Linear Hypoplasia Research Articles

More severe stress markers in the teeth of flanged versus unflanged orangutans (Pongo spp.).

We compared an early life stress indicator, linear enamel hypoplasia (LEH), in the canine teeth of two male orangutan (Pongo spp.) morphs. Flanged males have large bi-discoid cheek pads and a laryngeal throat pouch, and they exhibit either the same or higher levels of the stress hormone cortisol throughout development compared with unflanged males, which lack secondary sexual characteristics. Such "developmental arrest" is hypothesized to either reflect a response to experienced high stress (Hypothesis 1), or an adaptation to avoid elevated stress levels and/or having experienced lower stress levels (Hypothesis 2) during early life. As LEH defect depth has been shown to reflect the severity (i.e., intensity and/or duration) of early life stress events, we examined whether unflanged males have shallower LEH defects than flanged males. Flanging status was assessed by measuring the faces of preserved skins. Canine height (N=37) was measured in the same individuals to assess commonality between morphs. LEH defect depths were analyzed using a standardized confocal profilometry method (N=34). Flanged males have significantly deeper LEH defects than unflanged adult males. Canine projected crown heights are similar across males regardless of morph. Evidence from great apes shows that, when comparing canines with similar growth patterns, deeper defects reflect more severe stress events during development. Thus, our results suggest that "developmental arrest" of unflanged males is not a response to having experienced stress, but rather an adaptation to avoid the physiological impacts associated with chronic stress and/or experiencing lower stress levels.

Stress, health and demography at Charity Hospital Cemetery #2 (AD1847–1929)

AbstractThe physiological toll of poverty—from inadequate nutrition, higher disease loads, dangerous and taxing occupations, to limited health care—constitutes a form of structural violence. This violence is often embodied on skeletal tissues as signs of systemic biological stress. Here, we explore the skeletal manifestations of systemic biological stress on the bodies of those interred at Charity Hospital, an indigent hospital, in New Orleans, Louisiana. Given Charity's mission to serve the poorest residents of New Orleans, we expected to see widespread evidence of systemic biological stress in the skeletal remains of deceased patients from the hospital. Two skeletal collections from Charity Hospital Cemetery #2 were assessed for paleodemography and paleopathology, specifically this latter analysis focuses on the presence and severity of periosteal reactive new bone growth, linear enamel hypoplasia and porotic hyperostosis/cribra orbitalia, all skeletal signs of systemic stress throughout one's lifetime. We find that the remains show some signs of systemic stress. While this contradicts the trend shown in the hospital's records, it should be noted that indicators of systemic stress cannot map perfectly onto the health of a past population. It is possible that the individuals who comprised these two samples died of diseases and maladies that did not leave any skeletal indicators; an expectation that is reinforced by the fact that this cemetery was conceived as a response to multiple yellow fever epidemics in the 1840's, a disease that does not leave any skeletal signs.

Conventional microscopy makes perikymata count and spacing data feasible for large samples.

Current methods of quantifying defects of dental enamel (DDE) include either gross or low-level examination for linear enamel hypoplasia, histological analysis of striae of Retzius, or scanning electron microscopy (SEM) of a tooth or a tooth cast. Gross examination has been shown to miss many defects. Other methods can be destructive, require transporting samples, and are expensive. Here, we show that digital light microscopy (DLM) can be used for the analysis of DDE as indicated by widened perikymata spacing (WPS). This method takes advantage of high-power (100×) microscopy, but is non-destructive, can be implemented almost anywhere, and is inexpensive. As proof of concept, we created photomontages of labial surfaces of five human canines from images made using DLM and SEM. We counted and measured the widths of all visible perikymata for each imaging modality and fit measurements to a negative curve representing the expected values for each tooth. We calculated residuals for each measurement. WPS were defined when R2 was in the 90th percentile, and were considered matched in DLM and SEM images when observed within the same decile of the tooth surface. There were more WPS detected in the images derived from DLM than from SEM. Overall, the data derived from the two imaging modalities provided similar information about the frequency and timing of stress during dental development. The method described here allows for DDE data acquisition as WPS from large samples, making feasible population-level studies that reflect sophisticated understanding of dental development.

Oral conditions of the pre-Hispanic mummies of Gran Canaria (Canary Islands, Spain)

ObjectiveTo analyse the oral conditions of pre-Hispanic mummies from Gran Canaria (5th-11th centuries AD), comparing the results with published data from the non-mummified population. Materials440 teeth and 764 alveoli of 30 adult mummies. MethodsMacroscopic examination of pathological and non-pathological features of the oral cavity, using standardized criteria. ResultsThe mummies reveal frequent dental caries (11.8%), especially affecting molars (27.6%), a high prevalence of calculus (66.3%) and periodontal disease (34.9%). The average wear is characterized by extensive dentine exposure. Periapical lesions (10.6%) and antemortem tooth loss (AMTL) (15.9%) are common. A high percentage of individuals exhibit linear enamel hypoplasia (LEH) (84%). Except for periodontitis, the data indicate the absence of significant statistical differences between the mummies and the skeletonized sample. ConclusionsThe profile of the dental pathologies of the mummies indicates a carbohydrate-rich diet that contained abrasive grit from the stone querns used to grind cereals. Comparison of the oral conditions of mummified and skeletonized remains shows no differences in access to food resources, reinforcing the recent rejection of the traditional interpretation of the mummies as the pre-eminent status group of Canarian society. SignificanceThis is the first study to delve into the oral conditions of pre-Hispanic mummified remains from Gran Canaria. The results have implications for the framing of research questions based on the social status of these mummies. LimitationThe preserved sample of mummified remains of ancient Canarians is small. Suggestion for further researchComparative analysis from a diachronic perspective would improve understanding of the historical development of ancient Canarians.

Exploring dietary practices in non‐adults of the Late Bronze Age Southern Urals: A perspective from dental attributes

AbstractThe article provides a bioarchaeological perspective on the dietary practices of the Late Bronze Age Southern Ural communities, which once inhabited the steppe and forest‐steppe zones of modern Bashkiria (Russia), via studying the pathological dental conditions and aspects of dental tooth use in skeletal remains from four kurgan cemeteries. As children and adolescents constitute the majority of the skeletal remains, the focus is on non‐adult dentition. The cemeteries were used by Srubnaya and Srubnaya‐Alakul communities that are believed to have been settled pastoralists. Despite the absence of convincing archaeological evidence, the existence of secondary‐role agriculture in these communities has not been ruled out. The studied sample includes 38 non‐adults (<18 years of age). The dental attributes studied were linear enamel hypoplasia (LEH), caries, dental calculus, dental wear, and antemortem enamel chipping. In total, the sample showed no cases of caries, a very high frequency of dental calculus, and a frequency of enamel chips that increased with age. The rate of dental wear seems to be mild, though there is a need for comparative data on non‐adults. The frequency of LEH was relatively low and the defects peaked at about 2–4 years of age. Basically, the Bashkirian Late Bronze Age (LBA) non‐adult sample fits well into the larger picture of the Bronze Age pastoralists from the Volga‐Ural and Trans‐Ural steppes. Given the archaeological evidence and the prevalence of the studied dental characteristics, the data are consistent with a society primarily reliant on meat and milk products.

Estimation of age at onset of linear enamel hypoplasia. New calculation tool, description and comparison of current methods.

Enamel Hypoplasia (EH) is known to be a useful indicator for wide range of detrimental factors in early childhood in past populations, such as nutritional disturbances, mechanical trauma, disease, metabolic, and/or genetic disorders. EH may be divided into three categories: pits, grooves, and lines, where the last two are referred to as "Linear Enamel Hypoplasia" (LEH). The regularity of enamel formation allows retrospective determination of the age of LEH formation. The current article reviews and compares the best-known methods used to estimate age at LEH formation and provides a new computational tool. Growth curves for canines and incisors were developed based on tooth growth tables by previous authors. Optimal models were selected using the Akaike Information Criterion. A Microsoft Excel spreadsheet was created to calculate age at LEH formation using the most common methods. All method results were compared with an archaeological sample (44 teeth of 18 individuals from an early modern cemetery from Wrocław, Poland) and a theoretical model. The results of the methods were compared pairwise with Bland-Altman plots. The current article provides a quick and easy-to-use tool for analyzing LEH chronology and comparing the results of different methods. As shown by the Bland-Altman plots, most methods provide approximately consistent results for LEHs formed at around 2-3years of age. However, LEHs formed particularly early or late are more prone to discrepancies between different methods. Comparison of the age at LEH formation obtained by different methods should be done carefully - and the new LEH calculation tool with optimized equations provided in this publication can facilitate this process.

A reflection of the unfavorable living conditions caused by the First World War in the diet and health of a 10‐year‐old boy (1907–1917) from Vetlá cemetery, Bohemia

AbstractAlthough First World War battles did not directly affect the territory of all participating states, the conflict brought suffering in the form of a decline in their inhabitants' economic situation. This was also true in the Czech Lands. Opportunities for studying the impact of the period's difficult living conditions on the human body are very limited. This study presents pilot research into the possible influence of the wartime environment on children's lives through the example of a 10‐year‐old boy (1907–1917) from a family of landowners. The circumstances of his death and burial, demonstrated in coffin adjustment, confirm that the war influenced conditions at the time. We therefore considered the possible osteological and nutritional manifestations of the stress of that time, such as difference between biological and chronological age, the presence of skeletal developmental anomalies, signs of nonspecific stress in the form of linear enamel hypoplasia and Harris lines, and diet, also in the family context. The results showed no significant difference between the boy's biological and chronological ages nor body height disorder. There were seven developmental anomalies on his skeleton, some also found in other family members, which supports documented family relationships. Co‐occurrence of signs of nonspecific stress and isotopic shifts were also found, indicating the possible presence of nutritional stress before his wartime death.

Heterogeneous frailty and the expression of linear enamel hypoplasia in a genealogical population.

Linear enamel hypoplasia (LEH) is a common skeletal marker of physiological stress (e.g., malnutrition or illness) that is studied within and across populations, without reference to familial risk. We examine LEH prevalence in a population with known genealogical relationships to determine the potential influence of genetic heritability and shared environment. LEH data of 239 individuals from a single population were recorded from the Ohio State University Menegaz-Bock collection dental casts. All individuals were of known age, sex, and genealogy. Narrow-sense heritability estimates were obtained for LEH presence and count data from all unworn, fully erupted teeth (excluding third molars) using SOLAR (v.8.1.1). Age, sex, and age-sex interaction were included as covariates. Models were re-run with a household effect variable. LEH persists across generations in this study population with moderate, significant heritability estimates for presence in four teeth, and count in four teeth (three teeth were significant for both). When a household effect variable was added, no residual heritability remained for LEH count on any tooth. There was no significant household effect for three of the four teeth that had significant heritability estimates for LEH presence. Age was a significant covariate. Further analyses with birth year data revealed a secular trend toward less LEH. This study provides evidence for familial risk of LEH (genetic and environmental) that has consequences for the broad use of this skeletal marker of stress. These results have repercussions for archaeological assemblages, or population health studies, where genetic relatives and household groups might be heavily represented.

Cold Discomfort: A Model to Explain Repetitive Linear Enamel Hypoplasia Among Pan troglodytes and Pan paniscus

A geographically and temporally widespread pattern of repetitive episodes of developmental stress, recorded as furrows of linear enamel hypoplasia (LEH) in most recent and fossil apes, requires explanation. I compared observations of LEH recurrence among museum specimens of Pan troglodytes and Pan paniscus with historical weather records of seasonally recurrent combinations of lower temperature, higher rain and wind (“cold discomfort”). I imaged samples of 34 canine teeth (N = 20 animals, 54 independent LEH) from P. troglodytes from Fongoli, Senegal, and Taï Forest, Côte d’Ivoire and P. paniscus from the Democratic Republic of Congo with a scanning electron microscope and counted perikymata between and within LEH events. I converted counts to time using published Retzius periodicities (the number of days taken to form enamel layers, visible in thin sections) and compared their recurrence and duration to seasonal peaks of incidence and the intensity of cold discomfort. Using the longest Retzius periodicity (9 days), chimpanzees and bonobos show LEH lasting about 7–9 weeks, respectively, recurring annually. Most bonobos also show just-under semiannual recurrence of LEH. “Colder, wetter, windier” weeks recur annually at the P. troglodytes locations and semiannually at P. paniscus sites. When the combination of “below median temperatures, above median rain and winds” peaks in intensity and incidence over a 7-week period, daily “minimum hourly” temperatures average 20–21°C (7–9°C below lower critical body temperature for chimpanzees) with wind 3 times and rainfall 30 times higher than usual. These findings suggest that seasonal cold discomfort may be an important factor in episodic enamel hypoplasia in many nonhuman primates.

UN RECORRIDO POR LOS HUESOS OLVIDADOS DEL DOLMEN DE PEDRAS GRANDES (ODIVELAS, PORTUGAL)

The Dolmen of Pedras Grandes (Odivelas, Lisboa, Portugal) was discovered and excavated at the end of the 19th century by Carlos Ribeiro. In 2004, this monument was re-excavated by Rui Boaventura and a complete study was conducted. The Dolmen of Pedras Grandes presents a polygonal chamber and a very short passage and may have had a short period of burial activity in the 4thmillennium as indicated by the radiocarbon dates and the “archaic” artefacts. The relative and absolute dating enable us to assign this monument to the initial phase of megalithic funerary monumentality in south-central Portugal. Moreover, the recovery of human skeletal material offers an opportunity to obtain data on the biological profile and the health status of the human community who buried their dead in this megalithic tomb. In this paper are present new radiocarbon dates and isotopic analyses obtained from human bone samples from this tomb and also a complete anthropological study of the human skeletal material recovered in this monument. At least 13 individuals are represented in the skeletal assemblage, including 8 adults (> 16 years) and 5 non-adults, although this is certainly an underestimate due to the poor preservation of the bone assemblage. Young children, under the age of three years, are missing. A small number of pathologies were observed, including cranial trauma, degenerative joint disease and oral pathologies. The high frequency of stress indicators, more specifically linear enamel hypoplasia, is a notable feature of this assemblage. The new data are discussed in the context of the problem of the origin of megalithic monumentality in Central and Southern Portugal

Were late Gomphotheres (Plio-Pleistocene) of the Siwaliks at more Stress as Compared to early Gomphotheres (middle to late Miocene)?

Gomphotheres existed in the Siwaliks from the middle Miocene (14.2Ma) to the middle Pleistocene (0.8Ma) and became extinct later on. In this paper, we tried to discuss the reasons of such extinction of gomphotheres in the lower Pleistocene time span by considering Linear Enamel Hypoplasia (LEH) among 114 isolated tooth samples to assess whether ecological changes correlate with the stress factor in gomphotheres. For this purpose, the Siwalik gomphotheres were divided into two Groups viz. early gomphotheres (middle Miocene to late Miocene) and late gomphotheres (Pliocene to middle Pleistocene). We presented the hypothesis, that as the gomphotheres are characterized by the brachydonty and relied on browsing for their feeding while inhabiting the semi forest land setting thus, expected to have higher stress in Plio-Pleistocene time span as vegetational change around ~6 Ma may have exerted stress on late gomphotheres. The results for the occurrence of frequency of LEH indicated severe ecological stress in late gomphotheres (33%). The significant differences were found (P < 0.05) among the early gomphotheres and late gomphotheres which can be correlated to the vegetational change from C3 to C4, higher aridity indices and intensified seasonality after the late Miocene vegetational shift which may have resulted in substantial faunal turnover, extinction and speciation. We assume that such palaeoecological changes forced a competition with more pronounced grazers like of family Elephantidae and Bovidae resulting in extinction of gomphotheres during the late Pleistocene in the Siwaliks of Pakistan.

New and Emerging Prospects for the Paleopathological Study of Starvation

Starvation represents a significant contributor to morbidity and mortality, past and present, and is therefore of critical importance to the field of paleopathology. Scholars have previously argued that while critical to understanding past human health, starvation is often not directly observable in skeletal remains. But is this assessment still valid today? In re-evaluating this assessment, this paper discusses new developments in the analysis of (1) the “hunger osteopathies” (osteoporosis with some overlay of osteomalacia), (2) skeletal signs of arrested growth such as Harris lines and Linear Enamel Hypoplasia (LEH), and (3) carbon and nitrogen stable isotope analysis of skeletal remains. Periods of starvation are known to cause these visible and chemical alterations within skeletal remains, but these phenomena are complex, multi-etiological, and approaches to evaluate them are often fraught with a lack of standardization and specificity. An interdisciplinary approach synthesizing multiple lines of osteological and dental evidence, borrowing anatomical and medical research, and implementing new advancements in computer modeling, imaging modalities, and chemical micro-sampling may theoretically aid in inferring starvation bioarchaeologically.

Evaluating life history trade‐offs through the presence of linear enamel hypoplasia at Pueblo Bonito and Hawikku: A biocultural study of early life stress and survival in the Ancestral Pueblo Southwest

Due to the indelible nature of enamel, bioarchaeologists use linear enamel hypoplasia (LEH) to detect early investments in surviving stress and have identified an association between LEH presence and constraints in growth and maintenance as well as an increased susceptibility to future stress events. This study evaluates heterogenous frailty and susceptibility to death in relation to episodes of early life stress, as reflected by LEH presence, in the Ancestral Pueblo Southwest. This study hypothesizes that LEH presence will be associated with decreased survivorship and an increased likelihood of mortality in both samples. This study uses two samples, one from Pueblo Bonito (A.D. 800-1200; n = 28) and the second from Hawikku (A.D. 1300-1680; n = 103). Kaplan-Meier survival analysis with a log-rank test was used to evaluate the effect of LEH presence on survivorship for the two samples. Survival analysis reveals statistically significant differences in mortality risk between individuals with and without LEH for the Hawikku sample, but no significant differences for the Pueblo Bonito sample. The results demonstrate differences in the response to early life stress at the Hawikku and Pueblo Bonito sites, likely reflecting context. The Pueblo Bonito sample represents a high-status group, and survival following LEH may be the result of cultural buffering. Hawikku dates to a period associated with increased levels of disease and malnutrition as well as Spanish colonization. This environment may have exacerbated mortality risk for individuals in the region who survived early life stress and signifies the consequences of European colonialism in the New World.

The impact of using new and conventional methods for the age-at-death estimation in a Czech medieval population (Mikulčice, 9th-10th century): the relationship between age-at-death and linear enamel hypoplasia.

Recent advances in age-at-death estimation from the skeleton indicate that some of the most commonly used methods based on linear regression provide different results compared to new techniques using Bayesian statistics, and underestimate individuals over 60 years old which leads to biased prehistoric lifespans. The question is how the choice of age-at-death estimation method can influence subsequent comparisons between different populations or further analysis, such as assessment of the effect of early stress on mortality in adult individuals. The aim of our work is twofold: firstly, to test the differences between age estimation methods evaluating one indicator (the auricular surface), namely the original (Lovejoy et al. 1985), revised (Buckberry & Chamberlain 2002) and newly developed (Schmitt 2005) methods, on the Early Medieval adult population from Mikulčice - IIIrd church (Czech Republic, Central Europe). The secondary objective is to assess whether the different age distributions based on the different methods have an impact on age-dependent analyses, in this case the relationship between LEH and age-at-death. Our results showed that in the adult population from Mikulčice - IIIrd church, the original and revised methods provided different mortality profiles: the proportion of individuals older than 60 years acquired using Lovejoy's method was only 6.7%, while the newer methods increased the proportion to 26.7% (Buckberry & Chamberlain 2002) and 23.9% (Schmitt 2005). The choice of age-at-death estimation, and thus the different age distributions, also resulted in differences in the achieved age of individuals with and without stress markers, and specifically in the significance of the differences found. This finding seeks to draw attention to the fact that inconsistency in the use of different age-estimation methods can influence the results of further analyses and cause problems when comparing burial grounds.

Linear enamel hypoplasia in large-bodied mammals of Pleistocene northern Vietnam, with a special focus on Pongo

The Coc Muoi fauna provides a good example of the type of tropical mammalian communities that existed in northern Vietnam during the late Middle Pleistocene. The first results of the analysis of hypoplasia indicated that rhinoceroses and wild cattle were exposed to multiple physiological and psychological stress events specific to age [Bacon et al., 2018. A rhinocerotid-dominated megafauna at the MIS 6-5 transition: The late Middle Pleistocene Coc Muoi assemblage, Lang Son province, Vietnam. Quat. Sci. Rev. 186, 123–141].In this paper, we aim to supplement the study of hypoplasia in the orangutans (Pongo) from Coc Muoi and, more widely, from different Pleistocene faunas. To address this issue, we conducted a macroscopic analysis of linear enamel hypoplasia [LEH] on Pongo from three collections: Coc Muoi (148–117 ka), Duoi U'Oi (70–60 ka), and Tham Khuyen (>475 ka).Such comparative analysis based on isolated teeth is constrained by numerous biases including: the small datasets; the differential representation of tooth types; the difficulty in distinguishing first from second molars; the small number of individuals [MNI]; the differential representation of males versus females. The data analysis has been divided into two parts: (1) an analysis of the frequency and expression of LEH on incisors, premolars, and molars (three sites), and (2) an analysis of the frequency and expression of LEH on a set of canines (Tham Khuyen). We used a reference sample composed of 17 adult and 10 immature Pongo individuals to determine the age range of fossil Pongo individuals, at the time of the defects.Results show that hypoplasia was a common phenomenon in Pleistocene Pongo: two individuals at Coc Muoi; 2 out of 3 individuals at Duoi U’Oi; and 4 out of 6 individuals at Tham Khuyen. They experienced multiple stresses between ~2 and 5 years of age, a period of great vulnerability for immature individuals. The occurrence of accentuated lines of hypoplasia on canine crowns of Tham Khuyen suggests a greater intensity of the stressor, in a time range consistent with the long dietary and behavioral transition of the weaning. In terms of paleoecology, Pleistocene orangutans from the Asian mainland could survive in different environmental conditions than those they occupy today. Various sources (archaeozoology, geological context, and ecology of wild populations), suggest that they might have been larger apes than extant orangutans, living in limestone forests on hills and tower karsts.

Bioarchaeological reconstruction of physiological stress during social transition in Albania

ObjectiveWe test the hypothesis that physiological stress increased in response to increasing social turmoil following waves of colonization and social transition. The ways local conditions, including variation in geography, environment, and levels of urbanization impact physiological stress are also explored. MaterialsIn Albania, the historic period is a sequence of different waves of colonization. Skeletal data come from three Albanian archaeological sites: Apollonia (n = 231), Durrës (n = 246), and Lofkënd (n = 129). MethodsPrevalence of cribra orbitalia, porotic hyperostosis, linear enamel hypoplasia, and periosteal new bone formation are analyzed using chi-square and logistic regression tests. ResultsWe observe increased skeletal manifestations of physiological stress between prehistoric and historic groups, but physiological stress is generally consistent through time. ConclusionsGeneral increase in skeletal pathology between prehistoric and historic periods corresponds to broad increases in political unrest associated with colonization spanning the entire historic period. However, little difference in physiological stress across colonization episodes (Greek, Roman, Byzantine, Bulgarian, Ottoman) suggests skeletal health is affected similarly by colonization, regardless of particularities in method and type of colonial control. SignificanceExamining human response to social change across broad time scales is useful in identifying broad patterns in the human experience. LimitationsExploring variation across broad time scales and multiple sites is potentially problematic because confounding factors could impact results and interpretations. Suggestions for further researchEnvironmental, social, and geographic differences, likely impacted the lives and lifestyles of individuals living in the past and should be explored further to understand the nuances in local response to colonization.

An integrative approach to studying plasticity in growth disruption and outcomes: A bioarchaeological case study of Napoleonic soldiers.

The aim of this study was to investigate how much variation in adult stature and body mass can be explained by growth disruption among soldiers who served in Napoleon's Grand Army during the Russian Campaign of 1812. Linear enamel hypoplasia (LEH) were recorded as representations of early life growth disruption, while the impact on future growth was assessed using maximum femur length (n = 73) as a proxy for stature and maximum femoral head diameter (n = 25) as a proxy for body mass. LEH frequency, severity, age at first formation, and age at last formation served as explanatory variables in a multiple regression analysis to test the effect of these variables on maximum femur length and maximum femoral head diameter. The multiple regression model produced statistically significant results for maximum femur length (F-statistic = 3.05, df = 5 and 67, P = .02), with some variation in stature (adjusted r2 = 0.13) attributable to variation in growth disruption. The multiple regression model for maximum femoral head diameter was not statistically significant (F-statistic = 1.87, df = 5 and 19, P = .15). We hypothesized stress events during early life growth and development would have significant, negative, and cumulative effects on growth outcomes in adulthood. The results did not support our hypothesis. Instead, some variables and interactions had negative effects on stature, whereas others had positive effects. This is likely due to catch-up growth, the relationship between acute and chronic stress and growth, resilience, and plasticity in human growth over the life course.

Dental corrosion in preindustrial societies: A case study of a child from “Pedra do Cachorro” dating to 1,470 BP, Northeastern Brazil

Systemic vomiting resulting from mental disorders and the high intake of acidic beverage in industrial societies result in a relatively elevated frequency of dental corrosion. In the past, however, this type of chemical dental wear was rather rare. Here we describe the case of a 3-year-old child dated to 1470±30BP from the archaeological site of Pedra do Cachorro (northeastern Brazil) that presents a unique pattern of chemical wearing compatible with dental corrosion. We integrate this observation with a broader characterization of buccal health including caries, periapical lesions, dental calculus and periodontal bone resorption. Osteological markers of physiological imperilments such as linear enamel hypoplasia (LEH) and transverse radiopaque lines (Harris lines) are also considered.

Assessing weaning stress – Relations between enamel hypoplasia, δ18O and δ13C values in human teeth obtained from early modern cemeteries in Wroclaw, Poland

ObjectivesThe aim of this paper is to assess linear enamel hypoplasia (LEH) on canines of adults from early modern Wroclaw cemeteries to compare δ13C and δ18O values in enamel formed before, during and after LEH formation, and to attempt to find a link between the selected indicators of the weaning process. Material and methods15 permanent canines with visible LEH were selected for analysis. Age at LEH formation was estimated using the Reid and Dean method. The perikymata count was used to estimate the duration of LEH formation. Incremental stable oxygen and carbon isotopes analysis was performed on tooth enamel to infer stress related to weaning and dietary patterns. Kruskal–Wallis ANOVA, Mann–Whitney U test and Spearman correlation were used in statistical inference. ResultsThe average age of LEH formation was 3.14 years, with an average duration of 111 days. Stable carbon isotope values consistently increase in 73% of the individuals sampled. The values of δ18O do not show a dominant pattern. The average value of δ13C increases over the time of enamel formation. The δ13C values collected below LEH were negatively correlated with δ18O values and the duration of LEH termination. ConclusionsMarking of carbon and oxygen isotopes indicates different stages of weaning. Advancements in diet change (determined by higher δ13C) corresponds with faster recovery after physiological stress episode. There is no evidence for different breastfeeding models between distinct parishes in early modern Wroclaw.

Social Status and Its Relationship to Non-specific Stress at Late Iron Age Non Ban Jak, Northeast Thailand

The rise of social inequality is a key development in human history and is linked to deteriorating health. These associated health impacts are poorly understood for Iron Age (420 B.C.–A.D. 500) northeast Thailand. To clarify this issue we investigate whether social status differences influence non-specific stress at the site of Non Ban Jak (A.D. 300–800), which comprises two separate burial mounds. These mounds are thought to represent the neighborhoods of two distinct social groups at the site. Quantitative analyses were used to explore differences in grave goods among the adults of Non Ban Jak (N = 47). Long bone lengths, ages at death, and linear enamel hypoplasia (LEH) were examined to explore differences in non-specific stress on the basis of age, sex, burial mound, and mortuary phase. Results demonstrated that older adults of both sexes, males of all ages, and west mound individuals received greater grave good quantities and may therefore have been of higher social status. West mound individuals were taller and had a lower prevalence of LEH compared to those from the east mound. Although female LEH prevalence and mortality were reduced relative to males, decreasing stature over time and high neonatal mortality indicated greater female stress. Lower-status individuals may therefore have suffered increased stress relative to those of higher status. Artifactual and epigraphic evidence supports the suggestion of sex- and class-based status differences at Non Ban Jak, legitimized and perpetuated through the adoption of residential burial and new religious ideologies. การปรากฎขึ้นของความไม่เท่าเทียมทางสังคมเป็นพัฒนาการสำคัญอย่างหนึ่งในประวัติศาสตร์มนุษยชาติและเชื่อมโยงกับสุขภาพที่เสื่อมลง ความรู้ความเข้าใจเกี่ยวกับสุขภาวะที่เกี่ยวเนื่องกับความไม่เท่าเทียมทางสังคมในสมัยเหล็ก (ประมาณ ๒,๔๐๐ – ๑,๕๐๐ ปีมาแล้ว) ในภาคตะวันออกเฉียงเหนือนี้ยังมีน้อย ดังนั้น เพื่อสร้างความเข้าใจให้ชัดเจนในเรื่องนี้ เราจึงได้ศึกษาตรวจสอบว่าความแตกต่างด้านสถานะทางสังคมมีอิทธิพลต่อความตึงเครียดหรือไม่ ทั้งนี้ เราใช้หลักฐานทางโบราณคดีจากแหล่งโบราณคดีโนนบ้านจาก (กำหนดอายุระหว่าง ๒,๓๐๐ – ๑,๒๐๐ ปีมาแล้ว) ซึ่งมีเนินหลุมฝังศพ ๒ เนิน เราเชื่อว่าเนินดินหลุมฝังศพทั้งสองเนินนี้เป็นแหล่งฝังศพของกลุ่มคน ๒ กลุ่มที่เป็นเพื่อนบ้านกันหรือมีความใกล้ชิดทางสังคม เราใช้วิธีการวิเคราะห์เชิงปริมาณในการศึกษาตรวจสอบความแตกต่างของวัตถุอุทิศที่พบร่วมกับหลุมฝังศพของคนในวัยที่เป็นผู้ใหญ่ นอกจากนี้ เรายังวิเคราะห์ความยาวของกระดูกแขน-ขา อายุเมื่อตาย และการเสื่อมผุของเคลือบฟัน เพื่อตรวจสอบระดับความแตกต่างของความตึงเครียดตามเพศ อายุ/วัย กลุ่มสังคม และช่วงเวลา ผลการศึกษาพบว่าผู้ใหญ่วัยสูงอายุทั้งเพศหญิงและชาย ผู้ชายทุกวัย และกลุ่มคนจากเนินฝังศพด้านตะวันตกมีจำนวนวัตถุอุทิศมากกว่ากลุ่มคนจากเนินฝังศพทางตะวันออก และดังนั้นคนที่ถูกฝังในเนินดินฝังสพทางตะวันตกอาจจะมีสถานภาพสูงกว่าคนที่ถูกฝังในเนินดินหลุมฝังศพทางตะวันออก คนที่ถูกฝังในเนินดินหลุมฝังศพทางตะวันตกมีร่างกายที่สูงกว่าและมีการเสื่อมผุของเคลือบฟันน้อยกว่าคนที่ฝังที่เนินดินทางตะวันออก และแม้ว่าการเสื่อมผุของเคลือบฟันและอัตราการเสียชีวิตของผู้หญิงลดลงเมื่อเปรียบเทียบกับผู้ชาย แต่ผู้หญิงก็มีส่วนสูงเฉลี่ยลดลง และมีอัตราการเสียชีวิตของทารกในครรภ์สูงขึ้นตามช่วงเวลาซึ่งอาจจะแสดงว่าผู้หญิงมีชีวิตความตึงเครียด และคนที่มีสถานภาพทางสังคมต่ำก็มีความตึงเครียดมากกว่าคนที่มีสภาพทางสังคมที่สูงด้วย หลักฐานประเภทโบราณวัตถุและจารึกโบราณสนับสนุนว่าความแตกต่างทางสถานภาพทางสังคมและเพศที่แหล่งโบราณคดีโนนบ้านจากเกิดขึ้นและดำรงอยู่ดังจะสะท้อนให้เห็นจากการฝังศพในที่อยู่อาศัยและการเข้ามาของระบบความเชื่อทางศาสนาอันใหม่