Color Of Liquor Research Articles

Investigation of the quality of Lu'an Guapian tea during Grain Rain period by sensory evaluation, objective quantitative indexes and metabolomics

The harvest date is a crucial factor in determining tea quality. For Lu'an Guapian (LAGP) tea, Grain Rain period (GRP) represents a pivotal phase in the transformation of tea quality. The sensory evaluation, computer vision and E-tongue revealed that the liquor color score, B and G values of tea infusion were increased during GRP, while the astringency, bitterness intensities and the R value of the tea infusion were decreased. Consequently, the tea infusion exhibited a greener hue and the taste became appropriate during GRP. Non-targeted metabolomics revealed that the majority of amino acids and derivatives was reduced during GRP. Furthermore, flavonoids, in particular flavonol glycosides, exhibited considerable variation during GRP. Finally, nine metabolites were identified as markers for quality transformation during GRP by PLS and Random Forest. This study investigated the quality of LAGP teas during GRP and filled the gap in the variation of LAGP tea quality during GRP.

The quality difference in five oolong tea accessions under different planting management patterns in south Fujian of China

IntroductionOolong tea, celebrated for its significance in Chinese tea culture, was the subject of investigation in this study.MethodsFive varieties of Minnan oolong tea were sampled, each cultivated under two distinct management approaches: conventional management and natural growth methods. The study aimed to discern variations in sensory attributes, encompassing appearance and liquor color, alongside the analysis of chemical composition.Results and discussionThe results indicated that oolong tea cultivated through conventional manual management generally exhibited qualities in terms of shape and foliage appearance, in contrast to those grown naturally. However, naturally grown oolong tea tended to exhibit more favorable aroma and taste profiles compared to conventionally managed counterparts. Furthermore, the content of water extract, amino acids, polyphenols, caffeine, and other pivotal chemical constituents were typically higher in naturally grown tea varieties compared to conventionally managed ones. Conversely, catechin content was found to be more abundant in traditionally managed bushes than in those grown naturally. These findings emphasize the significance of implementing appropriate natural growth management practices to enhance the quality of Minnan oolong tea and maintain ecological sustainability.

Induction of Labour in a District Hospital of Rural Nepal: A Descriptive Cross-sectional Study.

Induction of labour, a medical intervention before spontaneous onset, is employed when the risk of continuing pregnancy is elevated. Common indications include intrauterine growth restriction, preeclampsia, gestational diabetes, placental abnormalities, prelabor rupture of membranes, post-term pregnancy, and intrauterine foetal demise. The objective of this study was to find out the prevalence of induction of labour in a rural setting in Nepal. We conducted a descriptive cross-sectional study in the District Hospital Tehrathum using patients' record files from 14 January 2021 to 14 January 2023. Ethical approval was obtained from Nepal Health Research Council. Demographic variables were collected along with maternal outcomes which include indication of induction of labour, mode of delivery, indication of lower segment caesarean section and foetal outcomes include APGAR score at one and five minutes, birthweight and liquor colour. A total population sampling method was used in the study and 95% confidence Interval was used to calculate the point estimate. Among 640 deliveries during the study period 118 (18.43%) (15.43- 21.43, 95% Confidence Interval) underwent induction of labour. Sixty-three (53.4%) of the 118 patients who underwent induction of labour were primigravida. The prevalence of induction of labour was comparable with previous studies. Neonatal outcome, rate of vaginal and lower segment C-section deliveries after induction of labour using misoprostol is comparable with other studies.

Re-rolling treatment in the fermentation process improves the taste and liquor color qualities of black tea

Fermentation is a vital process occurred under the premise of rolling affecting black tea quality. Theoretically, re-rolling during fermentation will remodel the biochemical conditions of tea leaves, and thus influence black tea quality. Herein, we studied the effect of re-rolling on black tea taste and liquor color. Sensory evaluation showed that re-rolling significantly weakened the astringency taste and improved the redness and luminance of liquor. With re-rolling, the color attributes of a* and L* and the contents of theaflavins and thearubigins were significantly improved. Metabolomics analysis showed that the contents of 110 non-volatile compounds were significantly different among black teas with different rolling treatments. In summary, re-rolling accelerated the oxidation of polyphenols into pigments, the hydrolysis of proteins into amino acids, and the metabolism of alkaloids, organic acids, glycosidically-bound volatiles, and lipids during the fermentation period. Our study provided a novel and simple way to improve black tea quality.

Prevalence of Post Caesarean Wound Infections and Associated Factors among Mothers Delivering from Kampala International University Teaching Hospital

Caesarean section simply is an operative technique by which a fetus is delivered through an abdominal and uterine incisions after a minimum period of 28 weeks of amenorrhea, whilst a post caesarean section wound infection (PCWI) is an infection that occurs after a C- section, usually due to a bacterial infection in the surgical incision site. The purpose of this study was to determine the prevalence of post caesarean wound infections among mothers who delivered from KIU-TH, and to identify common factors associated with post caesarean wound infections among these mothers. Descriptive statistics were used to characterize and highlight the difference among the post-caesarean patients. Data was entered and analyzed using SPSS version 16. The analyzed data was then presented using tables and charts. The results indicate that the prevalence of post-caesarean wound infection at Kampala International University-Teaching Hospital is still low (3.2%) but may increase if the steps are not taken to initiate its prevention and eradication. The results indicate that post-caesarean wound infection is positively and significantly associated with age (X2 = 10.747, P<0.05), the cause/reason of caesarean section (X2 = 62.000, P<0.05), color of liquor (X2 =45.983, P<0.05), duration of labor (X2 =10.067, P< 0.05) and amount of blood lost (X2 = 3.844, P<0.05). The prevalence of PCWIs at KIU-TH was 3.2% and the factors associated were the reason for caesarean section, color of liquor, duration of labor, amount of blood lost. Based on the results and the findings, the study recommends that recognition of the consequences and the building of the strategies to prevent and treat Post-caesarean wound infections at Kampala international university-teaching hospital is essential for reducing post- caesarean maternal morbidity and mortality. In addition, the study also recommends the recognition of the associated risk factors particularly the modifiable and their modification to reduce the occurrence of Post-caesarean wound infection. Keywords: Prevalence, Post Caesarean, Wound Infections and Mothers Delivering

Nontargeted and targeted metabolomics analysis for evaluating the effect of “golden flora” amount on the sensory quality, metabolites, and the alpha-amylase and lipase inhibitory activities of Fu brick tea

To investigate the effects of "golden flora" amount on the sensory quality, metabolites and bioactivities of Fu brick tea (FBT), FBT samples with different "golden flora" amounts were prepared from the same materials by adjusting the water content before pressing. With the increase of "golden flora" in samples, the tea liquor color changed from yellow to orange red and the astringent taste gradually diminished. Targeted analysis demonstrated that (-)-epigallocatechin gallate, (-)-epicatechin gallate, and most amino acids gradually decreased as the increase of "golden flora". Seventy differential metabolites were identified by untargeted analysis. Among them, sixteen compounds including two Fuzhuanins and four EPSFs were positively correlated with "golden flora" amount (P<0.05). The FBT samples with "golden flora" exhibited significantly higher inhibitory potency on α-amylase and lipase than the samples without "golden flora". Our results provide a theoretical basis of guiding FBT processing based on desired sensory quality and metabolites.

The Integration of Metabolomics, Electronic Tongue, and Chromatic Difference Reveals the Correlations between the Critical Compounds and Flavor Characteristics of Two Grades of High-Quality Dianhong Congou Black Tea.

Tea's biochemical compounds and flavor quality vary depending on its grade ranking. Dianhong Congou black tea (DCT) is a unique tea category produced using the large-leaf tea varieties from Yunnan, China. To date, the flavor characteristics and critical components of two grades of high-quality DCT, single-bud-grade DCT (BDCT), and special-grade DCT (SDCT) manufactured mainly with single buds and buds with one leaf, respectively, are far from clear. Herein, comparisons of two grades were performed by the integration of human sensory evaluation, an electronic tongue, chromatic differences, the quantification of major components, and metabolomics. The BDCT possessed a brisk, umami taste and a brighter infusion color, while the SDCT presented a comprehensive taste and redder liquor color. Quantification analysis showed that the levels of total polyphenols, catechins, and theaflavins (TFs) were significantly higher in the BDCT. Fifty-six different key compounds were screened by metabolomics, including catechins, flavone/flavonol glycosides, amino acids, phenolic acids, etc. Correlation analysis revealed that the sensory features of the BDCT and SDCT were attributed to their higher contents of catechins, TFs, theogallin, digalloylglucose, and accumulations of thearubigins (TRs), flavone/flavonol glycosides, and soluble sugars, respectively. This report is the first to focus on the comprehensive evaluation of the biochemical compositions and sensory characteristics of two grades of high-quality DCT, advancing the understanding of DCT from a multi-dimensional perspective.

Urate oxidase treatment increases the quality of autumn Yellowish Yinghong 9 black tea

Microbes can be used to improve the quality of fermented beverages and food. Colletotrichum camelliae is one of the most dominant fungi associated with tea leaves. In the present study, black tea produced from the autumn leaves of Yellowish Yinghong 9 was treated with C. camelliae urate oxidase (CcUOX) to determine whether this treatment could improve tea quality. The effects of recombinant CcUOX on several black tea quality parameters were assessed, such as liquor color, aroma, and levels of theabrownins, thearubigins, total polyphenols, catechins, caffeine, xanthine, and hypoxanthine. CcUOX treatment significantly affected the aroma composition and volatile compound profiles. Specifically, volatiles in the benzenoid/phenylpropanoid, fatty acid metabolism, and amino acid pathways were increased in CcUOX-treated black tea, whereas levels of volatiles in the terpineol and carotenoid pathways were reduced. This suggested that the balance of aroma constituents in Yellowish Yinghong 9 black tea was affected by treatment with CcUOX. The present study provides evidence that pre-treatment of black tea with a single enzyme from a common tea-associated microbe could significantly improve autumn tea quality, validating this method for use in further studies of tea improvement.

Intelligent control for quality improvement in green tea fixation assisted with aroma detection

An intelligent control system is designed in green tea fixation, which can automatically and continuously adjust the fixation parameters for quality improvement. Aroma is detected with PEN-3 electronic nose, and signals are sent to the computer. With the aroma signals, the fixation process is separated into two stages: in the first stage, the key purpose is enzymatic reaction, which can be suppressed with high temperature; it is the oxidation reaction which should be concentrated on in the second stage, and a middle temperature is a good choice. With the designed fuzzy logic algorithm, the pot temperature is kept at a high level at the beginning, and the enzymatic reaction can be successfully reduced. In the second stage, the temperature is kept at a middle value to decrease oxidation. With the designed intelligent control, the green tea product quality is improved for aroma, taste, appearance, liquor color, and residues.

Biochemical investigation of Bangladeshi black tea and their correlation to organoleptic quality evaluation

Black tea samples were investigated for theaflavin (TF), thearubigin (TR), highly polymerized substances (HPS), total liquor color (TLC), color index (CI), caffeine (CAF), total polyphenol content (TPC), antioxidant activity (DPPH) and organoleptic evaluation. The objectives of the study was to perform the biochemical analysis and organoleptic test of various black tea and develop a correlation between them. Overall correlation study showed that TF:TR and total liquor color, are significantly (p < 0.01) and positively correlated with the total quality score where the correlation coefficient was 0.970 and 0.969 respectively. Additionally, statistical analysis revealed a significant (p < 0.01) and positive correlation between total phenol contents and antioxidant scavenging activity (0.986), supporting the notion that TPC accounts for most of the antioxidants in tea extract. Qualitative characteristics and organoleptic tests were shown identical outcomes in this study.

Non-Destructive and Non-Invasive Measurement of Ethanol and Toxic Alcohol Strengths in Beverages and Spirits Using Portable Raman Spectroscopy.

The measurement of ethanol and toxic alcohol (methanol and isopropanol) strengths in beverages and spirits is crucial for health reasons but also for the identification of adulterated products. Many methodologies have been reported in the literature, based mainly on chromatographic and on spectroscopic techniques. Chromatographic techniques are laborious and time-consuming, while spectroscopic techniques are rapid and need no special sample pretreatment. All techniques were only applied to off-line or at-line manner. In the present work, Raman spectroscopy was used for fast and non-destructive measurements. A "through the container" method was developed for a non-invasive analysis, i.e., analysis without unsealing the bottles. This method, coupled with a miniature portable Raman, can serve for in-line measurements in a production line. The optimum laser focus for maximum spirit signal and minimum glass-wall signal was investigated. Calibration curves for the alcohols of interest were constructed and validated. The limits of detections were calculated and proved to be lower than the legitimate values. The influences of the liquor color and the bottle color, shape, and thickness were checked. Twenty-eight alcoholic products were studied. The concentrations found were compared against the nominal values (from the bottle labels).

A roast-leach process for the recovery of vanadium from vanadium-bearing gasifier slag (VBGS) using citric acid as a green reagent: Leaching studies and statistical analysis for sustainable processing

The vanadium-bearing gasifier slag (VBGS) generated by petroleum industry as solid waste is considered as one of the valuable vanadium-containing resources. So, recovery of vanadium from VBGS is important from the environmental and industrial points of view. This work investigates vanadium leaching from VBGS at atmospheric pressure using a green reagent and achieves comprehensive utilization of the slag. Citric acid, an eco-friendly leachant, was used to recover V. Different leaching parameters were optimized and the maximum vanadium recovery of 98.8% was achieved at the optimum leaching condition of pH 1.5–1.7, temperature 75 °C, citric acid concentration 2.5%, pulp density 10% and leaching time 30 min. The leaching of vanadium showed that there were two stages in the kinetics of leaching. In the first 30 min, a sharp increase in the amount of vanadium extracted was observed and at longer times, leaching rate decreased. The blue colour of the leach liquor (LL) indicated that vanadium was leached in the form of V4+. The experimental data were employed as an input to the response surface methodology (RSM) to determine the best dependent/independent variables and predicted optimum condition to obtain maximum leaching efficiency. Finally, the present process results in the utilization of industrial waste to recover valuable metal ion and minimization of vanadium bearing primary resources.

A study on obstetric and perinatal outcome in a pregnancy complicated with oligohydramnios

Oligohydramnios, defined as when the AFI is less than 5. Definition of increased or decreased amniotic fluid volume are based on sonographic criteria. Oligohydramnios complicates approximately 1 to 2% of pregnancies Amniotic fluid provides the cushion effect against the constricting confines of the gravid uterus. It creates space and helps in musculoskeletal development of foetus, helps in normal fetal lung development and prevents compression of umbilical cord, placenta and hence protects the foetus from vascular and nutritional compromises. Oligohydramnios is associated with high risk adverse perinatal outcome like fetal distress, meconium aspiration, low APGAR, joint contracture, pulmonary hypoplasia etc., and associated with maternal morbidity in the form of increased rates of induction and/or operative interference. This study is conducted to find out the effects of oligohydramnios in determining the obstetric and perinatal outcome.To study, 1) Primary outcome- Obstetric outcome in terms of mode of delivery, indication for operative interference. 2) Secondary outcome- Neonatal outcome in terms of birth weight, Apgar score, colour of liquor, admissions to NICU. This is a prospective observational study conducted on 80 patients presenting to Department of Obstetrics and Gynecology at KIMS hospital, Bengaluru from January 2019 to June 2020 who were diagnosed with oligohydramnios with intact membranes with AFI&amp;#60;5cms using sonographic criteria. A total of 80 cases of Isolated Oligohydramnios were assessed. 38.7% cases delivered vaginally, 61.2% of cases underwent Cesarean section, 15% had meconium stained liquor, 15% had APGAR score of &amp;#60;7 at 1 minute, 42.5% had birth weight &amp;#60;2.5kgs and 37.5% of babies required NICU admissions. The present study was conducted to know the fetomaternal outcome in pregnancies with oligohydramnios. The study showed oligohydramnios was associated with higher rates of induction and Cesarean section and associated with Low birth weight babies.

Exploring the Anti-Inflammatory Mechanism of Tieguanyin (TGY) Volatile Compounds Based on Gas Chromatography-Mass Spectrometry (GCMS)- Network Pharmacology.

Inflammation is a common disease which can induce many diseases. There are unique advantages of Traditional Chinese Medicine (TCM) to anti-inflammation. Tieguanyin (TGY) is a well-known beverage; the quality is determined by aroma, taste, liquor color, and shape. The volatile compounds produce the flavor of tea, which can be lost with the increase of storage time. TGY has an excellent antiinflammatory effect; its volatile compounds also have an anti-inflammatory impact that is unclear. This study aimed to identify volatile compounds and anti-inflammatory mechanisms within the validity period (TGY1) and the out-of-date (TGY2). The volatile compounds of TGY1 and TGY2 were analyzed with headspace solid-phase microextraction (HS-PME) and identified by Gas chromatography-mass spectrometry (GC-MS). The percentage of volatile compounds was calculated by the peak area normalization method. The compounds of the targets were obtained from the Traditional Chinese Medicine Systems Pharmacology (TCMSP), PubChem Database, and Swiss Target Prediction database. Next, the disease potential targets were screened by the GeneCards database, Online Mendelian Inheritance in Man (OMM) database, and Therapeutic Target Database (TTD). Furthermore, core targets were screened by the Search Tool for the Retrieval of Inter-acting Genes/Proteins (STRING) database. Then, Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of core targets was performed by the ClueGo plugin Cytoscape 3.7.1 software. At last, Autodock vina software performed molecular docking between the main compounds and core targets. Significant differences in volatile compounds and percentage contents in TGY were observed; the 61 volatile compounds in TGY1 and 57 volatile compounds in TGY2 were identified. After excluding the unidentified compounds, a total of 47 volatile compounds were obtained from TGY1 and TGY2. With the use of network pharmacology, 34 core targets and 23 signaling pathways from TGY1, 28 core targets, and 19 signaling pathways from TGY2 were screened. The main common core targets of TGY1 and TGY2 contained MAPK3, TNF, MAPK1, SRC, etc., while the main different core targets included PTGS2, CAT, etc. A total of 12 biological processes are shared by TGY1 and TGY2, among which the cellular response to oxidative stress is the primary biological process. The different biological processes of TGY1 and TGY2 include cellular response to lipopolysaccharide, androgen receptor signaling pathway, etc. There were 14 common signaling pathways in TGY1 and TGY2, among which the thyroid hormone signaling pathway is the main common signaling pathway. The differential signaling pathways in TGY1 and TGY2 included the erbB signaling pathway, Chagas disease, etc. Molecular docking results showed that the ordinand and differential volatile compounds of TGY1 and TGY2 had different binding forces with the core targets. The GC-MS experiment showed significant differences in volatile compounds and percentage contents in TGY1 and TGY2. Network pharmacology indicated that they have anti-inflammatory effects. Besides, they were different in core targets, biological processes, and signaling pathways but shared similar anti-inflammatory mechanisms. Molecular docking results showed that the binding force of the TGY1 compounds to the core target is greater than that of the TGY2. Therefore, expired TGY affects volatile compounds, resulting in differences in the anti-inflammatory mechanism. The study provided a theoretical framework for further development and application of used medicinal and edible species. In addition, the application of expired TGY under safe conditions can also have anti-inflammatory effects. These results shed new light on the rational use of resources.

The correctness and completeness of documentation of parameters on the partographs used by midwives in primary healthcare facilities in midwestern Uganda: A retrospective descriptive study.

This study was conducted to determine the correctness and completeness of documentation of partographs. This was a retrospective descriptive study. We included 365 partographs of deliveries conducted from January 1st to October 31st 2019. Data were collected using a checklist and analysed descriptively. The study based on 13 partograph parameters. About 8-13 parameters were correctly documented in 71.5 % of the partographs. About 38.9%, 24.7%, 99.7%, 22.5% and 16 % of the partographs had no documentation of obstetric risk factors, foetal heart rate, colour of liquor, uterine contractions and cervical dilatation respectively. About 12.1% of the cervicographs crossed the action line and 61.4% of partographs where cervicographs crossed the action line had no documentation of action(s) taken.

Effect of fixation methods and various clones of Camellia sinensis var. sinensis (L) properties and antioxidant activity of Indonesian green tea

Fixation is essential in green tea processing to inactivate the polyphenol oxidase enzyme. In Indonesia, green tea is made from the Assam variety and produced using the panning method. Few studies are reported on green tea made from Indonesian clones of the Sinensis variety. This study aims to identify chemical characteristics, antioxidant activity, and sensory evaluation of green tea from local clones of the Sinensis variety (GMBS 2, GMBS 4, and GMBS 5) with different fixation methods (panning and steaming). The results show that the caffeine content of green tea products ranged from 2.51-2.59% and 2.67-2.74% for panning and steaming methods. The panning method produced green tea with higher total polyphenol and flavonoid content than the steaming method. Green tea with the panning method has an IC50 value of 14.45; 14.41; and 17.41 mg/L for GMBS 2, GMBS 4, and GMBS 5, respectively. The panning method resulted in a smaller IC50 value than the steaming method for GMBS 2 and GMBS 4 clones. The steaming method produced green tea with a higher taste, aroma, and total score than those the panning method. However, different fixation methods did not significantly affect the appearance, liquor color, and leaf infusion. In conclusion, different fixation methods on GMBS 2, GMB 4, and GMB 5 produced green tea products that met the Indonesian National Standard 3945:2016. Further research is needed to determine the role of the plucking period/season and the characteristics of volatile compounds of green tea from GMBS clones with different fixation methods.

Non-targeted metabolomics and electronic tongue analysis reveal the effect of rolling time on the sensory quality and nonvolatile metabolites of congou black tea

Rolling is a crucial step in congou black tea manufacturing. Herein, the influence of rolling time (50, 75, 100, 125 min) on the sensory quality and chemical composition of black tea was investigated by human sensory evaluation, electronic tongue, colorimetric analysis, tea pigments quantification and liquid chromatography-mass spectrometry (LC-MS) based non-targeted metabolomics. With increasing rolling time, tea liquor color attributes a*, b*, C* and pigments theaflavins (TFs), thearubigins (TRs), and theabrownins (TBs) were significantly increased. Fifty-two key differential metabolites were identified among four treatments. Catechins, dimeric/trimeric catechins, amino acids were significantly declined. The changes of flavonol/flavone glycosides depended on the linkage pattern. Flavonol O-glycosides such as myricetin 3-O-glucoside were decreased, whereas flavone C-glycosides such as vitexin were increased. These results suggested that different rolling times influenced the catechins oxidative polymerization, flavonol O-glycosides hydrolysis and amino acids oxidation. Black tea by 100-min rolling rendered optimal sensory quality, with highest umami and low bitter intensities in electronic tongue, and higher quality index (10 TFs + TRs)/TBs. Similar result was obtained in a validation batch in a different year. This study provides novel insights into the effect of rolling on black tea quality, which may serve as guidance for the manufacturing of high-quality congou black tea.

Influence of clone and nitrogen application level on quality of green tea in some selected tea (Camellia sinensis (L.)O. Kuntze) in Southwest Ethiopia

The biochemical constituents and organoleptic characteristics of Camellia sinensis (L.) O. Kuntze clones are not studied well in Ethiopia. The study aims to evaluate the polyphenols, caffeine, antioxidant content, and cup quality of clones at different nitrogen application rates and identify suitable clones and processes for daily consumable antioxidant-rich green tea. The experiment consisted of five clones (6/8, BB-35, 11/56, 11/4, and 12/38) and five nitrogen application rates (i.e., 0, 75, 150, 225, and 300 kg ha−1) under a split-plot design and was replicated three times. The biochemical constituents and overall quality of green tea were examined using HPLC, chemical analysis, and a cup taster. The results indicate that the polyphenol content increased slightly (P < 0.018) as the nitrogen application rate increased, with a weak correlation (r = 0.387). The caffeine content varies from 1.82 to 3.06%. Clone BB-35 scored the highest (3.06%), and clone 6/8 scored the lowest caffeine content in all nitrogen treatments. The total nitrogen content varied between 2.27 and 4.01 mg g−1 and slightly increased as the nitrogen application rate increased (r2 = 0.798). The antioxidant activity showed a significant variation (P < 0.001) among clones, and the tested clones had a high antioxidant inhibition percent ranging from 51.9 to 66.5%. The cup quality, aroma, liquor color, and dry leaf appearances of green tea were positively correlated (r = 0.68, r = 0.70, r = 0.48, and r = 0.30), and the overall quality of green tea improved as the nitrogen application rate increased. Clones 6/8 and 11/4 recorded the highest overall quality above the total mean value, whereas clones BB-35, 12/38, and 11/56 scored below the total mean value (77.68%). Clone 6/8 produced an excellent full aroma, balanced bitterness, and a slightly sweeter cup in all nitrogen treatments than other clones. Moreover, this clone contains less caffeine and high polyphenol content in high nitrogen treatment. The second-best, clone 11/4, has a good marketable green liquor, aroma, and dry-made tea appearance, but it is slightly bitterer than clone 6/8. The research concluded that clone 6/8 can produce a more acceptable and high-quality green tea at 300 kg N ha−1.

Monitoring Intrapartum Fetal Distress by Cardiotocography &amp; Observe its Relation with Umbilical Cord Blood Sampling &amp; Perinatal Outcome

Background: Cardiotocography (CTG) being a simple noninvasive tool has been used more frequently in recent decades to detect fetal distress &amp; to reduce neonatal mortality &amp;morbidity related to fetal hypoxia. On the other hand, umbilical cord blood gas can be used to detect fetal acidaemia due to fetal distress more accurately. This study aimed to correlate fetal monitoring findings by intrapartum CTG with umbilical cord blood pH &amp; lactate level &amp;test the ability of CTG to predict fetal distress &amp; neonatal outcome. Materials and Methods: This prospective cross-sectional observational study was conducted in the Department of Obstetrics and Gynecology of Chittagong Medical College Hospital, Chattogram, Bangladesh for one year from July 2020 to June 2021. It included 80 term singleton pregnancies in active labour. Intrapartum CTG was taken and classified into normal, suspicious and pathological according to FIGO guidelines of CTG monitoring 2015. Mode of delivery, liquor colour, Apgar scores at 1 and 5 minutes, and admission to the neonatal intensive care unit (NICU), adverse neonatal outcomes were observed. Immediately after delivery of the baby 10 cm of the umbilical cord was clamped doubly and 2-3 ml of umbilical cord arterial blood was taken immediately in a pre-heparinized syringe and sent to a laboratory for assessment of pH and lactate to detect fetal acidosis. Antenatal CTG was correlated to neonatal outcomes and cord blood acidosis by statistical analysis. Results: In this study, 40.0% of the women had normal CTG, 38.8% had suspicious CTG, and 21.2% had pathological CTG. There was a significant worsening of neonatal outcomes across these three groups concerning depressed (&lt;7) Apgar scores at 1 minute (40.6%, 80.6%, and 100%; P&lt;0.001), depressed Apgar scores at 5 minutes (3.1%, 22.6%, and 47.1%; P&lt;0.001), and admission to the NICU (9.4%, 38.6%, and 70.7%; P&lt;0.001). When CTG was pathological or suspicious CTG delivery by LSCS was 5.33 times higher (RR: 5.33; 95% CI: 2.09 -13.63) compared to subjects with normal CTG. There was also a progressive worsening of cord blood pH (7.25±0.05, 7.20±0.06, and 7.13±0.09; P&lt;0.001) and a progressive increase in lactate (3.66±1.01 mmol/l, 4.79±1.61 mmol/l, and 6.63±2.18 mmol/l; P&lt;0.001). Conclusions: It should be concluded that pathological CTG which correlates intrapartum fetal hypoxia with cord blood acidaemia and adverse neonatal outcomes. As cardiotocography is a simple, cost-effective noninvasive tool it can be used to detect fetal distress in labour. Continues CTG monitoring can be offered or recommended in every labour room setting to detect fetal distress &amp; early intervention to prevent neonatal morbidity &amp; mortality.

Effects of Fermentation Temperature and Time on the Color Attributes and Tea Pigments of Yunnan Congou Black Tea.

Yunnan Congou black tea (YCBT) is a typical black tea in China, and is rich in theaflavins (TFs), thearubigins (TRs), and theabrownins (TBs). However, the influence of the fermentation temperature and time on the liquor and appearance color and the correlation between the tea pigments and its color attributes remain unclear. We investigated the effects of the fermentation temperature and time on the color attributes and tea pigments of YCBT. A low fermentation temperature was beneficial to maintain a bright orange-red liquor color and promote the accumulation of TFs and TRs. In contrast, a high temperature gave the liquor a glossy appearance and was beneficial for the formation of TBs. A correlation analysis showed that the 10TFRB index best represented the contribution of tea pigments to the quality of black tea. Moreover, TRs and TBs content prediction models were established based on the liquor L and H values, where the former value can be used as an important index to judge the fermentation process. This study will further enrich the theory of black tea processing chemistry and provide technical support for the precise and directional production of black tea.