You have accessJournal of UrologyStone Disease: Basic Research & Pathophysiology II1 Apr 2018MP24-12 TRANSDIFFERENTIATION FROM WHITE ADIPOCYTES TO BEIGE CELLS PREVENTS KIDNEY STONE FORMATION Teruaki Sugino, Kazumi Taguchi, Rei Unno, Shuzo Hamamoto, Ryosuke Ando, Atsushi Okada, Tohru Mogami, Keiichi Tozawa, Kenjiro Kohri, and Takahiro Yasui Teruaki SuginoTeruaki Sugino More articles by this author , Kazumi TaguchiKazumi Taguchi More articles by this author , Rei UnnoRei Unno More articles by this author , Shuzo HamamotoShuzo Hamamoto More articles by this author , Ryosuke AndoRyosuke Ando More articles by this author , Atsushi OkadaAtsushi Okada More articles by this author , Tohru MogamiTohru Mogami More articles by this author , Keiichi TozawaKeiichi Tozawa More articles by this author , Kenjiro KohriKenjiro Kohri More articles by this author , and Takahiro YasuiTakahiro Yasui More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2018.02.765AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Our recent studies have revealed that adipocytokines secreted by adipocytes are important for the formation of kidney stones. β3-adrenergic receptor agonist is reported to differentiate white adipocytes into beige cells, which improves metabolic syndrome via the specific protein uncoupling protein 1 (UCP1) expression. In this study, we investigated the effect of transdifferentiation from white adipocytes to beige cells on kidney stone formation. METHODS Mice were administered daily intra-abdominal injection of saline (control group) or 1.0 mg/kg β3-agonist CL316243 (β3 group) for 12 days. From days 6 to 12, we induced renal crystal deposits by daily intra-abdominal injection of 80 mg/kg glyoxylate. Fat tissues and kidneys were extracted at days 0, 6, and 12. We examined the morphology as well as histology of fat tissues. Total RNA of fat tissue and kidneys were isolated and reverse-transcribed into double-stranded cDNA. Then, the expression of UCP1, adipocytokines, and stone-related genes was assessed using quantitative real-time polymerase chain reaction (PCR). The formation of renal crystal deposits was observed using polarized light microscopy, and percentages of the depots as the total tissue area of the renal cross-section were expressed using the Image Pro software. RESULTS β3-agonist treatment reduced lipid droplets in adipocytes and provided differentiation from white adipocytes into beige cells. The expression levels of UCP1 and adiponectin in the adipose tissue in the β3 group increased by 8.1-fold and 4.2-fold compared with those in the control group at day 12 (P < 0.01, P = 0.01). The expression levels of IL-6 and osteopontin decreased by 0.3-fold and 0.1-fold (p = 0.03, p = 0.02). In the kidneys, the expression levels of SOD1 increased by 2.3-fold, and EMR1 decreased by 0.4-fold (P = 0.04). The formation of renal crystal deposits decreased to 17.0% in the β3 group (P = 0.03). CONCLUSIONS Our results showed that transdifferentiation from white adipocytes into beige cells suppressed renal crystal formation by reducing pro-inflammatory adipocytokine secretion and improving antioxidant action. This is the first report on the therapeutic role of beige cells for kidney stone formation. © 2018FiguresReferencesRelatedDetails Volume 199Issue 4SApril 2018Page: e294-e295 Advertisement Copyright & Permissions© 2018MetricsAuthor Information Teruaki Sugino More articles by this author Kazumi Taguchi More articles by this author Rei Unno More articles by this author Shuzo Hamamoto More articles by this author Ryosuke Ando More articles by this author Atsushi Okada More articles by this author Tohru Mogami More articles by this author Keiichi Tozawa More articles by this author Kenjiro Kohri More articles by this author Takahiro Yasui More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...