Liquid Chromatography Nuclear Magnetic Resonance Research Articles

Separation and identification of ribonuclease S-peptide methyl esters by ion-exchange high-performance liquid chromatography and 1H nuclear magnetic resonance spectroscopy

Separation and identification of ribonuclease S-peptide methyl esters by ion-exchange high-performance liquid chromatography and 1H nuclear magnetic resonance spectroscopy

On-line coupling of high-performance liquid chromatography and nuclear magnetic resonance

High-performance liquid chromatography—nuclear magnetic resonance (HPLC—NMR) on-line coupling is a possible solution to the problemof universal detectors in liquid chromatogrphy. By use of a newly developed 1H-FT-NMR* flow cell, 1H-NMR spectra of flowing systems cn be obtained. Although sensitivity and resolution are somewhat lower than in the conventional system, the 1H-MNMR spectra are of sufficient quality to enable classification of unknown compounds. The performed HPLC separation with on-line NMR-measurement shows the capabilities of the applied arrangement.