Optimization of Lipopeptide Biosurfactant Production with Anti-Inflammatory and Antimicrobial Activities

Abstract

Background:: Biosurfactants are very important amphiphile compounds due to their interesting advantages such as low toxicity, biodegradability and also their many biological properties. Methods:: In the present study, antimicrobial and anti-imflammatory activities were evaluated to determine the biological proprieties of biosurfactant BLA 2906 produced by Alcaligenes aquatilis YGD 2906 using different assays. Screening and optimization component concentrations in the medium were investigated using PBD and SRM to increase surfactant yield in term emulsification activity value (E24%). Results:: The halos of antifungal activity presented a mean value of 12.33 mm to 17.67 mm. For antibacterial activity, the diameter varied from 10.33 to 12.67 mm with a very important antiinflammatory activity using a protein denaturation method that showed a maximum inhibition of 92.79%. Conclusion:: These results suggest that BLA 2906 may be used as a new therapeutic and antiinflammatory agent. The PBD selected 7 significant components out of the 14 screened. The RSM resulted in the production in terms of emulsification activity of 68.37% in the optimized medium.