Liver B12 Research Articles

Vitamin B12 and biosynthesis in rat liver.

Vitamin B12 and biosynthesis in rat liver.

Liver vitamin B 12 levels in chronic liver diseases.

Range and average values were determined for the vitamin B12 levels of the liver tissues obtained at laparotomy from 35 patients with chronic liver diseases and from 18 patients with peptic ulcer or gastroptosis. In cirrhosis the average value was 0.19μg per g, in cancer of the liver possibly developed from cirrhosis 0.13μg per g, in Banti's syndrome 0.62μg per g, and in peptic ulcer and gastroptosis 0.74μg per g. In cirrhosis and Banti's syndrome the liver B12 levels of the cases receiving parenterally B12 did not rise significantly with a few exceptions, the average values remaining on the similar levels described above.Following the operation of portacaval anastomosis the liver B12 levels of the patients with cirrhosis or Banti's syndrome fell even during parenteral administration of the vitamin. The fall following the operation was not remarkable in cases whose pre-operation levels of the liver B12 were low.

The vitamin B12 content of human liver as determined by bio-assay of needle biopsy material.

Article1 December 1958THE VITAMIN B12 CONTENT OF HUMAN LIVER AS DETERMINED BY BIO-ASSAY OF NEEDLE BIOPSY MATERIALROBERT S. NELSON, M.D., F.A.C.P., VASANT M. DOCTOR, Ph.D.ROBERT S. NELSON, M.D., F.A.C.P.Search for more papers by this author, VASANT M. DOCTOR, Ph.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-49-6-1361 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptThe vitamin B12content of human liver postmortem specimens has been previously studied,1, 8and changes have been noted in pernicious anemia in relapse1and in cirrhosis.8These valuable contributions were necessarily of a static nature, and defined the liver vitamin B12content after death in certain patients who could not be followed by serial determinations during life. Studies with radioactive labeled vitamin B122, 4, 5, 7have done much to clarify the relative contents of various body organs and the rate at which absorption takes place from the bowel and parenterally. It has been shown that, in the human,...Bibliography1. Girdwood RH: The occurrence of growth factors for Lactobacillus leichmannii, Streptococcus faecalis and Leuconostoc citrovorum in the tissues of pernicious anemia patients and controls, Biochem. J. 52: 58, 1952. CrossrefMedlineGoogle Scholar2. GlassBoydGellin GBLJGA: Surface scintillation measurements in humans of the uptake of parenterally administered radioactive vitamin B12 , Blood 10: 95, 1955. CrossrefMedlineGoogle Scholar3. GrosswiczHochmanAronovitchIzakRachmilewitz NAJGM: Malignant growth in the liver and serum-vitamin B12 levels, Lancet 1: 1116, 1957. CrossrefGoogle Scholar4. HeinleWelchScharfMeachanPrusoff RWADVGCWH: Studies of excretion (and absorption) of Co60-labeled vitamin B12 in pernicious anemia, Tr. A. Am. Physicians 65: 214, 1952. MedlineGoogle Scholar5. MeyerBerlinJiminez-CasadoArkun LMNIMSN: Vitamin B12 distribution determined by surface body counting following parenteral administration of Co60 vitamin B12 , Proc. Soc. Exper. Biol. and Med. 91: 129, 1956. CrossrefMedlineGoogle Scholar6. Nelson RS: The development and function of a liver biopsy program: training of personnel, description of a modified Vim-Silverman needle and clinical value of 500 biopsies, Am. J. M. Sc. 227: 152, 1954. CrossrefMedlineGoogle Scholar7. Schilling RF: Recent studies of intrinsic factor and utilization of radioactive vitamin B12 , Federation Proc. 13: 769, 1954. Google Scholar8. SwendseidHvolbollSchickHalsted MEEGJA: The vitamin B12 content of human liver tissue and its nutritional significance, Blood 12: 24, 1957. CrossrefMedlineGoogle Scholar9. ZamcheckSidman NRL: Needle biopsy of liver; its use in clinical and investigative medicine, New England J. Med. 249: 1020, 1953. CrossrefMedlineGoogle Scholar10. ZamcheckKlausenstock NO: Medical progress: liver biopsy; risk of needle biopsy, New England J. Med. 249: 1062, 1953. CrossrefMedlineGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Houston, Texas*Presented at the Thirty-ninth Annual Session of The American College of Physicians, Atlantic City, New Jersey, April 30, 1958.From the Department of Medicine, The University of Texas, M. D. Anderson Hospital and Tumor Institute, Houston, Texas.†This work was supported by grants-in-aid, U. S. Public Health Service, and Institutional Grant from the American Cancer Society.Requests for reprints should be addressed to Robert S. Nelson, M.D., Associate Internist, University of Texas M. D. Anderson Hospital and Tumor Institute, 6723 Bertner, Houston 25, Texas. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byVitamin B12Turnover in ManNEUROLOGICAL SYMPTOMS AND SIGNS IN A SELECTED GROUP OF PARTIALLY GASTRECTOMIZED PATIENTS WITH PARTICULAR REFERENCE TO B12 DEFICIENCYNEUROLOGICAL COMPLICATIONS IN AN UNSELECTED GROUP OF PATIENTS PARTIALLY GASTRECTOMIZED FOR GASTRIC ULCERSurvey of vitamin B12and folate in the serum and marrow tissue of hospitalized patientsLaboratory Tests for the Assessment of Nutritional StatusThe long-term relationship between serum vitamin B12 and total body vitamin B12Observations on Folate Metabolism in Rats on a Vitamin B 12 -Deficient DietThe Content of Vitamin B 12 in Adult and Foetal Tissue: A Comparative StudyDie megaloblastischen AnämienVITAMIN B 12 METABOLISM IN THALASSEMIA MAJORValidity of the Assumption of Tracer Equilibrium with respect to the Excretion of Vitamin B 12Liver B12 in Subjects With and Without Vitamin B12 Deficiency. A Quantitative and Qualitative StudyFailure of Long-Term Oral Vitamin B12 Therapy to Replenish Body B12 StoresAssessment of Body Stores of Vitamin B12 During Maintenance Therapy of Pernicious Anaemia with Depot Cyanocobalamin Preparation (Betolvex®)The vitamin B12 content of human liver tissue obtained by aspiration biopsyObservations on the Metabolism of Folic Acid in Pernicious AnaemiaVitamin B12 Content of Liver and Serum in Malignant NeoplasiaHepatic and Serum Vitamin B12 Content in Liver DiseaseCalculations on vitamin B 12 turnover in man with a note on the maintenance treatment in pernicious anemia and the radiation dose received by patients ingesting radiovitamin B 12The Effect of Incubation of Plasma with Russell's Viper Venom on the Recalcification Clotting Time 1 December 1958Volume 49, Issue 6Page: 1361-1367KeywordsAnemiaBiopsyHospital medicineInflammationLactobacillusLiverMedical servicesPernicious anemia ePublished: 1 December 2008 Issue Published: 1 December 1958 PDF downloadLoading ...

Absorption of radioactive vitamin B12 in nonanemic patients with combined-system disease.

BEFORE the advent of liver or vitamin B12 treatment of pernicious anemia, approximately 80 per cent of patients who died of this disease had neurologic signs resulting from demyelinization of the posterior and lateral columns of the spinal cord.1 This neurologic disorder has been variously termed combined-system disease, posterolateral sclerosis, subacute combined degeneration of the spinal cord and funicular degeneration. Its pathology, clinical course and response to treatment are well kno-n.2 3 4 5 6 7 In vitamin B12 deficiency, anemia usually precedes the development of neurologic signs and symptoms, but occasionally this sequence is reversed and extensive neurologic damage may occur in . . .

Liver storage of vitamin B12 by the rat.

SummaryThe storage of vit. B12 in rat liver was investigated after feeding rats graded levels of crystalline vit. B12 or of crude supplements. The vit. B12M potency was determined with L. leichmannii after short-term auto-claving of the liver homogenates at pH 7.0. The vit. B12 potency was shown to increase as the level fed was increased. The values were only 1/10 to 1/20 those for beef liver, however, and the vit. B12 potency of rat liver was not reduced appreciably by alkali treatment. Studies on the desoxyribonucleic acid levels in rat livers and studies with Euglena gracilis assays for vit. B12 indicated that the vit. B12 activity remaining, as determined with L. leichmannii after alkali treatment, may be due to the presence of alkali stable forms of vit. B12 as well as to desoxyribosides. The liver weight was shown to be greater per unit of body weight for the rats fed the vit. B12-deficient diet as compared to the optimum diet.