Lipid Peroxidation Values Research Articles

Moringa oleifera hydroalcoholic leaf extracts mitigate valproate-induced oxidative status in the extraorbital lacrimal gland in a rat model

Dysfunction of the extraorbital lacrimal gland (ELG) can lead to loss of vision due to damage to the epithelium of cornea. The broad-spectrum anti-epileptic drug sodium valproate (SV) has numerous side effects. Moringa oleifera (M.oleifera) is widely used as a food and in folk medicine. The effects of orally administered SV and M. oleifera hydroalcoholic leaf extract on rat ELG were investigated in this study by analysing both antioxidant and oxidant parameters. Additionally, boron level and tissue factor (TF) activity were determined. Protein changes were detected by sodium dodecyl sulfate gel electrophoresis (SDS-PAGE). Significantly lower values of glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) and total antioxidant status (TAS) were observed in the SV group compared to the control group. Treatment with Moringa extract significantly increased SOD, CAT and TAS values in the Moringa given SV group (SVM). While no significant differences were observed between the sialic acid values of the groups, lipid peroxidation (LPO), nitric oxide (NO) and total oxidant status (TOS) values were significantly elevated in the SV group compared to the control group. Due to the effect of Moringa extract, LPO, NO and TOS levels were significantly decreased in the SVM group compared to the SV group. TF activity was not meaningfully altered between groups. Compared to control rats, oxidative stress index (OSI) level significantly increased, whereas the boron level decreased in the SV group. Moringa extract treatment noticeably reduced OSI in the SVM group. According to SDS-PAGE, decreases in the density of protein bands with molecular weights of 51, 83, and 90 kDa were observed in SV given rats compared to the other groups. These decreases were reversed by the administration of Moringa extract. Moringa extract has shown protective properties arising from antioxidant potential, especially with its very low OSI value. Individuals undergoing SV treatment and having ELG complications might consider using Moringa extract to mitigate valproate induced damage.

Assessing the impact of climate on mackerel (Scomber japonicus) quality during vehicle transportation: A transportation modeling approach

AbstractThis study investigates the impact of environmental factors on the quality changes of mackerel (Scomber japonicus), a globally significant fish species valued for its high nutritional content, especially its unsaturated fatty acids and proteins. In South Korea, marine products like mackerel undergo auction, sorting, packaging, and transportation. Assessing the fish freshness and quality during these processes, before it reaches consumers, is crucial. This research aimed to track the changes in mackerel quality indicators after the auction and correlate them with the environmental factors affecting transportation. Using a transportation model established for both summer and winter, mackerel samples were categorized by their weight (200 and 300 g). Results show poorer quality indicators in summer compared to winter, particularly in the 200 g group. Microbiological indicators such as the total viable count (0.923) and total coliforms (0.958) were most strongly correlated with the season, whereas the lipid oxidation indicators acid value (0.627) and peroxide value (0.536) were moderately correlated with the season. Interestingly, the wider standard deviation distribution observed in summer suggests that individual mackerel characteristics may influence the degree of quality changes. These findings offer insights into mackerel quality fluctuations during auction and transportation, aiding in future efforts to maintain seafood quality amidst climate change.

Rancidity development in oat during industrial processing

To improve the shelf-life of food products rich in lipids, it is important to minimize the lipid oxidation that occurs during processing, since this oxidation not only decreases shelf-life but also induces off-flavors. To evaluate the oxidation of lipids in the production of one β-glucan-rich and one protein-rich food supplement made from oats (Avena sativa), the neutral and polar lipid profile, hexanal content, peroxide value, avenanthramides and lipase activity were measured at all production steps. The concentration of antioxidants in the form of avenanthramides was also investigated. This study shows that oat lipases were fully inactivated in the wet processing part of the processing line, and the total lipid content per dry weight increased with the progression of steps toward the oat protein product. Processing had no apparent effect on avenanthramide levels. Lipid oxidation markers increased in certain processing steps. The most significant increase in hexanal and peroxide values could be seen in the spray-drying step of the process, while a much smaller increase was seen for the drum-drying. The main conclusion is that the most important factor to prolong the shelf-life of the product is to minimize the oxidation in the drying step.

Investigation of the protective effects of different forms of selenium in freezing dog semen: Comparison of nanoparticle selenium and sodium selenite.

This study aimed to investigate the protective effects of nanoparticle selenium (SeNP) and sodium selenite (SS) on preventing oxidative stress during the freezing process of dog semen. A total of six dogs were used in the study. The ejaculate was collected from dogs three times at different times by massage method. A total of 18 ejaculates were used and each ejaculate was divided in five experimental groups. The experimental groups were designed to tris extender containing no antioxidants control, 1 μg/mL SeNP1, 2 μg/mL SeNP2, and 1 μg/mL SS1 and 2 μg/mL SS2. Extended semen were equilibrated for 1 h at 4°C, then frozen in liquid nitrogen vapour and stored in liquid nitrogen (~-196°C). After thawing, semen samples were evaluated in terms of CASA motility and kinematic parameters, spermatozoa plasma membrane integrity and viability (HE Test), spermatozoa morphology (SpermBlue) and DNA fragmentation (GoldCyto). Antioxidant enzyme activity (glutathione peroxidase; GPX, superoxide dismutase; SOD, catalase; CAT) and lipid peroxidation (malondialdehyde; MDA) were evaluated in frozen-thawed dog sperm. When the results were evaluated statistically, the progressive motility, VCL, and VAP kinematic parameters in the SeNP1 group were significantly higher than the control group after thawing (p < .05). The highest ratio of plasma membrane integrity and viable spermatozoa was observed in the SeNP1 group, but there was no statistical difference found between the groups (p > .05). Although the ratio of total morphological abnormality was observed to be lower in all groups to which different selenium forms were added, compared to the control group, no statistical difference was found. Spermatozoa tail abnormality was significantly lower in the SeNP1 group than in the control and SS2 group (p < .05). The lowest ratio of fragmented DNA was observed in the SeNP1 group, but there was no statistical difference was found between the groups (p > .05). Although there was no statistical difference between the groups in the evaluation of sperm antioxidant profile, the highest GPX, SOD and CAT values and the lowest lipid peroxidation values were obtained in the SeNP1 group. As a result, it was determined that 1 μg/mL dose of SeNP added to the tris-based extender in dog semen was beneficial on spermatological parameters, especially sperm kinematic properties and sperm morphology, and therefore nanoparticle selenium, a nanotechnology product, made a significant contribution to the freezing of dog semen.

Assessment of oxidative stress parameters in summer anestrous buffaloes exhibiting differential fertility following melatonin implants treatment

For evaluating the impact of melatonin implants treatment during non-breeding season to ameliorate oxidative stress, 132 anestrous buffaloes were subcutaneously inserted with 2x4 mm absorbable slow-release melatonin implants (18 mg/50 kg b wt) at the base of left ear and 60 buffaloes were used as control. Ovarian ultrasonography and jugular vein blood sampling were carried out at 7-day interval till day 35 post-treatment or till ovulation, whichever was earlier. Control and implanted buffaloes were subjected to artificial insemination (AI) at overt or induced estrus followed by pregnancy diagnosis at day 90 post-AI. Erythrocytic lipid peroxidation (LPO) values were reduced (P&lt;0.05) in implanted buffaloes from day 21 post-treatment onwards when compared to their pre-treatment and Control group values. However, the concentrations of erythrocytic glutathione peroxidase (GPx), glutathione reductase and superoxide dismutase (SOD) were invariably higher (P&lt;0.05) following treatment as compared to their pre-treatment and Control group values. The buffaloes ovulating in Control or Treatment group revealed higher (P&lt;0.05) erythrocytic GPx in the Latter group. Also, between pregnant counterparts of Control and Treatment group, the Latter group buffaloes exhibited low (P&lt;0.05) erythrocytic LPO, and high (P&lt;0.05) erythrocytic GPx, SOD and catalase. It can be concluded that melatonin implants treatment was successful for mitigating the oxidative stress in summer anestrous buffaloes, and the status of oxidative stress parameters following treatment was better in buffaloes that ovulated or conceived subsequently.

&lt;i&gt;In vitro&lt;/i&gt; and &lt;i&gt;in vivo&lt;/i&gt; analysis of the antioxidant potentials of methanolic extract of &lt;i&gt;Tamarindus indica&lt;/i&gt; leaves

This study evaluated the antioxidant potentials of methanolic extract of Tamarindus indica leaves using in vitro and in vivo assays. reliminary qualitative screening of phytochemicals revealed the presence of alkaloids, flavonoids, saponins, tannins, cardiac glycosides and steroids. The result of the acute toxicity test of the extracts revealed the LD50 to be above 5000 mg/kg through both oral and intra-peritoneal routes. Both the in vitro and in vivo assays were carried out. The reducing power (0.45±0.007 μg/ml) was recorded for T. indica with a difference of 0.77% in comparison to the control and DPPH value (68.10 ± 0.007%) with a significant increase compared to ascorbic acid. The in vivo analysis shows that lipid peroxidation, superoxide dismutase and catalase activities have values of 22.75 ± 9.29 nmol/mg, 1.60 ± 0.03 U/ml and 0.003±0.001 μmol/mg, respectively. The values for superoxide dismutase and lipid peroxidation were significantly different compared to the control (1.48 ± 0.03 U/ml and 24.54 ± 9.29 nmol/mg, respectively) while the value for catalase activity was not significant compared to the control (0.0025 ± 0.001 μmol/mg). The liquid chromatography mass spectrometry revealed the presence of some important metabolite; linoleic acid and quassin, among others, which are antioxidant. Based on the results obtained, the T. indica leaves contain vital metabolites with antioxidant potential that could be useful in health management.

Antioxidant Activity of an Aqueous Extract of Cuttlefish Ink during Fish Muscle Heating.

The antioxidant effect of cuttlefish (Sepia officinalis) ink (CFI) was analysed in the present study. A model system consisting of minced seabream (Sparus aurata) muscle and different concentrations of an aqueous extract of CFI was subjected to a heat (50 °C) treatment for 12 days. The effects of the CFI content and the heating time on lipid oxidation (conjugated diene (CD), conjugated triene (CT), and peroxide values and fluorescent compound formation), hydrolysis (free fatty acid content) development, and changes in the fatty acid (FA) profile (polyene index (PI), unsaturated FA content, ω3/ω6 ratio) were determined. The addition of the aqueous extract of CFI led to a lower (p < 0.05) development of lipid oxidation (CD, CT, and fluorescent compound determination) and to a higher (p < 0.05) retention of unsaturated FAs (PI determination). More important effects were found with increased CFI concentrations and at advanced heating times. However, a definite effect on lipid hydrolysis development (FFA value) could not be inferred. A new approach for the beneficial use of cuttlefish ink is presented. According to the direct relationship between rancidity stability and nutritional and sensory values, the present study provides a new strategy for the quality enhancement of thermally treated seafood.

Effect of dihydroquercetin on oxidative metabolism in young athletes engaged in winter sports in a northern region

AIM: To study the effect of dihydroquercetin on the indicators of oxidative metabolism in young athletes engaged in the winter sports in the Khanty-Mansiysk Autonomous Okrug (KhMAO).&#x0D; MATERIAL AND METHODS: The indicators of oxidative metabolism were studied in a group of 56 male students (mean age 19.300.51 years) from the Khanty-Mansiysk boarding school of the Olympic reserve. These students were actively involved in winter sports such as cross-country skiing and biathlon. To assess the impact of the plant-derived antioxidant bioflavonoid Baikal dihydroquercetin (DHQ), the participants were administered a daily dose of 120 mg of DHQ for a period of 60 days. Measurements were taken before and after the exposure. Blood lipid peroxidation (LPO) products were studied, namely, lipid hydroperoxides (LHP) and products that react with 2-thiobarbituric acid products (PR-TBA). Additionally, we examined the indicators of the body's antioxidant defense system (ADS) through the assessment of total antioxidant activity (TAA) and thiol status (TS). To quantify the overall oxidative stress experienced by the participants, we calculated the oxidative stress coefficient (OSC) using the formula: OSC = LHP PR-TBA / TAA TS.&#x0D; RESULTS: The average levels of LPO values (HPL and PR-TBA) among KhMAO athletes have surpassed the upper limit of optimal values. On the other hand, the ADS parameters (TAA and TS) fall within the range of physiologically optimal values, albeit closer to the lower limit. Notably, athletes have exhibited an OSC increase that is nearly 3.5 times higher than the maximum allowable value. A quarter of the individuals examined displayed elevated HPL values, while more than 30% showed increased PR-TBA levels. A third of male athletes exhibited reduced ADS values compared to the physiologically optimal range. In total, 70.4% of skiers and biathletes in KhMAO have exceeded the OSC parameters. After two months of daily intake of 120 mg DHQ, the normalization of indicators of oxidative metabolism was observed. All parameters aligned with physiologically optimal values, except for OSC. We found a decrease in primary (LHP, 1.15 times) and secondary (PR-TBA, p=0.046) LPO indicators parallel to a significant increase in ADS indicators, specifically TAA (p=0.022) and TS (p=0.049). Conversely, there was a significant increase in ADS indicators, specifically TAA (p=0.022) and TS (p=0.049).&#x0D; Despite these positive changes, the OSC value, although significantly reduced (p 0.001) by 2.3 times compared to the initial value, remained above the upper limit of the physiological norm.&#x0D; CONCLUSION: The study demonstrated that young athletes engaged in winter sports experienced improved indicators of oxidative metabolism after a consistent two-month intake of the potent antioxidant DHQ. This led to the restoration of a balanced prooxidant-antioxidant state, enhanced overall well-being, and expedited recovery following intense physical exertion. Furthermore, our results may suggest that DHQ may contribute to prevention of non-communicable diseases in the future.

Evaluating β-cryptoxanthin antioxidant properties against ROS-induced macromolecular damages and determining its photo-stability and in-vitro SPF.

Natural antioxidants have become vital to minimize macromolecular damage caused by Reactive Oxygen Species (ROS). This study investigated the antioxidant property of β-cryptoxanthin (β-CRX) extracted fromKocuria marinaDAGII and its protective effect against macromolecular damages by generating ROS via two models: UV radiation and the Fenton reaction. β-cryptoxanthin exhibited the highest scavenging activity towards hydrogen peroxide radicals with an IC50 value of 38.30 ± 1.13μg/ml, favoring the hydrogen atom transfer mechanism. The total antioxidant capacity value of 872.0101 ± 1.84μg BHT/mg β-CRX indicated the cumulative ROS scavenging ability of β-cryptoxanthin. β-cryptoxanthin could protect against ROS-induced lipid peroxidation, protein oxidation, and DNA damage. The highest lipid peroxidation and protein oxidation inhibition values of β-cryptoxanthin against ROS were 99.371 ± 0.51% and 78.19 ± 0.15%, respectively. β-cryptoxanthin also showed a protective effect in maintaining DNA intactness against ROS-mediated DNA damage.Allium cepatest showed the non-genotoxic nature of β-cryptoxanthin and its protective effect against ROS genotoxic effects. A photo-stability study of β-cryptoxanthin toward UVA and UVB radiation showed a rapid bleaching result of UVB obeying pseudo-zero order kinetics with an average R2 value of 0.9897 and a higher k value (-6.3 × 10-11 ± 0.2M/s) than UVA (k value -3.1 × 10-11 ± 0.17M/s), signifying that UVB is more potent toward photo-degradation. The good SPF value of 23.1737 ± 0.15 showed the UV protection capability of β-cryptoxanthin. Thus, the present study suggests that β-cryptoxanthin could be a valuable antioxidant to protect against ROS-induced various macromolecular damages and act as a good UV protectant.

Flavour enhancement strategy for herbal beverages and kinetic modelling of their antioxidant and sensory properties in accelerated storage conditions

Herbal infusions and beverages are part of culinary practices and home remedies of many ethnic groups globally. Centella asiatica, Enhydra fluctuans, Eryngium foetidum and Marsilea minuta are four important herbs that used to be consumed in different East and South Asian countries due to their health benefit. The herbs are not very acceptable by the millennials due to their off flavour and bitterness. In this study, Centella asiaitica was found to be an appropriate candidate for herbal beverages among the said four herbs. A Centella based beverage was developed that can deliver high antioxidant activity together with acceptable sensory properties. Out of the two common methods of herbal beverage preparation (i.e., infusion and decoction), infused beverage was adopted owing to its better sensory acceptance. The grassy flavour of the Centella infused beverage was masked with Khasi mandarin (Citrus reticulata) peel powder (90% Centella asiatica powder with 10% Khasi mandarin peel powder). Further, the accelerated storage study revealed that Centella asiatica powder was stable during ambient storage conditions (RH 61% and 32 °C), whereas the sensory acceptability, physicochemical properties, and the antioxidant potential of the powder degraded significantly at higher temperature (45 °C and RH 82%) especially with UV exposure. The impact of storage conditions on the most critical quality parameters i.e., colour, pH, and antioxidant capacity was estimated with a first-order degradation model. All the antioxidant capacity values showed different intrinsic rates of decay and the half-life of lipid peroxidation values was shortest among the four antioxidant capacity values. This work reports a unique accelerated stability study protocol to screen the packaging and storage conditions for herbal powders. Additionally, the results suggested, Citrus peel blending is a novel approach to make herbal beverages more acceptable to consumers due to better sensory perception and enhanced stability of the herbal powder.

Changes in tra catfish flesh meat (Pangasianodon hypophthalmus) during refrigerator storage and the lipid properties of flesh meat

In this study, the lipid properties of tra catfish flesh meat (Pangasianodon hypophthalmus) during refrigerator storage as well as the properties of oil extracted from tra catfish flesh meat were investigated. The quality of the samples was maintained, with lipid content and peroxide value falling within the acceptable range after 15 days of storage. The yield of oil extracted using isopropanol:hexane at a ratio of 2:3 was higher (56.2%) than that extracted using ethanol:hexane at a different ratio. Isopropanol:hexane (2:3) also showed the highest L* (lightness/brightness) value (33.35) and the lowest b* (yellowness/blueness) value (7.35). The polyunsaturated fatty acid levels as well as the omega 3 and 6 fatty acid levels of oil were highest in this solvent at a ratio of 2:3. Thus, tra catfish flesh meat could be used as a material for lipid extraction.

Supplementation of nano-selenium (SeNPs) improved growth, immunity, antioxidant enzyme activity, and selenium retention in broiler chicken during summer season.

The present experiment was aimed at finding the optimal supplemental dose of nano-selenium in broiler chicken during the summer season for better performance in terms of growth, blood metabolites, immune response, antioxidant status, and selenium concentration in vital organs. Three-hundred-day-old Vencobb broiler chicks were randomly distributed into five dietary treatment groups with six replicates of 10 chicks each. The dietary treatments were as follows: T1 (control group), basal diet; T2, basal diet with 0.0375ppm of nano-Se; T3, basal diet with 0.075ppm of nano-Se; T4, basal diet with 0.15ppm of nano-Se; T5, basal diet with 0.3ppm of nano-Se. The experiment was carried out for 35days. The average gain and feed conversion ratio were best observed in T4 and T5. The antibody titres were significantly higher (P < 0.05) in the treated birds. At the 5th week, erythrocytic glutathione peroxidase, catalase, and superoxide dismutase activities were significantly (P < 0.05) higher and lipid peroxidation values were significantly (P < 0.05) lower in all the nano-Se-treated groups. The Se levels in the liver, breast muscle, kidney, brain, and gizzard were significantly (P < 0.05) increased with increased dietary nano-Se. Histological studies of the liver and kidney in the highest nano-Se-treated groups (T4 and T5) did not show any abnormal changes. It is concluded that supplementation of nano-selenium at 0.15ppm over and above the basal level improved the performance and protect the birds from summer stress without any adverse effect on the vital organs of chicken.

Vitamin content and MDA levels of certain white grape varieties from different altitudes in Turkey

Grapevine (Vitis vinifera L.) is regarded as one of the world's most important fruit crops in terms of acreage and commercial worth. Grapes are used to make not only wine but also fresh fruit, dried fruit, and juice. The quality of grapes or wine is significantly affected by environmental factors such as altitude, soil type, and climate. In this study, the 5 white grape varieties (Ağın beyazı, Tahannebi, Kabarcık, Şilfoni, and Besni) grown at different altitudes (800 m, 1000 m and 1200 m) in Turkey were investigated for their vitamin, and phytosterol concentrations as well as lipid peroxidation content. Şilfoni grape harvested at 800 m was found to possess the lowest lipid peroxidation content. High altitudes, were found to reduce lipid peroxidation contents in the Ağın beyazı, Tahannebi, and Kabarcık strains. The highest stigmasterol (98.34 µg/g), and beta-sitosterol (86.90 µg/g) concentrations were found within Şilfoni grape variety harvested at 800 m. The highest vitamin D-2 and D-3 levels were found in Ağın beyazı (1.39 µg/g) and Kabarcık (4.13 µg/g) grape varieties, both harvested at 1200 m. Kabarcık grape, however, revealed the highest concentrations of R-tocopherol (63.09 µg/g) and alpha-tocopherol (24.13 µg/g) at 1200 m within the samples. The highest vitamin K-2 level was determined within the Tahannebi grape (15.44 µg/g) from 1200 m. It was observed that the vitamin and phytosterol concentrations of white grape varieties tended to increase, while the lipid peroxidation values tended to decrease at high altitudes, especially at 1200 m. This study also exhibited a strong relationship among Vitamin D3, R-tocopherol, and alpha-tocopherol in the studied white grape cultivars. Keywords: Vitis vinifera; White grape; Vitamin; MDA; Altitude

Dose–responses of zinc as zinc–methionine supplements on antioxidant status, hematological parameters, immune response and the expression of IL-4 and IL-6 genes of ewes in the hot season

This study was implemented to evaluate the effects of different zinc doses as Zinc-Met supplement (Zinpro®) on the antioxidant status, blood immune cells, antibody titers, and the expression of IL-4 and IL-6 genes of ewes in the hot season. In a completely randomized design, 24 ewes were assigned to treatments as follow: 0, 15, 30 and 45 mg/kg zinc as Zinc-Met supplementation for 40 days in region with 40 °C and vaccinated against food-and-mouth disease as an immune challenge at day 30, and then blood samples were collected on day 40. Ewes were fed a basal diet containing 29.9 mg zinc/kg. The highest activity of the antioxidant enzyme and the lowest lipid peroxidation values were found in ewes receiving 30 and 45 mg/kg zinc following a linear trend. The highest lymphocytes count and antibody titers were found in ewes received 30 mg zinc/kg. There were no significant differences among treatments for the relative expression of genes. In overall, zinc supplementation non-significantly up-regulate interleukin-4 and down-regulate interleukin-6. It was concluded that zinc supplementation as Zinc-Met could enhance the antioxidant status and immune response of ewes under heat stress; supplementation of diet with 30 mg zinc/kg (300 mg/kg Zinpro®) appeared to be the most effective dose.

Microcapsules loaded with date seed extract and its inhibitory potential to modulate the toxic effects of mycotoxins in mice received mold-contaminated diet

Mycotoxins are the secondary fungal metabolites generally produced by wide range of fungi including aflatoxins (AF), ochratoxin A (OTA), fumonisins (FB), zearalenone (ZEN), and deoxynivalenol (DON). Nowadays, they are main concern to food and agricultural commodities due to undesirable health and socio-economic effect. This investigation was designed to synthesized microcapsules loaded the bioactive compounds of date seed and evaluated its inhibitory activities in mice received mold-contaminated diet. The finding revealed that the developed microcapsule is homogenous and mostly spherical with size of 2.58 μm with acceptable PDI of 0.21. The main phytochemical has been confirmed by HPLC analysis were xylose, fructose, mannose, glucose, and galactose with the respective values of 41.95%, 2.24%, 5.27%, and 0.169%. The in vivo analyses manifested that the mice received date seed microcapsules significantly (p < 0.05) improved the average daily weight gain, feed intake, liver enzymes (ALT, ALP, and AST), and lipid peroxidation values compare to mice group received mycotoxin-contaminated diet. Furthermore, encapsulation date seed bioactive compounds notably up-regulated the expression of GPx, SOD, IFN-γ, and IL-2 genes while down-regulated the iNOS gene. Consequently, the novel microcapsules loaded date seed is suggested to be considered as a promising mycotoxin inhibitor.

Enzymatic and Non-Enzymatic Antioxidant Responses of Young Tomato Plants (cv. Micro-Tom) to Single and Combined Mild Nitrogen and Water Deficit: Not the Sum of the Parts.

This study aims to perform a broad analysis of the antioxidant (AOX) responses of young tomato plants exposed to single and combined mild nitrogen (N) and water deficits through the evaluation of oxidative biomarkers, non-enzymatic and enzymatic AOX components. 'Micro-Tom' seedlings were subjected to four treatments: control (CTR; 100%N + 100%W), N deficit (N; 50%N), water deficit (W; 50%W), and combined deficits (N + W; 50%N + 50%W). An enhancement of several non-enzymatic and enzymatic components was found in plants subjected to N + W deficit, which presented higher anthocyanins accumulation (up to 103%) as well as higher levels of superoxide dismutase (SOD) transcripts at root level and of ascorbate peroxidase (APX) and catalase (CAT) transcripts at shoot level. This increase in the gene expression was also translated in augmented SOD (up to 202%), APX (up to 155%) and CAT (up to 108%) activity compared to CTR plants and the single deficits. Overall, tomato plants were able to employ defense strategies to cope with this combined deficit, as demonstrated by the higher total AOX capacity (up to 87%) compared to the single deficits, which contributed to the maintenance of their redox homeostasis, with unchanged values of lipid peroxidation and hydrogen peroxide compared with CTR plants.

Metabolic, Antioxidant and Hematologic Adjustment of Red Tilapia (Oreochromis sp) Fed Commercial Diets with Different Protein Levels and Reared in Sea Water with Biofloc

This study was conducted to evaluate the physiological response of juvenile red tilapia Oreochromis sp. (initial average body weight of 2.82 ± 0.02 g) reared in seawater with biofloc and fed extruded commercial diets containing 25, 35 and 40% of crude protein (P25, P35 and P40, respectively). After 7 weeks of assay, fish fed P35 and P40 showed similar weight gain, specific growth rate, fed conversion ratio and Fulton's condition factor. Both, P35 and P40 showed higher values of growth performance than P25 treatment. Otherwise, fish from P35 showed the lower activity of metabolic enzymes (ASAT, ALAT, GK, FBPase, G6PD and FAS) than P40. While, fish from P25 showed the highest levels of catalase activity and lower values of hepatic lipid peroxidation (like MDA). However, the decrease in protein in the diets was reflected in the decrease in Mean Corpuscular Hemoglobin (MCH) and Mean Corpuscular Hemoglobin Concentration, as well as a decrease in the concentration of albumin in the blood plasma. In conclusion, the marine biofloc allows hybrid red tilapia juveniles to be fed with 35% CP without compromising their productive performance, antioxidant system and hematological parameters.

Атерогенные нарушения у подростков с ультразвуковыми признаками жирового гепатоза и гиперурикемией

The purpose — to study the features and relationship of blood uric acid and metabolic atherogenic disorders in adolescents with fatty hepatosis. Material and methods. The study of atherogenic disorders included the following groups: 1 — adolescents with fatty hepatosis and hyperuricemia (n = 44), 2 — adolescents with fatty hepatosis and normal blood uric acid levels (n = 76), 3 (comparison group) — adolescents without acute and chronic diseases (n = 220). The study results showed higher values of average body mass index, waist circumference, triglycerides, total lipid peroxidation, glucose, HOMA index, insulin, C-reactive protein, and lower values of high-density lipoprotein cholesterol and 25-hydroxyvitamin D in the group of adolescents with fatty hepatosis and hyperuricemia, compared to the group with fatty hepatosis and normal blood uric acid levels and the comparison group. A correlation analysis in a group of adolescents with ultrasound signs of fatty hepatosis revealed a positive correlation of the uric acid level in the blood with the HOMA index (r = 0.45; p &lt; 0.001), waist circumference (r = 0.45; p &lt; 0.001), body mass index (r = 0.35; p &lt; 0.001), insulin (r = 0.34; p = 0.007), glucose (r = 0.31; p &lt; 0.024), total lipid peroxidation (r = 0.41; p &lt; 0.015), triglycerides (r = 0.36; p = 0.014), and C-reactive protein (r = 0.31; p = 0.007), and a negative correlation with the 25-hydroxyvitamin D level (r = — 0.38; p = 0.001). Conclusions. The study showed the possibility to use blood uric acid levels in fatty hepatosis in adolescents as an indicator of atherogenic metabolic disorders: insulin resistance, lipid peroxidation, dyslipidemia, chronic inflammation, and 25(OH)D insufficiency.

Exploring the Phytochemical Profiles and Antioxidant, Antidiabetic, and Antihemolytic Properties of Sauropus androgynus Dried Leaf Extracts for Ruminant Health and Production

Sauropus androgynus has become an essential plant in pharmaceutical formulations due to its beneficial antioxidant phytochemical components, participating in the antioxidant defense system and playing an important role in protecting human health. However, no research has been conducted on ruminant animals. This study aimed to evaluate the phytochemical profiles and biological potential of S. androgynus leaf extracts for ruminant health. Methanolic and hexanoic extracts from each commercially and noncommercially cultivated site were prepared over the course of five consecutive months. By means of HPLC-DAD, vitamins (ascorbic acid), essential oils (eugenol), tannins (gallic acid), cinnamic acids (caffeic acid, syringic acid, p-coumaric acid, sinapic acid and ferulic acid), and flavonoids (catechin, rutin, myricetin, quercetin, apigenin, and kaempferol) were detected. Variations in phytochemical composition were depending on solvent type but not on cultivation site or sample period. Methanolic extracts contained more phytochemicals than hexanoic extracts. Ascorbic acid and rutin were discovered to be the two most abundant phytochemicals in the methanolic extract of S. androgynus leaf, followed by essential oils, cinnamic acids, and tannins. In comparison to hexanoic extract, methanolic extract of S. androgynus demonstrated to be more efficient against oxidation scavenging: 1,1-diphenyl-2-picrylhydrazyl (IC50 = 13.14 ± 0.055 (mg/mL)), nitric oxide (IC50 = 55.02 ± 1.338 (mg/mL)) and superoxide (IC50 = 25.31 ± 0.886 (mg/mL)), as well as α-glucosidase inhibitory activity (IC50 = 9.83 ± 0.032 (mg/mL)). Similarly, methanolic was found to be more protective than hexanoic against oxidative damage in ruminant erythrocytes, with IC50 values (mg/mL) for hemoglobin oxidation, lipid peroxidation, and hemolysis of 11.96 ± 0.011, 13.54 ± 0.012, and 5.940 ± 0.005, respectively. These findings suggested that the leaves of S. androgynus are a prospective source of phytochemical substances with health-promoting qualities for ruminant production.

Gadolinium ecotoxicity is enhanced in a warmer and acidified changing ocean as shown by the surf clam Spisula solida through a multibiomarker approach

Humans have exhaustively combusted fossil fuels, and released pollutants into the environment, at continuously faster rates resulting in global average temperature increase and seawater pH decrease. Climate change is forecasted to exacerbate the effects of pollutants such as the emergent rare earth elements. Therefore, the objective of this study was to assess the combined effects of rising temperature (Δ = + 4 °C) and decreasing pH (Δ = − 0.4 pH units) on the bioaccumulation and elimination of gadolinium (Gd) in the bioindicator bivalve species Spisula solida (Surf clam). We exposed surf clams to 10 µg L−1 of GdCl3 for seven days, under warming, acidification, and their combination, followed by a depuration phase lasting for another 7 days and investigated the Gd bioaccumulation and oxidative stress-related responses after 1, 3 and 7 days of exposure and the elimination phase. Gadolinium accumulated after just one day with values reaching the highest after 7 days. Gadolinium was not eliminated after 7 days, and elimination is further hampered under climate change scenarios. Warming and acidification, and their interaction did not significantly impact Gd concentration. However, there was a significant interaction on clam's biochemical response. The augmented total antioxidant capacity and lipid peroxidation values show that the significant impacts of Gd on the oxidative stress response are enhanced under warming while the increased superoxide dismutase and catalase values demonstrate the combined impact of Gd, warming & acidification. Ultimately, lipid damage was greater in clams exposed to warming & Gd, which emphasizes the enhanced toxic effects of Gd in a changing ocean.