The Eagle Ni-Cu magmatic sulfide deposit in the Upper Peninsula of Michigan is hosted in mafic-ultramafic rocks associated with the 1.1 Ga Midcontinent Rift event. The deposit consists of massive, semi-massive and disseminated sulfide textural zones located in two closely spaced funnel-shaped intrusive bodies, called the Eagle and Eagle East intrusions. The intrusions are surrounded by metamorphosed supracrustal Paleoproterozoic rocks of the Michigamme Formation and at depth by a Neoarchean migmatitic banded gneiss. δ34S values were obtained from sulfide minerals separated by micro-drilling from different parts of the intrusions and the surrounding rocks. The δ34S values from the intrusions cluster within a small range between 1 and 3‰ relative to V-CDT regardless of the mode of occurrence, textural type, mineral type and location of the sample. However, disseminated sulfide minerals in the metasedimentary country rocks of the Michigamme Formation show a substantially greater range in δ34S values between 4 and 35‰ with a median of 7.7‰ while disseminated sulfides in the basement granite-gneiss show values ranging from −9 to 13‰ with a median of 2.5‰. The narrow range of δ34S values within the intrusions despite wide variabilities in the surrounding country rocks can be explained by the incorporation of crustal sulfur by a mantle-derived magma, followed by a homogeneous mixing of the immiscible sulfide liquid, with a silicate liquid to sulfide liquid ratio (R-factor) of 200 or more. The assimilation of crustal rocks and the mixing of the resultant immiscible sulfide liquid must have been facilitated by the rapid movement of magma along a narrow conduit system.