Production Of Lipopeptide Biosurfactant Research Articles

Solubilization and enhanced degradation of benzene phenolic derivatives-Bisphenol A/Triclosan using a biosurfactant producing white rot fungus Hypocrea lixii S5 with plant growth promoting traits.

Endocrine disrupting chemicals (EDCs) as benzene phenolic derivatives being hydrophobic partition to organic matter in sludge/soil sediments and show slow degradation rate owing to poor bioavailability to microbes. In the present study, the potential of a versatile white rot fungal isolate S5 identified as Hypocrea lixii was monitored to degrade bisphenol A (BPA)/triclosan (TCS) under shake flask conditions with concomitant production of lipopeptide biosurfactant (BS) and plant growth promotion. Sufficient growth of WRF for 5 days before supplementation of 50 ppm EDC (BPA/TCS) in set B showed an increase in degradation rates by 23% and 29% with corresponding increase in secretion of lignin-modifying enzymes compared to set A wherein almost 84% and 97% inhibition in fungal growth was observed when BPA/TCS were added at time of fungal inoculation. Further in set B, EDC concentration stimulated expression of laccase and lignin peroxidase (Lip) with 24.44 U/L of laccase and 281.69 U/L of Lip in 100 ppm BPA and 344 U/L Lip in 50 ppm TCS supplemented medium compared to their respective controls (without EDC). Biodegradation was also found to be correlated with lowering of surface tension from 57.02 mN/m (uninoculated control) to 44.16 mN/m in case of BPA and 38.49 mN/m in TCS, indicative of biosurfactant (BS) production. FTIR, GC-MS, and LC-ESI/MSMS confirmed the presence of surfactin lipopeptide isoforms. The WRF also displayed positive plant growth promoting traits as production of ammonia, indole acetic acid, siderophores, Zn solubilization, and 1-1-aminocyclopropane-1-carboxylate (ACC) deaminase activity, reflecting its soil restoration ability. The combined traits of biosurfactant production, EDC degradation and plant growth promotion displayed by WRF will help in emulsifying the hydrophobic pollutants favoring their fast degradation along with restoration of contaminated soil in natural conditions.

Production of Lipopeptide Biosurfactant Using Wastewater from Parboiled Paddy Rice and Evaluation of Antifungal Property of the Biosurfactant Against Two Dermatophyte Fungi.

A previously isolated lipopeptide biosurfactant-producing bacterium Bacillus licheniformis SCV1 was investigated for the production of the biosurfactant using wastewater from parboiled paddy rice. The biosurfactant thus produced was evaluated for its antifungal property against dermatophyte fungi Trichophyton ajelloi and Microsporum fulvum. Results revealed that the bacterial strain reduced surface tension of the media from 56.16 ± 1 mN/m to 35 ± 0.9 mN/m within 12h, which further shrank to 29.3 ± 1 mN/m in 24h of incubation. The yield of the biosurfactant was 3.15 ± 0.25g/L at 48h of incubation. The obtained biosurfactant exhibited efficient emulsifying activity against a wide range of hydrophobic substrates such as crude oil, olive oil, engine oil, and kerosene oil used in the study. The critical micelle concentration of the biosurfactant was found to be 80mg/L. Structural characterization using FT-IR and TLC revealed that the biosurfactant produced by the strain in the wastewater is a lipopeptide consisting of surfactin and iturin. LCMS analysis revealed that the surfactin homologs range from C12 to C17-surfactin while the iturin contains C13 to C17-iturin homologs. It also revealed an in vitro study that the biosurfactant has antifungal properties against dermatophyte fungi Trichophyton ajelloi and Microsporum fulvum. Microscopic observation of the hyphae of the treated dermatophyte revealed disruption and fissure of the mycelia. The chemical composition of the wastewater revealed that it contains adequate nutritional composition and micronutrients to support bacterial growth. This is the first report that the wastewater of parboiled paddy could be used as a low-cost substrate for the production of lipopeptide biosurfactant, and the biosurfactant could be used for preventing dermatophytes fungi.

Enhanced low-cost lipopeptide biosurfactant production by Bacillus velezensis from residual glycerin.

Biosurfactants (BSFs) are molecules produced by microorganisms from various carbon sources, with applications in bioremediation and petroleum recovery. However, the production cost limits large-scale applications. This study optimized BSFs production by Bacillus velezensis (strain MO13) using residual glycerin as a substrate. The spherical quadratic central composite design (CCD) model was used to standardize carbon source concentration (30g/L), temperature (34°C), pH (7.2), stirring (239rpm), and aeration (0.775vvm) in a 5-L bioreactor. Maximum BSFs production reached 1527.6mg/L of surfactins and 176.88mg/L of iturins, a threefold increase through optimization. Microbial development, substrate consumption, concentration of BSFs, and surface tension were also evaluated on the bioprocess dynamics. Mass spectrometry Q-TOF-MS identified five surfactin and two iturin isoforms produced by B. velezensis MO13. This study demonstrates significant progress on BSF production using industrial waste as a microbial substrate, surpassing reported concentrations in the literature.

Heavy metal application of response surface optimized-lipopeptide biosurfactant produced by Pseudomonas aeruginosa strain CGA-02 in low-cost substrate

Cost-effective methods of biosurfactant production with minimal environmental impact are needed as global demand continues to increase. This study evaluated lipopeptide biosurfactant production in a Pseudomonas aeruginosa strain CGA-02 using a low-cost carbon substrate. The structural properties of the biosurfactant and applicability of the biosurfactant in heavy metal removal were evaluated. Response surface methodology (RSM) involving central composite design (CCD) was used to optimize process parameters to maximize biosurfactant production. The study identified sugar cane molasses and sodium nitrate as carbon and nitrogen sources of choice for bacterial growth and biosurfactant production, with a relatively 2.64-fold increase in biosurfactant yield under optimized conditions. Analysis of the biosurfactant measured a surface tension reduction of water from 72.2 ± 0.26 to 30.5 ± 0.2 mN/m at 40 mg/L critical micelle concentration. GC–MS and FTIR analysis revealed structural properties of the lipopeptide biosurfactant, with fatty acid components predominantly 9-octadecenoic acid (oleic acid), n-hexadecanoic acid, cyclotetrasiloxane and trimyristin, and infrared peaks belonging to amine, carboxyl, nitrile, alkanol, ether and carbonyl groups. Capture of heavy metals using the biosurfactant was evaluated in soil microcosms. Removal rates of 80.47, 100, 77.57, 100, and 97.57% were recorded for As, Pb, Hg, Cd and Cr respectively after 12 weeks of incubation. There was no significant difference (p < 0.05) in the removal efficiency of the biosurfactant and an analogous chemical surfactant, sodium dodecyl sulphate. First and second-order kinetic models described heavy metal removal rates by the biosurfactant. We demonstrate the production of a useful biosurfactant using low-cost waste carbon.

Optimization of Lipopeptide Biosurfactant Production with Anti-Inflammatory and Antimicrobial Activities

Background:: Biosurfactants are very important amphiphile compounds due to their interesting advantages such as low toxicity, biodegradability and also their many biological properties. Methods:: In the present study, antimicrobial and anti-imflammatory activities were evaluated to determine the biological proprieties of biosurfactant BLA 2906 produced by Alcaligenes aquatilis YGD 2906 using different assays. Screening and optimization component concentrations in the medium were investigated using PBD and SRM to increase surfactant yield in term emulsification activity value (E24%). Results:: The halos of antifungal activity presented a mean value of 12.33 mm to 17.67 mm. For antibacterial activity, the diameter varied from 10.33 to 12.67 mm with a very important antiinflammatory activity using a protein denaturation method that showed a maximum inhibition of 92.79%. Conclusion:: These results suggest that BLA 2906 may be used as a new therapeutic and antiinflammatory agent. The PBD selected 7 significant components out of the 14 screened. The RSM resulted in the production in terms of emulsification activity of 68.37% in the optimized medium.

Efficient conversion of black cumin cake from industrial waste into lipopeptide biosurfactant by Pseudomonas fluorescens

Most biosurfactants are obtained using costly culture media, which limits their wider industrial use. In the present study, a low-cost culture medium, containing the agro-industrial residue black cumin cake, was developed for amphisin production by Pseudomonas fluorescens DSS73. By using black cumin cake as the substrate, not only was the production cost reduced but also a higher production yield was achieved. A Box-Behnken experimental design was applied to maximize lipopeptide biosurfactant production. The optimal conditions for amphisin production, such as black cumin cake (6.6%) and NaCl (8.0 mM) concentration, and cultivation time (6.5 days), were determined. Yield of amphisin production, performed in optimal conditions, reached 16.51 ± 0.49 g/L. Such high production has not been evidenced previously for Pseudomonas lipopeptide biosurfactants. Moreover, active utilization of the substrate, observed with the aid of scanning electron microscopy (SEM), documented by numerous holes and pitting on the black cumin cake surface, was confirmed. Finally, antifungal activity of amphisin against Aspergillus carbonarius was demonstrated. Hence, amphisin production by P. fluorescens was achieved with statistical optimization using an inexpensive agro-industrial by-product for the first time.

Optimization of Lipopeptide Biosurfactant Production by Salibacterium sp. 4CTb in Batch Stirred-Tank Bioreactors.

Halophilic microorganisms are potentially capable as platforms to produce low-cost biosurfactants. However, the robustness of bioprocesses is still a challenge and, therefore, it is essential to understand the effects of microbiological culture conditions through bioreactor engineering. Based on a design of experiments (DOE) and a response surface methodology (RSM) tailored and taken from the literature, the present work focuses on the evaluation of a composite central design (CCD) under batch cultures in stirred-tank bioreactors with the halophilic bacteria Salibacterium sp. 4CTb in order to determine the operative conditions that favor mass transfer and optimize the production of a lipopeptide. The results obtained showed profiles highlighting the most favorable culture conditions, which lead to an emulsification index (E24%) higher than 70%. Moreover, through the behavior of dissolved oxygen (DO), it was possible to experimentally evaluate the higher volumetric coefficient of mass transfer in the presence of lipopeptide (kLa = 31 1/h) as a key criterion for the synthesis of the biosurfactant on further cell expansion.

Potential of Enterococcus faecium LM5.2 for lipopeptide biosurfactant production and its effect on the growth of maize (Zea mays L.).

This study characterized the lipopeptide biosurfactants produced by lactic acid bacteria isolated from milk and milk products, in addition to their effect on maize plant growth. The oil displacement test was performed as a primary screening method to select the BS producing bacteria. The strain LM5.2 had the maximum emulsification index of 45.1 ± 3, reduced the surface tension to 32.98 ± 0.23% among all the isolates, and efficiently produced 945.26 ± 4.62mg/l of biosurfactants within 48h in MRS broth under the optimum conditions. The isolate LM5.2 was identified using physiochemical tests and 16S rRNA gene sequencing as E. faecium. The biosurfactant was purified by TLC and identified as lipopeptide-like iturins and surfactins based on Rf values. Mass spectroscopy, NMR, and FTIR analysis also confirmed the biosurfactant's identity as the derivatives of iturin and surfactin. In vitro biosurfactant application significantly enhanced seedling growth at an optimal concentration of 450µg/ml. Plant treatment (pot experiment) with E. faecium LM5.2 significantly promoted Zea mays growth compared to the control. To the best of our knowledge, this is the first report of lipopeptide biosurfactant production by E. faecium. Moreover, the study also showed that the biosurfactant and biosurfactant-producing E. faecium LM5.2 could be an eco-friendly plant growth-promoting agent.

Formulation of a Culture Medium to Optimize the Production of Lipopeptide Biosurfactant by a New Isolate of Bacillus sp.: A Soil Heavy Metal Mitigation Approach.

This research aimed to optimize a lipopeptide biosurfactant produced from Bacillus sp. SHA302 due to its high efficiency of heavy metal release in soil. The results demonstrated that the metal release capacity of the lipopeptide biosurfactant alone increased with increasing the biosurfactant concentration. Among treatments with different biosurfactant concentrations plus acid, the highest metal release rates of 53.8% ± 1.4 and 39.3% ± 1.7 for Zn and Pb, respectively, were observed in the critical micelle concentration (CMC) + HCl treatment. The results of a factorial experiment designed for optimizing biosurfactant production showed that among five inexpensive carbon sources and six mineral nitrogen sources, sugar beet molasses (1%) and ammonium chloride (0.1%) were the most efficient sources in lowering the surface tension (ST) of the culture media to 32.2 ± 0.76 mN/m. The second step of the experiment was a Plackett–Burman design with 11 factors and showed that the four factors of pH, ammonium chloride, magnesium sulfate, and molasses significantly affected (P < 0.05) the changes in ST and biosurfactant production. The third step of the experiment was done using the response surface methodology (RSM) with a central composite design. The results showed that a pH of 7.3, 1.5 g/l of ammonium chloride, 0.3 g/l of magnesium sulfate, and 10% of sugar beet molasses yielded values of 29.2 ± 0.71 mN/m and 5.74 ± 0.52 g/l for the two variables of ST and biosurfactant production, respectively, which reached their most optimal levels.

Production of lipopeptide biosurfactant by Bacillus subtilis GY19 and its application as oil-contaminated surface cleaning agent

Production of lipopeptide biosurfactant by Bacillus subtilis GY19 and its application as oil-contaminated surface cleaning agent

Optimization of lipopeptide biosurfactant production by Bacillus licheniformis L20 and performance evaluation of biosurfactant mixed system for enhanced oil recovery

Biosurfactant is an important amphiphilic microbial product, which plays an important role in microbial enhanced oil recovery (MEOR) by reducing crude oil-water interfacial tension (IFT), emulsification and wettability alternation. Compared with chemical surfactants, biosurfactants have the advantages of lower toxicity and higher biodegradability, but the relatively low yield also limits their applications. In this study, the influence of hydrocarbon carbon sources on the production of biosurfactants by Bacillus licheniformis L20 was investigated, and the variable of unit biosurfactant concentration was proposed to optimize the biosurfactant production medium. The highest concentration was obtained in LB/MSS-K medium, which was up to 1.225 g/L. The biosurfactant produced by Bacillus licheniformis L20 in that medium was characterized as lipopeptide via using FTIR. This biosurfactant shown stable emulsifying properties for different hydrocarbons and crude oils, but it can not reduce the oil-water IFT significantly. However, after mixed biosurfactant with chemical anionic surfactants (1,3-diacylglycerols-2-sulfate) and nonionic surfactant (fatty alcohol-polyoxyethylene ether), the anionic surfactant/biosurfactant mixed system can reduce the crude oil-water IFT to 0.109 mN/m. In addition, the emulsification stability of the biosurfactant mixed system were determined, the results shown that the emulsion formed by the nonionic surfactant/biosurfactant mixed system or the anionic surfactant/biosurfactant mixed system can remain stable under a wide range of temperature, pH and NaCl concentration, respectively. Furthermore, the performance of chemical surfactant/biosurfactant mixed system were evaluated, the results shown that anionic surfactant/biosurfactant mixed system and nonionic surfactant/biosurfactant mixed system can increase the oil recovery rate by 24.12% and 21.67% respectively after water flooding, while the biosurfactant used alone was only 19.58%. Thus, the results obtained from this work shown that anionic surfactant/biosurfactant mixed system have good prospect of application in ex-situ MEOR. • Effect of hydrocarbon carbon sources on biosurfactants production was investigated. • Interfacial activity of anionic or nonionic/biosurfactant mixed system was studied. • These mixed system led to higher oil recovery rate than biosurfactant used alone.

Surface properties and heavy metals chelation of lipopeptides biosurfactants produced from date flour by Bacillus subtilis ZNI5: optimized production for application in bioremediation

The present study summarizes the valorization of date flour by the production of lipopeptide biosurfactant (BioS) by Bacillus subtilis ZNI5 (MW091416). A Taguchi design permitted the formulation of a medium composed only of 6% date flour and 0.5% yeast extract within 2days of incubation at 150rpm with a maximal surface tension (ST) reduction of about 27.8 mN/m. The characterization of the lipopeptide shows a CMC value of about 400mg/L with a minimal ST of 30 mN/m and an ability to disperse oil to about 80mm at 800mg/L. Having reduced phytotoxicity, the ZNI5 BioS and ZNI5 strain were assayed for Copper and Cobalt chelation and biosorption. The improvement of the germination index of radish seeds irrigated by the treated contaminated water showed the great potential application of ZNI5 lipopeptide in the bioremediation of heavy metals.

Production of surfactin by Bacillus subtilis LAMI005 and evaluation of its potential as tensoactive and emulsifier

AbstractIn this study, the kinetic of lipopeptide biosurfactant production by Bacillus subtilis LAMI005 and its tensoactive and emulsifier properties were evaluated. The influence of the concentrations of nitrogen ((NH4)2SO4) and carbon source (glucose and fructose) on biosurfactant production, using a mineral medium, were evaluated. A production of about 930 mg · L−1 of surfactin was observed for mineral medium supplied with total reducing sugars (16.55 g · L−1) and (NH4)2SO4 (1 g · L−1) as carbon and nitrogen sources, respectively. The crude biosurfactant exhibited its potential as tensoactive, presenting the capability to reach surface tension of about 28 mN ⋅ m−1 for water and exhibiting low CMC (~14–20 mg · L−1). The analysis of emulsifying activity showed a greater stability of emulsion formed in motor oil (3.5 U), soybean oil (2.0 U), and biodiesel (1.5 U). The emulsion formulated on motor oil remained stable for more than 200 h, which was explained by its average drop diameter (~0–10 μm). The surface‐active properties of surfactin did not seem to be affected by different NaCl concentrations, which may promote its applicability in high salt environments, such as oil spills in marine areas.

Production of lipopeptide biosurfactant by Kurthia gibsonii KH2 and their synergistic action in biodecolourisation of textile wastewater

Textile dyes are recalcitrant molecules and contain a high level of chemicals and colour which poses a serious challenge to surrounding environments. Therefore, this study aims to produce biosurfactant and investigate the synergistic action on decolourisation of textile dyes by the combination of bacteria and biosurfactant. An effective dye degrading strain and biosurfactant-producer, Kurthia gibsonii KH2, was isolated from textile wastewater using molasses as the only source of carbon and energy. The isolates were identified and screened for biosurfactant production using haemolytic activity, oil spreading technique, drop collapse test and emulsification index. Fourier Transform Infrared Spectrum (FTIR) and Thin Layer Chromatography (TLC) analyses were carried out to detect the type of biosurfactant. The effect of different physicochemical parameters on textile wastewater decolourisation was assessed within 24 h. The Kurthia gibsonii KH2 showed positive results for haemolytic activity, oil spreading technique, and drop collapse test. The emulsification test (E24) revealed that Kurthia gibsonii KH2 had a higher emulsification index of 63%. FTIR and TLC analyses indicated that the biosurfactant was a lipopeptide and was formed with a yield of 2 gL −1 . The synergistic activity of Kurthia gibsonii KH2 and lipopeptide biosurfactant resulted in decolourisation levels of 85% at 100 mg/L concentration and pH 7 was recorded at 168 h of incubation. The high attributes of these combinations and the phytotoxicity tests implied that the metabolites were less toxic, making it a promising option for the biodecolourisation and biodegradation of industrial textile wastewater and various environmental conditions. • Textile wastewater treatment using utilisation of microbial biocatalysts is vital. • The lipopeptide biosurfactant was derived from the bacteria Kurthia gibsonii KH2. • The strain showed synergistic action in textile dyes removal efficiency. • Decolorisation process was the dominant removal mechanism.

Production of lipopeptide biosurfactant in batch and fed-batch Streptomyces sp. PBD-410L cultures growing on palm oil.

The present study focused on lipopeptide biosurfactant production by Streptomyces sp. PBD-410L in batch and fed-batch fermentation in a 3-L stirred-tank reactor (STR) using palm oil as a sole carbon source. In batch cultivation, the impact of bioprocessing parameters, namely aeration rate and agitation speed, was studied to improve biomass growth and lipopeptide biosurfactant production. The maximum oil spreading technique (OST) result (45mm) which corresponds to 3.74g/L of biosurfactant produced, was attained when the culture was agitated at 200rpm and aeration rate of 0.5 vvm. The best aeration rate and agitation speed obtained from the batch cultivation was adopted in the fed-batch cultivation using DO-stat feeding strategy to further improve the lipopeptide biosurfactant production. The lipopeptide biosurfactant production was enhanced from 3.74 to 5.32g/L via fed-batch fermentation mode at an initial feed rate of 0.6mL/h compared to that in batch cultivation. This is the first report on the employment of fed-batch cultivation on the production of biosurfactant by genus Streptomyces.

Bioremediation of 2,4-Diaminotoluene in Aqueous Solution Enhanced by Lipopeptide Biosurfactant Production from Bacterial Strains

AbstractThis research concentrated on the isolation and preparation of biosurfactant-producing bacterial strains for the degradation of aromatic amine compound 2,4-diaminotoluene (2,4-DAT). Three s...

Enhancement of Paenibacillus sp. D9 Lipopeptide Biosurfactant Production Through the Optimization of Medium Composition and Its Application for Biodegradation of Hydrophobic Pollutants.

Interests in biosurfactant in industrial and environmental applications have increased considerably in recent years, owing to their potential benefits over synthetic counterparts. The present study aimed at analyzing the stability and oil removal efficiency of a new lipopeptide biosurfactant produced by Paenibacillus sp. D9 and its feasibility of its use in biotechnological applications. Paenibacillus sp. D9 was evaluated for optimal growth conditions and improved production yield of lipopeptide biosurfactant with variations in different substrate parameters such as carbon (C), nitrogen (N), C:N: ratio, metal supplements, pH, and temperature. Enhanced biosurfactant production was observed when using diesel fuel and ammonium sulfate as carbon and nitrogen source respectively. The maximum biosurfactant yield of 4.11g/L by Paenibacillus sp. D9 occurred at a C/N ratio of 3:1, at pH 7.0, 30°C, 4.0mM MgSO4, and 1.5% inoculum size. The D9 biosurfactant was found to retain surface-active properties under the extreme conditions such as high thermal, acidic, alkaline, and salt concentration. The ability to emulsify further emphasizes its potential usage in biotechnological application. Additionally, the lipopeptide biosurfactant exhibited good performance in the degradation of highly toxic substances when compared with chemical surfactant, which proposes its probable application in biodegradation, microbial-enhanced oil recovery or bioremediation. Furthermore, the biosurfactants were effective in a test to stimulate the solubilization of hydrophobic pollutants in both liquid environments removing 49.1 to 65.1% diesel fuel including hydrophobic pollutants. The study highlights the usefulness of optimization of culture parameters and their effects on biosurfactant production, high stability, improved desorption, and solubilization of hydrophobic pollutants.

Enhanced Biosurfactant Production by Bacillus pumilus 2IR in Fed-Batch Fermentation Using 5-L Bioreactor

The enhancement of the production of lipopeptide biosurfactant by Bacillus pumilus 2IR as an oil field-isolated bacterium was investigated in the fed-batch fermentation using a 5-L bioreactor. The initial study on the culture medium composition used for biosurfactant synthesis revealed that biosurfactant production was greatly affected by glucose, crude oil, potassium nitrate and ammonium sulfate as pivotal carbon and nitrogen sources which were utilized for biosurfactant synthesis in the bioreactor. The results obtained from the batch fermentation process in the bioreactor showed that the quantities of biomass and biosurfactant produced were 4.15 and 0.98 g/L, respectively. Similar results revealed that fed-batch fermentation led to the production of 5.71 g/L biomass and 1.06 g/L biosurfactant in the bioreactor.

Production of Lipopeptide Biosurfactant by a Marine Nesterenkonia sp. and Its Application in Food Industry.

Biosurfactants are smart biomolecules which have wide spread application in medicines, processed foods, cosmetics as well as in bioremediation. In food industry, biosurfactants are used as emulsion stabilizing agents, antiadhesives, and antimicrobial/antibiofilm agents. Nowadays biosurfactant demands in industries has increased tremendously and therefore new bacterial strains are being explored for large scale production of biosurfactants. In this study, an actinobacterial strain MSA31 was isolated from a marine sponge Fasciospongia cavernosa which showed high activity in biosurfactant screening assays such as drop collapsing, oil displacement, lipase and emulsification. Lipopeptide produced by MSA31 was found to be thermostable which was evident in differential scanning calorimetry analysis. The spectral data obtained in the Fourier transform infrared spectroscopy showed the presence of aliphatic groups combined with peptide moiety which is a characteristic feature of lipopeptides. The stability index of lipopeptide MSA31 revealed “halo-alkali and thermal tolerant biosurfactant” which can be used in the food industry. Microtiter plate assay showed 125 μg/ml of lipopeptide was effective in reducing the biofilm formation activity of pathogenic multidrug resistant Staphylococcus aureus. The confocal laser scanning microscopic images provided further evidences that lipopeptide MSA31 was an effective antibiofilm agent. The antioxidant activity of lipopeptide MSA31 may be due to the presence of unsaturated fatty acid present in the molecule. The brine shrimp cytotoxicity assay showed lipopeptide MSA31 was non-toxic and can be used as food additives. Incorporation of lipopeptide MSA31 in muffin showed improved organoleptic qualities compared to positive and negative control. This study provides a valuable input for this lipopeptide to be used in food industry as an effective emulsifier, with good antioxidant activity and as a protective agent against S. aureus.

Anaerobic lipopeptide biosurfactant production by an engineered bacterial strain for in situ microbial enhanced oil recovery

Anaerobic lipopeptide biosurfactant production by engineered bacterial strain FA-2 was fulfilled forin situMEOR potential application.