Line Transect Research Articles

Avian species and diversity on the campus of Universitas Sumatera Utara, Medan City

Universitas Sumatera Utara (USU) is strategically situated for conducting investigations into the biodiversity of flora and fauna, serving as a source of genetic resources for educational purposes. USU, as a green campus, plays a multifunctional role in determining, protecting, and disseminating information regarding biodiversity, including avian diversity, to support environmentally sustainable development. The USU campus area essentially serves as an urban zone with a focus on species diversity and conservation, making it a key aspect of management. This study aims to identify bird species and diversity on the campus and analyze the diversity index and evenness of bird species on the campus. The methods employed involve direct observations using the line transect method combined with the point count method. We documented a total of 36 bird species, encompassing 22 families. The Avian Diversity Index value on the campus was calculated as 3.117, and the Avian Evenness Index was 0.870. The most prevalent families were Columbidae and Cisticolidae, constituting 3.8% of the total.

Communities Interaction with Sumatran Tiger (Panthera tigris sumatrae) in the Buffer Zone of Batang Gadis National Park

Bukit Barisan Grand Forest Park Forest is a forest area formed from forests with protected forest status. In this forest area there is a main road connecting the cities of Medan and Berastagi, which is a tourist destination for people in North Sumatra. Therefore this area is very unique to study but is also vulnerable to logging disturbances, etc. Apart from that, tourist activities also put pressure on this area, especially during holiday periods. The aim of this research is to determine the composition of stands and the carbon content stored in Dolay Rayat Village, Karo Regency. The method used is a combination of stripes and plotted lines where the plots are placed using systematic sampling with random start. Meanwhile, allometric equations are used to estimate carbon stocks. The research used 50 sample plots (2 Ha) arranged in 10 transect lines. The results of the vegetation analysis that has been carried out show that there are 29 types of standing vegetation. The dominant species found at the seedling level are Eurya accuminata (IVI: 30.05), saplings are Altingia excelsa (IVI: 34.44), poles are Lithocarpus edulis (IVI: 44.61) and trees are Lithocarpus edulis (IVI: 30.82). This forest has moderate species diversity with an H’ value ranging from 2.41-2.76. Carbon reserves stored in forest areas in the administrative area of Dolat Rayat Village are quite high, namely 14,639.04 tons.

Forest composition and carbon reserves in Bukit Barisan Forest Park, Dolat Rayat Village, Karo District

Bukit Barisan Grand Forest Park Forest is a forest area formed from forests with protected forest status. In this forest area there is a main road connecting the cities of Medan and Berastagi, which is a tourist destination for people in North Sumatra. Therefore this area is very unique to study but is also vulnerable to logging disturbances, etc. Apart from that, tourist activities also put pressure on this area, especially during holiday periods. The aim of this research is to determine the composition of stands and the carbon content stored in Dolay Rayat Village, Karo Regency. The method used is a combination of stripes and plotted lines where the plots are placed using systematic sampling with random start. Meanwhile, allometric equations are used to estimate carbon stocks. The research used 50 sample plots (2 Ha) arranged in 10 transect lines. The results of the vegetation analysis that has been carried out show that there are 29 types of standing vegetation. The dominant species found at the seedling level are Eurya accuminata (IVI: 30.05), saplings are Altingia excelsa (IVI: 34.44), poles are Lithocarpus edulis (IVI: 44.61) and trees are Lithocarpus edulis (IVI: 30.82). This forest has moderate species diversity with an H’ value ranging from 2.41-2.76. Carbon reserves stored in forest areas in the administrative area of Dolat Rayat Village are quite high, namely 14,639.04 tons.

Density and Distribution Pattern of Lola Snail (Trocus niloticus) in the Coastal Waters of Malaku Village, Central Maluku

The lola snail (Trochus niloticus) is a species of sea snail that has important economic value. This research aims to determine the density and distribution patterns of lola snails in the coastal waters of Malaku Village, Central Maluku. Sampling used a stratified random sampling method with line transects, the research location was divided into 3 stations. Stations I and III have plank coral substrates and dead coral fragments, while station II has rocky substrates and coral flats. The results of the research found that the total number of lola snail individuals found at the research location was 110 individuals spread across 3 research stations. The maximum size of lola snails found was 99.41 mm in diameter and the minimum size found was 47.18 mm in diameter. The highest density of Lola snails at each research station was 0.08 ind/m2 and the lowest was 0.05 ind/m2. The distribution pattern of Lola snails at the research location is a clustered pattern.

Capung (Ordo: Odonata) di Kawasan Reklamasi Bekas Tambang Timah, Belinyu, Bangka

Dragonflies are bioindicator insects that are known to be sensitive to the environment and their existence is influenced by habitat conditions. The potential of dragonflies as a bioindicator for the success of reclamation efforts for former tin mining on Bangka Island needs to be studied. This research aims to record the diversity of dragonflies and damselflies in reclaimed land covering an area of ±15.25 ha and aged ±16 years as one component to enrich the criteria for assessing the success of revegetation of ex-tin mining land. This research was conducted in the rainy season, January-March 2023. For three weeks, Schnabel's visual encounter survey (VES) and capture-mark-release-recapture (CMRR) methods were chosen to observe five transect lines, respectively non-revegetation areas, horticultural and fruit revegetation areas, oil palm revegetation areas, kolong with tilapia fish, and kolong with human activities. This research recorded 19 species of dragonflies and 6 species of damselflies, or around 6.5% of the dragonflies and 2.0% of the damselfly species on the island of Sumatra. The most dominant species are Neurothemis fluctuans and Ceriagrion cerinorubellum. The number of species and number of individual dragonflies is thought to be related to the age of reclamation, and has the potential to be an indicator of the success of ex-tin mining reclamation land. Keywords:“Area Type”; “Bangka”; “Bioindicator”; “Dragonfly”; “Ex-Mined Land Reclamation”

Seagrass growth at different turbidity levels

Seagrass beds are highly productive ecosystems and have ecosystem services in the coastal zone but are continuously declining globally. Mainly due to anthropogenic activities that reduce the amount of light entering the waters and reaching seagrasses, such as dredging, reclamation, sedimentation, and eutrophication. This research was conducted to determine the relationship between turbidity level and seagrass composition and morphology. The observations were conducted in November 2016 in Pangkep district on Sagara Island and Makassar City on Barrang Lompo and Lae-lae Island, South Sulawesi Province. The observation stations are determined based on the level of turbidity conditions. Sagara Island, Barrang Lompo, and Lae-lae are areas that are considered to represent turbid waters, by laying a line transect (roll meter) perpendicular to the coast and doing it 3 times. The data collected in this study related to seagrass conditions such as seagrass composition and seagrass morphometrics. Variables observed in seagrass leaf morphometric observations were leaf surface area and leaf width, leaf thickness, and distance between internodes. In addition, other data collected is environmental parameter data, such as currents, TSS, PAR, temperature, and salinity. One-way ANOVA analysis showed that there was a difference in turbidity levels between Barrang Lompo Island, Sagara Island, and Lae-lae Island with a significance value of 0.000. The types of seagrasses found on Barrang Lompo with light availability of 1735.23 µmol/m²/s are Enhalus acoroides, Cymodocea rotundata, Halodule uninervis, Halophila ovalis, Thalassia hemprichii. On P. Sagara with light availability of 170.01 µmol/m²/s, E. acoroides, T. hemprichii, Cymodocea serrulata, H. uninervis, and H. ovalis were found. Whereas in P. Lae-lae only E. acoroides was found. The results showed that the level of turbidity has an influence on seagrass morphometrics, namely leaf ratio and leaf thickness of E. acoroides species, and internode distance in H. uninervis species. Keywords:Availability of lightMorphometricsPARSeagrass bedsTurbidity

Stand growth, Biomass and Carbon sequestration potentials of Parkia biglobosa (jacq.) Bench plantation in South-Western Nigeria

This study assessed tree growth variables, above (AGB), below ground biomass (BGB) and total carbon content (TC) sequestered by Parkia biglobosa (Jacq.) Bench. Plantation in Wasangare, Oyo State using non-destructive ground base survey. Tree growth data (Diameter at breast height, Dbh and Tree height, Th) were collected using lacer ace hypsometer and diameter girth tape from 20 temporary sampling plots of size 25 m X 25 m established through systematic transect lines. Diameter size classes (DSC) for the plantation was examined, carbon stock for each DSC was also determine while basal area (m2 ha-1), volume (m3 ha-1), Biomass (Mg ha-1) and Carbon (Mg ha-1) were also estimated. Results showed mean Dbh of 18.7 + 0.25 cm with 8.14 + 0.10 m, 0.033 + 0.00 m2 ha-1 and 0.320 + 0.01 m3 ha-1 for tree height, basal area and volume respectively. AGB and BGB were 10.877 + 0.39 Mgha-1 and 2.175 + 0.08 Mgha-1 respectively while TC was 6.527 + 0.24 Mgha-1. The percentage carbon stock proportion for each DSC revealed class size 25-29-9 cm (19.02%) as the highest while the least proportion was observed in less than 5 cm class with 0.04% of carbon. The DSC showed majority of the tree Dbh in lower Dbh classes with fewer trees in higher classes forming almost a normal bell shape. The study provides information that can help the management in planning silvicultural activities and selective removal from the stand (harvesting schedule). Tree Dbh, height, basal area, volume and biomass are the determinant characteristics for forest carbon assessment. In conclusion, the plantation actively sequesters carbon showing potentials for indigenous trees in climate change mitigation.

Density and abundance estimation of West Indian manatee, Trichechus manatus, between the states of Ceará and Piauí, Northeast Brazil, using active acoustics

The West Indian manatee is one of the most threatened species in Brazil. The species has currently a patchy distribution from the state of Alagoas to the state of Amapá. The difficulty of observing manatees, especially in estuarine waters, is a challenge for conservation. Therefore, it is necessary to use new methodologies and technologies to solve manatee detection problems in their natural habitats. The goal of this study was to use an active acoustic method of detection to estimate manatee density and abundance in the estuarine complex of the Timonha and Ubatuba rivers, between the states of Ceará and Piauí, northeast Brazil. Data collection was conducted from a wooden boat with an outboard motor using a side-scan sonar. Sonar images were collected along line transects in three regions within the study area. Manatee abundance was calculated using distance sampling (Distance 6.0 software). There were 1,396 transection lines that totaled 863.6 km traveled in the study area. The observed general encounter rate was 0.089 group detected per kilometer traveled. The best detection model was the hazard-rate with no adjustment terms, resulting in a detection probability of 33.7%, and an estimated density for the entire study area of 9.19 manatees per km2. Abundance of manatees in the estuary was estimated to be 37 animals (CV% = 30.2, 95% CI = 21 - 66). The methodology using side-scan sonar proposed here was successful in the detection of manatees in the study area and in defining parameters for its use to estimate the population size using the distance sampling method. This study provides an estimate of manatee density and abundance in a key estuarine system along the northeast coast of Brazil, which can serve as a baseline for future studies and aid in the development of conservation strategies for the species. This is the first time this methodological approach has been used for manatee detection and abundance estimation in Brazil. We recommend the use of side-scan sonar in future West Indian manatee research.

Community structures of bivalves in the coastal area of Lungkap Village, Pinolosian, Bolaang Mongondow Selatan District

This research aims to identify the structure of the bivalve community on the coast of Langkat Village, Pinolosian, South Bolaang Mongondow District. Sampling was carried out from December 2020 to January 2021 at three stations, namely seagrass beds (station I), tourist attractions (Station II), and mangroves (Station III), using the quadrant transect method, where each observation station made a line transect from the mainland towards the sea. Line transects are placed at each station, while the distance between line transects is 50 m. Observation results show that the bivalves at the research location consist of five families (Arcidae, Asteridae, Cyprinidae, Cardiidae, Ostreidae) an six types of bivalves, where the diversity index is low and the uniformity index is moderate, indicating that the distribution of each type is relatively even.Keywords: Bivalve; coastal; community structure; Lungkap

Community Structure Of Seagrass In Southeast Waters Cempedak Island West Kalimantan

Cempedak Island is one of the Coastal and Small Islands Conservation Areas (KKP3K) in West Kalimantan has various marine biological resources and important ecosystems, including seagrass. Seagrass is used as the main food source for dugong, potential habitat for various types of organisms, and potential to be developed as marine ecotourism. On the other hand, seagrass ecosystems faced threats, such as high sedimentation and the influence of anthropogenic activities. It is unknown about the current status of seagrass on Cempedak Island. This research aimed to analyze the structure of seagrass in the southeastern waters of Cempedak Island. Seagrass data collection was carried out in the tidal zone using line transect of 70 m and a quadratic transect of 1x1 m2. Data collection on seagrass and water parameters was carried out in October 2023. Determination of research sites was done using an explorative method based on the presence of seagrass. The seagrass found consisted of 4 species, namely Enhalus acoroides, Cymodocea rotundata, Halodule pinifolia, and Halophila ovalis. Seagrass cover ranged from 9.17–16.74%, with an abundance of 1.4–373.3 ind/m2. The diversity index (H') is in the low to medium category, the uniformity index (E) is in the medium category and the dominance index (C) is in the low category. Conditions of water quality parameters were into the optimal range to support seagrass life.

Long monitoring of Birds of Elssuki Area. Sinnar State, Sudan

This study aims to make a database of the birds in the area of Elssuki, Sinnar state - Sudan. The period of study spanning from 2008 to 2023 with a mix of methods used to identify bird species in many sites in the locality, these methods include road count, line transects, and direct count besides registering every strange, rare, or unusual single species seen in the area. All these methods are used by different researchers and applied in such studies in Sudan. The study revealed that the area is one of the important areas enriched of birds in Sinnar state of 19 orders 53 families. The total number of species is 129 species. It included all birds; water birds, tree birds, diving birds, dabbling birds, swimming birds, small waders, and passerines which the most. The study concluded that there is a need for comprehensive and regular studies and short and long-term monitoring to identify, classify, and establish a database for Sudan Birds Atlas.

Assessment of the global population size of the Mongolian gazelle Procapra gutturosa

Abstract The Mongolian gazelle Procapra gutturosa is a wild ungulate ubiquitous across the largest remaining temperate grasslands of Mongolia, Russia and China. The species is nomadic and ranges over long distances, resulting in widely fluctuating abundance in any given location. Therefore, a comprehensive and range-wide survey is required to accurately estimate its global population size, but challenges are posed by the expansive geographical distribution and the political boundaries across the species’ vast range. To obtain an estimate of the total population, we compiled data from recent range-wide surveys. During 2019–2020, we estimated the population size in Mongolia by conducting line transect distance surveys and total counts, and by deriving numerical predictions for unsurveyed areas through data analysis. The gazelle's population in Russia was surveyed in 2020 across its summer range using simultaneous counts, transect surveys and expert knowledge. The distance sampling surveys in Mongolia revealed that slightly more than half of the gazelles along the transects were detected. Our assessment of the gazelle population, although probably an underestimate, suggests there are c. 2.14 million individuals in Mongolia and c. 30,000 in Russia. These results confirm that the Mongolian gazelle is the most abundant nomadic ungulate in the open plains across its range. However, to obtain more accurate estimates across all range states and effectively monitor the gazelle's population status, it is essential to implement standardized survey protocols that correct for imperfect detection. At present, the management of the Mongolian gazelle is inadequate, as there is a lack of regular monitoring to identify any adverse population changes that could necessitate conservation interventions.

Population of The Javan Surili Langur (Presbytis Comata) in The Taman Safari Indonesia Bogor Forest Area

Taman Safari Indonesia Bogor is a buffer zone for the Taman Nasional Gunung Gede Pangrango conservation forest area and is one of the habitats for the Javan surili langurs, but there is no research data on wild Javan surili langur populations. The object of research is to find out how many populations of Javan surili langurs are in the TSI Bogor forest area. The research method used the line transect method by collecting data on the number of individuals, the number of groups, the sex ratio, and the age structure. The research was conducted in four observation areas with varying lengths, observations were made at (06.00 - 17.00 WIB), and four repetitions were carried out on each observation path, the results showed that there were 16 Javan surili langurs individuals divided into one group, and the estimated population density was 0.06 individual/ha. The sex diversity of male and female surili langurs in the TSI Bogor forest area is 1:6 where the female Javan surili langurs are more than the male Javan surili langurs and for the age structure the largest Javan surili langurs are in the adult age class with a percentage of 43.75%, juvenile 31.25%, and the percentage of infant is 25%.

Plant Community Composition and Structural Pattern Dynamics in Robe-Raya Natural Forest, Southeast Ethiopia

Due to its fortuitous mix of geography, terrain, and geology, Ethiopia is the home of unique assemblages of rich biodiversity. However, this impressive biological diversity is increasingly threatened by the combined effects of different drivers before they are sufficiently investigated. The present work was carried out in Robe-Raya Natural Forest, located in Southeast Ethiopia, with the intention of examining plant community formation and structural dynamics of the forest species. Sixty (20 m × 20 m) quadrats were placed at 100 m distance along eleven east-west directed transect lines systematically. In order to gather juvenile’s data, five subquadrats (2 m × 2 m) were established within the main quadrat, distributed at each corner and middle. In each quadrat, all woody species were recorded and counted; diameter (DBH) and height were measured using tape meter and a hypsometer, respectively, and cover abundance was recorded (in %). Cluster analysis was computed using R-Package to map-out the community types. Species diversity and composition among community types were computed using the Shannon-Wiener index and Sorenson’s coefficient, respectively. Frequency, density, height, DBH, basal area, and IVI were used to analyze structural dynamics. Age-class density ratios were used to examine the regeneration status. Ninety-four woody plant species belonging to 39 families were documented. Asteraceae was the most species-rich family (10 species). The common growth form was shrubs (44.7%) followed by trees (41.5%). Cluster analysis produced four community types. In total, the species diversity and evenness were 3.75 and 0.88, respectively. The forest density and basal area were 1183.3 stems/ha and 57.52 m2·ha−1, respectively. Structural dynamics analyses demonstrated that the forest was composed of, largely, young trees and shrubs and under fair regeneration status. Certain species that have been identified to have low IVI and poor regeneration status should be prioritized for conservation.

Woody Species Composition, Structure, and Status of Regeneration in Pugnido Forest, Gambella Region, Western Ethiopia.

Ethiopia is a biodiversity hotspot area with a high concentration of plant species that play countless roles in ecosystem resilience and mitigating the effects of climate change. However, forest resources are degrading at an alarming rate due to different anthropogenic factors. Pugnido Forest, which is among Ethiopia's greatest woodland sections, also faced a similar challenge. Thus, this study was intended to assess the composition, structure, and regeneration status of woody plant species in Pugnido Forest, West Ethiopia. Eighty main plots sized 20 × 20 m were sampled systematically on six east-west-oriented line transects, which are parallel to each other and 500 m apart. At the four corners and the center of the main plots, five subplots sized 2 m × 2 m were laid to collect seedling and sapling information. Individual woody species were identified and then recorded per plot. The forest structure and regeneration status data were recorded and analyzed using structural parameters. Communities were identified via cluster analysis. A comparison of diversity and composition among communities was made using the Shannon-Wiener diversity and Sorensen's similarity coefficients, respectively. A total of 106 woody species included in 40 families were documented. Fabaceae stands first in the species-rich family (23.6%), and Moraceae stands second (8.5%). Overall Shannon-Wiener diversity and evenness of species were 4.41 and 0.93, respectively. Four communities were generated from cluster analysis. Sorensen's similarity value among communities ranged from 42% to 59%. The forest's density and basal area were 5298.8 stems·ha-1 and 82.5 m2·ha-1, respectively. The trend in frequency and density classes showed a decreasing number of species towards higher classes. Diameter at breast height (DBH), height, and basal area analyses revealed a normal J-shaped pattern. Several species had a smaller importance value index (IVI) value. Density ratios of juveniles to adult plants indicated good regeneration status. Species with small IVI values and few or no seedlings require conservation priority. Top priority must be given to conserving species with low IVI values and few or no seedlings.

Insights into key threats and conservation status of the river lapwing, Vanellus duvaucelii (Lesson, 1826) in Northern India

Abstract The river lapwings are inhabitant of river banks with sand or gravel bars and river islands. In this study, we investigated vital threats (natural and anthropogenic) and the conservation status of river lapwing in the riverine ecosystem of Northern India. In this regard, we frequently visited selected study sites along the banks of the river Ganges in the district of Raebareli (Uttar Pradesh), India, from January 2016 to December 2019. To estimate perceived threats for river lapwing, we developed a questionnaire and collected threat scores. The line transect method was used to estimate the density of river lapwing. Predation and farming activities were the most potent threats influencing the survival and abundance of river lapwing. River lapwings were primarily observed at open unvegetated river banks and open unvegetated islands. They were seen in significant density near the water in the breeding season. We concluded that the population of river lapwing is relatively stable in the Gangetic plains of Northern India. However, it is declining in other parts of the world, for example, in Southern Laos. Though it is a relatively common species, robust scientific information about its population and habitat relation is mainly absent. Therefore, accurate counts from other parts of the world are needed to place the estimate for river lapwing into a comprehensible, inclusive perspective. Furthermore, detailed information on habitat relationships is also necessary to develop conservation strategies.

Assessment of Avian Richness in Nature Park, Kolkata, West Bengal, India

This study is a preliminary inventory of the avifauna diversity in Nature Park, situated in the south western periphery of Kolkata within the state of West Bengal, India. A rigorous study of avifauna was carried out from March 2022 to February 2024. Basic line transects and point count methods were used in this study. A total of 108 bird species taxonomically belonging to 79 genera, 41 families and 12 orders were recorded from the study site. Passeriformes was the most predominant order with 48 species belonging to 21 families. Ardeidae with 11 species was the most dominant family. Maximum numbers of species were noted in genus Ardea. One vulnerable and one near threatened bird species was recorded from the study site regarding IUCN status.

Horse populations are severely underestimated in a region at risk of Hendra virus spillover.

To identify the size and distribution of the horse population in the Northern Rivers Region of NSW, including changes from 2007 to 2021, to better understand populations at risk of Hendra virus transmission. Census data from the 2007 Equine Influenza (EI) outbreak were compared with data collected annually by New South Wales Local Land Services (LLS) (2011-2021), and with field observations via road line transects (2021). The horse populations reported to LLS in 2011 (3000 horses; 0.77 horses/km2) was 145% larger than that reported during the EI outbreak in 2007 (1225 horses; 0.32 horses/km2). This was inconsistent with the 6% increase in horses recorded from 2011 to 2020 within the longitudinal LLS dataset. Linear modelling suggested the true horse population of this region in 2007 was at least double that reported at the time. Distance sampling in 2021 estimated the region's population at 10,185 horses (3.89 per km2; 95% CI = 4854-21,372). Field sampling and modelling identified higher horse densities in rural cropland, with the percentage of conservation land, modified grazing, and rural residential land identified as the best predictors of horse densities. Data from the 2007 EI outbreak no longer correlates to the current horse population in size or distribution and was likely not a true representation at the time. Current LLS data also likely underestimates horse populations. Ongoing efforts to further quantify and map horse populations in Australia are important for estimating and managing the risk of equine zoonoses.

Carbon stock of shatto forest along altitudinal gradients in masha district, southwest ethiopia: a contribution to climate change mitigation

Forests play a significant role in climate change mitigation through carbon sequestration from the atmosphere and retaining carbon in above and belowground biomass of trees, litter biomass and soil. However, there is little empirical data on the potential carbon stock of Ethiopia’s various forests. Therefore, this study was carried out to estimate the carbon stock potential of Shatto Forest along altitudinal gradients and its contribution to climate change mitigation in Masha district, southwest Ethiopia. Using a systematic sampling design, a total of 30 plots measuring 20 x 20 m were established at 200 m intervals along seven transect lines in order to inventory the woody species. Within the main plot, a 1 m x 1 m sub-plot was used to collect soil samples and litter biomass. The biomass carbon stock was estimated using allometric equations. Results revealed that the mean total carbon stock of the study forest was 714.4 ton/ha, of which 337.38ton/ha, 67.48 ton/ha, 0.79 ton/ha and 308.75 ton/ha were stored in the aboveground carbon, belowground carbon, leaf litter carbon and soil organic carbon, respectively. The mean total CO2 equivalent of the study forest was also 2621.85 ton/ha. Along the altitudinal gradients of the study forest, the total carbon stocks contributed by the forest varied significantly at (p < 0.05), with the largest stock found at lower elevation and the lowest stock found at higher elevation. Based on the study findings, it is concluded that the forest under investigation possessed a substantial carbon stock capacity, allowing it to sequester a significant amount of CO2 and mitigate climate change while offering other ecosystem services. Thus, in order to optimize the forest’s capacity to store carbon and obtain the financial benefits of CO2 mitigation, reduced emission from deforestation and degradation (REDD+) program in the area should be integrated with carbon sequestration.

Identification of biotic and abiotic factors coal mine overburden on Warukin rock formation of South Kalimantan

Overburden (the dumping of mine tailings and other reject materials) from the Warukin geological formation is one of the potential acid-forming (PAF) of 33.17%. Mining can cause overburden to be exposed and affect biotic and abiotic conditions. The study aimed to analyze the biotic and abiotic factors of three different age overburdens from the Warukin Formation, South Kalimantan. Biotic factor measurements included vegetation sampling using line transect, soil insects using pitfall traps, and microbial using total plate count. Abiotic factor measurements included air temperature and humidity, light intensity, temperature, moisture, and pH of the substrate (n=3), and chemical overburden. Identification of plants and insect species using information from locals, identification books, and websites. Plant species diversity was analyzed using the Shannon-Wiener Index. Dominance species were analyzed using the Important Value Index (IVI). The relation of overburden age on biotic and abiotic factors was analyzed multivariate with Non-Metric Multidimensional Scaling (NMDS). The results showed that there are 102 species of plants and 11 species of soil insects. The numbers of fungi and bacteria are 10-4 CFU g-1 and 10-3 CFU g-1, respectively. Plant species diversity is classified as medium (H'= 2.34; 2.50 and 2.82). Changes in plant composition occurred as the microclimate and substrate nutrition improved. The organic matter increased over time. Humidity and light intensity influence the presence and composition of organisms, while pH values affect the adaptability of plants, insects, and microbes. Heavy metal concentrations have increased due to the physicochemical properties of the Warukin formation overburden.