Different forms of boron (B) were studied under long-term effect of chemical fertilizers and amendments in an acidic Alfisol of northwestern Himalayas. Residual B was dominant, comprising 71.5% of total B. Different B fractions correlated among themselves, showing interdependency of pools of B. On an average, all pools except for organically bound B exhibited depletion. Application of recommended doses of nitrogen (N), phosphorus (P), and potassium (K) with farmyard manure (FYM) in general showed less depletion in comparison to other treatments with the exception of liming treatment in oxide-bound B. Soil reaction (pH) had a significant positive relationship with most fractions. Except for residual and total B, significant positive relationships with other fractions of B with cation exchange capacity (CEC) were observed. Available B had a significant positive relationship with nonspecifically and specifically adsorbed B, indicating their importance in replenishing available B in soil. Imbalanced use of fertilizers had the most deleterious effects on soil properties.