Microalgae Lipid Research Articles

Effect of pH, Temperature, and CO2 Concentration on Growth and Lipid Accumulation of Nannochloropsis sp. MASCC 11

This study determined growth and lipid accumulation in Nannochloropsis sp. MASCC 11 cultivated at different pH, temperatures, and CO2 concentrations in 10-day period. The suitability for biodiesel production was also evaluated based on the fatty acid profiles of microalgae lipid. Nannochloropsis sp. MASCC 11 showed an excellent tolerance to acidic pH (as low as 4.0), high temperatures (at least 40°C), and high CO2 concentrations (5%–15%), which are major stressed conditions in flue gas. The highest algal biomass was acquired at pH of 9.0 (0.44 gL−1), a temperature of 35C (0.63 g L−1), and a CO2 concentration of 5% (2.27gL−1). Maximum lipid production was obtained at pH of 6.0 (108.2 mg L−1), a temperature of 35°C (134.6mgL−1), and a CO2 concentration of 5% (782.7mg L−1). Synthesis of polyunsaturated fatty acids (PUFAs) in biomass was stimulated under high CO2 concentrations, remaining above 80% of total fatty acids, mainly composed of C16:3, C18:2, and C18:3. This led to the algae-based biodiesel having a lower oxidation stability, better cold flow properties, and other parameters such as its kinematic viscosity, cetane number, and specific gravity complied with ASTM or EN 14214 biodiesel specifications. Therefore, the improvement of oxidative stability needs to be considered before Nannochloropsis sp. MASCC 11 lipid can be used for biodiesel production, even if this species can grow well under stressful conditions.

Study Literature of Extraction Lipid Method from Microalgae Nannochloropsis sp

Biodiesel is a promising alternative fuel obtained from plant oils and animal fats through the esterification process with alcohol. Lipid microalgae Nannochloropsis sp. can be used as raw material for the production of biodiesel or biofuel with oil content in the microalgae Nannochloropsis sp. as much as 21.3 - 32.7% so, it has the potential to be used as a raw material in making biodiesel. Biodiesel produced from microalgae biomass is the most suitable alternative fuel for the future and environmentally friendly fuel. In microalgae lipid extraction, Nannnochloropsis sp. using several process aids such as autoclave, microwave, and soxhlet. Different types of solvents are used to determine the% yield of lipids present in microalgae. Extraction was carried out to compare the extraction method using autoclave, microwave, and soxhlet to obtain the best method that produced the most substantial % lipid. An autoclave obtained the most abundant lipid yield with n-hexane solvent with a ratio of 3: 1 solvent and algal oil. Thus, this review describes the lipid extraction of the microalgae Nannochloropsis sp. with various extraction aids and different solvent types related to lipid production over the past few years.

Enhanced microalgae biomass and lipid output for increased biodiesel productivity

This paper investigated the cumulative impact of salinity, carbon source (glycerol and glucose) and photoperiod on the cultivation of the microalga Phaeodactylum tricornutum in mixotrophic growth in pure air supplied photobioreactors aiming at biomass output and lipid content enhancement for increased biodiesel productivity. For that, Phaeodactylum tricornutum was grown for 18 and 20 days in modified F2 medium which was supplied in the beginning of the culture, and with daily addition of glycerol (0.1 M) or glucose (0.05 M); salinity at 15 and 30‰, with the luminosity of 165 μmols photons m−2s−2 for 24 h day−1 or partial 12 h day−1. Biomass production was registered, total lipids quantified with the Bligh and Dyer methodology, and the lipids’ drops observed with Nile Red staining. Regarding salinity, a value of 15‰ led to the highest microalgae growth. Glycerol 0.1 M was the carbon source which provided the best assimilation by the microalgae, reaching up to 1.3 g L−1 of biomass. The 24 h-illumination photoperiod with glycerol in mixotrophic cultivation led to 338.97 mg L−1 of lipids, which was roughly 80% higher than the lipid content obtained with autotrophic growth. In conclusion, the most effective conditions were glycerol 0.1 M (carbon source), 15‰ salinity, and 24 h-illumination.

Simultaneous accumulation of lipid and carotenoid in freshwater green microalgae Desmodesmus subspicatus LC172266 by nutrient replete strategy under mixotrophic condition

Production of biofuel by microalgae can become economically viable if other high-value commercial products such as carotenoid are simultaneously produced. This study aimed at enhancement of lipid and carotenoid productivity by an oleaginous microalga sp. Desmodesmus subspicatus LC172266 by supplementing Nitrogen sources to BG 11 medium under batch or fed batch mixotrophic condition. Optimum urea supplement (0.2 gL−1) to BG 11 medium enhanced carotenoid content of the D. subspicatus by about 3.7 fold (3.02±0.002 mgg−1 cell) and 3.0 fold (3.86±0.001 mgg−1 cell) using glucose or glycerol as organic carbon sources, respectively. Also, lipid productivity was enhanced by about 2.0 (0.07817±0.0028 gL−1day−1) (0.265±0.006 gg−1 cell) and 2.5 (0.08426±0.0008 gL−1day−1) (0.285±0.02 gg−1 cell) fold. Fed-batch cultivation with glucose at a feed rate of 0.5 gL−1 per day enhanced both carotenoid and lipid productivities of D. subspicatus by 34% and 45%, respectively, after eight days of mixotrophic cultivation.

Regulation and remodeling of intermediate metabolite and membrane lipid during NaCl-induced stress in freshwater microalga Micractinium sp. XJ-2 for biofuel production.

Microalgae can accumulate a large fraction of reduced carbon as lipids under NaCl stress. This study investigated the mechanism of carbon allocation and reduction and triacylglycerol (TAG) accumulation in microalgae under NaCl-induced stress. Micractinium sp. XJ-2 was exposed to NaCl stress and cells were subjected to physiological, biochemical, and metabolic analyses to elucidate the stress-responsive mechanism. Lipid increased with NaCl concentration after 0-12 hr, then stabilized after 12-48 hr, and finally decreased after 48-72 hr, whereas TAG increased (0-48 hr) and then decreased (48-72 hr). Under NaCl-induced stress at lower concentrations, TAG accumulation, at first, mainly shown to rely on the carbon fixation through photosynthetic fixation, carbohydrate degradation, and membrane lipids remodeling. Moreover, carbon compounds generated by the degradation of some amino acids were reallocated and enhanced fatty acid synthesis. The remodeling of the membrane lipids of NaCl-induced microalgae relied on the following processes: (a) Increase in the amount of phospholipids and reduction in the amount of glycolipids and (b) extension of long-chain fatty acids. This study enhances our understanding of TAG production under NaCl stress and thus will provide a theoretical basis for the industrial application of NaCl-induced in the microalgal biodiesel industry.

Green technology for the industrial production of biofuels and bioproducts from microalgae: a review

Rise in human population and gradual decline in fossil fuels are increasing the demand for fuels and causing an upsurge in fuel prices and global warming. Concomitantly, consumers have raised their health awareness by taking various supplements, e.g. omega-3 fatty acids, thus calling for advanced nutraceuticals. Both issues are addressed by recent research on microalgae, which can be easily cultivated to produce lipidic biofuels and active drugs. Here, we reviewed the types and biosynthesis of microalgal lipids. We discuss genetic engineering and manipulation of cultivation conditions for enhancing lipid production. We present techniques for lipid extraction, with focus on green techniques. We also discuss the technical and economic challenges in manufacturing microalgae-based biofuels and other bioproducts at the industrial scale. Last, the market potentials and further research directions for future commercialization of microalgae lipids are discussed.

Microalgae: A Promising Source of Valuable Bioproducts.

Microalgae are a group of autotrophic microorganisms that live in marine, freshwater and soil ecosystems and produce organic substances in the process of photosynthesis. Due to their high metabolic flexibility, adaptation to various cultivation conditions as well as the possibility of rapid growth, the number of studies on their use as a source of biologically valuable products is growing rapidly. Currently, integrated technologies for the cultivation of microalgae aiming to isolate various biologically active substances from biomass to increase the profitability of algae production are being sought. To implement this kind of development, the high productivity of industrial cultivation systems must be accompanied by the ability to control the biosynthesis of biologically valuable compounds in conditions of intensive culture growth. The review considers the main factors (temperature, pH, component composition, etc.) that affect the biomass growth process and the biologically active substance synthesis in microalgae. The advantages and disadvantages of existing cultivation methods are outlined. An analysis of various methods for the isolation and overproduction of the main biologically active substances of microalgae (proteins, lipids, polysaccharides, pigments and vitamins) is presented and new technologies and approaches aimed at using microalgae as promising ingredients in value-added products are considered.

Effects of Electro-fermentation on Increasing Lipid Extraction from Schizochytrium

In this study, electro-fermentation was used as one of the ways to break the wall of microalgal biomass. Schizochytrium is a marine microalga rich in lipids. Chloroform and methanol are excellent solvents for extracting lipids from microalgae in that they can surmount the obstacles of cell walls, but they are too contaminated and exorbitant and are not suitable for large-scale fuel production. For the purpose of improving the extraction rate of microalgae lipid, utilizing electro-fermentation, the crude lipids content was increased by 55.6%, the lipids extraction efficiency was increased by 1373% after extraction with a series of solvents. Compared with 100% Bligh & Dyer, 80% lipids yield can be obtained by using only a small amount of chloroform, methanol and isopropanol.This study demonstrates that electro-fermentation could be an effective and energy-saving method of increasing lipid extraction efficiency from Schizochytrium.

Stresses as First-Line Tools for Enhancing Lipid and Carotenoid Production in Microalgae.

Microalgae can produce high-value-added products such as lipids and carotenoids using light or sugars, and their biosynthesis mechanism can be triggered by various stress conditions. Under nutrient deprivation or environmental stresses, microalgal cells accumulate lipids as an energy-rich carbon storage battery and generate additional amounts of carotenoids to alleviate the oxidative damage induced by stress conditions. Though stressful conditions are unfavorable for biomass accumulation and can induce oxidative damage, stress-based strategies are widely used in this field due to their effectiveness and economy. For the overproduction of different target products, it is required and meaningful to deeply understand the effects and mechanisms of various stress conditions so as to provide guidance on choosing the appropriate stress conditions. Moreover, the underlying molecular mechanisms under stress conditions can be clarified by omics technologies, which exhibit enormous potential in guiding rational genetic engineering for improving lipid and carotenoid biosynthesis.

Biodiesel production, through intensification and profitable distribution of fatty acid methyl esters by a microalgae-yeast co-culture, isolated from wastewater as a function of the nutrients’ composition of the culture media

Due to expensive culture realizations and low lipid yields, microalgae-based biodiesel production still lacks economic viability at an industrial-scale. Optimization of lipids and fatty acid methyl esters (FAMEs) distribution is a necessary condition to get sustainable microalgae biodiesel production. Nutritional sources (nitrogen, phosphorous, carbon and iron) influence the lipid accumulation and biomass production yield. The aim of this study is to get high-quality biodiesel by a microalgae-yeast co-culture based on the selection of culture media micronutrients’ distribution. A response surface-based combination of NaNO3, K2HPO4 and FeSO4*7H2O nutrients increases biomass and lipids accumulations. The obtained highest biomass and lipids contents are 1.68 gL−1 and 27.77%, respectively. Under the selected optimized micronutrients’ composition, high-saturated fatty acids are obtained if high ratio of N/P (6:1 and 11:1) and unsaturated fatty acid under N/P ratio of (9:1) are considered. These results suggest microalgae-yeast co-culturing under controlled N/P ratio in the culture medium produce desirable lipids distributions, yielding better biodiesel quality and equilibrium between biomass and lipids accumulation. The selected micronutrient combination (with the selected N/P ratio) leads to augment the lipids content which is transformed into biodiesel. Biodiesel quality (determined by cetane number, iodine value, cloud point, oxidation stability, viscosity), is evaluated considering microalgae lipids and FAMEs produced by transesterification process. Fourier transform infrared (FTIR) corroborates the lipids distribution, which can be used to produce high quality biodiesel. Gas chromatography (GC) verifies the production of desirable methyl esters (C16 and C18).

Influence of salinity and nitrogen in dark on Dunaliella tertiolecta’s lipid and carbohydrate productivity

In this study, the effects of salt and nitrogen were investigated on lipid and carbohydrate production in marine microalgae Dunaliella tertiolecta. All the experiments were performed in dark. Low salinity (3.7 PSU) was most suitable for lipid content (39%) and lipid productivity (100.584 mg/L/d). The maximum carbohydrate content (59.4%) and carbohydrate productivity (65.23 mg/L/d) were achieved in the absince of salinity. During nitrogen ( NaNO3) stress, the lipid and carbohydrate contents increased to 38.4% and 53.14% from 28% and 45%, respectively. However, interestingly, the effect of nitrogen stress on lipid and carbohydrate productivities was not significant.

Model Matematika Kendali Optimal Intensitas Cahaya Dan Nutrisi Pada Pertumbuhan Mikroalga Dengan Menggunakan Metode Pontryagin

Microalgae are the most primitive plant-sized cellular organisms commonly known as phytoplankton. The habitat of its life is in waters or humid places. This organism is a primary producer of water that has any capability to photosynthesis like any other high-level plants. This study examines mathematically the optimal control of light intensity and liquid waste nutrition in microalgae growth. Growth liter is done by setting the intensity of light in the process of glucose formation and nutrition tofu liquid waste, tapioca, industry, and households as the additional nutrients of microalgae. The Pontryagin maximun principles is used to determine the optimal control solution. The solution is solved from the state and co-state equation that stationery evaluated using the indexed performance maks 𝐽[𝑢1 + 𝑢2 ] = ∫ 𝐺(𝑡) − 𝑡𝑓 𝑡0 𝑆(𝑡) − 1 2 𝑢1 (𝑡) 2 − 1 2 𝑢2 (𝑡) 2𝑑𝑡 with the stationer condition that gives the optimal control 𝑢1 ∗ = 𝛾2𝛼2𝑄𝐵 and 𝑢2 ∗ = −𝛾5𝜌1𝑆. The results shows that before the optimal control of light intensity and nutrition of liquid waste is applied, the concentration of microalgae biomass becomes 5.915 g / liter on the 20th day stayed at the 105th day. The lipid quota with an initial value of 0.6 g/liter will decrease to 0.2 g / liter at 4th day which is the equilibrium point. Optimal control of the regulation of light intensity of 2-9 klux and liquid waste nutrition provided a significant increase in the amount of microalgae biomass and lipid quota, with the regulation of light intensity of 2- 9 klux and tofu liquid waste nutrition which gave the largest increase in the amount of microalgae biomass and lipid quota

Dewatering of Nannochloropsis Sp Via Forward Osmosis: A Molecular Dynamics Study

Microalgal biomass is an essential source of renewable energy and the production of high-valued bio-products. Nannochloropsis sp. is a marine microalgae which contains high valued bio-compounds. However, it contains elevated moisture which hinders extraction of important significant bio-compounds. A dewatering process of Nannochloropsis sp via forward osmosis is proposed. The objective of the study is to evaluate the effect of salinity on the lipid membrane of microalgae Nannochloropsis sp by inducing osmotic pressure difference. A microalgae lipid membrane model was developed using molecular dynamics and evaluated through relative structural changes and water dynamics by varying the salinity. The results revealed at elevated salinity, compression occurs to the lipid membrane based on the tail order and area per head groups. The dynamics of water molecules showed that water migration happens as early as 20 ns. Despite membrane compression, a total of 5 water molecules migrated within the 150 ns. This suggests that forward osmosis is a promising alternative for microalgal dewatering.

Lipid and biodiesel production by cultivation isolated strain Chlorella sorokiniana pa.91 and Chlorella vulgaris in dairy wastewater treatment plant effluents.

The present study provided a comparison of two species of microalgae growth in dairy wastewater treatment plant effluents. In optimum conditions their operation to biomass production, lipid accumulation and fatty acids methyl ester composition so as to biodiesel production is studied. For the first time, the not sterilized effluents of dairy wastewater treatment plant was used as the culture mediums of native microalgae, Chlorella sorokiniana strain pa.91, and another one Chlorella vulgaris. They were cultured under 5 light intensity levels so as to find optimum conditions to observed high biomass and lipid production. At the optimum light intensity the composition of fatty acids methyl ester in their lipids was analyzed by GC-MS. The light intensity of 7500 Lux was obtained as the optimum for both microalgae to produce high biomass. The biomass productivity of C. sorokiniana pa.91 and C. vulgaris in preliminary treated effluent at this light intensity was obtained 0.233 and 0.214gL-1day-1, respectively. This parameter in secondary treated effluent was achieved 0.185 and 0.166gL-1day-1, respectively. Moreover, the highest lipid content of their biomass was observed at the light intensity of 2500 Lux. At this light intensity and at the preliminary effluent the maximum lipid content of C. sorokiniana pa.91 and C. vulgaris was observed 31% and 34%, respectively and at the secondary one it was obtained 35% and 36.67%, respectively. Based on the results, the fatty acids composition in the lipids of microalgae C. sorokiniana pa.91 and C. vulgaris cultured in both effluents had the high amount of cetane number which is really useful for high quality biodiesel production. Also, the other valuable properties which produce the high quality biodiesel were the obtained amounts of CFPP and CP which shown a high performance potential biodiesel even at low temperatures. This feature was obtained, on the grounds that the unsaturated fatty acid was obtained more than saturated fatty acid. The nutrients-rich media of dairy wastewater effluents were applicable to growth both microalgae and useful biomass production, lipid accumulation and fatty acids profiling. Furthermore, the compounds of fatty acids had the best conditions to biodiesel production especially in cold weather areas.

Infrared spectroscopy as a tool to monitor interactions between nanoplastics and microalgae.

The unicellular photosynthetic organisms known as microalgae are becoming one of the most important models for aquatic system studies. Among them, Chlamydomonas reinhardtii is widely used as a bioindicator of pollution or of different changes in the environment. Numerous pollutants are present in aquatic environments, particularly plastics and nanoplastics. Physiological variations after an environmental change highlight variation in the macromolecular composition of microalgae (proteins, nucleic acids, lipids and carbohydrates). Recently, Fourier transform infrared vibrational spectroscopy has been described as a reliable tool, sensitive and allowing rapid measurement of macromolecular composition of microalgae. Coupled with preprocessing and principal component analysis, it is well adapted to monitoring the effect of environmental stress on biochemical composition. In this study, infrared spectroscopy, combined with multivariate analysis, has been tested first on known environmental stresses such as light intensity variation and nitrogen limitation. Then, this technique has been applied to monitor the interaction and potential impacts of polystyrene nanoparticles on microalgae. The results showed slight variations on protein and carbohydrates bands in the presence of nanoplastics, suggesting that their presence led to modifications in the biochemical composition of the microalgae. To confirm the interaction between microalgae and nanoplastics, visualization by confocal microscopy and cytotoxicity measurement has been carried out. Results showed that polystyrene nanoparticles seemed to adsorb on microalgae surface, leading to a loss of plasma membrane integrity. The resulting chemical modifications, even if moderate, could be detected by infrared spectroscopy' showing that this tool could be very helpful in the understanding of nanoparticle-microalgae interaction mechanisms.

A Comparative Review on Different Trophic Modes and Usable Carbon Sources for Microalgae Cultivation, Approaching to Optimize Lipid Production in Trade Scale

Microalgae are considered as an outstanding feedstock to produce high value lipid products like biodiesel and biomedicine. Reaching commercial maturity in this field is possible in the case of maximizing lipid yield and minimizing prime costs. In order to clarify the best features of carbon source (for microalgae cultivation) to reach optimum efficiency of biomass and lipid production, this paper reviews the merits and demerits of different trophic modes as well as type and concentration of carbon source. Furthermore carbon supplementation for large scale microalgae cultivation and lipid production is discussed as an economical point of view.

Improved lipid productivity in Nannochloropsis gaditana in nitrogen-replete conditions by selection of pale green mutants

BackgroundNannochloropsis gaditana is a photosynthetic unicellular microalgae considered one of the most interesting marine algae to produce biofuels and food additive due to its rapid growth rate and high lipid accumulation. Although microalgae are attractive platforms for solar energy bioconversion, the overall efficiency of photosynthesis is reduced due to the steep light gradient in photobioreactors. Moreover, accumulation of lipids in microalgae for biofuels production is usually induced in a two-phase cultivation process by nutrient starvation, with additional time and costs associated. In this work, a biotechnological approach was directed for the isolation of strains with improved light penetration in photobioreactor combined with increased lipids productivity.ResultsMutants of Nannochloropsis gaditana were obtained by chemical mutagenesis and screened for having both a reduced chlorophyll content per cell and increased affinity for Nile red, a fluorescent dye which binds to cellular lipid fraction. Accordingly, one mutant, called e8, was selected and characterized for having a 30% reduction of chlorophyll content per cell and an almost 80% increase of lipid productivity compared to WT in nutrient-replete conditions, with C16:0 and C18:0 fatty acids being more than doubled in the mutant. Whole-genome sequencing revealed mutations in 234 genes in e8 mutant among which there is a non-conservative mutation in the dgd1 synthase gene. This gene encodes for an enzyme involved in the biosynthesis of DGDG, one of the major lipids found in the thylakoid membrane and it is thus involved in chloroplast biogenesis. Lipid biosynthesis is strongly influenced by light availability in several microalgae species, including Nannochloropsis gaditana: reduced chlorophyll content per cell and more homogenous irradiance in photobioreactor is at the base for the increased lipid productivity observed in the e8 mutant.ConclusionsThe results herein obtained presents a promising strategy to produce algal biomass enriched in lipid fraction to be used for biofuel and biodiesel production in a single cultivation process, without the additional complexity of the nutrient starvation phase. Genome sequencing and identification of the mutations introduced in e8 mutant suggest possible genes responsible for the observed phenotypes, identifying putative target for future complementation and biotechnological application.

Fermentable sugar production from wet microalgae residual after biodiesel production assisted by radio frequency heating

In this study, the feasibility of comprehensive recovery of lipid and carbohydrate in wet microalgae Chlorella vulgaris was explored. First, four sets of enzyme combinations of α-Amylase, Amyloglucosidase and CTec2 were evaluated for hydrolysis efficiency on microalgae disrupted with radio frequency heating. Then, the most suitable combination was applied to raw microalgae and microalgae residual after biodiesel production, respectively, for saccharification. Adsorption kinetics of the optimized enzyme combination on the aforementioned three samples were determined and adsorption isotherm was analyzed by Freundlich equation. Morphology of microalgae was also investigated by scanning electron microscopy. A yield of reducing sugars in microalgae residual at 54.5% was obtained after 72 h saccharification. The results from enzyme adsorption kinetics, isotherm and SEM images were consistent with each other. This study demonstrated that the microalgae residual after biodiesel production could be used as carbohydrate feedstock for fermentable sugar production through simple enzymatic hydrolysis.

Treatment of fizzy drink factory wastewaters by microalgae and evaluation of algae oil for biofuel production

As a result of city, industrial and agricultural activities, a large amount of wastewater is generated every year. The wastewater generated must be treated. When the nitrogen concentration in wastewater reaches more than 1.9 mg / L, favorable environmental conditions are provided for the microalgae to multiply. In the presence of microalgae in wastewater treatment technologies, treatment has gained importance in recent years and has become a more environmentally-friendly alternative in treatment. Research on the availability of biomass as a source of raw materials in the production of alternative energy sources is increasingly important. In this paper, Chlorella variabilis microalgae growth, lipid productivity and nutrient removal in wastewater media were investigated. Before inoculation of microalgae, wastewater was centrifuged at 4000 rpm to remove the solid particles and was diluted with tap water in different ratios (0-40%). Wastewaters were inoculated with Chlorella variabilis in 250 mL open flasks in a 200 rpm shaking incubator for a month at 27 °C. After incubation maximum cell concentration (Xmax=1.03 gdw/L), growth rate (µmax=4.0 x10-3), doubling time (173 h) of the microalgae were reached in 40% diluted medium. Fat content (21%) and lipid productivity (6x10-3 g/L.d) were determined concurrently for 20% diluted media. It was determined that microalgae lipids were rich in oleic (C18:1 38%) and linolenic acid (C18:3 35%). The efficiency of COD (Chemical oxygen demand) and total phosphor removal in the presence of microalgae had been almost 60% and 77% respectively. According to the experimental results, the treatment of wastewater in the presence of microalgae is promising for future applications.

The effect of stress environment towards lipid accumulation in microalgae after harvesting

Microalgae have been recognized as a potential feedstock for biodiesel production due to their fast growth rate and ability to accumulate lipid within their cells. However, high lipid content and biomass productivity are usually difficult to achieve simultaneously. Instead of stressing the microalgae during cultivation stage, the stress environment such as nutrient starvation, salinity and light effect were introduced to Chlorella vulgaris after harvesting to study the effect on their lipid content. From the results attained, one day of nutrient starvation with 6.0 g/L of salinity stress under dark room condition had shown the highest lipid content of 38.8% (dry weight basis). The lipid content was recorded at 40.28% (dry weight basis) when the working volume was scaled up. In addition, the fatty acids identified in the extracted microalgae lipid were mainly consisted of linoleic, linolenic and palmitic acid, which were commonly found in corn oil, soybean oil and sunflower oil.