Lipoyl Domain Of E2 Subunit Research Articles

Biotin-related proteins of 70 and 72 kDa in gallbladder bile in primary biliary cirrhosis

Primary biliary cirrhosis (PBC) is an autoimmune liver disease characterized by serum antimitochondrial antibody mainly reacting with inner lipoyl domain of E2 subunit of pyruvate dehydrogenase (PDC-E2). Lipoyl domain of PDC-E2 has sequence homologies with biotinylated site of biotin enzymes. This biotin-related protein and the C355.1 reactive antigen were surveyed by Western blotting in gallbladder bile samples from 27 patients with PBC and from 23 patients with other hepatobiliary disease. Biotin-related proteins of 70 and 72 kDa were detected in 89% of PBC samples, whereas it was infrequently detected in 48% of control samples ( P<0.01). C355.1 reactive antigen of 64 kDa was detected in 22% of PBC samples and 8.7% of control samples ( P>0.01). This biotin-related proteins of 70 and 72 kDa may refer to the pathogenesis of PBC.