Lipid Production Research Articles

Advanced treatment of antibiotic-polluted wastewater by a consortium composed of bacteria and mixed cyanobacteria

This study constructed a cyanobacteria-bacteria consortium using a mixture of non-toxic cyanobacteria (Synechococcus sp. and Chroococcus sp.) immobilized in calcium alginate and native bacteria in wastewater. The consortium was used for the advanced treatment of sulfamethoxazole-polluted wastewater and the production of cyanobacterial lipid. Mixed cyanobacteria increased the abundances of denitrifying bacteria and phosphorus-accumulating bacteria as well as stimulated various functional enzymes in the wastewater bacterial community, which efficiently removed 70.01–71.86% of TN, 91.45–97.04% of TP and 70.72–76.85% of COD from the wastewater. The removal efficiency of 55.29–69.90% for sulfamethoxazole was mainly attributed to the upregulation of genes encoding oxidases, reductases, oxidoreductases and transferases in two cyanobacterial species as well as the increased abundances of Stenotrophomonas, Sediminibacterium, Arenimonas, Novosphingobium, Flavobacterium and Hydrogenophaga in wastewater bacterial community. Transcriptomic responses proved that mixed cyanobacteria presented an elevated lipid productivity of 33.90 mg/L/day as an adaptive stress response to sulfamethoxazole. Sediminibacterium, Flavobacterium and Exiguobacterium in the wastewater bacterial community may also promote cyanobacterial lipid synthesis through symbiosis. Results of this study proved that the mixed cyanobacteria-bacteria consortium was a promising approach for advanced wastewater treatment coupled to cyanobacterial lipid production.

Microalgae cultivation with recycled harvesting water achieved economic and sustainable production of biomass and lipid: Feasibility assessment and inhibitory factors analysis

This study was conducted to achieve economic and sustainable production of biomass and lipids from Chlorella sorokiniana by recirculating cultivation with recycled harvesting water, to identify the major inhibitory factors in recirculating culture, and to analyze accordingly economic benefits. The results showed that recirculating microalgae cultivation (RMC) could obtain 0.20–0.32 g/L biomass and lipid content increased by 23.1 %-38.5 %. Correlation analysis showed that the extracellular polysaccharide (PSext), chemical oxygen demand (COD) and chromaticity of recirculating water inhibited photosynthesis and induced oxidative stress, thus inhibiting the growth of C. sorokiniana. In addition, the economic benefits analysis found that circulating the medium twice could save about 30 % of production cost, which is the most economical RMC solution. In conclusion, this study verified the feasibility and economy of RMC, and provided a better understanding of inhibitory factors identification in culture.

Increasing lipid production in Chlamydomonas reinhardtii through genetic introduction for the overexpression of glyceraldehyde-3-phosphate dehydrogenase.

Microalgae, valued for their sustainability and CO2 fixation capabilities, are emerging as promising sources of biofuels and high-value compounds. This study aimed to boost lipid production in C. reinhardtii by overexpressing chloroplast glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a key enzyme in the Calvin cycle and glycolysis, under the control of a nitrogen-inducible NIT1 promoter, to positively impact overall carbon metabolism. The standout transformant, PNG#7, exhibited significantly increased lipid production under nitrogen starvation, with biomass rising by 44% and 76% on days 4 and 16, respectively. Fatty acid methyl ester (FAME) content in PNG#7 surged by 2.4-fold and 2.1-fold, notably surpassing the wild type (WT) in lipid productivity by 3.4 and 3.7 times on days 4 and 16, respectively. Transcriptome analysis revealed a tenfold increase in transgenic GAPDH expression and significant upregulation of genes involved in fatty acid and triacylglycerol synthesis, especially the gene encoding acyl-carrier protein gene (ACP, Cre13. g577100. t1.2). In contrast, genes related to cellulose synthesis were downregulated. Single Nucleotide Polymorphism (SNP)/Indel analysis indicated substantial DNA modifications, which likely contributed to the observed extensive transcriptomic and phenotypic changes. These findings suggest that overexpressing chloroplast GAPDH, coupled with genetic modifications, effectively enhances lipid synthesis in C. reinhardtii. This study not only underscores the potential of chloroplast GAPDH overexpression in microalgal lipid synthesis but also highlights the expansive potential of metabolic engineering in microalgae for biofuel production.

The effect of nanoparticle surface charge on freshwater algae growth, reproduction, and lipid production

Using functionalized carbon dots as models, positively charged nanoparticles were found to cause growth inhibition and aggregation in microalgae. Negative and near-neutral negative particles were associated with morphological changes.

Enhancing biomass and lipid production in marine microalga Dunaliella tertiolecta with physico-chemical modulation and using cheap fertilizer substrate

Enhancing biomass and lipid production in marine microalga Dunaliella tertiolecta with physico-chemical modulation and using cheap fertilizer substrate

Optimization of Bioenergy Production from Algae Using Bioreactor Design

Bioenergy production from algae has gained significant attention due to its potential as a sustainable and renewable energy source. This research investigates the optimization of bioenergy production by focusing on bioreactor design to enhance the efficiency of algal biomass growth and lipid extraction. The study involves analyzing key bioreactor parameters such as light intensity, nutrient concentration, pH levels, and carbon dioxide (CO₂) supply. Experimental results demonstrated a 25% increase in lipid production through optimized conditions, showing promising improvements in biofuel yield. The findings of this research provide valuable insights into the potential scalability of algae-based bioenergy systems.

The role of lipid oxidation pathway in reactive oxygen species-mediated cargo release from liposomes.

Reactive oxygen species (ROS)-mediated photooxidation is an efficient method for triggering a drug release from liposomes. In addition to the release of small molecules, it also allows the release of large macromolecules, making it a versatile tool for controlled drug delivery. However, the exact release mechanism of large macromolecules from ROS-sensitive liposomes is still unclear. There are no studies on the effect of lipid oxidation on the release of cargo molecules of different sizes. By using HPLC-HRMS method we analyzed the oxidation products of ROS-sensitive DOTAP lipid in phthalocyanine-loaded DOTAP:Cholesterol:DSPE-PEG liposomes after 630 nm light irradiation of different durations. Shorter illumination time (1-2 minutes) led to the formation of hydroperoxides and vic-alcohols predominantly. Longer 9-minute irradiation resulted already in aldehydes generation. Interestingly, the presence of epoxides/mono-hydroperoxides and vic-alcohols in a lipid bilayer ensured a high 90% release of small hydrophilic cargo molecules i.e. calcein, but not large (≥10 KDa) macromolecules. Oxidation till aldehydes was mandatory to deliver e.g. dextrans of 10-70 kDa with ca. 30% efficiency. Molecular dynamics simulations revealed that the formation of aldehydes is required to form pores or even fully disrupt the lipid membrane, while e.g. presence of hydroperoxides is enough to make the bilayer more permeable just for water and small molecules. This is an important finding that shed a light on the release mechanism of different cargo molecules from ROS-sensitive drug delivery systems.

To explore the pathogenesis and scientific connotation of non‐alcoholic fatty liver based on the theory of turbid toxin of traditional Chinese medicine

AbstractNon‐alcoholic fatty liver disease (NAFLD) is a metabolic liver disease characterized by the accumulation of excessive liver lipids without alcohol‐induced damage. It has emerged as a significant global health issue. A recent research has indicated that dysregulation of lipid decomposition, uptake, production, oxidation, and secretion causes alterations in various types of lipids, leading to organelle dysfunction and metabolic signaling pathway impairment. Scholars propose that turbid toxin is crucial in the pathogenesis of NAFLD. This article elucidates the meaning of turbid toxin in conjunction with the current literature and analyzes the modern pathophysiological mechanism underlying the development of NAFLD, including turbid toxic substance production, related organelle involvement, biological processes, and pathways, ultimately leading to disease outcomes. The relationship between traditional Chinese medicine and the turbid toxin is also explored to establish a foundation for further mechanistic research on NAFLD.

Oxidized Low-Density Lipoprotein: Preparation, Validation, and Use in Cell Models.

Lipoproteins in plasma are constituted by the least dense chylomicron, very-low-density lipoprotein (VLDL), low-density lipoprotein (LDL), and high-density lipoprotein (HDL) that can be separated using commercially available medium such as iodixanol. Iodixanol constitutes the self-generated density gradient to fractionate lipoproteins by rapid ultracentrifugation method, replacing time-consuming protocols. Filling the centrifuge tubes is technically easier and faster than layering salt gradients and is reproducible. The separated lipoproteins by this method are closest to the native state with 80 to 100% recovery possible. Low-density lipoprotein is the major carrier of cholesterol in systemic circulation. The plasma isolated LDL is purified to be used as native LDL and for the preparation of oxidized LDL (oxLDL). The oxLDL is characterized for its oxidation, by various methods based on assay of the lipid and protein oxidation products such as TBARS, conjugated diene formation, and by other methods such as agarose gel electrophoresis. Rapid isolation of LDL particles from human plasma is useful for lipid peroxidation studies, characterization of subclass for functional studies and clinical correlation especially in cardiovascular diseases apart from lipidomic, and proteomic studies. OxLDL preparations are done in vitro chiefly based on copper-induced oxidation; glucose and other prooxidants. Which are used for various studies using animal model and in vitro cell modelsespecially to understand macrophage-mediated atheroma formation, vascular endothelial cell dysfunction, cell signaling studies has scope for extensive researchin metabolic dysfunction of various cells.This chapter deals with one of the applications in the in vitro cell models using macrophage (THP-1 cell line) and human retinal pigment epithelial cell (ARPE-19 cell line) to study the oxLDL uptake using fluorescently labeled oxidized LDL (DiI-oxLDL).

The Regulatory Mechanism of Smilax China L. Saponins against Nonalcoholic Fatty Liver Is Revealed by Metabolomics and Transcriptomics.

This study was to investigate the effects of Smilax China L. saponins (SCS) on non-alcoholic fatty liver disease (NAFLD). Rats were fed a high-fat diet (HFD) for 8 weeks to induce NAFLD, followed by SCS treatment for 8 weeks. The effect of SCS on liver injury was observed by H&E staining and the regulative mechanism of SCS on lipid formation was exposed by detecting Oil red O, insulin resistance (IR), and fatty acids synthesis (FAS). Furthermore, transcriptomics and metabolomics were performed to analyze the potential targets. The experimental results indicated that SCS exerted a positive curative effect in alleviating HFD-induced overweight, hepatic injury, steatosis, and lipid formation and accumulation in rats, and the preliminary mechanism studies showed that SCS could alleviate IR, inhibit FAS expression, and reduce Acetyl-CoA levels. Besides, the integrative analysis of transcriptomics and metabolomics exposed the targets of SCS to regulate lipid production likely being the sphingolipid metabolism and glycerophospholipid metabolism pathways. This study demonstrates that SCS significantly ameliorates lipid metabolic disturbance in rats with NAFLD by relieving insulin resistance, inhibiting the FAS enzymes, and regulating the sphingolipid and glycerophospholipid metabolism pathways.

Влияние фиксированной комбинации алоглиптина и пиоглитазона на факторы риска возникновения сердечно-сосудистых заболеваний у пациентов сахарным диабетом 2 типа

Objective: to study the dynamics of carbohydrate and lipid metabolism and markers of visceral obesity (VO) in patients with type 2 diabetes mellitus (T2DM) and obesity when using a fixed combination of alogliptin and pioglitazone. Material and methods. 30 patients with T2DM and obesity were examined. Indicators of carbohydrate and lipid metabolism and markers of VO (conicity index, index of lipid products accumulation, waist circumference to height ratio) were assessed initially and over time 6 months after the start of using alogliptin 25 mg and pioglitazone 30 mg. Results. When taking alogliptin and pioglitazone, patients with T2DM and obesity showed a compensation of fasting and postprandial hyperglycemia, an effective reduction in glycated hemoglobin and insulin resistance of peripheral tissues, and favorable changes in lipid metabolism with a positive effect on the calculated VO indices. Conclusions. The combination of alogliptin and pioglitazone can be recommended as a reliable and safe treatment regimen for patients with T2DM and obesity with the ability to correct major cardiovascular risk factors.

Differences in phytoplankton population vulnerability in response to chemical activity of mixtures.

Hydrophobic organic contaminants (HOCs) affect phytoplankton at cellular to population levels, ultimately impacting communities and ecosystems. Baseline toxicants, such as some HOCs, predominantly partition to biological membranes and storage lipids. Predicting their toxic effects on phytoplankton populations therefore requires consideration beyond cell uptake and diffusion. Functional traits like lipid content and profile can offer insights into the diverse responses of phytoplankton populations exposed to HOCs. Our study investigated the vulnerability of five phytoplankton species populations to varying chemical activities of a mixture of polycyclic aromatic hydrocarbons (PAHs). Population vulnerability was assessed based on intrinsic sensitivities (toxicokinetic and toxicodynamic), and demography. Despite similar chemical activities in biota within the exposed algae, effects varied significantly. According to the chemical activity causing 50% of the growth inhibition (Ea50), we found that the diatom Phaeodactylum tricornutum (Ea50 = 0.203) was the least affected by the chemical exposure and was also a species with low lipid content. In contrast, Prymnesium parvum (Ea50 = 0.072) and Rhodomonas salina (Ea50 = 0.08), both with high lipid content and high diversity of fatty acids in non-exposed samples, were more vulnerable to the chemical mixture. Moreover, the species P. parvum, P. tricornutum, and Nannochloris sp., displayed increased lipid production, evidenced as 5-10% increase in lipid fluorescence, after exposure to the chemical mixture. This lipid increase has the potential to alter the intrinsic sensitivity of the populations because storage lipids facilitate membrane repair, reconstitution and may, in the short-term, dilute contaminants within cells. Our study integrated principles of thermodynamics through the assessment of membrane saturation (i.e. chemical activity), and a lipid trait-based assessment to elucidate the differences in population vulnerability among phytoplankton species exposed to HOC mixtures.

Metabolic engineering and genome editing strategies for enhanced lipid production in microalgae

Metabolic engineering and genome editing strategies for enhanced lipid production in microalgae

The Potential of Lignocellulosic Biomass Hydrolysates for Microbial Oil Production Using Yeasts and Microalgae

The use of food‐based biomass and arable land for bio‐oil and biofuel production could compromise global food security. Therefore, renewable and environmentally friendly oils for biofuels from oleaginous microorganisms such as yeasts and microalgae (heterotrophic and mixotrophic) are gaining interest within the scientific community. These microorganisms have shorter cultivation times and higher lipid productivity when compared to higher plants/food crops/autotrophic microorganisms. Despite many advantages, the high carbon requirements and production cost are limiting factors that hinder their deployment at a commercial scale. Lignocellulosic waste substrates are abundant and inexpensive materials that are rich in organic carbon in the form of cellulose and hemicellulose, which release bioavailable forms of sugars upon hydrolysis. Recent studies have shown the tremendous potential of the hydrolysates of these substrates to be utilized as carbon sources for biomass production and the accumulation of lipids in oleaginous hetero‐/mixotrophic microorganisms. Therefore, this review highlights the potential use of lignocellulosic biomass as a low‐cost carbon substrate for the cultivation of hetero‐/mixotrophic microalgae and yeast for microbial oil production for commercial applications. It also examines the current status, challenges, and future prospects for the utilization of lignocellulose biomass.

Growth and Lipid Production of Marine Microalgae Tetraselmis Chuii Cultured in Different Phosphorus Concentrations

Lipid produced in the marine microalgae, Tetraselmis chuii has the potential to be processed into biofuels which can replace the use of fossil fuels that contributes to the global warming on earth. The purpose of this study was to determine the effects of phosphorus on lipid production in Tetraselmis chuii. Therefore, Tetraselmis chuii was cultured under normal concentration of phosphorus in the Conway medium. The cell was harvested on the fourth day and then transferred and cultured under different concentrations of phosphorus (10g, 15g, 20g, 25g and 30g of NaH2 PO4 .2H2 O in 1 L Conway medium). The cell density and optical density analysis of the culture were analyzed daily whereas the biomass (dry weight) analysis and lipid extractions were carried out on every alternate day. Result of the study showed that the highest lipid production in Tetraselmis chuii was achieved on day 4 in the phosphorus sufficient culture medium (25 g/L of NaH2 PO4 .2H2 O). Under the deficiency of phosphorus in the culture medium especially when the growth reached the stationary phase, more lipids accumulated in all the treatments. Therefore, our findings illustrated that phosphorus was important in regulating the growth of Tetraselmis chuii and the lipid accumulation was higher when the growth of Tetraselmis chuii was limited by the depleted phosphorus. In addition, total lipid yields also increased with the presence of excess phosphorus in the culture medium.

Enhanced microalgal biomass and lipid production with simultaneous effective removal of Cd using algae-bacteria-activated carbon consortium added with organic carbon source

Recently, using microalgae to remediate heavy metal polluted water has been attained a huge attention. However, heavy metals are generally toxic to microalgae and consequently decrease biomass accumulation. To address this issue, the feasibility of adding exogenous glucose, employing algae-bacteria system and algae-bacteria-activated carbon consortium to enhance microalgae growth were evaluated. The result showed that Cd2+ removal efficiency was negatively correlated with microalgal specific growth rate. The exogenous glucose alleviated the heavy metal toxicity to algal cells and thus increased the microalgae growth rate. Among the different treatments, the algae-bacteria-activated carbon combination had the highest biomass concentration (1.15 g L−1) and lipid yield (334.97 mg L−1), which were respectively 3.03 times of biomass (0.38 g L−1) and 4.92 times of lipid yield (68.08 mg L−1) in the single microalgae treatment system. Additionally, this algae-bacteria-activated carbon consortium remained a high Cd2+ removal efficiency (91.61%). In all, the present study developed an approach that had a great potential in simultaneous heavy metal wastewater treatment and microalgal lipid production.

Exploring The Potential of Microalgae-Fungi Co-Cultivation for Sustainable Bioprocessing in Microalgae Biorefinery

Developing co-cultivation systems involving microalgae and fungi has shown promising potential for microalgae harvesting technology. As discussed in this review, the co-cultivation of microalgae and fungi has emerged as a novel approach for enhancing biomass and lipid production, wastewater treatment, biofuel production, and high-value products. However, despite being used for a few years, this technique is still in its early stages of development and has yet to be widely applied in the industry. The main challenges associated with co-cultivation include designing effective cultivation systems, managing nutrient requirements, selecting compatible strains, and implementing contamination control measures. In this study, bibliometric analysis was conducted (using the Web of Science database) to examine global trends and developments in microalgae-fungi co-cultivation research between 2014 and 2023, which aimed to identify the research progression, prominent contributors, and leading countries in the research field. The dataset comprised 682 articles, 242 reviews, 31 book chapters, and 22 conference papers. The results showed a rapid increment of publications with China as an active nation in this research area, followed by India, the USA, Italy, Spain, etc. As demonstrated in this study, the immense potential of co-cultivation techniques suggests further exploration, particularly in employing different microalgae species with exceptional characteristics in conjunction with non-pathogenic and edible fungi for profitable industrialization.

Nutrient sequestration and lipid production potential of Chlorella vulgaris under pharmaceutical wastewater treatment: experimental, optimization, and prediction modeling studies.

The efficient management and treatment of pharmaceutical industry wastewater (PIWW) have become a serious environmental issue due to its high toxicity. To overcome this problem, the present study deals with the phycoremediation of PIWW using Chlorella vulgaris microalga isolated from the Ganga River at Haridwar, India. For this, response surface methodology (RSM) and artificial neural network (ANN) tools were used to identify the best reduction of total phosphorus (TP) and total Kjeldahl's nitrogen (TKN) based pollutants along with the lipid production efficiency of C. vulgaris. Three different concentrations of pharmaceutical wastewater (0, 50, and 100%), operating temperatures (20, 25, and 30 °C), and light intensity (2000, 3000, and 4000 lx) were used to design the phycoremediation experiments having 6:18 h of dark/light period and reactor functional volume of 15L. Findings revealed that C. vulgaris was good enough to remove maximum TP (90.35%), TKN (83.55%) along 20.88% of lipid yield at 25.62 °C temperature, 60.73% PIWW concentration, and 4000 lx of light intensity, respectively. Based on the model performance and validation results, ANN showed more accuracy as compared to the RSM tool. Therefore, the findings of this study showed that C. vulgaris is capable of treating PIWW efficiently along with significant production of lipid content which can further be used in various applications including biofuel production.

ANGPTL4, a direct target of hsa-miR-133a-3p, accelerates lung adenocarcinoma lipid metabolism, proliferation and invasion.

Globally, lung adenocarcinoma (LUAD) is the most common type of lung cancer. The secreted protein angiopoietin-like 4 (ANGPTL4) has been implicated in a number of physiological and pathological processes, including angiogenesis and lipid metabolism. But the role of ANGPTL4 in LUAD remains unknown. The expression of ANGPTL4 and miR-133a-3p was confirmed by public database analysis. Xenograft model, MTT, Clone formation and EdU analysis were used to confirm the effects of miR-133a-3p/ANGPTL4 on LUAD cell proliferation and growth. Wound healing and Transwell analysis were used to elucidate the role of miR-133a-3p/ANGPTL4 in LUAD cell migration and invasion. Oil red O staining was used to confirm ANGPTL4 in LUAD lipids production. Dual-luciferase reporter gene analysis was used to demonstrate miR-133a-3p could directly bind ANGPTL4 3'-UTR. WB and PCR were used to confirm the protein expression of ANGPTL4. ANGPTL4 was significantly increased in LUAD samples, which could promote LUAD cell proliferation, migration, invasion, growth and lipid production. miR-133a-3p could directly bind to ANGPTL4 mRNA, and repress the expression ANGPTL4, resulting in suppressing LUAD proliferation and metastasis. In conclusion, miR-133a-3p/ANGPTL4 axis might be a potential biomarker and therapeutic target for LUAD patients.

Theoretical Models Constructed by Artificial Intelligence Algorithms for Enhanced Lipid Production: Decision Support Tools

Theoretical models that predict the lipid content of microalgae are an important tool for increasing lipid productivity. In this study, response surface methodology (RSM), RSM combined with artificial neural network (ANN), and RSM combined with ensemble learning algorithms (ELA) for regression were used to calculate the maximum lipid percentage (%) from Chlorella minutissima (C. minutissima). We defined one set of rules to achieve the highest lipid content and used trees.RandomTree (tRT) to simulate the process parameters under various conditions. Among the various models, results showed the optimum values of the root mean squared error (0.2156), mean absolute error (0.1167), and correlation coefficient (0.9961) in the tRT model. RSM combined with tRT estimated that the lipid percentage was 30.3% in wastewater (< 35%), lysozyme (≥ 3.5 U/mL), and chitinase (< 15 U/mL) concentrations, achieving the best model based on experimental data. The optimal values of wastewater concentration, chitinase, and lysozyme were 20% (v/v), 5 U/mL, and 10 U/mL, respectively. Also, the if-then rules obtained from tRT were also used to test the process parameters. The tRT model served as a powerful tool to obtain maximum lipid content. The final rankings of the performance of various algorithms were determined. Furthermore, the models developed can be used by the fuel industry to achieve cost-effective, large-scale production of lipid content and biodiesel.