This paper analyses MOZAIC (Measurements of Ozone aboard Airbus in-service airCraft) measurements of carbon monoxide (CO) profiles over Bangkok to discuss the seasonality in vertical distribution during year 2005–2006. The mixing ratios of CO were enhanced in the lower troposphere being highest in winter followed by summer and wet seasons. During all the seasons, the mixing ratio of CO decreased rapidly and remained low in the middle troposphere. At higher altitudes (6–12 km), CO shows enhanced values particularly during wet and early winter seasons. The strong seasonality in CO was caused by the seasonal shift in the patterns of the long-range transport and biomass burning (BB) in South and Southeast Asia (S–SE Asia). Flow of cleaner air and negligible BB resulted in the lowest mixing ratio of CO in the wet season. In addition to anthropogenic influence, the long-range transport and BB caused the higher CO in the winter and summer seasons, respectively. Despite extensive local BB activities in Thailand during the summer season, the moderate levels of CO were attributed to the dilution due to flow of cleaner marine from the Indian and Pacific Oceans. We have also compared the observations with the Model for Ozone And Related Chemical Tracers (MOZART) simulations. Mostly the observations lie between the MOZART-2 and MOZART-4 simulations as they underestimate and overestimate the observed CO, respectively. In the middle and upper troposphere, both the observed and simulated mixing ratios of CO during September–November of year 2006 were higher by 15–30 ppbv compared to the same period of year 2005. Our analysis indicates the impact of El Niño induced extensive BB in Indonesia during the year 2006.