Liver Fluke In Cattle Research Articles

Simultaneous Determination of Five Fasciolicides in Milk by Liquid Chromatography with Electrochemical Detection

A sensitive and specific method is described for determination of 5 fasciolicides in milk. The drugs are used to control liver flukes in cattle. The milk sample was homogenized with acetone and acetonitrile, sonicated, and centrifuged. The supernatant was extracted with dichloromethane. The extract was evaporated to dryness, dissolved in 1% sodium hydrogen carbonate, and purified on a C18 cartridge. The 5 drugs were separated from the matrix by reversed-phase liquid chromatography (LC) and determined by dual-electrode coulometric detection on a Kaseisorb LC ODS-300-5 column. The mobile phase was acetonitrile-0.05M potassium dihydrogen phosphate (55 + 45) at pH 3.0. The flow rate was 1 mL/min at 40 degrees C. The applied potentials of detectors 1 and 2 were set at 0.20 and 0.55 V, respectively. The average recovery of the drugs added to milk at 0.01 and 0.1 micrograms/mL was 89.6%, and the coefficient of variation was 4.7%. The detection limits of the drugs in milk were 4-20 ng/mL. The method is used to monitor commercial milk samples and to determine the residual levels of these drugs in milk from cows treated with a fasciolicide.

The incidence of liver fluke in cattle in central Queensland.

The incidence of liver fluke in cattle in central Queensland.

Production Effects of Liver Fluke in Cattle I. the Effects of Infection on Liveweight Gain, Feed Intake and Food Conversion Efficiency in Beef Cattle

A trial is described in which the effect of infection with Fasciola hepatica on the performance of beef cattle was studied. Liveweight gain, dry matter and energy intakes were examined in relation to two levels of infection and to a superimposed infection. It is concluded from the results that sub-clinical infections derived from 600 metacercariae, which gave an estimated, average infection of 54 fluke per animal 26 weeks after infection, reduced the weight gain by 8% over the first six months of the infection (P < 0 · 05). After six months this infection had little effect on performance. Higher levels of infection (1000 metacercariae) reduced the weight gain by 28% (P < 0 · 05) and caused the appearance of clinical signs in some animals. The superimposed infection generally did not produce as marked a reduction in performance as the same level of infection in previously uninfected animals. However, the reduced weight gain (17%) was still significantly worse than that of the uninfected control cattle (P < 0 · 05). The impaired performance appeared to be due to impaired feed conversion efficiency at the low level of infection, while at the higher infection level, inappetance also contributed to the loss.

Liver fluke in cattle

Liver fluke in cattle

Liver fluke in cattle in the tropics

Liver fluke in cattle in the tropics