The objectives of this study were to understand dairy farmers' awareness and perceived barriers to the implementation of best management practices (BMP) for the transportation of lactating culled cows being moved to auction or abattoir. An invitation to participate was emailed to all dairy farms in Ontario, Canada. Responses to this invitation were used to recruit additional participants through criterion purposive snowball sampling. In total, 28 dairy producers participated in one of 5 semi-structured focus groups. All focus groups were audio recorded, transcribed verbatim, and analyzed with applied thematic analysis. Four themes were identified including 1) transfer of responsibility, 2) interpersonal relationships, 3) juggling priorities, and 4) complexities of long-term planning. While individual participant knowledge varied, participants described misconceptions that other producers held surrounding transport duration and distances, or the number of possible sales points for lactating culled cows. Participants did not agree whether the producer's responsibility ended once the cow left their property or if shared responsibility remained among all stakeholders from the farm to the final destination. Participants discussed the importance of existing trusted relationships with local cattle transporters and veterinarians to ensure information on best practices are shared. Participants also discussed how business pressures (e.g., production demands, space limitations) often challenge their ability to dry off lactating cows before transport; however, participants mentioned the degree of milk production may influence the destination of their animal (e.g., direct to slaughter for animals with high milk production). Some participants described a dynamic balance between business pressures and the potential for declining welfare of the animals under consideration to be culled during lactation. Participants posited that producers who did not prioritize proactive herd management and producers nearing retirement had limited long-term planning for culling individual cows, which might lead to transport of vulnerable or unfit animals. Finally, the lack of access to transportation direct to local slaughter was identified as an important barrier to adherence with the BMP for cull cows. In summary, many participants did not have an accurate understanding of what happens to cull cows after departing the farm and were unsure if they maintained a level of responsibility for an animal after being transported from their farm. Given the priority placed by participants on trusted interpersonal relationships, greater availability of peer-to-peer training of farmers and professional accreditation of animal transporters in addition to structural changes to increase local slaughter capacity may increase implementation of BMP and enhance lactating cull cow welfare.