Grain size analysis of Turonian Amasiri Sandstone in southern Benue Trough has been undertaken to determine the controversial depositional environment of the formation. The formation was first studied on outcrops and 26 representative samples were collected and subjected to particle size analysis in line with standard procedures for dry sieving. Various methods of environmental interpretation of grain size distribution data were applied to constrain the depositional of the sandstones. The result indicates that the sands are medium and coarse-grained with mean size ranging from 0.15 to 1.87φ and averaging 0.96 φ. The sandstones are moderately to poorly sorted with standard deviation values ranging from 0.72 to 1.38 φ and averaging 1.07 φ. They exhibit a wide range of distribution from strongly coarse skewed to strongly fine skewed with skewness values ranging from -2.31 to 1.52 φ and averaging -0.04 φ but indicate a narrow range of kurtosis from mesokurtic to leptokurtic distribution with values ranging from 0.99 to 3.49 φ and an average of 2.06 φ. The sediments have bimodal with minor polymodal and unimodal distribution with primary modal size of 1.2 φ. The bivariate plots of size statistical parameters indicate fluvial environment of deposition. However, linear discriminant function analysis and the interpretations of log-probability plots indicate deposition in a fluvial, beach, and shallow marine settings, and thus suggesting a possible deposition in high-energy transitional environment. The C-M pattern of the samples indicates that sediments were transported mainly by rolling and suspension with subordinate fractions moved by rolling as well as suspension. Thus, it is deduced that Amasiri Sandstone was deposited in fluvial, beach, and agitated shallow marine environments.