In six forest parks of Moscow and four rural forests (5 plots each, n = 50), soil physical, chemical and microbial properties of the upper 10 cm layer were assessed in combination to vegetation properties. The content of carbon (C), nitrogen (N), and phosphorus (P) in soil and microbial biomass was determined. It was revealed that soil density, pH value, content of N–\({\text{NO}}_{3}^{ - },\) Ca and heavy metals (Pb, Cu, Ni, Zn) increase in forest parks compared to rural forests. In the soil of the forest parks, a decrease in the content of microbial biomass C (Cmic), its basal respiration (BR), and microbial C- and N-availability (Cmic/C, Nmic/N, BR/C) was noted. The changes of soil microbial properties are mainly driven by the abundance of leaf litter and the content of available soil C (13–35% of the explained variance). The microbial response to the soil enrichment by low molecular weight organic substrates (carbohydrates, carboxylic and phenolic acids, amino acids, amino sugars) in forest parks and rural forests did not differ significantly. In the soils of forest parks, no changes in microbial mineralization and immobilization of P (Pmic, Pmic/P) were found as well. The impact of urbanization on the forest ecosystems has led mainly to a decrease in the intensity of processes associated with soil C and N cycles. Apparently, such changes are caused by the recreational activity and the management practice of green spaces in the city, which leads, in particular, to a decrease in the amount of forest litter in parks compared to rural forests.
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