Live Rabbits Research Articles

Hydrogel-based fluorescence assay kit for simultaneous determination of ceftazidime and avibactam

Monitoring the concentration of antibiotics rapidly and cost-effectively is crucial for accurate clinical medication and timely identification of drug-induced illnesses. Here, we constructed a novel fluorescent assay kit to monitor Zavicefta, an effective antibiotic composed of avibactam (AVI) and ceftazidime (CFZ) to treat carbapenem-resistant gram-negative bacteria infections. AVI can emit fluorescence, but CFZ cannot. To enable simultaneous measurement of both in one kit, we designed molecularly imprinted polymer (MIP) modified quantum dots (QDs) for CFZ determination. MIPs have received significant attention as an artificial antibody due to their exceptional specificity for various targets, particularly drugs with small molecular weight. Under the excitation wavelength of 350 nm, the detection process involves a decrease in QDs’ fluorescence signal at 600 nm owing to the “gate effect” between MIP and CFZ and the internal filtration effect between CFZ and QDs. Simultaneously, a fluorescence emission characteristic peak at 420 nm for AVI emerges. In addition, to simplify the operation procedure and improve determination throughput, the detection agents were incorporated into a hydrogel and placed in a 96-well plate, enabling concurrent quantification of AVI and CFZ within the respective range of 80–1000 μM and 1–1000 μM. The developed assay kit successfully determined AVI and CFZ in human serums and therapeutic drug monitoring in a live rabbit model. Recoveries of AVI and CFZ were 92.7–114%, with relative standard deviations below 6.0%. Moreover, a smartphone was employed to read the fluorescence signals, which was beneficial for cost reduction and out-of-lab analysis. This study will deliver a pragmatic resolution to developing high-throughput assay kits for drug determination.Graphical

Prevalence of Passalurus ambiguus in domestic rabbits (Oryctolagus cuniculus) in Karbala province, Iraq

Commercial rabbit farming is primarily focused on the production of rabbit meat, although the presence of gastrointestinal disorders can impede their productivity. Endoparasites significantly contribute to the development of several diseases in rabbits, leading to higher rates of illness and death. Passalurus ambiguus is a widely distributed nematode that commonly infects the intestines of rabbits and hares. Parasitic disease can have a substantial economic impact on the industry by reducing the commercial value of rabbit meat and skins, impeding growth and development, and leading to mortality. This study aims to examine the frequency of gastrointestinal parasites in domestic rabbits that are bred in Karbala province, Iraq. Throughout the trial, fecal samples were collected from a randomly selected group of 48 live rabbits. The feces are collected directly from the anus of each living animal. The samples were tested and the presence of eggs and nematodes was detected in the infected samples. Analysis of samples was carried out at the Parasitology Laboratory, college of Veterinary Medicine, Kerbala university, using the flotation technique. Feces from 48 rabbits (ages 1-2) years. The feces are taken out of each animal directly from the anus. Upon testing, it was found that 16 out of 48 fecal samples, accounting for a prevalence of 33.33 %, were infected with at least one nematode. Chi-square (χ2) statistics. Age and genders are not statistically significant at P values ≥ 0.05. This study identified the presence of Passalurus ambiguus in rabbits of varying ages residing in Karbala region, Iraq, including both male and female domestic rabbits. Determining the age and gender of rabbits affected by passalurosis would enable the management of the infection level in these animals. Regular use of a consistent and dependable diagnostic method is essential in industrial rabbit farms to detect and monitor gastrointestinal parasite illnesses. Gastrointestinal parasites continue to be significant health issues that lead to the mortality of rabbit litters in production. Stress and a reduction in immune response may be crucial factors driving this phenomenon.

The Use of Rabbits Used to Propagate Human Lice for Research.

The globally important human diseases of trench fever, epidemic typhus, and relapsing fever are vectored by the human louse Pediculus humanus humanus. Although these conditions are epidemically quiescent at present, they persist in socially dysfunctional situations of war, deprivation, and crowding. The taxonomically closely related head louse, Pediculus humanus capitis, does not respect economic or social status and is quite common in most countries. The 2 types of lice are now recognized as conspecific ecotypes of a single species. While the body louse has been adapted for propagation in the laboratory by feeding in vivo on live rabbits, a similar animal model has not been developed for the host-specific head louse. Accordingly, research for treatment and control of the head louse has largely been performed by using laboratory-reared body lice. This review describes methods for the propagation of body lice in the laboratory and outlines at least 4 areas of research that require sufficient numbers of aged body louse cohorts produced in rabbits for use in controlled studies: 1) pediculicide development and resistance, 2) immunity and vaccine potential, 3) endosymbiotic bacteria needed by lice for nutrition, and 4) lice as vectors of human disease. The review concludes with a discussion of several ethical issues involved with the standard method of using unsedated rabbits and recommends consideration of providing sedating anesthetics for rabbits used in louse feeding procedures.

Multichannel microneedle dry electrode patches for minimally invasive transdermal recording of electrophysiological signals

The collection of multiple-channel electrophysiological signals enables a comprehensive understanding of the spatial distribution and temporal features of electrophysiological activities. This approach can help to distinguish the traits and patterns of different ailments to enhance diagnostic accuracy. Microneedle array electrodes, which can penetrate skin without pain, can lessen the impedance between the electrodes and skin; however, current microneedle methods are limited to single channels and cannot achieve multichannel collection in small areas. Here, a multichannel (32 channels) microneedle dry electrode patch device was developed via a dimensionality reduction fabrication and integration approach and supported by a self-developed circuit system to record weak electrophysiological signals, including electroencephalography (EEG), electrocardiogram (ECG), and electromyography (EMG) signals. The microneedles reduced the electrode–skin contact impedance by penetrating the nonconducting stratum corneum in a painless way. The multichannel microneedle array (MMA) enabled painless transdermal recording of multichannel electrophysiological signals from the subcutaneous space, with high temporal and spatial resolution, reaching the level of a single microneedle in terms of signal precision. The MMA demonstrated the detection of the spatial distribution of ECG, EMG and EEG signals in live rabbit models, and the microneedle electrode (MNE) achieved better signal quality in the transcutaneous detection of EEG signals than did the conventional flat dry electrode array. This work offers a promising opportunity to develop advanced tools for neural interface technology and electrophysiological recording.

Enhancing neonatal thoracoscopic surgical training with rabbit model

BackgroundThoracoscopy, which has an increasing role in the treatment of indexed neonatal surgical conditions, requires adequate training. To support this, the current study aimed to evaluate the feasibility and effectiveness of using live rabbit models in neonatal thoracoscopic skills training among paediatric surgeons. MethodsFollowing didactic lectures and demonstrations, the participants were given hands-on opportunities to perform thoracoscopic procedures. The feasibility and effectiveness of using live rabbit models in neonatal thoracoscopic skills training among paediatric surgeons were evaluated with pre-/post-course procedural confidence scores and a questionnaire. ResultsThis study included 13 paediatric surgeons—2 (15 %) males and 11 (85 %) females—who were evenly distributed. There were four basic surgical trainees, five higher surgical trainees and four fellows in paediatric surgery (mean surgical practice experience: 4.5 ± 3.7 years). Most had experience assisting paediatric (70 %) and neonatal (62 %) thoracoscopic surgery. Only 30 % had experience as the chief surgeon of paediatric thoracoscopic surgery, with none on neonates. Significant improvement was seen in procedural confidence as the assistant and chief surgeon of all procedures post-workshop. The surgeons rated the model positively. ConclusionThe procedural confidence level of paediatric surgeons improved significantly after workshop participation. This realistic and easily reproducible model can help perfect thoracoscopic skills. Therefore, its integration into paediatric surgical training would promote surgical skill proficiency and could improve surgeons’ confidence in neonate operations.

Economic analysis of banana peel and sweet potato vines in diets for rabbits.

This study aimed to evaluate the costs of using banana peel (BP) and sweet potato vine (SPV) in rabbit diets. BP and SPV were chosen to replace maize and alfalfa hay, because, in addition to the ingredients having similar nutritional characteristics, they are among the most expensive ingredients in rabbit diets. Data were obtained through a biological assay carried out in the Cuniculture Laboratory of the Universidade Federal de Santa Maria, Santa Maria, RS, Brazil. Fifty New Zealand white rabbits, weaned at 35days of age, were fed during the growth phase (35 to 84days) with increasing levels of BP and SPV, replacing maize and alfalfa hay (T0, T25, T50, T75 and T100% replacement). A diet containing 100% replacement (T100) had the lowest cost per kilogram, which was R$ 1.18/kg, while the controlled diet was 57% more expensive, costing R$ 2.08/kg. The total operating cost to produce a rabbit with control treatment was R$10.93/head and at T100, it was R$6.51/head. The animal income for a live rabbit was R$ 24.08 and R$ 23.95 in treatments T0 and T100, respectively. Regarding the gain margin per animal (GMA), in TO it was R$ 13.16 while in T100 it was R$ 17.44, therefore the GMA was 32.55% higher when using the T100 diet. In conclusion, it is more economical and feasible to feed rabbits with diets containing 100% BP and SPV, replacing maize and alfalfa hay.

Enhanced Interstitial Fluid Extraction and Rapid Analysis via Vacuum Tube-Integrated Microneedle Array Device.

Advancing the development of point-of-care testing (POCT) sensors that utilize interstitial fluid (ISF) presents considerable obstacles in terms of rapid sampling and analysis. Herein, an innovative strategy is introduced that involves the use of a 3D-printed, hollow microneedle array patch (MAP), in tandem with a vacuum tube (VT) connected through a hose, to improve ISF extraction efficiency and facilitate expedited analysis. The employment of negative pressure by the VT allows the MAP device to effectively gather ≈18µL of ISF from the dermis of a live rabbit ear within a concise period of 5min. This methodology enables the immediate and minimally invasive measurement of glucose levels within the body, employing personal healthcare meters for quantification. The fusion of the VT and MAP technologies provides for their effortless integration into a comprehensive and mobile system for ISF analysis, accomplished by preloading the hose with custom sensing papers designed to detect specific analytes. Moreover, the design and functionality of this integrated VT-MAP system are intuitively user-friendly, eliminating the requirement for specialized medical expertise. This feature enhances its potential to make a significant impact on the field of decentralized personal healthcare.

Development of a Technique Using Artificial Membrane for In Vitro Rearing of Body Lice Pediculus humanus humanus.

Human lice are the only hematophagous ectoparasites specific to human hosts. They transmit epidemic typhus, trench fever and relapsing fever, diseases which have already caused millions of deaths worldwide. In order to further investigate lice vectorial capacities, laboratory-controlled live lice colonies are essential. Previously developed lice-rearing methods significantly advanced research on louse-borne diseases and louse biology. In this study, we aimed to develop a rearing technique for the Orlando (Or) strain of body lice on an artificial membrane. We tested two systems, namely the Hemotek feeding system and a Petri dish with the lice being fed through a Parafilm membrane. Lice longevity and development were drastically affected by the blood anticoagulant. Additionally, heparinised human blood on a Petri dish was the best candidate when compared to the control group (reared on a rabbit). Therefore, this strategy was applied to 500 lice. Development into adulthood was recorded after 21 days (17 days for the rabbits), and 52 eggs were deposited (240 for the rabbits). In this study, we were able to maintain one generation of body lice on an artificial membrane with comparable feeding and longevity rates to those fed on live rabbits. However, lice fecundity decreased on the artificial membrane. In vitro lice-rearing experiments will enable pathogen infection assays and pesticide bioassays to be carried out in accordance with animal welfare requirements.

A perioperative layered autologous tissue expansion graft for hollow organ repair

Tissue engineering has not been widely adopted in clinical settings for several reasons, including technical challenges, high costs, and regulatory complexity. Here, we introduce the Perioperative Layered Autologous Tissue Expansion graft (PLATE graft), a composite biomaterial and collagen-reinforced construct with autologous epithelium on one side and smooth muscle tissue on the other. Designed to mimic the structure and function of natural hollow organs, the PLATE graft is unique in that it can be produced in a standard operating theatre and is cost-effective. In this proof-of-principle study, we test its regenerative performance in eight different organs, present biomechanical and permeability tests, and finally explore its in vivo performance in live rabbits.

Tissue-targeted and localized AAV5-DCN and AAV5-PEDF combination gene therapy abrogates corneal fibrosis and concurrent neovascularization in rabbit eyes in vivo

PurposeCorneal fibrosis and neovascularization (CNV) after ocular trauma impairs vision. This study tested therapeutic potential of tissue-targeted adeno-associated virus5 (AAV5) mediated decorin (DCN) and pigment epithelium-derived factor (PEDF) combination genes in vivo. MethodsCorneal fibrosis and CNV were induced in New Zealand White rabbits via chemical trauma. Gene therapy in stroma was delivered 30-min after chemical-trauma via topical AAV5-DCN and AAV5-PEDF application using a cloning cylinder. Clinical eye examinations and multimodal imaging in live rabbits were performed periodically and corneal tissues were collected 9-day and 15-day post euthanasia. Histological, cellular, and molecular and apoptosis assays were used for efficacy, tolerability, and mechanistic studies. ResultsThe AAV5-DCN and AAV5-PEDF combination gene therapy significantly reduced corneal fibrosis (p < 0.01 or p < 0.001) and CNV (p < 0.001) in therapy-given (chemical-trauma and AAV5-DCN + AAV5-PEDF) rabbit eyes compared to the no-therapy given eyes (chemical-trauma and AAV5-naked vector). Histopathological analyses demonstrated significantly reduced fibrotic α-smooth muscle actin and endothelial lectin expression in therapy-given corneas compared to no-therapy corneas on day-9 (p < 0.001) and day-15 (p < 0.001). Further, therapy-given corneas showed significantly increased Fas-ligand mRNA levels (p < 0.001) and apoptotic cell death in neovessels (p < 0.001) compared to no-therapy corneas. AAV5 delivered 2.69 × 107 copies of DCN and 2.31 × 107 copies of PEDF genes per μg of DNA. AAV5 vector and delivered DCN and PEDF genes found tolerable to the rabbit eyes and caused no significant toxicity to the cornea. ConclusionThe combination AAV5-DCN and AAV5-PEDF topical gene therapy effectively reduces corneal fibrosis and CNV with high tolerability in vivo in rabbits. Additional studies are warranted.

(225) Rabbits Provide a Viable Model for Preclinical Penile Implant Studies

Abstract Introduction Inflatable penile implants (IPP) are the mainstay treatment for refractory erectile dysfunction (ED), but device infection is common and confers significant morbidity. Besides the addition of antibiotic and hydrophilic coatings, improvements targeting this complication are lacking. Preclinical in vivo studies to study methods of decreasing biofilm and mitigating infection sequelae are hindered by the lack of animal models containing functional IPPs. Previously, IPP fragments have been subcutaneously placed to study antimicrobial strategies, but the placement of complete, functioning IPP cylinders has not been demonstrated. Objective This biocompatibility study aimed to demonstrate the feasibility of placing and inflating IPPs in rabbits, with the goal of developing a viable model to study penile implant infections. Methods This study utilized 2 cadaveric and 2 live male New Zealand White rabbits. Experimental protocols were approved by IACUC (21-08-418). Cadaveric rabbits were utilized to evaluate surgical technique and ultrasound parameters prior to in vivo study. Following anesthesia and surgical site preparation, functioning IPPs were placed into the rabbits’ flanks. Upon dissection down to the plane between the panniculus carnosus and underlying muscle fascia, a subcutaneous pouch was developed to accommodate the implant. Following implantation, unrestricted ambulation was permitted, and the rabbits were observed daily for activity and recovery. Wounds were inspected daily for drainage, erythema, warmth, and swelling. Inflation-deflation cycling was performed following a 3-day post-implantation recovery period and evaluated via ultrasound. Surveillance was conducted for 14 days, after which the animals were euthanized. Results A single cylinder 14 cm implant without rear tip extender was successfully placed into each rabbit. IPP cycling mimicking the inflation protocol was successfully completed without tissue damage or harm to the animals. All IPP components were successfully visualized via ultrasound, which demonstrated the superficial position of the implant (Figure 1). Over a 14-day observation period, no concerns were identified, and signals of pain or distress, including gait disturbance, hypoactivity, restlessness, weight loss, dehydration, or reduced eating/drinking, were not observed, indicating the biocompatibility of functioning penile implants in the back of rabbits. Conclusions Preclinical in vivo models are the primary representative way to investigate IPP infection. This rabbit model may serve as an effective tool for the evaluation of novel infection prevention strategies following penile prosthesis implantation for ED. Disclosure Any of the authors act as a consultant, employee or shareholder of an industry for: PHC: Boston Scientific and Coloplast.

Use of dehydrated sainfoin in rabbit feeding. Effects of a moderate dietary incorporation on performance and health of does and growing rabbits under an optimal farming environment

The effects of a moderate incorporation of dehydrated sainfoin Perly cultivar (DS) in rabbit feeds on the performance and health of reproductive and growing rabbits were analysed over two consecutive reproductive cycles in a professional breeding environment. Two groups of 192 does and associated litters were fed isonutritive feeds containing either 0 or 13% dehydrated sainfoin (respectively C (control) vs. S (“sainfoin”) groups) in replacement mainly of dehydrated alfalfa. Growing rabbit feeds C and S included 0 and 15.6% safoin, respectively. Doe live weight, number of live rabbits at birth and stillborn rate were not affected by dietary DS incorporation. In cycle 1, fertility rate was 10% higher for the S-group, but was similar among the groups in the 2nd cycle (significant interaction). Incorporation of DS had no impact on kit growth before weaning, but improved the post-weaning growth rate by 6% (P&lt;0.001) and the feed conversion ratio by 7%. Dietary DS incorporation had no effect on doe mortality, which was very low (&lt;2%, P=0.07). Doe culling was half lower with sainfoin incorporation in cycle 1 (25% in group C vs. 12% in group S; P&lt;0.05). In cycle 2, doe culling rate was low (3.2%) and similar among diets (significant interaction between diet and cycle effects). Pre-weaning mortality of kits was low and slightly higher for S-group (1.1 vs. 1.5%). Post-weaning mortality was also low and was reduced with sainfoin dietary incorporation (3.0 vs. 1.8%; P&lt;0.001). A moderate incorporation of dehydrated sainfoin can be recommended for growing rabbits and for reproducing does feeds.

A rabbit model of tracheal collapse for optimal self-expanding metal stents.

Investigating the characteristics of tracheas can help the understanding of diseases related to the trachea, particularly tracheal collapse (TC) in dogs. This study aimed to compare the mechanical properties of tracheas from New Zealand White (NZW) rabbits and dogs and to introduce a method for inducing a model of TC in the normal trachea. Tracheal samples were obtained from NZW rabbit cadavers (n=5) weighing 3.62-3.92 kg and from dog cadavers (n=5) weighing 2.97-3.28 kg. Three live NZW rabbits weighing 3.5-4.0 kg were used to establish the model. The radial forces of both sample sets were measured using a digital force gauge and statistically compared. Subsequently, TC was surgically induced in three female NZW rabbits by physically weakening their tracheal cartilage under general anesthesia. Their clinical signs were monitored for 3 months, and radiographic examinations were performed monthly for 3 months. The mean radial forces of the two sample sets were comparable (P>0.05). The clinical signs, radiographic examinations, and macroscopic examinations were all comparable to those of dogs with TC. The cadaveric study between the rabbits and dogs demonstrated that the surgically induced rabbit model of TC is an excellent candidate for the experimental study of dogs with TC. This study also provides a reference of tracheal radial force values to enable selection of appropriate mesh types and wire diameters of self-expanding metal stents.

How to do it: Teaching surgical skills to medical undergraduates.

Medical students must be capable of performing clinical and surgical procedures in outpatient care and initial emergency care in all stages of the biological cycle. Here, we describe the surgical skills schedule with different animal models fulfilled at the Municipal University of São Caetano do Sul (USCS) Medical School, São Caetano do Sul, SP, Brazil, during the surgical abilities module.We retrospectively reviewed the surgical abilities module schedule provided at the USCS Medical School from 2015 until 2020; in this paper, we describe the use of different animal models. The activities were developed for two semesters during medical school and included an ox tongue, cylindrical Styrofoam, chicken leg and neck, live rabbits, and pigs.Practical surgical teaching starts with sutures using the ox tongue, after which students are taught to perform tenorrhaphy using cylindrical Styrofoam and chicken legs, followed by vascular anastomosis using the chicken trachea and esophagus. Rabbits are appropriate for training a variety of procedures such as cystostomy, gastrostomy, and appendectomy. Pigs allow for the simulation of several types of procedures such as chest drainage.Surgical training for medical undergraduates was demonstrated with an evolutionary intent, starting with simple sutures and ending up with basic emergency room surgical procedures.

Time-dependent in situ structural and cellular aberrations in rabbit cornea in vivo after mustard gas exposure

An array of corneal pathologies collectively called mustard gas keratopathy (MGK) resulting from ocular exposure to sulfur mustard (SM) gas are the most prevalent chemical warfare injury. MGK involves chronic ocular discomfort that results in vision impairment. The etiology of MGK remains unclear and poorly understood primarily due to a lack of scientific data regarding structural and cellular changes in different layers of the cornea altered by mustard vapor exposure in vivo. The goals of this study were to (a) characterize time-dependent changes in different layers of corneal epithelium, stroma, and endothelium in live animals in situ by employing state-of-the-art multimodal clinical ophthalmic imaging techniques and (b) determine if SM-induced acute changes in corneal cells could be rescued by a topical eye drop (TED) treatment using in an established rabbit in vivo model. Forty-five New Zealand White Rabbit eyes were divided into four groups (Naïve, TED, SM, and SM + TED). Only one eye was exposed to SM (200 mg-min/m3 for 8 min), and each group had three time points with six eyes each (Table-1). TED was topically applied twice a day for seven days. Clinical eye examinations and imaging were performed in live rabbits with stereo, Slit-lamp, HRT-RCM3, and Spectralis microscopy system. Fantes grading, fluorescein staining, Schirmer's tests, and applanation tonometry were conducted to measure corneal haze, ocular surface aberrations, tears, and intraocular pressure respectively. H&E and PSR staining were used for histopathological cellular changes in the cornea. In vivo confocal and OCT imaging revealed significant changes in structural and morphological appearance of corneal epithelium, stroma, and endothelium in vivo in SM-exposed rabbit corneas in a time-dependent manner compared to naïve cornea. Also, SM-exposed eyes showed loss of corneal transparency characterized by increased stromal thickness and light-scattering myofibroblasts or activated keratocytes, representing haze formation in the cornea. Neither naive nor TED-alone treated eyes showed any structural, cellular, and functional abnormalities. Topical TED treatment significantly reduced SM-induced abnormalities in primary corneal layers. We conclude that structural and cellular changes in primary corneal layers are early pathological events contributing to MGK in vivo, and efficient targeting of them with suitable agents has the potential to mitigate SM ocular injury.

Rabbits Divergently Selected for Total Body Fat Content: Changes in Proximate Composition and Fatty Acids of Different Meat Portions.

Simple SummaryThe purpose of the investigation was to determine the effect of the selection for the total fat content of the rabbit body on the fat content and fatty acid composition of different meat cuts. The higher the fat content of the given meat, the more effective the selection was. Authors found that the meat of fatter rabbits is healthier, which can be used in the production of baby food.The present research studied the potential of a four-generation divergent selection (Pannon maternal rabbit lines) based on the total body fat content to create two rabbit lines with different meat attributes: a Fat line to deliver greater amounts of healthy fatty acids for newborns and infants, and a Lean line intended to provide lean meat for everyday consumption. Selection was based on the fat index calculated in 10-week-old live rabbits by means of computed tomography (CT). For each generation, 60 rabbits/line were fed ad libitum with commercial pellet from weaning (5 weeks) to slaughter (11 weeks). A total of 15 rabbits/line were randomly selected for meat quality evaluations: the longissimus thoracis et lumborum muscles (LTLs), hind legs (HLs), forelegs (FLs) and abdominal wall (AW) were analyzed for their proximate composition and fatty acid (FA) profile. FA contents were also calculated. Results highlighted that it was possible to obtain leaner meat for everyday consumption in most meat portions starting from generation 4 (7.93 vs. 11.9, 5.10 vs. 5.98 and 7.26 vs. 10.9 g of lipids/100 g of meat in Lean and Fat groups for the FLs, HLs and AW, respectively). The sole exception was the LTLs, which were not affected by the divergent selection. The total PUFA amount increased in FL and AW (p < 0.05) portions of the Fat line only, attributable to a greater n-3 amount (151 vs. 216 and 73 vs. 143 mg/100 g of meat in Lean and Fat groups for the FLs and AW, respectively).

A new deep eutectic solvent-agarose gel with hydroxylated fullerene as electrical “switch” system for drug release

A novel portable conductive system of hydroxylated fullerene (TEGs-C60) embedded on the deep eutectic solvent (DES, choline chloride/malic acid)-agarose gel was developed for on-demand curation with skin contact. It has been found with better elasticity, conductivity and thermal stability than agarose hydrogel. Especially, the electro-responsive drug releasing amount on the skin of mice and live rabbits through small batteries was proved to be 4–5 times of those without electrical stimulation, which reached the level of clinic daily dosage. Meanwhile, it also showed good biocompatibility and much higher antibacterial rate against S. aureus (78.67 ± 0.80 %) together with E. coli (92.08 ± 1.50 %) than those of commercial chitosan gel. The new electrical “switch” gel system provided an effective and useful development strategy for advanced drug delivery.

Comparison of Healing Effect Between Fibrin Gel Complex Bone Morphological Protein and Reconstituted Bone Xenograft After Reconstruction of Sports Ligament Injury

To study the effect of fibrin gel complex bone morphological protein (FG-BMP) and RBX on the healing of tendon-bone interface. 51 live rabbits were selected, and 51 rabbits were randomly divided into 3 groups: FG-BMP, RBX and control groups. Bilateral ACL reconstruction was performed in 51 rabbits, with different biomaterials injected at the tendon-bone interface, and none applied in the FG-BMP, RBX, and control groups. Material extraction, imaging examination, histological analysis, and biomechanical tests were conducted at 2 weeks after surgery, 6 weeks later, and 12 weeks after surgery, respectively. The tendon-interbone growth rate of rabbits using FG-BMP biomaterials and RBX biomaterials was significantly improved in the FG-BMP and RBX groups compared with the control group (P &lt; 0.05). Histological results showed that all rabbits in the RBX group were surrounded by newborn bone tissue around the tendon-bone transplantation tendon and had a significantly greater number of newborn bone tissue than in the FG-BMP group (P &lt; 0.05). The biomechanical test results showed that the two groups of FG-BMP biomaterials improved the maximum tension compared with the control group (P &lt; 0.05), where the RBX tension, the difference was significant (P &lt; 0.05), and the FG-BMP group although higher than the control group (P &gt; 0.05). Conclusion: In the comparison of promoting tendon-bone interface healing, FG-BMP biomaterials and RBX biomaterials have significant results, especially RBX materials have obvious advantages in accelerating tendon-bone interface healing.

Feed incorporation of dehydrated sainfoin: effects on health and performances of does and growing rabbits

The performance and health of does and growing rabbits were compared over three consecutive reproductive cycles for three groups of 20 nulliparous does and their litters (DS0, DS13, DS26) fed isonutritive feeds containing 0, 13 or 26% dehydrated sainfoin (DS, Perly cultivar). Feed intake, live weight and fertility of does were not affected by DS feed incorporation. The number of live kits at birth increased linearly with increasing DS incorporation (+1.5 from DS0 to DS26, P=0.042) and the stillborn rate tended to linearly decrease in groups fed DS (16.6 vs. 10.4%, P=0.086). Increasing the level of DS in feeds had no impact on the growth of the kits before weaning, but led to a linear reduction in the post-weaning growth rate (P&lt;0.01, –2 for 26% DS), whereas the feed conversion ratio increased linearly with DS incorporation (P&lt;0.01, 2.91 vs. 2.98, resp. for DS0 and DS26). No effect of DS feed incorporation was detectable on doe and kit mortality rates. Excretion of coccidia by both does and growing rabbits was not affected by DS incorporation. For 70 d old rabbits, the levels of immunoglobulins A and G and of white blood cells were not significantly different between groups and high levels of IgG (average: 8.1 mg/mL) were recorded, suggesting a coccidia infestation. Overall doe mortality remained under 5% and was not affected by the reproductive cycle (P=0.24). The stillborn rate decreased from 18 to 6%, (P&lt;0.01) from cycle 1 to 2, and the number of live rabbits at birth increased from 8.0 to 10.7 (P&lt;0.01). Kit mortality remained low before weaning (under 2.5%), and very low after weaning (&lt;1%). Excretion of coccidia by does decreased from cycle 1 to cycle 3, whereas excretions by growing rabbits remained stable.

A Rare Cause of Fever in a Term Neonate.

A Rare Cause of Fever in a Term Neonate.