Livestock Losses Research Articles

Assam Flood and Financial Loss in Agricultural Production: A Study on Mitigation Strategy Adopted by the Farmers of Dhemaji District

Background: Assam is considered as a flood prone state, as this natural disaster creates devastating situation almost every year. The study was conducted in Dhemaji district of Assam which is in the list of highly flood hazard index. The district was selected to know the impact of flood on financial loss for agricultural production and to study the mitigation strategy adopted by the flood affected farmers in the district. Methods: For that a multistage random sampling technique was adopted to select flood affected farmers from the district. Financial loss for agricultural production due to flood was estimated by calculating financial loss for crop loss, agricultural asset loss and livestock loss for the study area. Flood situation and Non flood situation was compared while going for calculation of financial loss for crop due to flood. Henry Garret ranking technique was adopted to rank the problems face by the farmers. Result: The result reflected that net return from crop production was recorded to be less (Group I 1032.44Rs/ha and Group II 3830.99 Rs/ha) in flood situation than the non-flood situation (Group I 7051.30 Rs/ha and Group II 8330.08 Rs/ha).Total financial loss due to crop loss, asset loss and livestock loss was found to be high for size group II farmers (Rs 39094.33) then the size group I (Rs 36643.40). Majority of the respondents (79.17 per cent) preferred migration as the best coping mechanism to protect them from flood loss.

Tanshinone IIA protects intestinal epithelial cells from deoxynivalenol-induced pyroptosis

Deoxynivalenol (DON) is the most common mycotoxin in food and feed, which can cause undesirable effects, including diarrhea, emesis, weight loss, and growth delay in livestock. Intestinal epithelial cells were the main target of DON, which can cause oxidative stress and inflammatory injury. Tanshinone IIA (Tan IIA) is fat-soluble diterpene quinone, which is the most abundant active ingredient in salvia miltiorrhiza plant with antioxidant and anti-inflammatory characteristics. However, it is not clear whether Tan IIA can protect against or inhibit intestinal oxidative stress and inflammatory injury under DON exposure. This study aimed to explore the protective effect of Tan IIA on DON-induced toxicity in porcine jejunum epithelial cells (IPEC-J2). Cells were exposed to 0, 0.5, 1.0, 2.0 µM DON and/or 45 µg/mL TAN ⅡA to detect oxidative stress indicators. inflammatory cytokines, NF-κB expression, NLRP3 inflammasome and pyroptosis-related factors. In this study, DON exposure caused IPEC-J2 cells oxidative stress by elevating ROS and 8-OHdG content, inhibited GSH-Px activity. Furthermore, DON increased pro-inflammatory factor (TNF-α, IL-1β, IL-18 and IL-6) expression and decreased the anti-inflammatory factor (IL-10) expression, causing inflammatory response via triggering NF-κB pathway. Interestingly, above changes were alleviated after Tan IIA treatment. In addition, Tan IIA relieved DON-induced pyroptosis by suppressing the expression of pyroptosis-related factors (NLRP3, Caspase-1, GSDMD, IL-1β, and IL-18). In general, our data suggested that Tan IIA can ameliorate DON-induced intestinal epithelial cells injury associated with suppressing the pyroptosis signaling pathway. Our findings pointed that Tan IIA could be used as the potential therapeutic drugs on DON-induced enterotoxicity.

Testing a conservation compromise: No evidence that public wolf hunting in Slovakia reduced livestock losses

AbstractVariation in the legal status and management of wolves (Canis lupus) across EU Member States provides a good opportunity to test the effectiveness of different practices to reduce livestock losses. This opportunity for testing is particularly useful for lethal interventions, as they are among the most controversial actions within the large carnivore management toolbox. We aimed to test a conservation compromise adopted in Slovakia, based on a public wolf‐hunting scheme and annual hunting quotas between 2014 and 2019, and partially justified to reduce livestock losses. We assessed whether this hunting scheme influenced livestock depredation levels (at the district level). Wolves in the area fed mainly on wild ungulates (98.9% of consumed biomass). While domestic sheep comprised only 0.5% of the diet, they were dominant among the reported livestock killed by wolves (91.1%). Using two different approaches, we did not observe a relationship between the number of killed wolves and livestock losses. Alternatively, a negative relationship between wild prey biomass and livestock losses was found. Since 2021, public wolf hunting has not been conducted in Slovakia, and there is no merit in the previous justification for this conservation compromise to reduce livestock losses.

Study on Human Wildlife Conflict and Its Impact on The Livelihood of Rural Communities of Andhra Pradesh, India

The escalating human-wildlife conflicts in rural communities, driven by expanding human populations encroaching upon natural habits, pose significant risks to both human safety and livelihoods as highlighted in the WWF Magazine ‘s Spring Issue (2023). These conflicts result from differing perspectives on resolution, leading to tensions among various groups and causing a reduction in territory, natural resources, fatalities, injuries and habitat destruction for both humans and wildlife. Unfortunately, some rare and endangered species face the threat of extinction due to human action taken to prevent future conflicts, emphasizing the urgent need to address this issue. Philip J. Nyhus (2016) suggests that a range of social and psychological factors, including cultural backgrounds, emotional connections, economic considerations governance structures and stakeholder involvement influence people’s interaction with wildlife. Based on the data obtained from various sources around2020-23, the number of animal and financial losses was estimated. Most of the damage is caused in Srikakulam, Vizianagaram, Parvathipuram, and Eluru districts where most of the HWC occurs mainly due to elephant attacks (Elephas spp), along with monkeys (Maccacca spp), wild Boar (Sus spp), Snakes like (Naja naja, Krait, Bungarus spp) Mice, Mouse, Rat spp., Jackles (Canis spp), Tiger (Tigris spp) which is amounting to almost 22% of the livestock loss domesticated by farmers. The domesticated animals which were mostly eaten by the predators are Fishes, (Catla, Labeo,Cirhinus spp.) Birds (Gallus, Chittagong, Aseel spp.) goats (Capra spp.), sheep (Ovis spp,) Cows (Bos spp.), pigs (Sus), Buffalo (Bubalus spp.), etc. Altogether substantial amount of monetary loss has occurred. The public and government officials in combined action can mitigate these problems from future attacks. To address this issue, effective management strategies are needed that balance the needs of both humans and wildlife while minimizing the risk of conflict.

Diet of Black-backed Jackal (Canis mesomelas, Schreber, 1775), impacts on livelihood and perceptions of farmers in Konasa Pulasa community conserved forest, omo valley of Ethiopia

BackgroundLivestock depredation by the black-backed jackal (Canis mesomelas) occurs widely across Africa. The study on human-jackal conflict is important for conservation efforts in Ethiopia. The aim of this study was to investigate the diet of black-backed jackals, to understand their predation effects on domestic livestock and perceptions of farmers’ in the Konasa-Pulasa Community Conserved Forest, Omo Valley of Ethiopia. The study was conducted using scat analysis, questionnaire survey and Focus Group Discussion methods. Livestock depredation and the economic impact of farmers were assessed among 290 randomly selected households. A total of 90 scat samples were collected and analyzed during the dry and wet seasons to identify the diet of jackals.ResultsA total of 624 domestic animals have been lost in the last 5 years (2016–2020). The estimated economic cost of domestic animals lost due to predation by jackals was US $18,180.0 in the last five years, and US $12.5 per year per household. The major diet composition of jackals was of domestic animal origin (45.5%), followed by wild animals (30.8%) in both the dry and wet seasons. However, more prey diversity was recorded during the wet season. The respondents revealed that the causes of black-backed jackal conflict in the study area were higher due to increasing jackal population size (40%). The major traditional mitigation method was guarding (42%). Most of the respondents (48.2%) had negative perceptions towards the conservation of black-backed jackals.ConclusionLivestock depredations by black-backed jackals were the major issue of conflict in the study area. Scat analysis showed that large percentage of domestic animal species remain in the scat of jackals. Livestock losses caused by jackals represent an economic concern for livestock owners in the area. Local people close to the forest boundary were highly vulnerable to domestic animal loss due to predation by jackals. Therefore, improved livestock husbandry methods will be implemented by the local people for effective jackal conservation in Konasa-Pulasa Community Conserved Forest. Understanding the ecological and social dimensions of conflict situations in the area may have important ecological and management implications for the country.

Catharanthus roseus extract attenuates E. coli toxin-induced short circuit current in isolated jejunal epithelium of goat and buffalo

Enterotoxigenic E. coli-derived heat-stable enterotoxin not only alters intestinal barrier functions and produces secretory diarrhea but also causes production losses in livestock. This study aimed to elucidate the ameliorative effect of Catharanthus roseus extract on Cl- secretion in an in vitro model. Isolated jejunal epithelia from goats (N=10) and buffaloes (N=4) were divided into four groups: A) control; B) 1.5% C. roseus extract; C) 10% E. coli toxin, and D) 10% E. coli toxin and 1.5% C. roseus extract. The jejunal epithelia were mounted on Ussing chambers with an exposed surface area of 0.95 cm2 and treated with E. coli toxin on the mucosal side to invoke Cl- channels. Transepithelial potential difference (Pdt), tissue conductance (Gt), and short circuit current (Isc) were determined under voltage-clamped conditions. E. coli toxin produced a significant increase in the Pdt, Gt, and Isc for both goat and buffalo jejunal epithelia, whereas C. roseus extract on the mucosal side alone or followed by the E. coli toxin decreased these parameters, which were comparable with the control. The change in Isc was more pronounced in goats than buffaloes (ΔIscgoat > ΔIscbuff). In experiments with the goat jejunal epithelia, replacement of Cl- from the mucosal buffer decreased Isc, similar to a decrease observed for the C. roseus extract groups. In conclusion, the results of this study suggest that C. roseus extract has a potential to attenuate E. coli-induced Cl- secretion in isolated jejunal epithelia of goats and buffaloes.
 Keywords: Catharanthus roseus extract; Electrophysiology; Secretory diarrhea; Small intestine, Ruminants

Dynamics and determinants of human–hyaena conflicts in the surroundings of a protected area

AbstractHuman–wildlife conflicts (HWC) predominate in areas proximal to wildlife rich protected zones. Whereas herbivore driven HWC is easy to detect, the stealth, nocturnal and predacious nature of carnivores leads to human injury and fatality, livestock depredation and loss of livelihoods in communities proximal to Sengwa Wildlife Research Area (SWRA) in northwest Zimbabwe. This study aimed to: (1) determine the nature of human–hyaena conflicts (HHCs), (2) explore the drivers of HHCs and (3) investigate the spatial and temporal movement patterns of hyaenas in HHC hotspots in areas proximal to SWRA. The results indicated that prevalent HHC types include livestock depredation, property damage, harassment, nuisance and disruption of human movements around SWRA. Locals indicated removal of park fences, human settlement on wildlife migration corridors, increases in hyaena populations, easy stray livestock targets, increased poaching and removal of natural wildlife death carcasses by illegal hunters as key drivers of HHC. Hyaenas have a predetermined non‐seasonal movement pattern following traditional wildlife migration corridors through SWRA into proximal communities. Human–hyaena conflict abatement strategies should be underpinned by indigenous local knowledge of hyaena movement and hunting patterns. Integrating citizen science data and remote sensing techniques aids to track and understand movement and hunting patterns of hyaenas inside and outside of protected areas.

Serosurvey of selected reproductive pathogens in domestic ruminants from Upper Egypt

Toxoplasmosis, neosporosis, and Q fever are among the most important abortifacient diseases in ruminants worldwide. These diseases result in huge economic losses in livestock besides the fact that some of are of public health concern. The present study aimed to update the data about the current seroepidemiological situation of these diseases in Upper Egypt. A total of 411 blood samples were collected from small and large ruminants and serologically tested against the presence of T. gondii, N. caninum, and C. burnetii. Generalized estimating equation (GEE) models were performed to assess the potential risk factors associated with the exposure to these pathogens. The overall seroprevalence of T. gondii was 47.9% (197/411) with an individual seropositivity of 59.4% (63/106), 58.6% (17/29), 38.8% (54/139) and 46% (63/137) in cattle, buffalo, sheep and goats, respectively. Meanwhile, 9.7% (38/411) of the examined animals were tested positive for anti-N. caninum antibodies, with an individual seropositivity of 13.2% (12/106), 34.5% (10/29), 8.6% (12/139) and 2.9% (4/137) in cattle, buffalo, sheep and goats, respectively. Furthermore, the overall prevalence of antibodies against C. burnetii was 17.3% (63/411), and exposure to this pathogen was detected in 4.7% (5/106) of cattle, 19.3% (20/129) of sheep, 29.2% (38/130) of goats but none of the examined buffalo were found to be seropositive. A total of 12.1% (50/411) of the examined animals showed co-exposure to at least two of the tested pathogens. Regarding the potential risk factors, there were statistically significant differences among species in the frequency of exposure to the three tested pathogens. Age (> 6 months) was also shown to be a significant risk factor associated with T. gondii exposure. The results obtained provided updated information about the occurrence of three of the main reproductive pathogens in Upper Egypt. The high seropositivity values found for the tested zoonotic pathogens in most of the analyzed ruminant species suggest the necessity of performing additional in-depth studies to evaluate the epidemiology of these pathogens in the study area.

Metabarcoding using nanopore long-read sequencing for the unbiased characterization of apicomplexan haemoparasites.

Apicomplexan haemoparasites generate significant morbidity and mortality in humans and other animals, particularly in many low-to-middle income countries. Malaria caused by Plasmodium remains responsible for some of the highest numbers of annual deaths of any human pathogen, whilst piroplasmids, such as Babesia and Theileria can have immense negative economic effects through livestock loss. Diagnosing haemoparasites via traditional methods like microscopy is challenging due to low-level and transient parasitaemia. PCR-based diagnostics overcome these limitations by being both highly sensitive and specific, but they may be unable to accurately detect coinfections or identify novel species. In contrast, next-generation sequencing (NGS)-based methods can characterize all pathogens from a group of interest concurrently, although, the short-read platforms previously used have been limited in the taxonomic resolution achievable. Here, we used Oxford Nanopore Technologies' (ONT) long-read MinION™ sequencer to conduct apicomplexan haemoparasite metabarcoding via sequencing the near full-length 18S ribosomal RNA gene, demonstrating its ability to detect Babesia, Hepatozoon, Neospora, Plasmodium, Theileria and Toxoplasma species. This method was tested on blood-extracted DNA from 100 dogs and the results benchmarked against qPCR and Illumina-based metabarcoding. For two common haemoparasites, nanopore sequencing performed as well as qPCR (kappa agreement statistics > 0.98), whilst also detecting one pathogen, Hepatozoon felis, missed by the other techniques. The long-reads obtained by nanopore sequencing provide an improved species-level taxonomic resolution whilst the method's broad applicability mean it can be used to explore apicomplexan communities from diverse mammalian hosts, on a portable sequencer that easily permits adaptation to field use.

DEVELOPMENT OF SHEEP FARMING AS A PRIORITY BRANCH OF AGRICULTURE IN THE ZABAIKALSKY REGION

The pedigree base of sheep farming in the Zabaikalsky region is concentrated in 8 pedigree farms and nine reproducer farms, as well as in the Autonomous Region "Zabaikalsky Center for Pedigree Livestock Farming." Currently, there are seven pedigree farms and six reproducer farms in the region that are engaged in breeding Zabaikalsky fine-fleeced sheep, with a total population of 76.9 thousand sheep, accounting for 75.7% of the total pedigree sheep population. An analysis of the sheep population in the region over 15 years indicates a decline, which can be attributed to reduced demand for wool production and livestock losses due to fires that affected large areas. It should be noted that wool production has decreased due to cheaper raw materials such as cotton and synthetics. The region has adopted a Comprehensive Program for the Development of Sheep Farming until 2030, the result of which should be an increase in the sheep population from 405 to 655 thousand head, wool production from 964 to 2173 tons, the creation of at least 1,500 new jobs in sheep farming and the processing industry, and the development of high-quality clothing and other consumer products based on wool by the end of 2030. Scientific research justifies the priority development of sheep farming in the region as a traditional branch of agriculture and a tool for preserving the rural way of life. Since sheep farming products are primarily concentrated in private households, where difficulties in selling products are observed, developing a model for interaction between these entities and agricultural organisations, pedigree farms, and reproducer farms is advisable.

Livelihood risks impact livestock reduction behavior of herders: evidence from Inner Mongolia, China

We analyzed the primary livelihood risks faced by livestock-herding households across six counties and three grassland types in Inner Mongolia. The major livelihood risks were natural, market, financial, and policy. These risks are influenced by changes in stocking rate (SR), which are critical for controlling livestock numbers and averting risks associated with overgrazing in natural grasslands. We propose a correction method for SR based on hay purchase from a survey of 450 herders. We compared the distribution of households’ SR after the correction and used a multiple linear regression model to empirically test the effect of livelihood risks on SR. The empirical model passed robustness test and the regression results of variables were robust. SR declined across grassland types after hay purchases. The SR of the meadow steppe was reduced by 35%, typical steppe by 23%, and desert steppe by 32%. Various factors affected changes in SR, including ecological subsidies to household income, timely access to market information, and annual livestock losses. We discuss implications of our findings for policy, market transactions, livestock insurance, social security, and formal credit systems in pastoral areas.

Q fever and toxoplasmosis in South African livestock and wildlife: a retrospective study on seropositivity, sporadic abortion, and stillbirth cases in livestock caused by Coxiella burnetii

BackgroundQ fever and toxoplasmosis are economically important zoonoses as they cause considerable losses in livestock (cattle, sheep and goats) and wildlife (antelopes, giraffes, lions, and cheetahs) through reproductive disorders such as abortions and stillbirths. Q fever and toxoplasmosis testing in South Africa is conducted by the Agricultural Research Council-Onderstepoort Veterinary Research (ARC-OVR). However, both zoonoses are understudied and not monitored in South Africa as they are not considered controlled or notifiable diseases in the Animal Disease Act 35 of 1984. A retrospective study was conducted on Q fever (2007–2009) and toxoplasmosis (2007–2017) using diagnostic laboratory data at the ARC-OVR. Also, we report on sporadic abortion and stillbirth cases in livestock from diagnostic tissue samples submitted for Coxiella burnetii polymerase chain reaction (PCR) detection at the ARC-OVR.ResultsDuring 2007 to 2009, 766 animal samples were tested for C. burnetii antibodies and seropositivity was 0.9% (95%CI: 0.3–1.7) with sheep (1.9%; 95%CI: 0.6–4.4) having the highest seropositivity followed by cattle (0.7%; 95%CI: 0.09–2.6), while all goats (0.0%; 95%CI: 0.0–4.2) and wildlife (0.0%; 95%CI: 0.0–2.5) tested were negative. From 2007 to 2017, 567 sera were tested for T. gondii antibodies; overall seropositivity was 12.2% (95%CI: 9.6–15). Wildlife had highest seropositivity to T. gondii antibodies (13.9%; 95%CI: 9.0–19.7) followed by goats (12.9%; 95%CI: 9.2–17.4) and sheep (12.3%; 95%CI: 5.1–23.8) while seropositivity in cattle was 2.4% (95%CI: 0.06–12.9). Of 11 animals tested by C. burnetii PCR detection (2021–2022), 10 (91.0%) were positive. The amplicon sequences showed similarity to Coxiella burnetii strain 54T1 transposase gene partial coding sequence.ConclusionsWe have confirmed the occurrence of the causative agents of Q fever and toxoplasmosis in livestock and wildlife in South Africa, with data limitations. These zoonoses remain of importance with limited information about them in South Africa. This study provides baseline information for future studies on Q fever and toxoplasmosis in South African livestock and wildlife, as well other African countries. Due to limited data collection experienced in this study, it is recommended that improvements in data collection samples tested should include associated factors such as sex, age, and breed of the animals.

Contextualizing carnivores illegal hunting and opportunities for livestock-predator coexistence from nonlethal practices and ranchers' attitudes

Illegal hunting is a pervasive menace for many carnivore species in livestock-wildlife shared lands. Carnivore conservation under such socio-ecological context demands a comprehensive understanding of hunting drivers as well as alternatives for problem resolution. To contribute overcoming retaliation against carnivores we developed a holistic approach to address ranchers' non-compliance by understanding together: the context, magnitude, effectiveness, and motivations underlying illegal hunting. We surveyed sheep ranches across southern Chilean Patagonia where culpeo fox, chilla fox and puma cooccur and prey upon sheep. Sheep depredation occurred in 85 % ranches, accounting for 3.5 % of total holdings. Other sources of sheep mortality emerged equally sizeable or even higher than predation. Culpeo fox was the main predator across the region, and the most persecuted. Although hunting was significantly related to its perceived effectiveness, livestock guardian dogs conversely were asserted as the most effective tool for protecting the herds. Ranches where hunting is applied suffered significantly more predation than those where LGDs are deployed. Personal norms, perception towards carnivores, damage levels and ranchers' characteristics significantly related to hunting likelihood, yet their effects varied between species. Our approach advances in demonstrating clear opportunities for livestock-carnivores coexistence arising from a comprehensive understanding of the full context of the predation-retaliation problem, and the multiple drivers of hunting in a species-by-species basis. Such opportunities lie fundamentally in acknowledging all sources of livestock loss, the encouragement to adopt LGDs, and fostering pro-carnivore attitudes based on the ecological benefits they provide in rangelands.

Livestock Depredation and Its Impact on Farmers in Trongsa District, Bhutan

This survey was conducted in the Trongsa District of Bhutan to assess and document the depredation of livestock by wild predators, time, and seasonality of predation, estimate the economic value of livestock losses in three years (2019-2021), and assess farmers’ perception of livestock depredation. A survey using a semi-structured questionnaire was done with 218 farming households from five subdistricts of Trongsa, Bhutan. From a total of 683 livestock kills recorded, the tiger (69.69%) killed more than the dhole (14.4%), leopard (13.7%), and bear (1.4%) in the last three years. Adult cattle (milch, Dry & Bull) were more vulnerable (n=496) compared to young (calf & heifer) cattle (n=169). The majority of predation was in summer (51.18%) and winter (23.62%) whereas the rests (25.19%) were believed to have occurred irrespective of seasons. The total loss of 683 livestock head was valued at 380,739.13$ of which the majority were cattle (n= 665, 345,219.69$) followed by yak (n=48, 35,032.87$). Each household on average lost approximately 23.8% of the annual household cash income in the last three years and only 13% (n=30) received some form of compensation. The majority of respondents (96.8%) attribute a current increase in depredation cases to wildlife conservation efforts such as strict conservation laws, protected areas, and biological corridors. We recommend livestock intensification programs such as pasture improvements in fallow lands and financial compensations for the loss of improved breeds as short-term measures. We recommend for enhanced discussion on livestock insurance schemes and involve farmers in managing human-wildlife conflicts.

Cattle ranchers' tolerance to jaguar revealed through a spectrum of intended behaviours across a gradient of losses in a gamified survey-based field experiment

Promoting tolerance towards wildlife that pose a threat to humans or their livelihoods is critical to achieving species conservation goals. While conservation discourses often revolve around retaliation, tolerance and intolerance can vary from passive acceptance to active support, and according to level and scale of loss. We developed an experimental tool with a gamified survey-based field experiment to test multiple intended behaviours along a gradient of livestock loss to predation by jaguars (Panthera onca) in Calakmul, Mexico. Using a within-subject design, participants played a card-choice game against livestock loss scenarios. The choices offered were “No action [taken]”, “Protect [domestic animals]”, “Kill [the jaguar]”. First, our experimental design was validated by increased frequencies of “Kill” responses in reaction to hypothetical jaguar incursion with increasing damage. The greatest difference occurred between zero and minimal damage (one chicken), showing that whether damage occurs at all is at least as important as its extent. Secondly, average frequency of intentional retaliation to jaguar incursion was higher for participants who had already killed a jaguar. Finally, age, education, and ethnicity all correlated with intentional behaviours to implement preventive measure or kill jaguars. Past experience of attack had no effect. Our results showed that survey/game-based experiments can assess tolerance to potentially hazardous wildlife interactions, while allowing more nuanced understandings of people's tolerance on a gradient of loss. This allows us to move away from a dualistic model of “conflict” versus “coexistence” towards a more complex understanding incorporating individual attitudes and experience, cultural significance, and socio-economic context.

Does Livestock Loss Affect Livelihood? An Investigation on Char Residing Mishing Community of Assam

Does Livestock Loss Affect Livelihood? An Investigation on Char Residing Mishing Community of Assam

Development of eco-friendly natural formulation for management of resistant tick infesting animals and humans

Almost all livestock species are suffering from tick infestations. Ticks are voracious blood suckers, causing significant blood loss in livestock resulting low productivity in terms of weight gain and milk yield, increased mortality and low-quality hides. Ticks also serve as the major vector of economically important diseases like babesiosis, theileriosis and anaplasmosis. The use of synthetic acaricides of different classes is the backbone of tick management programme. But indiscriminate application of these chemicals has led to the development of resistance against commonly used synthetic acaricides. It is become a challenging task to manage tick infestations using existing resources. The present study was undertaken to develop green nano formulation for mitigation of the resistant problem. The characterized green nano-formulation may be a suitable alternative to minimize the excessive use of chemical acaricides. The use of synthetic acaricides of different classes is the backbone of tick management programme. But the indiscriminate application of these chemicals has led to the development of resistance against commonly used synthetic acaricides.

Wide variation of heterozygotic genotypes of recent fasciolid hybrids from livestock in Bangladesh assessed by rDNA internal transcribed spacer region sequencing and cloning

Fascioliasis causes high economic losses in livestock and underlies public health problems in rural areas, mainly of low-income countries. The increasing animal infection rates in Bangladesh were assessed, by focusing on host species, different parts of the country, and rDNA sequences. Fasciolid flukes were collected from buffaloes, cattle, goats and sheep from many localities to assess prevalences and intensities of infection. The nuclear rDNA internal transcribed spacer (ITS) region including ITS-1 and ITS-2 spacers was analyzed by direct sequencing and cloning, given the detection of intermediate phenotypic forms in Bangladesh. The 35.4% prevalence in goats and 55.5% in buffaloes are the highest recorded in these animals in Bangladesh. In cattle (29.3%) and sheep (26.8%) prevalences are also high for these species. These prevalences are very high when compared to lowlands at similar latitudes in neighboring India. The high prevalences and intensities appear in western Bangladesh where cross-border importation of animals from India occur. The combined haplotype CH3A of Fasciola gigantica widely found in all livestock species throughout Bangladesh fits its historical connections with the western Grand Trunk Road and the eastern Tea-Horse Road. The “pure” F. hepatica sequences only in clones from specimens showing heterozygotic positions indicate recent hybridization events with local “pure” F. gigantica, since concerted evolution did not yet have sufficient time to homogenize the rDNA operon. The detection of up to six different sequences coexisting in the cloned specimens evidences crossbreeding between hybrid parents, indicating repeated, superimposed and rapidly evolving hybridization events. The high proportion of hybrids highlights an increasing animal infection trend and human infection risk, and the need for control measures, mainly concerning goats in household farming management. ITS-1 and ITS-2 markers prove to be useful for detecting recent hybrid fasciolids. The introduction of a Fasciola species with imported livestock into a highly prevalent area of the other Fasciola species may lead to a high nucleotide variation in the species-differing positions in the extremely conserved fasciolid spacers. Results suggest that, in ancient times, frequent crossbreeding inside the same Fasciola species gave rise to the very peculiar characteristics of the present-day nuclear genome of both fasciolids.

The influence of superstitions and emotions on villagers' attitudes towards striped hyena in southwestern Iran.

The intensity of human-carnivore conflict in socio-ecological systems may primarily be determined by people's attitudes and perceptions of carnivore-related threats. Direct or indirect threats posed by large carnivores to human interests may eventually lead to negative attitudes that can trigger retaliatory bahaviour against them. We studied local people's attitudes towards striped hyena (Hyaena hyaena), the nature and extent of the human-hyena conflict, and the socio-cultural drivers of the conflicts in 19 rural communities in southwestern Iran. We employed structural equation modelling to assess socio-cultural factors affecting attitudes towards striped hyenas. The findings of 300 interviews showed significant differences in local people's superstitious attitudes regarding gender, age, and education. More than 40% of the participants had encountered hyenas, and on average, each respondent lost 0.44 livestock in the past five years due to hyena attacks. However, livestock depredation by the hyena was low (13.3%) compared to the damage inflicted by all carnivores (73%). While the respondents indicated some degrees of fear, hatred to hyena was relatively low and they generally showed positive attitudes towards the species. Women and older people expressed the highest and respondents with higher education the least superstitious beliefs. Attitude score of respondents toward hyenas was correlated negatively with hatred for hyenas and positively with knowledge about them, but socio-demographics effects on attitudes towards hyenas were not statistically significant. Self-reported livestock loss was a relatively good predictor of hatred and fear. Herders who had not protected their livestock reported carnivore attacks at least once. We conclude that superstitions can potentially negatively affect hyena persistence, but can be reduced by improving the educational level of local people.

Cross-species analysis of transcriptome emphasizes a critical role of TNF-α in mediating MAP2K7/AKT2 signaling in zearalenone-induced apoptosis

Zearalenone (ZEN) is a widespread and transgenerational toxicant that can cause serious reproductive health risks, which poses a potential threat to global agricultural production and human health; its estrogenic activity can lead to reproductive toxicity through the induction of granulosa cell apoptosis. Herein, comparative transcriptome analysis, single-cell transcriptome analysis, and weighted gene co-expression network analysis (WGCNA) combined with gene knockout in vivo and RNA interference in vitro were used to comprehensively describe the damage caused by ZEN exposure on ovarian granulosa cells. Comparative transcriptome analysis and WGCNA suggested that the tumor necrosis factor (TNF)-α-mediated mitogen-activated protein kinase 7 (MAP2K7)/ AKT serine/threonine kinase 2 (AKT2) axis was disordered after ZEN exposure in porcine granulosa cells (pGCs) and mouse granulosa cells (mGCs). In vivo gene knockout and in vitro RNA interference verified that TNF-α-mediated MAP2K7/AKT2 was the guiding signal in ZEN-induced apoptosis in pGCs and mGCs. Moreover, single-cell transcriptome analysis showed that ZEN exposure could induce changes in the TNF signaling pathway in offspring. Overall, we concluded that the TNF-α-mediated MAP2K7/AKT2 axis was the main signaling pathway of ZEN-induced apoptosis in pGCs and mGCs. This work provides new insights into the mechanism of ZEN toxicity and provides new potential therapeutic targets for the loss of livestock and human reproductive health caused by ZEN.