Liver ADH Research Articles

Effect of ethanol on growth and liver components in mice

The growth rate and body weight of male mice of the C 57Bl/1OJ strain maintained on 15 per cent ethanol solution for periods of one to 12 months was comparable to that of the controls. There was no difference in the liver nitrogen content. However, the liver ADH content of the ethanol groups was significantly higher and the liver glycogen content was significantly lower than that of the controls. Mice paired-fed with the ethanol groups and provided with an additional 300 mg. of sucrose daily grew at a subnormal rate and attained subnormal body and liver weights. The liver nitrogen and liver ADH level was significantly lower than that of the ethanol groups. However, the liver glycogen content of the paired-fed mice was significantly higher than that of the ethanol groups and comparable to that of the control groups. Ethanol calories can be utilized for growth, maintenance of body weight and maintenance of the nitrogen integrity of the liver. The increase in liver ADH content and the pronounced decrease in liver glycogen content are a result of ethanol, per se, rather than a result of reduction in food intake.

Effect of prolonged ethanol intake on body weight, liver weight and liver nitrogen, glycogen, ADH, NAD and NADH of mice

Effect of prolonged ethanol intake on body weight, liver weight and liver nitrogen, glycogen, ADH, NAD and NADH of mice

Dissociation constants of the liver alcohol dehydrogenase coenzyme complexes

1. 1. The fluorescence increase occurring when DPNH is bound to liver ADH has been used for determining the dissociation constant of ADH. DPNH at pH values from 5.3 to 10. The effects of formate and chloride were studied. 2. 2. The dissociation constant of ADH.DPN was determined under corresponding conditions, using as indicator the competition of DPNH and DPN for the same binding site(s). D ox, unlike D red, ascribes a univalent dissociation curve with p K′ = 7.5. The implications of this are discussed. 3. 3. Whereas good agreement was found between the present values for D red and those calculated from previous kinetic measurements, discrepancies were observed in the case of D ox. Since formate competes with ethanol for a binding site at ADH.DPN, these discrepancies can be ascribed to the formation of a ternary ADH.DPN.ethanol complex, interfering with the kinetics particularly in acid solution.