In the simultaneous measurement of liquid film temperature and thickness based on multi-wavelength absorption spectroscopy, selecting optimal wavelength combinations can significantly improve the measurement accuracy. In the work, the absorption spectra of e-liquid at different temperatures were measured firstly. And ten sets of two-wavelength and three-wavelength combinations were then established based on five specific wavelengths from the absorption spectra, respectively. And the measurement accuracy of all the combinations were validated with a home-made calibration system. Among them, an optimal three-wavelength combination were selected. Finally, the evaporation processes of e-liquid films at three initial thicknesses (921/780/629 μm) on a horizontal quartz plate were then investigated with the optimal combination. The variation trends of film temperature and thickness measured by the combination were consistent with imaging method and thermocouple. It was found that the optimal three-wavelength combination could achieve high accuracy in simultaneous measurement of liquid film temperature and thickness.