Lime Peel Research Articles

Optimization strategies for bioethanol production from sweet lime peel waste: a study on simultaneous saccharification and fermentation with co-cultures

In the present study, sweet lime peel (SLP) is tested for the production of bioethanol. The acid-catalysed steam-pretreated SLP was studied for simultaneous saccharification and fermentation (SSF). Cellulase, pectinase and beta-glucosidase enzymes were used for the saccharification of pretreated SLP. SSF studies were performed using these enzymes and a co-culture of Saccharomyces cerevisiae, Zymomonas mobilis and Escherichia coli. The process variables such as peel loading (5–15%w/v), temperature (25–50 °C), pH (4–6) and fermentation time (24–72 h) were optimized for bioethanol using central composite design, and the effect of these variables on ethanol concentration was analysed. Ethanol concentration was maximum with a peel loading of 10% (w/v) fermented using the co-culture of S. cerevisiae, Z. mobilis and E. coli at a temperature of 37.5 °C and initial pH 5 for 48 h; 16.5 g/L of ethanol was produced at the optimum conditions with a fermentation yield of 89.6%.

Pembuatan Sabun Cuci Piring Cair Ramah Lingkungan BeEkstrak Kulit Jeruk Nipis (Citrus Aurantiifolia)

ish washing soap is a cleaner made by a chemical reaction between potassium or sodium and fatty acids from vegetable oils or animalfats. Making liquid dishwashing soap from lime peel extract has been carried out using the extraction method. This research aims todetermine the effect of lime peel extract and Methyl Ester Sulfonate (MES) on the characteristics of the liquid dishwashing soap produced,including several tests, namely pH, density, viscosity, bacteria based on SNI 06-2075-1996. Soap making is done by varying the volume oflime peel extract 3, 6, 9, 12, 15 ml and the MES mass 5, 10, 15, 20, 25, 30 grams, as well as adding other ingredients such as NaCl 8 grams,distilled water 300ml, aromatics 2 ml, dye 1 gram. The research results showed that a lime peel extract volume of 6 ml and a MES massof 15 grams produced the best soap with a pH of 6.04, density of 1.099 gr/ml, viscosity of 19.8 cP, bacteria 4 colonies/gr.

Herb-Fortified Arabica Cascara Infusion as a Functional Beverage

Cascara is a product derived from the drying of Arabica coffee pulp. It has potential as a functional beverage with health benefits. This study aims to produce a functional beverage from cascara and analyze its organoleptic properties, phytochemical content, antioxidant activity, total polyphenols, and caffeine content of cascara infusion fortified with medicinal plants. The methods utilized in this study included drying, grinding, and sieving, as well as the formulation of cascara with lemongrass (Cymbopogon citratus) and kaffir lime peel (Citrus hystrix) fortificants. The formulations included V1 (C : S = 75% : 25%); V2 (C : J = 75% : 25%); V3 (C : S : J = 50% : 25% : 25%); and V4 (C : S : J = 75% : 12,5% : 12,5%). The analysis included organoleptic evaluation, cascara brewing temperature and time optimization, gravimetric method moisture content evaluation, pH measurement, phytochemical content evaluation, DPPH method antioxidant activity assay, Folin-Ciocalteu method total polyphenol content measurement, and UV-Vis spectrophotometry caffeine content evaluation. The hedonic test results indicated that the most preferred sample was V1, with a score of 3.90 out of 5.00. Moisture content analysis showed that cascara contains 13.36 ± 0.0007% moisture, lemongrass stalks 11.10 ± 0.0043%, and kaffir lime peel 7.63 ± 0.0004%. The pH of all variants had met the standard with a range of 4.18 ± 0.05 - 4.24 ± 0.08. The phytochemical analysis indicated that the control variable of cascara infusion (C) and V2 contained alkaloids, phenolics, and tannins. Meanwhile, V1 contained alkaloids, phenolics, tannins, and terpenoids. All cascara infusion variants contains antioxidant activity ranged between 78,25 ± 0,25 - 79,50 ± 0,63%; total polyphenol 494,25 – 1.500 mg GAE/100g; and caffeine content 392,53 – 409,53 mg/100g. In conclusion, the study found that cascara infusion V1 was the most preferred by taste testers, and all the infusions were rich in antioxidants and other beneficial compounds.

Anaerobic Digestion of Lime Waste in Batch Assay Digestion for Potential Biogas Recovery

The term" lime" includes a wide variety of materials produced by calcining or burning limestone or dolostone. Lime and products containing lime have been widely employed in landfill covers (caps), wastes, and liners. The accumulation of lime wastes, an environmental hazard on dumpsites, causes economic and environmental problems. Nonetheless, there is a potential to recover biogas from lime wastes due to their high concentration in soluble sugars. The liming of waste is supposed to stop biodegradation and reduce the impacts of landfilling activity. D-Limonene, a chemical compound present in lime peels, is, however, known to negatively affect the anaerobic digestion and conversion of lime waste to biogas. This study is aimed at reducing the effect of DLimonene on biogas production from lime wastes. The study assessed the impact of lime waste pre-treatment and co-digestion with poultry litter on improving biogas recovery. This study further describes the adoption of a biodigestion system for the degradation of organic matter and biogas production. In co-digestion, an equal mass of lime waste was mixed with that of poultry litter. The pre-treatment of lime waste was done using a solid-liquid extraction process to filter out essential oil, containing D-Limonene, from lime wastes. Different samples from untreated, pre-treated, and mixed wastes were digested in laboratory under mesophilic conditions. The results indicate that the pre-treatment process alone increased biogas yield by about 40% while the co-digestion of untreated lime waste with poultry litter did not affect improving biogas yield. The use of these pre-treated samples in co-digestion with other nutrients filled and high energy yielding biomass would provide a better nutrient balanced and microbial diversity. Economic estimation of the biogas production process from lime waste that was carried out indicated lesser cost than the current market price of cooking gas in Nigeria. The findings show that there is economically viable recovery option of biogas from lime waste, which would boost the drive of government towards an alternative source of energy.

TOXICITY TEST OF LIME PEEL EXTRACT (<i>Citrus aurantifolia</i>) USING THE BRINE SHRIMP LETHALITY TEST (BSLT) METHOD FOR DETECTION OF ANTI CANCER ACTIVITY

Cancer is one of the major problems affecting the people of Indonesia and the world. Lack of public awareness for early detection causes a delay in diagnosis. One of the medicinal plants that are commonly found in Indonesia is lime (C. aurantifolia). In lime peel extract, there is essential oil composed of D-Limonene (38.94%), β-pinen (26.66%), α-terpineol (8.29%), and terpinen-4-ol (4.32%). These compounds are known to have strong effects as anti-cancer. This study used a completely randomized design (CRD) with 5 treatments and 5 repetitions, namely 0, 125, 150, 250, and 1000 ppm. The research methods used were phytochemical test, FTIR (Fourier-Transform Infrared Spectroscopy) analysis, and BSLT test. The probit value was analyzed using SPSS to obtain the LC50 value. Lime peel extract (C. aurantifolia) has an LC50 value of 331.533 ppm including the toxic category and has potential as an anti-cancer agent. Based on phytochemical and FTIR tests, lime peel (C. aurantifolia) contains alkaloid, flavonoid, and tannin compounds which are known to have antioxidant properties and can disrupt the balance of Reactive Oxygen Species (ROS) so as to trigger apoptosis of cancer cells.

Extraction of pectin from powdered citrus peels using various acids: An analysis contrasting orange with lime

Pectin, a vital hydrocolloid, is widely utilized in food, medicine, and cosmetics for its gelling, thickening, and stabilizing properties. Although citrus peels are used for pectin production, there is a need to enhance extraction efficiency and identify the optimal methods for commercial use. This study aimed to optimize pectin extraction from orange peel powder (OPP) and lime peel powder (LPP) using different acids (0.05M HCl, H₂SO₄, HNO₃, and citric acid) under controlled conditions (80°C, pH 2.0) for 60 and 90 min. The highest pectin yield was achieved at 90 min, with sulfuric acid yielding 21.63% from OPP and citric acid producing 32.53% from LPP, confirmed by principal component analysis (PCA). The extracted pectin was analyzed for several key characteristics, including moisture content, ash content, equivalent weight, methoxyl content, anhydrouronic acid (AUA) content, and solubility in hot and cold water, and hot and cold alkali. Pectin from OPP exhibited higher moisture and ash levels than that from LPP. Specifically, OPP-derived pectin demonstrated 61.65% esterification, 7.29% methoxyl content, 67.19% AUA content, and an equivalent weight of 683.41 mg/mL. In contrast, pectin extracted from LPP showed 66.39% esterification, 7.73% methoxyl content, 66.09% AUA content, and a higher equivalent weight of 792.57 mg/mL. Both OPP and LPP yielded high-methoxyl pectin suitable for gelling applications. PCA revealed that pectin extracted from LPP had superior values for equivalent weight, methoxyl content, and esterification degree compared to OPP. Interestingly, AUA content negatively correlated with these properties, while other parameters were positively correlated. These findings indicate that using citric acid to extract pectin from lime peels could outperform current commercial methods, yielding high-methoxyl pectin with excellent gelling properties. This makes it especially valuable for industrial applications in the food, pharmaceutical, and cosmetic sectors.

Organic farming innovations: Cultivation of wheatgrass microgreens in soil enriched with fruit peels and antimicrobial assessment

ObjectiveThis study explores the viability of fruit peel-enriched soils as a sustainable growth medium, acting as a biofertilizer for wheatgrass microgreens. Additionally, the research seeks to evaluate the antimicrobial properties of fruit peels, typically considered agricultural waste, to determine their influence on plant growth parameters. MethodsFruit peels from pomegranate, orange, and sweet lime were collected and processed into powder, and diluted with water to create enriched soil. Wheatgrass microgreens were cultivated in conventional potting soil and soil enriched with fruit peel powder. Growth parameters, including germination rates, shoot and root length, and total yield of wheatgrass microgreens, were monitored over a 15-day growth cycle. Antimicrobial analysis was conducted on selected fruit peels, assessing their impact on Staphylococcus aureus and Escherichia coli compared to a control (Rifampicin). ResultsThe results, analyzed through ANOVA and Tukey (post hoc) tests, indicate significant differences among fruit peel-enriched soils. Pomegranate peel emerged as a particularly effective enhancer of wheatgrass microgreen growth. Furthermore, the antimicrobial analysis revealed that pomegranate and sweet lime peels exhibited superior properties, with a notable zone of inhibition effects on Staphylococcus aureus and Escherichia coli compared to the control (Rifampicin). ConclusionThis study demonstrates the potential of fruit peels as effective biofertilizers to promote wheatgrass microgreen growth in sustainable agricultural practices. The multifaceted benefits include enhanced crop development and the discovery of natural antimicrobial agents, particularly in pomegranate and sweet lime peels. These findings support the broader adoption of environmentally conscious approaches in agriculture, emphasizing the value of utilizing agricultural waste for plant growth promotion and the development of natural alternatives to synthetic antimicrobial agents.

Mineral concentration in citrus peel flours

Citrus fruits are known for their health benefits but managing processing waste is a challenge. Extracting bioactive compounds from these residues to create new products is promising, but mineral analysis is essential for food safety. The objective of this study was to analyze how the methodology for obtaining flour from sweet passion fruit peel, Tahiti lime, Murcott tangerine, and pearl pineapple influences the amounts of minerals Fe, Zn, Mn, Cu, Cr, and to determine the presence of these minerals in flours according to the defined limits. Maceration demonstrated a significant reduction in mineral content, such as iron, zinc, manganese, copper, and chromium, in the flours of lime and tangerine peels and passion fruit albedo. There was an increase in mineral levels after this process, such as zinc in passion fruit flavedo flour and manganese in tangerine and pineapple peels. Fe, Zn, Mn, Cu, Cr were respectively determined in 100g-1 in flours, from lime peel: 3,696mg; 1,273mg; 22,834mg, 0.422mg; 1104.945 μg; tangerine peel: 1,322mg; 2,560mg; 13,571mg; 0.236mg; passion fruit albedo: 8,596mg; 6,480mg; 76,003mg; 1,031mg; passion fruit flavedo 8,609mg; 3,363mg; 86,167mg; 2,317mg; in pineapple skin: 12,181mg; 0.704mg; 71,018mg; *mg; 2224.215 μg. Cr was not determined in the flours: albedo and flavedo from passion fruit, and tangerine and Cu from pineapple peel. Flours are sources of minerals but must be consumed with caution due to high quantities that can exceed the maximum tolerable intake limits.

Sweet lime peel biomass derived nano-cellulose for dye removal: Kinetics, thermodynamics, optimization and toxicity studies

Sweet lime peel biomass derived nano-cellulose for dye removal: Kinetics, thermodynamics, optimization and toxicity studies

Antibacterial Activities and Chemical Composition of Essential Oils from Sweet Orange (Citrus sinensis), Lemon Grass (Cymbopogon citratus), and Lime (Citrus aurantifolia) Peels

Antibacterial Activities and Chemical Composition of Essential Oils from Sweet Orange (Citrus sinensis), Lemon Grass (Cymbopogon citratus), and Lime (Citrus aurantifolia) Peels

Novel neuropharmacological activity of citrus lime (Citrus aurantifolia): A standardized lime peel supplement enhances non-rapid eye movement sleep by activating the GABA type A receptor

Polyphenols have been well-established to exert sedative-hypnotic effects in psychopharmacology. Lime (Citrus aurantifolia) peel is rich in biologically active polyphenols; however, the effects of lime peel extract on sleep have not yet been demonstrated. A comparison was conducted in mice, between the sleep-promoting effects of a standardized lime peel supplement (SLPS) and a well-known hypnotic drug, zolpidem, and its hypnotic mechanism was investigated using in vivo and in vitro assays. The effects of SLPS on sleep were assessed using a pentobarbital-induced sleep test and sleep architecture analysis based on recording electroencephalograms and electromyograms. Additionally, a GABAA receptor binding assay, electrophysiological measurements, and in vivo animal models were used to elucidate the hypnotic mechanism. SLPS (200 and 400 mg/kg) was found to significantly decrease sleep latency and increase the amount of non-rapid eye movement sleep without altering delta activity. The hypnotic effects of SLPS were attributed to its flavonoid-rich ethyl acetate fraction. SLPS had a binding affinity to the GABA-binding site of the GABAA receptor and directly activated the GABAA receptors. The hypnotic effects and GABAA receptor activity of SLPS were completely blocked by bicuculline, a competitive antagonist of the GABAA receptor, in both in vitro and in vivo assays. To the best of our knowledge, this study is the first to demonstrate the hypnotic effects of SLPS, which acts via the GABA-binding site of the GABAA receptor. Our results suggest that lime peel, a by-product abundantly generated during juice processing, can potentially be used as a novel sedative-hypnotic.

Investigating Ethanolic Extract from Acehnese Lime (Citrus aurantifolia) Peel as Potential Anti-Hypercholesterolemia Agent

Lime peels are rich in flavonoids, alkaloids, phenols, saponins, and tannins, exhibiting antibacterial, antioxidant, anti-inflammatory, anti-hypertensive, and anti-hypercholesterolemia properties. However, the specific active constituents of Acehnese lime peels and their impact on anti-hypercholesterolemia effects remain undisclosed. This present investigation aims to identify the active compounds in the ethanolic extract of locally obtained Acehnese lime (Citrus aurantifolia (Christm.) Swingle) peels and assess their potential as inhibitors of Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) and 3-hydroxy-3-methylglutaryl-coenzyme-A (HMG Co-A) reductase using in-silico approach. The composition of the ethanolic extract Acehnese lime peel was determined through phytochemical analysis, 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, gas chromatography-mass spectrometry (GC-MS) analysis, and predictions of biological activity, while the biological activities of their compounds were evaluated through molecular docking. Phytochemicals revealed the presence of phenolics, flavonoids, tannins, saponins, and terpenoids in the ethanolic extract of local Aceh lime peel. The total phenolic and flavonoid contents were 29.992 ± 0.274 mg gallic acid equivalents (GAE)/g extract and 5.983 ± 0.017 mg quercetin equivalents (QE)/g extract, respectively. The anti-oxidant activity was notably strong with an IC50 value of 49.51 ppm. GC-MS analysis identified 6-methoxychroman-2-one (27.64%) as the primary component in ethanolic extract Acehnese lime peel, along with neric acid (C13H22O2) known as a regulator of lipid metabolism (Pa: 0.941). In-silicoinvestigations indicated that pterin-6-carboxylic acid (-7.8 kcal/mol) exhibited a higher binding free energy for the PCSK9 receptor compared to simvastatin (-7.6 kcal/mol), whereas the active compound (R)-9-(2,3-dihydroxy-3-methylbutoxy)-4- exhibited the highest binding capacity for HMG Co-A reductase (-6.9 kcal/mol) compared to other compounds. These findings suggest that the ethanolic extract of Acehnese lime peels could serve as an effective inhibitor of PCSK9 and HMG Co-A reductase, highlighting its potential as a novel anti-hypercholesterolemia agent. Doi: 10.28991/HEF-2024-05-03-04 Full Text: PDF

Antibacterial Activity for Synthesized Coumarin Derivatives and a Coumarin Component of Lime Peel (Citrus aurantifolia).

In this study, we investigated the antibacterial activity of the coumarin component isolated from lime peel and coumarin derivatives synthesized using various techniques against eight types of food-poisoning bacteria. The minimum inhibitory concentration (MIC) for the 3b [5,7-dihydroxy-4-trifluoromethylcoumarin] derivative was measured as 1.5 mM in Bacillus cereus, Micrococcus luteus, Listeria monocytogenes, and Staphylococcus aureus subsp. aureus; that for the 3c [7-hydroxy-4-trifluoromethylcoumarin] derivative was 1.7 mM in Enterococcus facium; and that for the 3n [dicoumarol] derivative was 1.2 mM in L. monocytogenes. These results confirmed that coumarin derivatives with CF3 and OH substituents had enhanced antibacterial activity.

Optimization and characterization of essential oil-loaded nanoemulsions for enhanced antiviral activity against tobacco mosaic virus (TMV) in tobacco plants

Tobacco mosaic virus (TMV) is a significant viral pathogen that infects various economically important crops, including tobacco, tomatoes, and cucumbers. The current study aimed to develop and optimize essential oil-loaded nanoemulsions using Response Surface Methodology (RSM) to enhance antiviral activity against TMV in Nicotiana tabacum plants. Essential oils from tea tree (Melaleuca alternifolia) and lime peel (Citrus latifolia) were chemically analyzed using gas chromatography-mass spectrometry (GC-MS). Terpinene-4-ol (70.92 %) and trans-isolimonene (33.82 %) were identified as the major components in tea tree and lime peel oils, respectively. RSM was used to optimize the nanoemulsion formulations based on oil concentration, hydrophilic-lipophilic balance (HLB), and stirring speed. Optimal tea tree oil (TTEO) and lime peel oil (LPEO) nanoemulsions were developed and characterized using Fourier-transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and particle size analysis. The antiviral efficacy of the optimized formulations was tested against TMV using RT-qPCR analysis and physiological measurements. TTEO nanoemulsions significantly inhibited TMV expression by 89.78 %, while LPEO nanoemulsions achieved a 42.22 % reduction after six days. Additionally, these essential oils (EOs) enhanced plant defense mechanisms by modulating the activities of phenylalanine ammonia-lyase (PAL) and peroxidase (POD), along with maintaining chlorophyll levels. This study demonstrates that TTEO and LPEO nanoemulsions offer promising eco-friendly antiviral agents for TMV management in tobacco plants. The research underscores the potential of essential oil-based formulations in mitigating viral infections in crops, providing a sustainable alternative to synthetic chemicals.

Uji Bioaktivitas Fraksi Etanol Ekstrak Kulit Buah Jeruk Purut Citrus hystrix Sebagai Anti Bakteri Staphilococus aureus dan Escherecia coli

Plants have several ways to protect themselves from bacteria, one of which is by producing compounds that are toxic or repellent to bacteria. Compounds produced by plants are known as secondary metabolite compounds which originate from secondary metabolic processes. Several secondary metabolite compounds such as flavonoids, alkaloids, saponins, tannins, steroids and terpenoids are currently widely used as antibacterials. One of the plants that has the potential to be developed as a herbal medicine is kaffir lime leaves. Citrus hystrix is ​​the part of the plant most often used by the public. Both for cooking and traditional medicine purposes. In this research, it is hoped that lime peel extract can act as an antibacterial for Escherichia coli and staphylococcus aereus, so that it can be used as a herbal plant for diseases caused by these two bacteria. In the research, the concentrations used for each test bacteria were 50%; 25%; 12.5%. in observing S. aureus bacteria 1x24 hours with a concentration of 50%, the size of the inhibitory zone was 16 mm, the 25% concentration was 11 mm and the 12.5% ​​concentration was 9 mm. Meanwhile, E. Coli bacteria did not show any reaction to the heruk peel extract.

Comparison of Different Extraction and Purification Methods of Lime Peel Pectin and Evaluation of Physiochemical, Rheological, and Textural Properties

Background:: Pectin production has recently attracted considerable research interest due to its various applications arising from its unique characteristics. Lime peel, as a by-product of juice factories, has a promising potential for pectin extraction. Methods:: Lime peel pectin samples were extracted by three extraction processes: a) 90°C of heating for 120 min, b) 90°C of heating for 90 min and then 32 min of sonication, and c) 80°C of heating for 60 min and then 22 min of sonication. Then, they were purified either by ethanol or sodium caseinate (SC) and characterized. Results:: SC purification did not enhance the pectin yield; rather, it diminished the level of nonpectin components and resulted in purer pectin (i.e., lower sugar content). The samples purified with SC showed much lower viscosity in solution and storage modulus than the samples purified with ethanol, which could be attributed to their lower sugar content and higher pH (~ 4.6 vs. 2). However, pectin samples purified with SC had a relatively high sugar gel consistency, maybe due to protein retention in their structure as well as the preferential hydration effect of added sucrose. Conclusion:: The compact and extended conformations of the pectin sample, which was extracted by “(c) extraction method” and purified with ethanol, resulted in its high viscosity and storage modulus in solution and the highest gel consistency (16.91 Kg.s) compared to the other samples. Regardless of the conditions of extraction procedures, the purification method had a considerable impact on the pectin samples’ characteristics and potential applications.

Repellent Activity Testing of an Essential Oil Gel Preparation of Cinnamon (Cinnamomun Burmanii) Bark) And Kaffir Lime Peel (Citrus Hytrix) Against Aedes Aegypti Mosquitoes

Dengue fever is still a disease that dominates, especially in the Papua region. To overcome this, it is necessary to modify preparations based on medicinal plants as mosquito repellents, one of which is gel preparations. This study aims to determine the repellent activity test of essential oil gel preparations of Cinnamon Bark (Cinnamomum burmannii) and Kaffir Lime Peel (Citrus Hystrix) against the Aedes aegypti mosquito. The method used in this research uses experimental methods. This research was conducted in April - November 2022. This research used two formulations, namely cinnamon bark and kaffir lime peel (FI) formulations which were compared with formulation preparations in the form of a base without active substances (FII). The tests carried out were physical quality tests and repellent tests on the Aedes aegypti mosquito. The research results show that based on physical quality tests, it is known that FI has a distinctive odor, slightly yellowish white color and a thick shape. Meanwhile, FII has the distinctive smell of patchouli oil, milky white color and thick shape. In the homogeneity test on FI and FII it was found to be homogeneous and fulfilled the requirements. In the adhesion and spreadability tests on FI and FII it was found that they met the requirements. In the pH test, the pH of FI and FII has a pH value of 7 and meets the requirements. In the repellent test using arm-in-cage with 3 replications using 20 Aedes aegypti mosquitoes. The formulas tested were FI and FII and observations made were by recording the number of mosquitoes that landed after using the repellent for 15, 30, 60, 120, 240 and 360 minutes. The research results showed that in the 15th minute the protection power was 88.3%, in the 30th minute the protection power was 88.3%, in the 60th minute the protection power was 72.3%, in the 120th minute the protection power was 67, 1%, in the 240th minute the protection power was 51.5%, and in the 360th minute the protection power was 47.7%. Based on the research results, it can be concluded that the essential oil formulation of cinnamon bark and Kaffir lime peel is efficacious as a repellent.

Formulation of Handwash Essential Oil and Hydrosol of Lime Peel (Citrus aurantifolia) as Antibacterials

Formulation of Handwash Essential Oil and Hydrosol of Lime Peel (Citrus aurantifolia) as Antibacterials

Sustainable extraction of proteins from lime peels using ultrasound, deep eutectic solvents, and pressurized liquids, as a source of bioactive peptides

The aim of this work was to develop, for the first time, sustainable strategies, based on the use of Ultrasound-Assisted Extraction, Natural Deep Eutectic Solvents, and Pressurized Liquid Extraction, to extract proteins from lime (Citrus x latifolia) peels and to evaluate their potential to release bioactive peptides. PLE showed the largest extraction of proteins (66–69%), which were hydrolysed using three different enzymes (Alcalase 2.4 L FG, Alcalase®PURE 2.4 L, and Thermolysin). The in vitro antioxidant and antihypertensive activities of released peptides were evaluated. Although all hydrolysates showed antioxidant and antihypertensive activity, the hydrolysate obtained with Thermolysin showed the most significant values. Since the Total Phenolic Content in all hydrolysates was low, peptides were likely the main contributors to these bioactivities. Hydrolysates were analyzed by UHPLC-QTOF-MS and a total of 98 different peptides were identified. Most of these peptides were rich in amino acids associated with antioxidant activity.

Immunomodulatory Effects of Ethanol Extract of Lime Peel on Phagocytosis Activity and Delayed-Type Hypersensitivity Response

The natural human environment is inhabited by various organisms, including those harmful to the body such as bacteria, viruses, fungi, and other pathogens. This condition weakens the immune system's response to pathogens, making the body more susceptible to diseases such as rheumatoid arthritis, cancer, and other inflammations. In some cases, this condition can be fatal, leading to death. This study aims to examine the immunomodulatory effects of Ethanol Extract of Lime Peel on phagocytosis activity and delayed-type hypersensitivity response in experimental animals. The research methods include sample collection and processing, plant identification, extract preparation, and immunomodulatory effect testing. Phytochemical screening results showed the presence of alkaloids, glycosides, flavonoids, tannins, and steroids in the extract of the lime peel. Simplicia and ethanol extract of lime peel characterization yielded results meet the standards of the Indonesian Herbal Pharmacopoeia 2nd edition. Immunomodulatory effect testing was conducted through phagocytosis activity and delayed-type hypersensitivity response assays. The results showed that the extract at doses of 25, 50, and 100 mg/kgBW had immunostimulatory effects in enhancing phagocytosis activity, while doses of 17.5 mg/kgBW, 35 mg/kgBW, and 70 mg/kgBW significantly increased delayed-type hypersensitivity response compared to the negative control. These findings indicate the potential of ethanol extract of Citrus aurantifolia as an immunomodulator that can enhance the body's immune system. This study provides an important contribution to the development of immunomodulatory therapy derived from natural ingredients such as lime peel for managing immunity-related diseases with lower side effects.