In this study, the effect of mixed-mode loading on fatigue crack growth under varying applied loads for SAPH440 material was experimentally investigated. Previous research has either examined the effect of mixed-mode loading at a fixed angle or its effect on slanted cracks. This paper uses a CTS specimen and a loading device to apply varying angles of load during Mode I loading conditions. Initially, a numerical analysis was performed to validate existing equations for the stress intensity factor, which quantifies the crack driving force in the experiments. In the crack initiation region, load shedding techniques and load increasing techniques were used to confirm fatigue cracking considering mixed-mode effects. The change in the threshold value according to the loading angle was also evaluated. In the crack propagation region, a multi-level loading technique was employed to assess the mixed-mode effects under varying loading angles. This process verified the mixed-mode effect under conditions where the applied load angle varies. The fatigue crack fracture surfaces were examined using digital camera capture and SEM. It was found that when mixed-mode loading occurs during Mode I loading, the fatigue crack propagation rate varies depending on the mixed-mode loading angle.