Dynamic excitation in early-age significantly impacts the performances of mature concrete in underground structures, such as tunnels beneath railways supported by cast-in-place concrete. This study explores the impact of early dynamic excitation on properties of mature concrete. Vibration tests at a fundamental frequency of 114Hz, with peak acceleration levels ranging from 0.1g to 0.4g, were conducted using shaking table. Specimens underwent 33seconds of dynamic excitation every 15minutes over the initial 48hours, excluding a night break from 0-6am, totaling 114 vibration cycles. Various lab tests were employed to assess the properties and microstructure of mature concrete post-vibration, including water penetration resistance, capillary water absorption, Coulomb electric flux, scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP) and Nitrogen adsorption-desorption measurement (NAM). The results proves that vibration causes water secretion and air bubbles discharge before the initial setting of concrete, thereby enhancing the hydration reaction. The relationship between vibration loads with varying peak accelerations and the cohesion resulting from hydration reaction influences the microstructural evolution of concrete, leading to the weakening and strengthening of permeability of mature concrete. Under the vibration spectrum and test conditions described in this paper, vibration loads with peak values less than 0.234g decrease the total volume of large and middle pores (>20nm) by up to 50% and increase the total volume of small pores (<20nm) by up to 200%. Due to these changes in pore structure, the permeability coefficient (Sk), cumulative capillary water height (i) and Coulomb electric flux decrease by up to 47.84%, 39.5% and 31.6%, respectively. However, when the amplitudes of vibration loads exceed 0.234g, the total volume of large and medium pores (>20nm) increases, and that of small pores (<20nm) decreases, leading to a reduction of the impermeability of mature concrete. Additionally, the SEM analysis corroborates these microstructural variations.