We present the first annotated checklist of the subfamily Prioninae Latreille, 1802 (Coleoptera, Ceram-bycidae) in Lithuania. At least 140 published and unpublished records of Prioninae were analyzed and collected specimens were verified. As a result, one species, Aegosoma scabricorne (Scopoli, 1763), was found to be new for Lithuanian fauna and three species, Ergates faber (Linnaeus, 1761), Tragosoma depsarium (Linnaeus, 1767), and Prionus coriarius (Linnaeus, 1758), were confirmed in the country. The information on the local occurrence, natu-ral history, general distribution, and conservation status of these species is reviewed.