Let T>2 be an integer and T={1,2,…,T}. We show the existence of the principal eigenvalues of linear periodic eigenvalue problem -Δ2u(j-1)+q(j)u(j)=λg(j)u(j), j∈T, u(0)=u(T), u(1)=u(T+1), and we determine the sign of the corresponding eigenfunctions, where λ is a parameter, q(j)≥0 and q(j)≢0 in T, and the weight function g changes its sign in T. As an application of our spectrum results, we use the global bifurcation theory to study the existence of positive solutions for the corresponding nonlinear problem.