Event Abstract Back to Event Laser scanning microscope for scanning along 3D trajectories with 5 ms time resolution Gergely Katona1*, Balazs Chiovini1, Szilveszter E. Vizi1 and Balazs Rozsa1 1 Institute of Experimental Medicine Hungarian Academy of Sciences, Hungary We developed a new scanning strategy and a new 3D two-photon microscope for dendritic imaging with 5 ms time resolution. We use our multiple line scan technique (Differential distribution of NCX1 contributes to spine-dendrite compartmentalization in CA1 pyramidal cells (2007) Proc. Natl. Acad. Sci. USA, 10.1073) with the combination of two z-scanning methods. This combination results in movement typical for a roller coaster. Our technique provides 20 µm scanning range along axis z when it is used with 6 ms repetition rate. The spatial resolution (namely the mean half width of the point spread function) is better than 500 nm. We optimized our detectors for scattered photons; therefore, our system provides an excellent signal to noise ratio when compared to commercially available systems. Using our method, we measured the mechanism of the initiation and spread of dendritic regenerative activity (dendritic spikes) induced by focal electric stimulation. Our technique revealed a new form of dendritic spikes and synaptic integration in hippocampal CA1 stratum radiatum interneurons. Conference: 12th Meeting of the Hungarian Neuroscience Society, Budapest, Hungary, 22 Jan - 24 Jan, 2009. Presentation Type: Poster Presentation Topic: Research on the cerebral cortex and related structures Citation: Katona G, Chiovini B, Vizi SE and Rozsa B (2009). Laser scanning microscope for scanning along 3D trajectories with 5 ms time resolution. Front. Syst. Neurosci. Conference Abstract: 12th Meeting of the Hungarian Neuroscience Society. doi: 10.3389/conf.neuro.01.2009.04.204 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 06 Mar 2009; Published Online: 06 Mar 2009. * Correspondence: Gergely Katona, Institute of Experimental Medicine Hungarian Academy of Sciences, Budapest, Hungary, katonag@koki.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Gergely Katona Balazs Chiovini Szilveszter E Vizi Balazs Rozsa Google Gergely Katona Balazs Chiovini Szilveszter E Vizi Balazs Rozsa Google Scholar Gergely Katona Balazs Chiovini Szilveszter E Vizi Balazs Rozsa PubMed Gergely Katona Balazs Chiovini Szilveszter E Vizi Balazs Rozsa Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.
Read full abstract