Lipid Profile Variables Research Articles

Elevated circulating HHIP levels in patients with metabolic syndrome

Abnormal fat accumulation can lead to metabolic syndrome (MetS), increasing the risk of diabetes and cardiovascular disease in MetS patients. Early identification of MetS risk is essential for effective disease prevention. Using bioinformatics methods, we sought biomarkers for MetS. After analyzing the GSE9624 and GSE15524 datasets, we identified three commonly differentially expressed genes: COX7A1, PRR12, and HHIP. Subsequently, we validated the expression of these DEGs using the GSE65540 dataset. Quantitative PCR and immunoblotting confirmed significantly elevated HHIP expression in the adipose tissue of HFD-fed and ob/ob mice. Furthermore, a population-based cohort study demonstrated that serum HHIP levels were significantly greater in MetS patients than in healthy controls and were correlated with all MetS components. Receiver operating characteristic (ROC) curve analysis confirmed the robust predictive capacity of HHIP levels for metabolic syndrome, with an area under the curve (AUC) of 0.72 (95% confidence interval: 0.68-0.78, P<0.001). Binary logistic regression showed that the serum HHIP concentration was significantly associated with MetS even after adjusting for anthropometric and lipid profile variables. In conclusion, our findings demonstrate that changes in HHIP expression are significantly associated with adverse MetS indicators, indicating that HHIP can serve as a new biomarker for the diagnosis of MetS.

Exploratory Metabolomics and Lipidomics Profiling Contributes to Understanding How Curcumin Improves Quality of Goat Semen Stored at 16 °C in Tropical Areas.

Reactive oxygen species (ROS) exert a vital role in sperm quality during semen preservation, where excessive ROS leads to oxidative damage and undermines sperm integrity. Curcumin, a botanical extract, is capable of neutralizing ROS and enhancing the activity of antioxidant enzymes. This study was aimed at evaluating the effects of curcumin on sperm viability, acrosome integrity, and antioxidant levels, as well as metabolomic and lipidomic profiles. The results demonstrated that curcumin at 25 µmol/L significantly enhanced sperm motility, plasma membrane, and acrosome integrity, elevated the levels of antioxidant enzymes (T-AOC, CAT, SOD), and decreased ROS production (p < 0.05). Metabolomic analysis identified 93 distinct metabolites that showed significant differences between the control and curcumin-treated groups. KEGG pathways emphasized the participation of these metabolites in key metabolic processes such as the citric acid cycle, cholesterol metabolism, and fatty acid metabolism. Curcumin treatment brought about notable variations in lipid profiles, including increased levels of phosphatidylcholine, acylcarnitine, and triglyceride over the storage time, suggesting enhanced lipid anabolic activity. Overall, the supplementation of curcumin at 25 µmol/L effectively mitigates oxidative stress and prolongs the viability of semen storage at 16 °C by modulating specific metabolic and lipid profiles.

Lipid composition of coral propagules and reproductive material in coral restoration nurseries

Coral restoration efforts have rapidly increased worldwide, including the development of several programmes on the Great Barrier Reef (GBR) in recent years. While many restoration programmes utilise in-water nurseries to accelerate coral biomass yields, the impact of nursery environments on propagule quality has not been examined despite the importance of coral fitness for ensuring resistant populations. Here, we investigated two fitness indicators (lipid diversity and tissue protein abundance) of Acropora millepora adults and eggs grown on coral nurseries versus native reef on the GBR, with adults assessed at two sites (Blue Lagoon and Rayban) and eggs assessed at one site (Blue Lagoon). Lipid profiles of adult colonies varied by site and origin (nursery versus wild reef), with adult nursery corals exhibiting an elevated relative abundance of storage lipids (diacylglycerols and triacylglycerols) and lipid classes responsible for regulating membrane structure (phosphatidylcholines and sterol esters), while wild corals were characterised by a greater relative abundance of fatty acids and classes involved in immunoregulation. Comparing eggs from different origins, nursery offspring were richer in energy-storing triacylglycerols, as well as ceramides and phosphatidylcholines essential for membrane structure, while wild eggs had a greater relative abundance of wax ester species also important for energy storage. No differences were found in total protein abundance (adult or eggs) or egg physical characteristics (count and size) between nursery and wild origins. Variations in lipid profiles are consistent with differences in environmental conditions between reef sites and origin (nursery versus wild), highlighting the need to consider site selection and propagation conditions when planning restoration projects. Importantly, these findings demonstrate that the lipid classes with the highest relative abundance in A. millepora nursery and wild eggs differed from those in adults from the same origin, suggesting that propagation origin is more important for driving lipid profiles in coral eggs compared to parental effects.

Long-Term Evaluation of Lipid Profile Changes in Olympic Athletes.

Dyslipidemia is a major contributor to the development of atherosclerotic cardiovascular disease. Despite high level of physical activity, athletes are not immune from dyslipidemia, but longitudinal data on the variation of lipids are currently lacking. We sought to assess lipid profile changes over time in Olympic athletes practicing different sports disciplines (power, skills, endurance, and mixed). We enrolled 957 consecutive athletes evaluated from London 2012 to Beijing 2022 Olympic Games. Dyslipidemia was defined as low-density lipoprotein (LDL) ≥115mg/dl, high-density lipoprotein (HDL) <40mg/dl for males, or HDL <50mg/dl for females. Hypertriglyceridemia was defined as triglycerides >150mg/dl. At the follow-up, a variation of ±40mg/dl for LDL, ±6mg/dl for HDL, and ±50mg/dl for triglycerides was considered relevant. Athletes with follow-up <10months or taking lower lipid agents were excluded. Follow-up was completed in 717 athletes (74.9%), with a mean duration of 55.6months. Mean age was 27.2 ± 4.8years old, 54.6% were male (n = 392). Overall, 19.8% (n = 142) athletes were dyslipidemic at both blood tests, being older, practicing nonendurance sports, and predominantly male. In 69.3% (n = 129) of those with elevated LDL at t0, altered values were confirmed at follow-up, while the same occurred in 36.5% (n = 15) with hypo-HDL and 5.3% (n = 1) in those with elevated triglycerides. Weight and fat mass percentage modifications did not affect lipid profile variation. LDL hypercholesterolemia tends to persist over time especially among male, older, and nonendurance athletes. LDL hypercholesterolemia detection in athletes should prompt early preventive intervention to reduce the risk of future development of atherosclerotic disease.

The Number of Antiseizure Medications Taken and not the Lipid Profile was Associated with Seizure Control in Adult Patients with Epilepsy.

Previous studies show changes in lipid metabolism in epilepsy. The aim of this study was to investigate the association between lipid profile and clinical variables in adult patients with epilepsy (APE). Seventy-two APE participated in this pilot study at an outpatient neurology service. The lipid profile (total cholesterol, low-density lipoprotein (LDL) cholesterol, very-low-density lipoproteins (VLDL) cholesterol, high-density lipoprotein (HDL) cholesterol and triglycerides), age at disease onset, disease duration, seizures frequency, and the number of antiseizure medications (ASM) used were investigated. Data were analyzed using the Chi-square, Fisher, Mann-Whitney, Spearman coefficient, and logistic regression tests. There were significant differences in HDL (p = 0.0023) and total cholesterol (p = 0.0452) levels in connection with the number of ASM used. There was a significant difference in seizure control among the different numbers of ASM used (p = 0.0382). Higher HDL values were found in females (p = 0.0170). The logistic regression showed that only the number of ASM used was associated with seizure control (p = 0.0408; OR = 2.800; 95% CI = 1.044; 7.509). The number of ASM taken and not the lipid profile was associated with seizure control in APE.

A comprehensive foodomics analysis of rambutan seed oils: Focusing on the physicochemical parameters, lipid concomitants and lipid profiles

A foodomics approach was employed to systematically characterize and compare the quality parameters, antioxidant activity, minor-components, fatty acid composition, and lipid profiles of the seed oils from the three most popular rambutan varieties in China. The total lipid content ranged from 23.40 to 25.77 g/100 g. The fatty acids 9cC18:1 (39.84%–40.92%) and C20:0 (28.45%–30.23%) were identified as the dominant ones, which are uncommon among higher plants. All oil samples exhibited low AI and TI values. BR-7 exhibited the highest levels of squalene (21.48 mg/kg), cholesterol (144.43 mg/kg), and tocopherol (17.42 mg/kg), and the lowest levels of polyphenols (24.21 mg GAE/kg). Additionally, a total of 807 lipid species were identified, with TAG, DGTS, and PE being the predominant ones. Multivariate statistical analyses revealed significant variations in lipid profiles among the varieties, particularly in glycerophospholipids and sphingolipids. Fifty-seven distinct lipids were identified as potential markers for distinguishing between rambutan varieties. Furthermore, a hypothetical scenario was developed by linking relevant lipid metabolism pathways. These findings establish a theoretical framework for comprehending rambutan seed oil in depth and unlocking its high-value potential.

Predicting dyslipidemia incidence: unleashing machine learning algorithms on Lifestyle Promotion Project data

BackgroundDyslipidemia, characterized by variations in plasma lipid profiles, poses a global health threat linked to millions of deaths annually.ObjectivesThis study focuses on predicting dyslipidemia incidence using machine learning methods, addressing the crucial need for early identification and intervention.MethodsThe dataset, derived from the Lifestyle Promotion Project (LPP) in East Azerbaijan Province, Iran, undergoes a comprehensive preprocessing, merging, and null handling process. Target selection involves five distinct dyslipidemia-related variables. Normalization techniques and three feature selection algorithms are applied to enhance predictive modeling.ResultThe study results underscore the potential of different machine learning algorithms, specifically multi-layer perceptron neural network (MLP), in reaching higher performance metrics such as accuracy, F1 score, sensitivity and specificity, among other machine learning methods. Among other algorithms, Random Forest also showed remarkable accuracies and outperformed K-Nearest Neighbors (KNN) in metrics like precision, recall, and F1 score. The study’s emphasis on feature selection detected meaningful patterns among five target variables related to dyslipidemia, indicating fundamental shared unities among dyslipidemia-related factors. Features such as waist circumference, serum vitamin D, blood pressure, sex, age, diabetes, and physical activity related to dyslipidemia.ConclusionThese results cooperatively highlight the complex nature of dyslipidemia and its connections with numerous factors, strengthening the importance of applying machine learning methods to understand and predict its incidence precisely.

Association between exposure to plasma mixture of essential and toxic elements and the lipid profile in institutionalized older adults

BackgroundOlder adults have a progressive deficiency in the ability to detoxify chemical elements and are susceptible to dyslipidemia and changes in glycemic control. The objective was to evaluate the association of the mixture of essential and toxic elements in the plasma of institutionalized older adults and test the associations with lipid profile variables and glycemic control. MethodsData were obtained from 149 Brazilian older adults aged ≥60 living in nursing homes (NH) in Natal, Brazil. The concentrations of sixteen chemical elements in plasma and lipid profile parameters and glycemic control of 149 institutionalized older adults were measured. Bayesian kernel machine regression was used to estimate the associations of the mixture of chemical elements with total cholesterol (TC), high-density lipoprotein (HDL-c), low-density lipoprotein (LDL-c), triglycerides (TG), fasting glucose, and glycated hemoglobin. ResultsNon-linear responses to exposure were observed for iron (Fe) about TC, LDL-c, and TG, and for barium (Ba) and copper (Cu) about TG. The concentration of the mixture of chemical elements below the 35th percentile was associated with a decrease in TC. Fe was the main element in the effect of the mixture associated with TC. ConclusionsThe lower concentrations of the mixture of chemical elements in plasma had a protective effect on the increase in TC, with Fe being the main element. Considering the results, the levels of essential and toxic elements in the plasma of older adults require extensive screening, mainly to prevent dyslipidemia and monitor clinical interventions.

Mapping the APOE structurally on missense variants in elderly Brazilians

Cardiovascular diseases (CVDs) pose a significant global health threat, with familial hypercholesterolemia (FH) being a key genetic contributor. The apolipoprotein E (APOE) gene plays a vital role in lipid metabolism, and its variants are associated with CVD risk. This study explores prevalent APOE variants (p.R163C, p.R176C, p.R246C and p.V254E) using genetic and structural analyses. The research, initiated by identifying high-frequency APOE variants through the ABraOM database, utilizes homology modeling and molecular dynamics (MD) simulations to understand the structural consequences. The major lipid-binding region, a critical domain for lipid metabolism, was a focal point. Structural dynamics, including principal component analyses and domain movement analyses, highlighted distinct patterns in APOE variants compared to the wild type (WT). Results revealed significant differences in the structural behavior of variants, particularly in the Major lipid-binding region. The identification of an ‘elbow’ structure with two states (Elbow I and Elbow II) provided insights into conformational changes. Notably, variants exhibited unique patterns in hydrogen bonding (hb) and hydrophobic interactions, indicating potential functional consequences. The study further associated APOE variants with clinical outcomes, including cognitive impairment and cholesterol levels. Specific variants demonstrated correlations with cognitive decline and variations in lipid profiles, emphasizing their relevance to cardiovascular and neurobiological health. In conclusion, this integrated approach enhances our understanding of APOE variants, shedding light on their role in lipid metabolism and cardiovascular health. The identified structural ‘elbows’ and their association with clinical outcomes offer a nuanced perspective, guiding future research toward targeted interventions for diseases linked to lipid metabolism and neurobiology. Communicated by Ramaswamy H. Sarma

Correlation of obstructive sleep apnea severity and lipid profile level

Obstructive sleep apnea (OSA) is accompanied by different metabolic disorders and is linked with a heightened incidence of cardiovascular diseases and death. The information actively engaged in relating obstructive sleep apnea and dyslipidemia is quite uncertain.This research intended to determine the correlation between obstructive sleep apnea severeness and lipid profile abnormalities. Our study involved 120 cases diagnosed with OSA, who came to the chest department: at Benha University Hospital from September 2021 to December 2022. Included patients indicated for polysomnography because of suspected sleep-breathing disorders. Full history and clinical examination, and full polysomnography and blood samples for lipid profile, were done for all the cases.Results A total of 120 patients were involved in the study. Most cases were severe OSA (70%). The mean age in severe obstructive sleep apnea was 44.04 years with SD 9.49, Males were 92.9% in severe OSA, and the mean BMI in severe OSA was 36.63 with SD 7.79. LDL was positively correlated with AHI and DI (significantly); triglycerides were positively correlated with AHI, DI, and min oxygen saturation (significantly); and HDL was negatively correlated with AHI and DI significantly. Among lipid profile variables, LDL was the most predictor for AHI severity.Conclusion Lipid abnormalities are associated with OSA severity. Patients diagnosed with obstructive sleep apnea should be screened for lipid profile to correct any abnormality, hoping to prevent their harmful effects.

CETP gene polymorphisms and haplotypes are explanatory variables for HDL cholesterol level in sickle cell disease.

Variations in lipid profile have been observed in sickle cell disease (SCD) and understanding their relationship with disease severity is crucial. This study aimed to investigate the association of polymorphisms of the CETP gene and laboratory markers of disease severity with lipid profile in a pediatric population with SCD. Biochemical and anthropometric analyses and CETP and alpha-thalassemia genotyping were performed. The study included 133 children and adolescents with sickle cell anemia (SCA) or hemoglobin SC disease (SCC), in steady-state. The SCA and no hydroxyurea (no HU) groups had higher values of ApoB, total cholesterol, low-density lipoprotein cholesterol (LDL-C), and non-high-density lipoprotein cholesterol (non-HDL-C) compared to the SCC and HU groups. However, there were no significant differences in ApoA1 and HDL-C levels between the groups based on genotype. Furthermore, the groups with altered levels of ApoA1, HDL-C, and the triglyceride/HDL ratio exhibited lower hemoglobin (Hb) levels and higher white blood cell counts. Hb level was associated to HDL-C levels. Analysis of CETP gene variants showed that the minor alleles of rs3764261 (C>A), rs247616 (C>T), and rs183130 (C>T), as well as the TTA haplotype, are explanatory variables for HDL-C levels. These findings suggested that dyslipidemia in SCD, specifically related to HDL-C levels, may be influenced by individual genetic background. Additionally, further investigation is needed to determine if clinical manifestations are impacted by CETP gene variants.

Short-Course High-Intensity Statin Treatment during Admission for Myocardial Infarction and LDL-Cholesterol Reduction-Impact on Tailored Lipid-Lowering Therapy at Discharge.

We hypothesized that a short-course high-intensity statin treatment during admission for myocardial infarction (MI) could rapidly reduce LDL-C and thus impact the choice of lipid-lowering therapy (LLT) at discharge. Our cohort comprised 133 MI patients (62.71 ± 11.3 years, 82% male) treated with atorvastatin 80 mg o.d. during admission. Basal LDL-C levels before admission were analyzed. We compared lipid profile variables before and during admission, and LLT at discharge was registered. Achieved theoretical LDL-C levels were estimated using LDL-C during admission and basal LDL-C as references and compared to LDL-C on first blood sample 4-6 weeks after discharge. A significant reduction in cholesterol from basal levels was noted during admission, including total cholesterol, triglycerides, HDL-C, non-HDL-C, and LDL-C (-39.23 ± 34.89 mg/dL, p < 0.001). LDL-C levels were reduced by 30% in days 1-2 and 40-45% in subsequent days (R2 0.766, p < 0.001). Using LDL-C during admission as a reference, most patients (88.7%) would theoretically achieve an LDL-C < 55 mg/dL with discharge LLT. However, if basal LDL-C levels were considered as a reference, only a small proportion of patients (30.1%) would achieve this lipid target, aligned with the proportion of patients with LDL-C < 55 mg/dL 4-6 weeks after discharge (36.8%). We conclude that statin treatment during admission for MI can induce a significant reduction in LDL-C and LLT at discharge is usually prescribed using LDL-C during admission as the reference, which leads to insufficient LDL-C reduction after discharge. Basal LDL-C before admission should be considered as the reference value for tailored LLT prescription.

Effects of hormonal contraceptives on lipid profile among women attending family planning unit in Goba Town Public Health Facilities, Bale, Southeast Ethiopia: a comparative cross-sectional study

BackgroundHormonal contraceptives are a widely used contraceptive method for the prevention of pregnancy in women. It is associated with change in lipid profile which results in congestive heart failure, coronary heart disease, angina, deep vein thrombosis and stroke which are the major cause of premature death. We aim to investigate the effects of hormonal contraceptive use on lipid profile among women attending family planning unit in Goba Town Public Health Facilities.MethodsA comparative cross-sectional study design was conducted on 93 hormonal contraceptive users and 93 non-users’ women in Goba Town Public Health Facilities from September to November, 2022. Blood samples for the estimation of TC, TG, HDL-c and LDL-c levels were collected. Student's independent t-test was used to compare the results of lipid profiles. One-way ANOVA was used to identify the variation of lipid profile between progestin only pills, DMPA and implant users. Simple linear regression was used to determine the change in lipid profiles in relation to the duration of hormonal contraceptive use. P-value less than 0.05 was considered as statistically significant.ResultThe mean serum level of TC, TG and LDL-c was significantly increases in hormonal contraceptive users in comparison with non-users. The mean serum level TC, TG, LDL-c and HDL-c was significantly different between DMPA, implanon and POP users. The mean serum level of TC, TG and LDL-c in implanon users was lower than DMPA and POP users. As the duration of DMPA and POP use increases, the serum level of TC, TG and LDL-c were significantly increased. But, the serum level of HDL-c was significantly decreased. LDL-c was significantly increased with the duration of implanon use.ConclusionThe mean serum level of TC, TG and LDL-c were significantly increased among hormonal contraceptive users. The mean serum level of lipid profile was significantly different between DMPA, implanon and POP users. The serum level of TC, TG, LDL-c were directly proportional to the duration of DMPA and POP use. Routine evaluation of lipid profiles is advisable before and after initiation of hormonal contraceptives.

Lipid Profile of Youth Sport Climbers: A Preliminary Investigation

Abstract Introduction. Lipid profile is one of the main determinants of cardiovascular health status. Moderate and vigorous physical activity can positively influence lipid profile, and one such activity is sport climbing. The main aim of this study was to determine the lipid profile and to investigate the influence of training experience on the lipid profile of youth climbers. Material and Methods. This research included 12 youth climbers aged 15.33 ± 1.30 years (6 males, 6 females), who were tested on anthropo-metric indices and lipid profile (total cholesterol, high-density lipoproteins, low-density lipoproteins, triglycerides) using the point-of-care testing. Variables of lipid profile were correlated with years of climbing experience by Spearman’s rank correlation coefficients, while the differences between females and males were checked by the Mann-Whitney U test. Results. Youth climbers had acceptable levels of all measured lipid parameters. Training experience was significantly positively correlated with body mass index (R = 0.76, p &lt; 0.01) and significantly negatively correlated with cholesterol (R = -0.80, p &lt; 0.01). Conclusions. Results support that climbing as a vigorous and enjoyable physical activity might be advertised as a proxy for reaching recommended amounts of physical activity among adolescents, which is associated with improved lipid profile. However, this is only a preliminary investigation advocating that determinants of health status related to climbing practice should be further investigated.

Correlation of Atherogenic Index of Plasma and Atherogenic Coefficient with Cardiovascular Disease Risk assessed by ASCVD Risk Calculator

Introduction: Cardiovascular disease (CVD) is the leading cause of approximately one-third deaths worldwide. Absolute lipid parameters are inadequate in predicting CVD risk and several lipid indices have been introduced namely “Atherogenic index of plasma” (AIP) and “Atherogenic coefficient” (AC). CVD risk of a patient for the upcoming 10 years can be calculated using atherosclerotic cardiovascular disease (ASCVD) risk calculator. Though, this method currently accepts as the gold standard, certain drawbacks have been reported. Therefore, the aim of this study was to investigate the correlation of AIP and AC with CVD risk estimation for the upcoming 10 years calculated by ASCVD risk calculator. Methods: Hundred and fifty-three patients were recruited for the study. Socio-demographic data were collected through interviewer-based questionnaire. Total cholesterol, HDL cholesterol, LDL cholesterol and triglycerides values were obtained from the lipid profile test results of the study participants. AIP and AC were calculated using formulas while 10-year ASCVD risk was calculated by ASCVD risk calculator. Results: There was no significant correlation between AIP and 10-year ASCVD risk (p&gt;0.05) and AC and 10-year ASCVD risk (p&gt;0.05), but a significant correlation was observed between AIP and AC (Pearson’s correlation r=0.425, p&lt;0.05). Conclusions: CVD risk of patients should be assessed routinely especially as it is overlooked in most patients presenting normal lipid profile variables, hence the actual risk remains silent unless it is properly investigated using laboratory investigations

Oral magnesium supplementation does not affect insulin sensitivity in people with insulin-treated type 2 diabetes and a low serum magnesium: a randomised controlled trial

Aims/hypothesisHypomagnesaemia has been associated with insulin resistance and an increased risk of type 2 diabetes. Whether magnesium supplementation improves insulin sensitivity in people with type 2 diabetes and a low serum magnesium level is unknown.MethodsUsing a randomised, double-blind (both participants and investigators were blinded to the participants’ treatment sequences), placebo-controlled, crossover study design, we compared the effect of oral magnesium supplementation (15 mmol/day) for 6 weeks with that of matched placebo in individuals with insulin-treated type 2 diabetes (age ≥18 years, BMI 18–40 kg/m2, HbA1c <100 mmol/mol [11.3%], serum magnesium ≤0.79 mmol/l). Participants were recruited from the outpatient clinic and through advertisements. Randomisation to a treatment sequence order was done using a randomisation list. We used block randomisation and the two possible treatment sequences were evenly distributed among the trial population. The primary outcome was the mean glucose infusion rate during the final 30 min of a hyperinsulinaemic–euglycaemic clamp (i.e. M value). Secondary outcomes included variables of glucose control, insulin need, BP, lipid profile and hypomagnesaemia-related symptoms during follow-up.ResultsWe recruited 14 participants (50% women, 100% White, mean ± SD age 67±6 years, BMI 31±5 kg/m2, HbA1c 58±9 mmol/mol [7.4±0.9%]) with insulin-treated type 2 diabetes. Magnesium supplementation increased both mean ± SEM serum magnesium level (0.75±0.02 vs 0.70±0.02 mmol/l, p=0.016) and urinary magnesium excretion (magnesium/creatinine ratio, 0.23±0.02 vs 0.15±0.02, p=0.005), as compared with placebo. The M value of the glucose clamp did not differ between the magnesium and placebo study arms (4.6±0.5 vs 4.4±0.6 mg kg−1 min−1, p=0.108). During the 6 weeks of treatment, continuous glucose monitoring outcomes, HbA1c, insulin dose, lipid profile and BP also did not differ, except for a lower HDL-cholesterol concentration after magnesium compared with placebo (1.14±0.08 vs 1.20±0.09 mmol/l, p=0.026). Symptoms potentially related to hypomagnesaemia were similar for both treatment arms.Conclusions/interpretationDespite an albeit modest increase in serum magnesium concentration, oral magnesium supplementation does not improve insulin sensitivity in people with insulin-treated type 2 diabetes and low magnesium levels.Trial registrationEudraCT number 2021-001243-27.FundingThis study was supported by a grant from the Dutch Diabetes Research Foundation (2017–81–014).Graphical

Variations in the Relative Abundance of Gut Bacteria Correlate with Lipid Profiles in Healthy Adults.

The gut microbiome is a versatile system regulating numerous aspects of host metabolism. Among other traits, variations in the composition of gut microbial communities are related to blood lipid patterns and hyperlipidaemia, yet inconsistent association patterns exist. This study aims to assess the relationships between the composition of the gut microbiome and variations in lipid profiles among healthy adults. This study used data and samples from 23 adult participants of a previously conducted dietary intervention study. Circulating lipid measurements and whole-metagenome sequences of the gut microbiome were derived from 180 blood and faecal samples collected from eight visits distributed across an 11-week study. Lipid-related variables explained approximately 4.5% of the variation in gut microbiome compositions, with higher effects observed for total cholesterol and high-density lipoproteins. Species from the genera Odoribacter, Anaerostipes, and Parabacteroides correlated with increased serum lipid levels, whereas probiotic species like Akkermansia muciniphila were more abundant among participants with healthier blood lipid profiles. An inverse correlation with serum cholesterol was also observed for Massilistercora timonensis, a player in regulating lipid turnover. The observed correlation patterns add to the growing evidence supporting the role of the gut microbiome as an essential regulator of host lipid metabolism.

Serum Omentin-1 Levels in Obese Adolescents with Vitamin D Deficiency: A Prospective Cross-sectional Study

Background: Vitamin D deficiency is common in obese adolescents. It modulates the release of omentin 1 from adipose tissue. We believe that both vitamin D and omentin 1 affect each other in adipose tissue via inflammation. Objectives: This study aimed to examine serum omentin-1 levels in obese adolescents with vitamin D (Vit D) deficiency. Methods: In this cross-sectional prospective study, 83 obese adolescents were included. Serum 25-hydroxy vitamin D (25(OH)D) concentrations, fasting glucose, and lipid profiles of obese adolescents were studied. At the same time, blood was drawn into a separate tube to study the omentin 1 level. Of the 83 obese cases, 45 with 25(OH)D concentrations below 20 ng/mL were considered as the study group, and 38 with 25(OH)D concentrations ≥ 20 ng/mL as the control group. Serum omentin-1 levels were evaluated and compared. Results: The average 25(OH)D value in the study and control groups was 17.14 ± 2.22 ng/mL and 45.29 ± 24.98 ng/mL, respectively. The average omentin-1 concentration of the control group was 262.5 ± 136.31 ng/mL, and the mean omentin-1 level of the study group was 113.23 ± 15.98 ng/mL. The mean omentin-1 concentrations of the study group were significantly lower compared to the control group (P = 0.0001). There was a significant and positive correlation between omentin-1 and 25(OH)D (r = 0.988, P = 0.0001). In univariate tests, linear regression analysis was carried out with 25(OH)D and omentin-1, and 25(OH)D displayed a significant positive correlation (P = 0.0001). The optimal cut-off point for the serum omentin-1 concentration was 135.01 ng/mL. No significant correlation was determined between omentin-1 and body mass index, lipid profile, glucose, and insulin variables (P &gt; 0.05). Conclusions: We showed significantly low concentrations of serum omentin-1 in obese adolescents with vitamin D deficiency. Serum omentin-1 can be employed as a biomarker in obese adolescents with vitamin D deficiency.

Exploring PCSK9 Genetic Impact on Lipoprotein(a) via Dual Approaches: Association and Mendelian Randomization.

Previous investigations have suggested an association between the PCSK9 common polymorphism E670G and Lipoprotein(a) (Lp(a)) levels, as well as a link between plasma PCSK9 levels and Lp(a) concentrations. However, the causal relationship between plasma PCSK9 and Lp(a) levels remains uncertain. In this study, we explored the association between PCSK9 E670G polymorphism and Lp(a) levels in 614 healthy Taiwanese individuals. Employing a two-sample Mendelian randomization (MR) analysis using openly accessible PCSK9 and Lp(a) summary statistics from the genome-wide association studies (GWAS) and UK Biobank, we aimed to determine if a causal link exists between plasma PCSK9 levels and Lp(a) concentrations. Our findings reveal that the E670G G allele is independently associated with a decreased likelihood of developing elevated Lp(a) levels. This association persists even after adjusting for common cardiovascular risk factors and irrespective of lipid profile variations. The MR analysis, utilizing six PCSK9 GWAS-associated variants as instrumental variables to predict plasma PCSK9 levels, provides compelling evidence of a causal relationship between plasma PCSK9 levels and Lp(a) concentration. In conclusion, our study not only replicates the association between the PCSK9 E670G polymorphism and Lp(a) levels but also confirms a causative relationship between PCSK9 levels and Lp(a) concentrations through MR analysis.

Evaluation of Lipid Profile Changes In Patients With Inflammatory Bowel Disease: A Single Center Experience

Introduction: Inflammatory Bowel Disease (IBD) encompasses chronic inflammatory disorders of the gastrointestinal tract, including Crohn's disease and ulcerative colitis. While initially considered a localized disorder, IBD has emerged as a systemic disease with a growing recognition of its potential impact on lipid metabolism and cardiovascular health. This study aims to comprehensively investigate lipid profiles and dyslipidemia prevalence among IBD patients, shedding light on potential associations with disease characteristics. Materials and Methods: A single-center cross-sectional observational study was conducted on 707 IBD patients between December 2007 and February 2021. Inclusion criteria comprised confirmed IBD diagnosis, age 18-65, and informed consent. Demographic data, medical history, anthropometric measurements, and fasting blood samples for lipid profile analysis were collected. Lipid profiles included total cholesterol, high-density lipoprotein cholesterol (HDL-C), lowdensity lipoprotein cholesterol (LDL-C), and triglycerides. Statistical analyses, including t-tests, chi-square tests, and multivariate regression, explored associations. Results: This study reveals a high prevalence of dyslipidemia in IBD patients (58%), surpassing the general population. Notably, male IBD patients displayed significantly higher levels of total cholesterol, LDL-C, and triglycerides than their counterparts in the general male population. Differences in HDL-C levels were also observed. There were no significant variations in lipid profiles between Crohn's disease and ulcerative colitis patients within the IBD subgroup. Conclusion: The findings underscore the importance of regular lipid profile assessments in clinical management, particularly for male IBD patients. The significantly elevated prevalence of dyslipidemia in this population implies a potential influence of IBD on lipid metabolism and cardiovascular risk. Further research is needed to elucidate mechanisms and potential therapeutic interventions. Addressing dyslipidemia among IBD patients holds promise for reducing cardiovascular risk and enhancing overall health outcomes in this complex patient group.