intensive agroecosystem in eastern Ontario, Canada. We used distance- and removal-sampling methods across 4 years (2016-2019) to estimate local bird abundance in habitats representing a range of LWF densities. We also predicted abundance across a subset of the study region with and without LWFs to understand their contribution to regional population density. Associations between local bird abundance and LWFs were variable among species, but overall community effects were clearly positive, particularly for forest and shrubland species. At the site level, 20/45 species (44%) had higher densities associated with greater LWF presence on average, compared to 5/45 (11%) with negative associations. At the regional scale, LWFs had predicted benefits on total abundance for 31 species (69%), contributing to an estimated 20% increase on average. Positive effects were most pronounced in areas with greater agricultural land use (primarily field crops), suggesting LWFs may provide crucial habitat in heavily modified landscapes but have little to no additional benefit for the avian community in areas with greater existing heterogeneity and habitat retention. Species that responded negatively tended to be at risk with strong habitat preferences for intact forests or large, open grasslands and, thus, greater sensitivity to potential edge effects. With rapidly declining songbird populations and a global need for food security, conservation strategies that amplify biodiversity and enhance agricultural productivity through ecosystem services such as pest control, pollination, and water regulation are vital. We demonstrate the benefits of habitat heterogeneity in agroecosystems on songbird densities and highlight the need to integrate local and landscape-level assessments in conservation planning. An effective, balanced strategy includes concentrating LWFs in areas of extensive arable crops, with habitat retention patches where possible, while maintaining heterogeneity through mixtures of natural habitats and pastoral farming in less intensive regions.

Assessing Water-Saving Technologies and the Impact of Giant Tortoise Herbivory on the Restoration of Opuntia megasperma var. orientalis on Española Island—Galapagos

The prickly pear (Opuntia megasperma var. orientalis), a pivotal species for the ecological balance of Española Island in Galapagos, has witnessed a severe decline in its population due to the enduring presence of introduced feral goats over several decades. Additionally, the inherent slow recovery of this species, requiring several years of development, has contributed to its population decline. Several attempts were carried out to restore this species, but they were not successful due to the island’s extreme arid conditions. Subsequently, innovative water-saving technologies were introduced to ensure the survival and growth of the Opuntia species. Two water-saving technologies, Groasis Waterboxx® and Hydrogel, were applied in two distinct treatments, the first involving Waterboxx solely, and the second combining Waterboxx with Hydrogel, alongside a control group. Planting involved two types of cacti: cladodes and seedlings. To safeguard against potential damage from giant tortoises and local birds, protective mesh fencing was installed around the plants. Each monitoring session recorded plant survival and growth, evaluating the impact of water-saving technologies on cactus survival, maximum plant height reached, age at the time of plant death, and growth achieved since planting. Additionally, the study assessed the influence of climate on plant survival and growth. Unfortunately, the employment of protective mesh fences and Waterboxx containers resulted in the unintended loss of specific bird species. Consequently, a decision was taken to remove these protective measures, resulting in a substantial rise in herbivorous activity, and the subsequent mortality of nearly all plants. Our findings underscore the efficacy of water-saving technologies in Opuntia restoration. However, successful application necessitates a better understanding of these technologies within the unique conditions of the island. Future endeavors should focus on refining these techniques to minimize avian mortality while fostering biodiversity and restoring ecological equilibrium.

Targeted genomic sequencing of avian influenza viruses in wetland sediment from wild bird habitats.

Diverse influenza A viruses (IAVs) circulate in wild birds, including highly pathogenic strains that infect poultry and humans. Consequently, surveillance of IAVs in wild birds is a cornerstone of agricultural biosecurity and pandemic preparedness. Surveillance is traditionally done by testing wild birds directly, but obtaining these specimens is labor intensive, detection rates can be low, and sampling is often biased toward certain avian species. As a result, local incursions of dangerous IAVs are rarely detected before outbreaks begin. Testing environmental specimens from wild bird habitats has been proposed as an alternative surveillance strategy. These specimens are thought to contain diverse IAVs deposited by a broad range of avian hosts, including species that are not typically sampled by surveillance programs. To enable this surveillance strategy, we developed a targeted genomic sequencing method for characterizing IAVs in these challenging environmental specimens. It combines custom hybridization probes, unique molecular index-based library construction, and purpose-built bioinformatic tools, allowing IAV genomic material to be enriched and analyzed with single-fragment resolution. We demonstrated our method on 90 sediment specimens from wetlands around Vancouver, Canada. We recovered 2,312 IAV genome fragments originating from all eight IAV genome segments. Eleven hemagglutinin subtypes and nine neuraminidase subtypes were detected, including H5, the current global surveillance priority. Our results demonstrate that targeted genomic sequencing of environmental specimens from wild bird habitats could become a valuable complement to avian influenza surveillance programs.IMPORTANCEIn this study, we developed genome sequencing tools for characterizing avian influenza viruses in sediment from wild bird habitats. These tools enable an environment-based approach to avian influenza surveillance. This could improve early detection of dangerous strains in local wild birds, allowing poultry producers to better protect their flocks and prevent human exposures to potential pandemic threats. Furthermore, we purposefully developed these methods to contend with viral genomic material that is diluted, fragmented, incomplete, and derived from multiple strains and hosts. These challenges are common to many environmental specimens, making these methods broadly applicable for genomic pathogen surveillance in diverse contexts.

Effects of Partial Replacement of Soybean with Local Alternative Sources on Growth, Blood Parameters, Welfare, and Economic Indicators of Local and Commercial Broilers.

The effects of the partial replacement of soybean with alternative local agri-industry by-products and black soldier fly (BSF) larvae meal on broiler growth performance, blood biochemistry, welfare, and, subsequently, economic performance of these diets were evaluated. A total of 524 day-old chicks from a local and a commercial strain were fed one of the three diets from the day of hatch to the slaughter age. The diets were the following: a soybean-based control diet, a diet in which soybean was partially replaced (SPR) with agri-industrial by-products, or a diet with BSF larvae meal added to the SPR (SPR + BSF). There was no effect of the diets on the slaughter weight, total feed consumption, and feed conversion of the chickens. The SPR + BSF diet reduced the blood glucose, alanine aminotransferase, aspartate aminotransferase, gamma-glutamyl transferase, protein, triglycerides, and cholesterol levels in the local chickens and the gamma-glutamyl transferase, protein, and creatinine levels in the commercial broilers. The negative effect of the SPR diet on plumage cleanliness in the commercial broilers was alleviated by the SPR + BSF diet, whereas 100% of the local birds presented either slight or moderate soiling. The results showed that, due to the high cost of the BSF larvae meal, the SPR + BSF diet was not economically feasible. In a further study, the price trends of BSF larvae will be examined from the standpoint of economic profitability conditions.

Avian Diversity in and around Wetland of National Importance ‘Pariyej Community Reservoir’, Gujarat, India

The Pariyej Community Reservoir (PCR) is a freshwater reservoir in the Kheda district of Gujarat State, India. The PCR is also listed in Asian Directory of Wetlands. It is an important water storage reservoir; falls in the 4-B Gujarat Rajwara region of Central Gujarat, India. The present study was carried out for the period of one year (March, 2021 to February, 2022) to assess the status of avian diversity in terms of its species richness, abundance, and density. 116 bird species from 20 orders, 51 families, and 100 genera were documented. 75 species (64.4%) were resident, whereas 41 (35.3%) were migratory or seasonal. With respect to abundance, 41species (35.34%) were abundant, 39 species (33.6%) common, and 36 rare species (31.0%). The maximum total abundance (1707 individuals) was found at Site 1 in winter season with mean abundance of 427 individuals, total density (114 individuals/sq.km)and mean density (29 individuals/sq.km). The minimum total abundance (288 individuals) was recorded at Site2 in monsoon season with mean abundance of 72 individuals, total density (19individuals/sq.km) and mean density (5 individuals/sq.km).The present investigation reflects the good species richness of avian specieswith respect to order: family: genera: species ratioi.e., 1: 2.55: 5.0: 5.8. This indicates the flourishing harboring capability of PCR during peak winter period, which could be due to sustenance of rich biotic elements. The PCR has been declared as Sanctum sanctorumfor the refuge of migratory as well as local migrant birds, which is proved to be a wetland of national importance.