Limpopo Province Research Articles

Phylogenetic position of Pratylenchus sp. (Nematoda: Pratylenchidae) associated with grasslands using 28S rDNA from South Africa

Plant-parasitic nematodes are the main nematodes that live in the soil, causing yield loss in various crops. As endoparasites, Pratylenchus species can reduce the yield of grass and other plants by feeding on their roots. Because of its parasitic nature on plants, this species requires careful identification; in South Africa's Limpopo Province, grasslands are among the most vital agricultural resources. Therefore, this molecular study was conducted in 2023 at Limpopo University to identify the nematode from South Africa's soils using 28S rDNA marker. The recovered nematode was extracted using the tray method, and then its DNA was extracted using the chelex method. The nematode was identified as Pratylenchus. Afterward, 28S rDNA was amplified using specific primers to identify the nematode at the molecular level. The Nblast analysis based on the large subunit ribosomal DNA showed South African Pratylenchus had 99% similarity (OQ343703) with the South African population. Phylogenetic analysis using maximum likelihood placed this species with those molecularly identified as Pratylenchus in the same clade with highly supported (100) bootstrap values. In conclusion, this species was identified using 28S rDNA; however, other rDNA markers, such as ITS rDNA and mtDNA is recommended for a better understanding of Pratylenchus phylogeny.

Reinstatement of Aloe mutans (Asphodelaceae subfam. Alooideae), a distinctive, endemic, maculate aloe from the central Limpopo province of South Africa

Until 1987, Aloe mutans (Asphodelaceae subfam. Alooideae) was accepted as a distinctive species of maculate aloe from South Africa’s Limpopo province. However, this species thereafter often has been included in the synonymy of A. greatheadii var. davyana. Recently, A. davyana was separated from A. greatheadii at the rank of species, which requires reconsideration of the taxonomic status of A. mutans. It is shown that A. mutans differs from A. davyana in several vegetative and reproductive morphological characters so warranting its recognition at the rank of species. Aloe mutans is therefore here reinstated as an accepted species. Both A. mutans and A. davyana are illustrated and an amplified description is provided for A. mutans.

Bergbambos tessellata (Poaceae) endemic to southern Africa: new record from the Limpopo Province, South Africa

Bergbambos is a temperate C3 bamboo grass genus, consisting of one species found mainly along southern African mountain ranges (Temperate Afromontane Region). We document here a new record of Bergbambos tessellata found in the Thabazimbi area along Hamerkop Street near the fence of an old nursery (non-functional). The specimen was identified at the National Herbarium (PRE) using a light microscope and compared with other herbarium specimens or materials, collected from other South African provinces. This discovery is accompanied by diagnostic features for precise species differentiation, as well as illustrations facilitating visual distinction. Notably, Bergbambos tessellata exhibits an unusual geographical range in the Waterberg Biosphere region within the valley. This discovery not only enriches our comprehension of bamboo dispersion dynamics but also engenders a heightened impetus for in-depth investigation into the various factors that underlie its restricted presence within this specific ecological niche. The documentation of this species therefore holds considerable importance in formulating effective conservation strategies and promoting sustainable management practices.

The effects of habitat and climate on tick abundance and diversity on a game reserve in the Limpopo Province, South Africa

ABSTRACT The livelihoods of local farmers and game ranchers in Africa – and the communities these businesses support – are threatened by tick-borne diseases that kill and weaken livestock. Understanding the local ecology of ticks may inform cost-effective and sustainable techniques for controlling tick populations. Our objective was to describe differences in tick abundance, species richness, and community structure in three different habitats from June-July 2022 on a game reserve in the Limpopo Province of South Africa. Ticks were sampled using the drag method, in 54 30 m × 5 m transects evenly distributed across bushveld, long grass, and short grass habitats. Total tick abundance and species richness were significantly greater in long grass than in bushveld and short grass. Although there were no significant differences among the habitats in Simpson’s diversity, the composition of the communities did vary significantly, with the relative abundances of Rhipicephalus appendiculatus and R. evertsi evertsi peaking in long grass, Amblyomma hebraeum peaking in bushveld, and R. decoloratus peaking in short grass. Differences in habitat suitability for ticks and habitat use by ungulate hosts may explain the significant effect of habitat on tick abundance, species richness, and community structure.

Prevalence and Risk Factors Associated with HIV Infection among Pregnant Antenatal Care Attendees in Limpopo Province, South Africa

Early screening for HIV infection provides an opportunity for mother-to-child transmission and optimizes the care of HIV-infected mothers and unborn babies to improve clinical outcomes. This study aimed to determine the prevalence and the risk factors associated with HIV infection among pregnant women attending antenatal care at the District Hospital and its feeder community health center of the Limpopo Province (LP), South Africa. The study was a cross-sectional descriptive study carried out over 2-months from 01 May 2019 to 30 June 2019. A consecutive sample of pregnant women who attended antenatal care during the study period was asked to participate. In total, 211 pregnant women participated in this study. Their mean age was 28.4±5.7 years, ranging from 18 to 41 years. More than half (56.4%) were aged <30 years old, 51.7% had secondary education, 71.1% were unmarried, and 72.0% were unemployed. The majority (66.4%) of pregnant women had multiple pregnancies and 70.6% were in the third trimester. Few (0.95%, n=2) had a history of alcohol use. The HIV prevalence was 15.2%, and significantly high in illiterate, elementary school-educated and multiparous women. The HIV infection rate in this setting is relatively associated with the level of education and parity. The social risk factors of health in each municipality should be considered when local health authorities implement policies. Women should be continually provided with health education about modes of transmission of HIV prevention particularly those with lower levels of education and reproductive age.

Biomonitoring of bisphenol A (BPA) and bisphenol analogues in human milk from South Africa and Canada using a modified QuEChERS extraction method

A sensitive modified QuEChERS extraction method was developed to assess the levels of free and conjugated bisphenols (BPs) in human milk collected between 2018 and 2019 from two regions of South Africa (the Limpopo Province Vhembe district, n = 194; Pretoria, n = 193) and Canada (Montreal, n = 207). Total BPA (free and conjugated) and BPS were the predominant bisphenols detected in samples from Vhembe and Pretoria, whereas total BPS was the predominant bisphenol detected in Montreal samples. The levels of total BPA in samples from Vhembe and Pretoria ranged between < MDL-18.61 and <MDL-19.38 ng/mL, with medians of 1.03 ng/mL and 0.69 ng/mL and detection frequencies of 73% and 68%, respectively. The speciation analysis of BPA revealed a predominantly conjugated form in South African samples. In contrast, total BPA was detected in only one milk sample from Montreal. Total BPS levels were lower than BPA in South Africa, with detection frequencies of 57% and 21% in Vhembe and Pretoria, respectively. In contrast, total BPS was the major BP detected (42%) in Montreal (up to 4.42 ng/mL). BPAF was found exclusively in South Africa, with detection frequencies for total BPAF of 40% and 9% in Vhembe (<MDL-12.41 ng/mL) and Pretoria (<MDL-0.11 ng/mL), respectively. To our knowledge, this is one of the first studies to detect bisphenols in human milk from data-scarce countries such as South Africa and to highlight the notable disparities in the types and levels of bisphenols detected across two distinct countries (Canada and South Africa).

Correction: Shikwambana et al. Enhancing the Resilience and Adaptive Capacity of Smallholder Farmers to Drought in the Limpopo Province, South Africa. Conservation 2022, 2, 435–449

The authors would like to make the following corrections to their published paper [...]

Evaluation of DNA quality and molecular observation of Nile tilapia (Oreochromis niloticus) from Limpopo Province, South Africa

Evaluation of DNA quality and molecular observation of Nile tilapia (Oreochromis niloticus) from Limpopo Province, South Africa

Rural tourism in South Africa: A local economic development strategy

This study aims to assess the potential of rural tourism as a strategy for local economic development in three rural villages in the Thulamela Local Municipality, which falls under the Vhembe District Municipality in the Limpopo province of South Africa. Rural tourism is considered a promising industry for rural development and a popular approach to local economic growth. Sustainable rural tourism is recognised as a crucial component of rural development and is particularly important in developing nations, such as South Africa, where substantial rural populations and limited economic activity exist. The study utilised a qualitative research approach, drawing on an exploratory design and interpretive paradigm. A purposive sampling technique was adopted to select participants. Focus group discussions were conducted with the participants, and content analysis was used to analyse the collected data. The study indicates that rural tourism could effectively promote local economic growth in the three rural villages by providing residents with employment opportunities, thereby reducing poverty rates in rural areas. However, challenges remain, including the lack of funding to support small tourism businesses and the need for human capital development in local communities. The study recommends a gradual approach to knowledge and skills development within local communities to support the business aspects of rural tourism development, as well as the provision of funding assistance.

Tracking enteric pathogen contamination from on-site sanitation facilities to groundwater in selected rural areas of Vhembe District Municipality, Limpopo Province, South Africa

Groundwater is valued as a source of potable water, although it is vulnerable to environmental pollution. The aim of this study was to track enteric pathogen contamination from on-site sanitation (OSS) facilities to 70 household boreholes used by four villages of the Vhembe District Municipality. Two objectives were pursued: to measure the lateral distance between the borehole and the sanitation facilities in household yards, and to track the enteric pathogens. The borehole abstraction point and OSS system distance were determined using a steel measuring tape. Real-time PCR was used to track Shigella flexneri, Salmonella typhimurium, Campylobacter jejuni, and enterotoxigenic Escherichia coli (ETEC) from Wastewater (WW) from domestic septic tank and sludge from pit latrines to boreholes. Escherichia coli was used as an indicator of faecal contamination. Results showed that 25% of households kept a distance of ≥50 m between the OSS facilities and the boreholes. In total, 87.5% of household boreholes in the rainy season and 72.5% in the dry season were contaminated with E. coli and pathogenic bacteria: Shigella flexneri, Salmonella typhimurium, and ETEC. The concentrations of the pathogens ranged from 2.03 to 2.12 LogEGC/100 mL. A very weak (r = −0.093) to moderate (r = −0.541) association was found between pathogens in groundwater and on-site sanitation systems (WW from septic tank and sludge from pit latrine). This suggests that the pathogens were not present in the sanitation compartment when they were found in the groundwater and vice versa. Moreover, a very weak (r = 0.007) to moderate (r = 0.525) association was found between the detected contaminants in groundwater and the lateral distance between the OSS facilities and the boreholes. The pathogens detected in all samples showed consistent concentrations, suggesting potential contamination from OSS systems' waste, possibly in groundwater, indicating potential contamination. The siting of OSS facilities at the yards in this study appeared to have a slight influence on the contaminants detected in groundwater. This study calls for an education program to be implemented by the Water and Sanitation Services Authorities to prevent contamination of groundwater and the risk of waterborne diseases.

IDF23-0012 Anthropometric measurements and attitudes towards diabetes treatment among patients in Limpopo province, South Africa

IDF23-0012 Anthropometric measurements and attitudes towards diabetes treatment among patients in Limpopo province, South Africa

Effect of Sampling Time, Quantification Method, and Tree Sample Pooling on Phytophthora cinnamomi Root Quantities in South African Avocado Orchards.

Phytophthora cinnamomi is a destructive soilborne pathogen causing Phytophthora root rot on avocados worldwide. Little is known about the effect of root sampling time, root quantification method (quantitative real-time PCR [qPCR] versus baiting), and tree sample pooling strategies on the quantification of the pathogen in roots in avocado orchard trees. This was investigated in six avocado orchards in two climatically different production regions (Mooketsi and Letaba) in the Limpopo Province, South Africa, over a 2-year period. Two different tree sample pooling strategies, consisting of either a four-pooled group (four groups each containing five pooled trees) or a single-pooled group (20 trees pooled) per 1 ha, were both shown to be suitable for quantifying P. cinnamomi in tree roots using qPCR or root baiting. P. cinnamomi root quantities from the two tree sample pooling strategies were significantly correlated for both quantification methods. Both quantification methods were suitable for quantifying the pathogen in roots, although qPCR was superior to root baiting at identifying significant differences in P. cinnamomi quantities among root sampling time points. The effect of sampling time was dependent on the investigated year. In 2017, root quantities, which were only evaluated using qPCR, did not reveal a consistent trend of a specific sampling time yielding the highest root quantities for most of the orchards. However, five of the orchards in 2018, based on the qPCR analyses, contained significantly higher P. cinnamomi root quantities in May (late autumn) than in March (early autumn), August (late winter), and October/November (late spring). In 2018, P. cinnamomi root DNA quantities were significantly positively correlated with the number of soil temperature hours at 20 to 24 and 20 to 29°C 2 months preceding the root sampling dates and negatively correlated with the number of hours at 15 to 19°C 2 months preceding root sampling. Our study has identified P. cinnamomi root quantification methods and tree sample pooling strategies, which will be useful for understanding the biology of the pathogen and when disease management strategies should be in place.

Anthropometric status of amateur bodybuilders in Limpopo Province: a profile of Polokwane Municipality athletes

Anthropometric status of amateur bodybuilders in Limpopo Province: a profile of Polokwane Municipality athletes

Gendered Art Entrepreneurship in South Africa: Emerging Artistic Trends Creating Empowerment Opportunities in Limpopo Province

Gendered Art Entrepreneurship in South Africa: Emerging Artistic Trends Creating Empowerment Opportunities in Limpopo Province

First molecular observation on Mylonchulus hawaiiensis from South Africa.

During a survey of soil nematodes in South Africa, a species of predatory nematodes, namely Mylonchulus hawaiiensis was recovered from soil in the Magoebaskloof mountain of Limpopo Province, South Africa. The morphology of the material studied fit well with the previous populations of the same species. A molecular study of 18S rDNA region of M. hawaiiensis indicated a 100% similarity between the South African population and the Japanese population of M. hawaiiensis (AB361438; AB361439; AB361440; AB361442). In addition, phylogenetic analysis placed all M. hawaiiensis in a group with 0.97 posterior probability. Additionally, ITS rDNA of M. hawaiiensis amplified for the first time. However, Principal component analysis (PCA) showed a morphological variation among the different populations of M. hawaiiensis. In addition, haplotype analysis also revealed that the South African population is close to the Japanese population. In conclusion, 18S rDNA was a good marker for detecting M. hawaiiensis. Measurement, photographs, and phylogenetic position of South African M. hawaiinesis are given.

The Biodiversity, Food Security, and Poverty Nexus in the Musina-Makhado Special Economic Zone of South Africa

Wild-sourced foods are entrenched among African women as a survival resource, with the potential to be expanded. Instead of optimising food security, current linear economic growth models in South Africa favour large environment-degrading projects, such as special economic zones and mining, that increase the gap between the wealthy and poor sectors of society. The contentious Chinese-funded Musina–Makhado Special Economic Zone (MMSEZ) planned for the Limpopo province of South Africa is used as a case study in this paper. The MMSEZ case demonstrates the failure of an Environmental Impact Assessment (EIA) to consider alternative land use suited to a biodiverse economy and existing livelihood strategies. Notably, the availability of a popular edible insect, the mopane worm, was not surveyed at a time when it might have been visible. Despite a local and global interest in insect protein, this opportunity was not considered as a means to improve food security and income streams for local unemployed African women.

English first additional language teachers’ attitudes on using e-learning in rural schools in the Vhembe East district in the Limpopo province

The teaching of English First Additional Language (EFAL) in South Africa is gradually changing from the traditional style because of the devastating effects of the COVID-19 pandemic. Efforts are being made to stimulate learners’ interest in learning which include using information communication technology (ICT) in the form of e-learning to enhance understanding. The outbreak of the coronavirus disease (COVID-19) was a major challenge as it compelled teachers to engage in online teaching – even those who were never trained in the use of technology. A qualitative research method in the form of semi-structured interviews was used to collect data from 15 EFAL teachers who were purposefully selected on the basis that they used e-learning to supplement their face-to-face teaching. I found that the integration of e-learning to the traditional physical contact style was essential in improving the teaching of EFAL. The majority of participants, however, highlighted that they still encountered challenges related to heavy workload and efforts to complete items listed in the work programme. Inadequate training of teachers on e-learning, a lack of resources and poor connectivity also acted as barriers to the full implementation of blended learning, which considerably improves learners’ performance.

Experiences of workplace environment of neophyte registered nurses in selected hospitals of Limpopo province, South Africa

Experiences of workplace environment of neophyte registered nurses in selected hospitals of Limpopo province, South Africa

Setting up classroom libraries in rural areas: The case of Mogodumo circuit in Limpopo

There is an urgent need to establish classroom libraries, especially in rural areas where learners hardly come across any reading material. The purpose of this study was to investigate how teachers set up classroom libraries in rural areas of the Mogodumo circuit in the Limpopo province of South Africa. The study was qualitative in nature. Fifteen English First Additional Language (EFAL) teachers from 12 schools participated in the study. Observation and semi-structured interviews were employed to collect data. It was found that while all participants recognised the importance of reading for their learners, only 7 of the 15 teachers managed to establish classroom libraries. The study revealed that teacher self-efficacy (TSE) played a crucial role in setting up classroom libraries, given the serious challenges of funding, resources and support experienced by rural schoolteachers. Teachers with high TSE managed to establish classroom libraries by using their own time, money, and resources, while those with low TSE failed to establish classroom libraries. We recommend that school managers should make time and resources available to rural schoolteachers to enable them to establish classroom libraries.

Setting up classroom libraries in rural areas: The case of Mogodumo circuit in Limpopo

There is an urgent need to establish classroom libraries, especially in rural areas where learners hardly come across any reading material. The purpose of this study was to investigate how teachers set up classroom libraries in rural areas of the Mogodumo circuit in the Limpopo province of South Africa. The study was qualitative in nature. Fifteen English First Additional Language (EFAL) teachers from 12 schools participated in the study. Observation and semi-structured interviews were employed to collect data. It was found that while all participants recognised the importance of reading for their learners, only 7 of the 15 teachers managed to establish classroom libraries. The study revealed that teacher self-efficacy (TSE) played a crucial role in setting up classroom libraries, given the serious challenges of funding, resources and support experienced by rural schoolteachers. Teachers with high TSE managed to establish classroom libraries by using their own time, money, and resources, while those with low TSE failed to establish classroom libraries. We recommend that school managers should make time and resources available to rural schoolteachers to enable them to establish classroom libraries.