Generally, pressure, temperature, depth of organic matter/kerogen burial and organic and inorganic matter interactions can influence the hydrocarbon generation process. However, different understandings were proposed to interpret the role of pressure in hydrocarbon generation. Meanwhile, previous investigations of the influence of pressure on hydrocarbons generation are mainly focused on fluid pressure (FP), only a few work was involved in the effect of lithostatic stress (LS) due to the limitation of experimental techniques. In this study, simulation experiments were conducted to investigate the influence of pressure, including lithostatic stress (LS) and fluid pressure (FP) on yield and process of hydrocarbon generation by a modified simulation instrument. Two systematic comparative pyrolysis experiments, including pressured experiments with LP range from 27.6 to 179.4 MPa and FP range from 12.0 to 109.2 MPa, and non-pressured experiments with LP for 6.0 MPa and FP range from 2.0 to 5.0 MPa were conducted in hydrous experimental condition. The results show that the peak yields of the expelled oil and the liquid hydrocarbons in pressured experiments and non-pressured experiments are 42.48 mg/g·TOC, 52.00 mg/g·TOC, 70.14 mg/g·TOC, and 72.15 mg/g·TOC, respectively. And Ro, ratio of the expelled oil to eluted oil, the accumulated yield of the expelled oil and liquid hydrocarbons, are lower in pressured experiments than that of non-pressured experiments. However, the accumulated yields of eluted oil and residual oil are higher in pressured experiments than that of non-pressured experiments. Additionally, the expulsion efficiencies of the oil range from 20% to 25%, which are lower in pressured experiments than that of non-pressured experiments near the oil generation-window. In addition, the yields of hydrocarbon gas in pressured experiments are greater compared to the yields in non-pressured experiments at the high maturity stage. Based on the experimental results, hydrocarbon generation process can be divided into three stages. Generally, LS and FP could retard the process of mudstone maturation as well as the generation and expulsion of the liquid hydrocarbons. Hence, the residual liquid hydrocarbons in high-pressure condition can act as another source of natural gas generation in the high maturity stage. The results could help us to properly evaluate hydrocarbon generation potentials of sources rocks at petroleum-producing sedimentary basins in China and elsewhere.