Lime Peel Extract Research Articles

Novel neuropharmacological activity of citrus lime (Citrus aurantifolia): A standardized lime peel supplement enhances non-rapid eye movement sleep by activating the GABA type A receptor

Polyphenols have been well-established to exert sedative-hypnotic effects in psychopharmacology. Lime (Citrus aurantifolia) peel is rich in biologically active polyphenols; however, the effects of lime peel extract on sleep have not yet been demonstrated. A comparison was conducted in mice, between the sleep-promoting effects of a standardized lime peel supplement (SLPS) and a well-known hypnotic drug, zolpidem, and its hypnotic mechanism was investigated using in vivo and in vitro assays. The effects of SLPS on sleep were assessed using a pentobarbital-induced sleep test and sleep architecture analysis based on recording electroencephalograms and electromyograms. Additionally, a GABAA receptor binding assay, electrophysiological measurements, and in vivo animal models were used to elucidate the hypnotic mechanism. SLPS (200 and 400 mg/kg) was found to significantly decrease sleep latency and increase the amount of non-rapid eye movement sleep without altering delta activity. The hypnotic effects of SLPS were attributed to its flavonoid-rich ethyl acetate fraction. SLPS had a binding affinity to the GABA-binding site of the GABAA receptor and directly activated the GABAA receptors. The hypnotic effects and GABAA receptor activity of SLPS were completely blocked by bicuculline, a competitive antagonist of the GABAA receptor, in both in vitro and in vivo assays. To the best of our knowledge, this study is the first to demonstrate the hypnotic effects of SLPS, which acts via the GABA-binding site of the GABAA receptor. Our results suggest that lime peel, a by-product abundantly generated during juice processing, can potentially be used as a novel sedative-hypnotic.

Uji Bioaktivitas Fraksi Etanol Ekstrak Kulit Buah Jeruk Purut Citrus hystrix Sebagai Anti Bakteri Staphilococus aureus dan Escherecia coli

Plants have several ways to protect themselves from bacteria, one of which is by producing compounds that are toxic or repellent to bacteria. Compounds produced by plants are known as secondary metabolite compounds which originate from secondary metabolic processes. Several secondary metabolite compounds such as flavonoids, alkaloids, saponins, tannins, steroids and terpenoids are currently widely used as antibacterials. One of the plants that has the potential to be developed as a herbal medicine is kaffir lime leaves. Citrus hystrix is ​​the part of the plant most often used by the public. Both for cooking and traditional medicine purposes. In this research, it is hoped that lime peel extract can act as an antibacterial for Escherichia coli and staphylococcus aereus, so that it can be used as a herbal plant for diseases caused by these two bacteria. In the research, the concentrations used for each test bacteria were 50%; 25%; 12.5%. in observing S. aureus bacteria 1x24 hours with a concentration of 50%, the size of the inhibitory zone was 16 mm, the 25% concentration was 11 mm and the 12.5% ​​concentration was 9 mm. Meanwhile, E. Coli bacteria did not show any reaction to the heruk peel extract.

FORMULASI DAN EVALUASI SEDIAAN SERUM WAJAH EKSTRAK ETANOL KOMBINASI DARI EKSTRAK KULIT JERUK NIPIS (Citrus aurantiifolia) DAN EKSTRAK KULIT BUAH NAGA MERAH (Hylocereus polyrhizus)

Background: The skin of the key orange (Citrus aurantiifolia) contains kuersetin, miristin, routinely, tangerine, naringin and hesperine, while the skin of the red dragon fruit (Hylocereus polyrhizus) contains betaalanin, anthocyanin, vitamin a, vitamin a, vitamin e, turnioid, flavonoid, tiamin, niacin, piridoine, kobalamin, carotene, and fitoalbumin. The research was intended to formulate it in a availability of facial serum and then evaluate its quality formula for a better facial serum from 16-4954-1998. Methods : This method of research is experimental in the laboratory by making 4 formulas consisting of F1 as serum bases, F2 ratio EKJN and EKBNM (1:1), F3 ratio EKJN and EKBNM (1:2) ,F4 ratio EKJN and EKBNM (2:1).Results : The results of all the formulas for existing existing face-serum EKJN ethanol and EKBNM viscers show a clear, clear, yellowish color, a gel-based, characteristic and homogenous odor. pH and viscosity scores on each condensed formula. No irritation test on the formula showed irritation to the skin.Conclusion : Based on this, it can be concluded that the combined ethanol extract of lime peel extract and red dragon fruit peel extract can be formulated into a facial serum cosmetic preparation and meets the quality requirements according to SNI.

Formulation and SPF Value Evaluation of Sunscreen Spray Gel Containing Lime Peel Extract (Citrus aurantifolia)

Background: High exposure to sunlight has adverse effects on the skin. Lime peel contains more than 60% flavonoids, presenting the potential to function as a sunscreen due to the presence of conjugated aromatic benzene groups, capable of absorbing UV-A or UV-B rays from the sun. To prevent skin damage, lime peel extract is formulated into a spray gel, as it has the ability to dry rapidly, enhancing overall comfort for consumers during application. Objective: To determine the influence of variation concentration of lime peel extract in the sunscreen spray gel on its physical properties and in vitro SPF value. Methods: Lime peel crude extract was obtained using 70% ethanol and formulated into a sunscreen spray gel at concentrations of 5%, 10%, and 15%. The spray gel formulation was evaluated for its physical quality and SPF value. Results: The variation in extract concentration has a statistically significant effect on the physical properties of the preparation and SPF values (P<0.05). The physical stability conditions in each formula (F1, F2, and F3) meet the requirements of the spray gel preparation in terms of pH, viscosity, spreading test, drying time test, and adhesion test. The spray gel preparations F1 (5%), F2 (10%), F3 (15%) each have SPF values of 20, 25, and 35 respectively. Conclusion: The spray gel formulations in F1 (5%), F2 (10%), and F3 (15%) are physically stable and have moderate to high SPF values, with F3 (15%) having the highest SPF value of 35.

The preliminary study on the antifungal effect of Kaffir lime (Citrus hystrix DC) peel extract against Malassezia furfur

Pityriasis versicolor is one of the most common fungal infections on the skin caused by Malassezia furfur. Kaffir lime fruit (Citrus hystrix DC), especially its peels’ contents in secondary metabolite, may play a healing role against such infections. Therefore, the aim of this study was to determine the antifungal potential of Kaffir lime peel extract against Malassezia furfur. Ethanol extracts of the peels of Kaffir lime were tested for its antifungal properties against Malassezia furfur at different tested concentrations, namely 25%, 50%, 75%, and 100%. Every treatment was performed with 4 replications. Inhibition zone formed surrounding the paper disc measured after 24 hours of incubation using the caliper method. Data were subjected to the Kruskal-Wallis test and the Mann-Whitney Post hoc test. The study revealed that the kaffir lime fruit extract gave a significant effect on the inhibition zone (p< 0.05). The results showed that the higher inhibition zone was found at control group and it was different significantly from other treatments. However, among the tested concentrations, the best treatment was detected at a concentration of 100%, which was significantly different from other concentrations. Kaffir lime peel has an antifungal effect against Malassezia furfur and the best tested concentration was 100%.

Combination of Coffee Grounds (Coffea) and Lime Peel Extract (Citrus Aurantifolia) in Making Body Scrub

Coffee grounds and lime peel extract can be used as raw materials for making environmentally friendly Body Scrub. This research aims to determine the effect of coffee grounds and lime peel extract and the effect of their use on the finished Body Scrub. A combination of coffee grounds and lime peel extract is used in variations of 15 g, 20 g, 25 g, 30 g, 35 g, and 40 g, for coffee grounds, while for lime peel extract the variations are 2 g, 2.5 g, 3 g, 3.5 g, and 4 g. Tests carried out include organoleptic tests, pH tests, homogeneity tests, microbial contamination tests, irritation tests, and existing humidity tests on volunteers. The research results that the body scrub preparation met the evaluation of the physical preparation of the Body Scrub preparation, namely coffee aroma, brown color and semi-solid texture, pH ranged from 5.2-5.9, homogeneous, didn’t irritate the skin, and could increase moisture in the skin.

Potential of Solid Gel Formulation of Kaffir Lime Peel Extract Against Postharvest Pest Sitophilus Zeamais on Stored Rice

Maize weevil, Sitophilus zeamais (Motschulsky) (Coleoptera: Curculionidae) is a primary pest on maize, rice, wheat, sorghum, and other cereal grains. The purpose of this study was to determine the effectiveness of using kaffir lime peel extract in solid gel formulation as a plant-based insecticide. This study used five treatments, untreated (control), 0.5%, 1%, 1.5%, and 2%. The results of this study are the treatments tested had effects on adult mortality and repellency. The 2% concentration showed the highest adult mortality and repellency. The major volatile organic compounds contained in kaffir lime peel were α-fernesene, α-sinensal, D-citronellol, and citronellal.

Sustainable ZnO nanoparticles using sweet lime peel extract: Eco-friendly activator in rubber for tire applications

Sustainable ZnO nanoparticles using sweet lime peel extract: Eco-friendly activator in rubber for tire applications

Gutta-percha dissolving ability of Citrus limetta, essential oils and a customized emulsion: An in vitro study

Aim: This study aims to evaluate the GP dissolving property of ethanolic extract of sweet lime peel (Citrus limetta), peppermint oil, cedarwood oil, and an emulsion (equal volumes of ethanolic extract of sweet lime peel, peppermint, and cedarwood oils) compared to xylene and chloroform (CHCl3). Methods: Equal weight of GP were divided into 7 groups and immersed for 5 min and 10 min in the test and control groups. Distilled water was used as a negative control. Absolute weight loss (WL) was calculated. Results: Data were analyzed using Kruskal–Wallis test followed by Dunn’s pairwise comparison with a significance value of P ≤ 0.05. The median WL of GP in the solvents shows a significant difference at 5 min (P = 0.001) and 10 min (P = 0.000). After 5 min, the order of WL was highest in sweet lime peel extract, CHCl3, and emulsion followed by xylene and peppermint oil. After 10 min, the highest WL was seen in emulsion, CHCl3 followed by sweet lime peel and peppermint oil. Cedarwood oil had no GP solvent effect at both time intervals. Conclusion: Sweet lime peel extract, emulsion, and peppermint oil showed a significant GP dissolving effect at 5 and 10 min and were comparable to CHCl3. Cedarwood oil showed no GP dissolving effect.

THE EFFECTIVENESS OF ORANGE PEEL EXTRACT TOOTHPASTE IN LIGHTENING TEETH AND PREVENTING BACTERIAL INFECTIONS LEADS TO HALITOSIS

Introduction. Halitosis is a dental and oral illness that is common in society. Steptococcus salivarius is the bacteria that causes halitosis. The purpose of this study was to determine whether lime peel extract toothpaste possesses any bioactive antibacterial properties against the strain of bacteria Streptococcus salivarius and can brighten teeth. Materials and Methods. The research method used was True Experimental Design. This research design used Post Test Only Control Design. Where there are three groups, namely the treatment group, positive control group, and negative control group. The data obtained will be tested statistically using the one-way ANOVA analysis with a confidence level of 95% and followed by the Least Significance test. Dental lightening research is carried out on teeth that have been extracted. Brush the teeth with lime peel paste for 7 days and do it 2 times a day. Tooth color was recorded before treatment and after treatment with shade guide Chromascop Ivoclar Vivedent. Result and Discussion. The results of this study showed that toothpaste with lime peel extract was able to inhibit the growth of Streptococcus salivarius bacteria. The respective concentrations of lime extract in toothpaste F0 to F4 are 0%, 2.5%, 5%, 7.5%, and 10%. K+ about using toothpaste on the market, with an average inhibition zone of F1 (8.7), F2 (9.86), F3 (11.58), F4 (13.4), K+ (19.65), and K- (0), so it can be stated that the more the extract content, the higher the inhibitory power for the growth of Streptococcus salivarius. Besides that, lime peel toothpaste can brighten teeth several levels Conclusion: Lime peel extract toothpaste is able to inhibit the growth of Streptococcus salivarius bacteria and is able to brighten teeth several levels.

The number of lymphocytes in rabbit gingival wounds covered with a periodontal pack with lime peel extract

Link or Video Abstract: https://youtu.be/oy1cDLwM0M8Introduction: Periodontal packs have frequently received extra anti-inflammatory and antibiotic medications, but they might trigger allergic reactions. As a result, alternative materials are required, such as herbal plants like lime peel, which can hasten wound healing without producing negative side effects. Citrus aurantifolia Swingle's peel extract, which has active ingredients with anti-inflammatory, antioxidant, antimicrobial, and antibacterial actions, can hasten the healing of wounds when added to periodontal packs. One marker in the healing process is reducing the production of lymphocytes. This study aims to determine the effect of lime (Citrus aurantifolia Swingle) peel extract on periodontal packs and the number of lymphocytes in rabbit gingival preparations. Methods: Thirty-two New Zealand white rabbits were employed as the sample; they were harmed on the mandibular gingiva using a punch biopsy with a diameter of 2 mm. The animals were split into eight groups on the third and fifth days, with the treatment group receiving a periodontal pack containing lime (Citrus aurantifolia Swingle) peel extract and the control group receiving a periodontal pack without it. In order to count the lymphocytes, histological preparations were examined using HE staining. Results: Statistical test results showed a significant difference in the number of lymphocytes between the control group and the treatment group on days 3 and 5 (ANOVA, p < 0.05). Conclusion: Lime (Citrus aurantifolia Swingle) peel extract can decrease the number of gingival lymphocytes in rabbit's periodontal tissue.

Uji Potensi Antibakteri Ekstrak Kulit Jeruk Nipis Citrus aurantifolia (Christm) Swingle Terhadap Vibrio sp.

Lime (Citrus aurantifolia) is one of the herbal plants that is rich in benefits, especially as a herbal medicines. Besides of lime fruit, lime peel also has antibacterial properties such as essential oils which contain flavonoids which can inhibit bacterial growth. This research utilizes lime peel which will be extracted as a test material. The purpose of this research is to determine the antibacterial potential of lime peel extract against Vibrio sp. at various concentrations. This research used the well-diffusion method using MHA as a test medium to determine whether lime peel extract can inhibit the growth of Vibrio sp. Various concentrations and penicillin as a positive control used in this research to determine which concentration has a greatest potential for inhibiting the growth of Vibrio sp. Result of this study used qualitative analysis by comparing the clear zone size produced on the medium. The obtained result shown that 20%, 40%, 60%, 80% and 100% concentration of lime peel can forming a clear zone which mean they can inhibit the growth of Vibrio sp. The best concentration used to inhibit Vibrio sp. is 100% concentration which produce 23.7 mm clear zone on MHA medium. Lime peel extract can be used to inhibit Vibrio sp. growth based on the research result.

The Potential of Lime Peel Extract Mouthwash as A Non-Pharmacological Preparation Inhibits Caries Bacteria

Dental caries is a disease of the hard tissue of the teeth that attacks the enamel, dentin and cementum which is characterized by the demineralization of the hard tissue of the teeth. Streptococcus mutans bacteria itself is one of the bacteria found and lives in the oral cavity. One of the infectious diseases caused by the process of removing minerals due to bacteria on the surface of the teeth is called caries. An alternative choice as an effort to solve the problem of inhibiting caries-causing bacteria is by using herbal mouthwash. One of them is lime peel. Lime peel has the potential to inhibit Streptococcus mutans bacteria as indicated by the formation of an inhibition zone, which is formed against Streptococcus mutans bacteria. Making a mouthwash preparation of lime peel extract (Citrus Aurantifolia) which is feasible and effective in inhibiting the growth of Streptococcus mutans bacteria. This research is a true experiment with post test only control group design. This design was chosen because it was grouped into treatment and control groups. The intervention was given in the form of lime peel extract mouthwash with concentrations of 15%, 25% and 50%, while the administration of the UNISSULA Faculty of Medicine Biomedical Laboratory Mouthwash Formula was the control group. Based on the average inhibition graph within 24 hours, the concentration of extract mouthwash The lowest lime peel was 15% = 8.83 mm, the highest inhibition zone was at a concentration of 50% = 10.50 mm, 25% concentration = 9.80 mm, the control group Formula Mouthwash Biomedical Laboratory Concoction of the Faculty of Medicine UNISSULA = 10, 13mm. Lime peel extract mouthwash is feasible and effective in inhibiting streptococcus mutans which causes caries.

Metabolomic Analysis of Phytochemical Compounds from Ethanolic Extract of Lime (Citrus aurantifolia) Peel and Its Anti-Cancer Effects against Human Hepatocellular Carcinoma Cells.

Lime peels are food waste from lime product manufacturing. We previously developed and optimized a green extraction method for hesperidin-limonin-rich lime peel extract. This study aimed to identify the metabolomics profile of phytochemicals and the anti-cancer effects of ethanolic extract of lime (Citrus aurantifolia) peel against liver cancer cells PLC/PRF/5. The extract's metabolomics profile was analyzed by using LC-qTOF/MS and GC-HRMS. The anti-cancer effects were studied by using MTT assay, Annexin-PI assay, and Transwell-invasion assay. Results show that the average IC50(s) of hesperidin, limonin, and the extract on cancer cells' viability were 165.615, 188.073, and 503.004 µg/mL, respectively. At the IC50 levels, the extract induced more apoptosis than those of pure compounds when incubating for 24 and 48 h (p < 0.0001). A combination of limonin and hesperidin showed a synergistic effect on apoptosis induction (p < 0.001), but the effect of the combination was still less than that of the extract at 48 h. Furthermore, the extract significantly inhibited cancer cell invasion better than limonin but equal to hesperidin. At the IC50 level, the extract contains many folds lower amounts of hesperidin and limonin than the IC50 doses of the pure compounds. Besides limonin and hesperidin, there were another 60 and 22 compounds detected from the LCMS and GCMS analyses, respectively. Taken altogether, the superior effect of the ethanolic extract against liver cancer cells compared to pure compound likely results from the combinatorial effects of limonin, hesperidin, and other phytochemical components in the extract.

THE EFFECT OF KAFFIR LIME PEEL EXTRACT (Citrus hystrix) ON SPATIAL MEMORY OF MICE WITH DEMENTIA USING MORRIS WATER MAZE

Background: Antioxidants are a group of chemicals that protect biological systems against the potential harmful effects of oxidation processes or its reactions. Kaffir lime peel (Citrus hystrix) contains various potential antioxidants substances those are able to protect neurons from free radical-induced damage. Objective: To evaluate the effect of kaffir lime peel extract (Citrus hystrix) on the spatial memory of mice induced by Scopolamine (SCM). Methods: This research was a true experimental study with a post-test-only controlled group design. Thirty mice were randomly divided into 5 groups. SCM was injected intraperitoneally on day 1-7 and kaffir lime peel extract was given orally on day 2-7, the spatial memory testing of mice using Morris Water Maze on day 8. Data analysis used One-Way ANOVA test (α = 0.05) and continued with Post Hoc LSD test (α = 0.05). Results: The mean spatial memory of K+, K-, P1, P2,P3 were 16,33 ± 7,42; 70,00 ± 32,24; 25,66 ± 24,43; 23,00 ± 19,39; 25,00 ± 19,50 seconds respectively. There were significant differences in spatial memory in P1, P2, dan P3 to the negative control group, but there is no significant differences between treatment grups. Conclusion: Kaffir lime peel extract (Citrus hystrix) can improve the spatial memory of scopolamine-induced dementia (SCM) mice. There is no dose effect relationship between the treatment groups

Formulation and Evaluation of Lime Peel Extract (Citrus aurantiifolia) Gel with Semi-Refined Carrageenan and Glucomannan as Gelling Agent

Lime peel extract contains quercetin with sunscreen activity. It could make into gel preparations, which has a transparent semisolid and generally gives a cooling sensation to the skin. A combination of semi-refined carrageenan and glucomannan as a gelling agent can be an alternative to form the strength and elasticity of the gel mass. This study aimed to determine the effect of concentration variations of gelling agents on the physical evaluation of gel preparation results and the optimal formulation for lime peel extract gel as sunscreen. Lime peel extract would make into gel preparation with variations in the concentration of the gelling agent, which are 1%, 1.25%, 1.5%, and 1.75%. Afterward, the physical evaluation included organoleptic, homogeneity, pH, viscosity, spreadability, and adhesion tests. The organoleptic, homogeneity, pH, and adhesion test results show that all formulations meet the requirements. Viscosity test results obtain the best formula III with 3940,00 cPs at 60 rpm . The results of the dispersibility test show the best formulas I and II with 6,10 cm and 5,50 cm. Based on the data analyzed, variations of gelling agents affect the pH, viscosity, spreadability, and adhesion test results. The optimal lime peel extract gel formulation has not defined from the physical evaluation results.

Development of conductive textile fabric using Plackett–Burman optimized green synthesized silver nanoparticles and in situ polymerized polypyrrole

ABSTRACT Electronic textiles (e-textiles) are undergoing rapid technological advancements to attain e-textiles that look and feel like conventional textile fabrics. Research seeks to develop highly functionalized textile-based sensors, actuators, and energy storage devices that integrate seamlessly with current textile technologies. Presently, developments are limited by either low electrical performance, or high cost and complex construction. Additionally, negotiating the balance between high performing e-textiles and environmentally benign production is a challenge. In this report, green synthesized silver nanoparticles (AgNPs) are composited with the conjugated polymer, polypyrrole (Ppy), to create a low-cost conductive textile fabric. A Plackett–Burman design of experiment was used to optimize lime peel extract (LPE) mediated reduction for the synthesis of AgNPs. The results of this optimization process revealed silver nitrate concentration to be a significant factor in both size and UV-vis absorption maxima of the LPE-synthesized AgNPs, and reaction temperature also affecting UV-vis absorption maxima. The resultant optimized AgNPs were consistent in size (40–80 nm) and dispersity (PDI = 0.250). The LPE-synthesized AgNPs are used to form a AgNP-Ppy nanocomposite with a linen textile to produce an e-textile with low electrical resistance (37 Ω).

The Formulation and Analysis of Solid Bath Soap from Ethanol Extract Of Kaffir Lime Peel (Citrus hystrix DC.) With Several Concentrations

Solid bath soap is a cosmetic preparation to remove dirt from skin surfaces. Kaffir lime peel is a good natural ingredient for skin health. The aim of this study was to produce cosmetic preparation of solid bath soap from a thick extract kaffir lime peel with several concentrations. The thick extract of kaffir lime peel is made by maceration by dissolving 500 grams of powder with 96 % ethanol as solvent as much as 1,000 mL for 2x24 hours and concentrated using a rotary evaporator at 500 C. the method used in soap making is cold processing. The result of the study was a solid bath soap, smelling of fresh orange, bluish color to turquoise blue depends on the concentration of the active substance of kaffir lime extract, soft texture and homogeneous color, Formula 1 with concentration of kaffir lime peel extract of 1 % has pH 10.0, foam height of 5.0 cm, water content was 1.76% and free fatty acids was 2.51%. Formula 2 with concentration was kaffir lime peel extract of 3 % value pH 10.0, foam height of 5.0 Cm, moisture content of 1.95 % and free fatty acids was 2.51 % while formula 3 with an extract of 5 % has a pH 10.0 foam height was 5.0 cm, water content was 0.73 % and free fatty acid was 2.51 %. Solid bath soap with thick extract off kaffir lime peel meets the requirements of SNI number 3532 of 2016 for pH, and moisture content tests while the free fatty acid test does not meet these requirements of SNI

Nano antioxidant serum ethanol extract lime peel (Citrus aurantifolia S.)

Serum is a cosmetic that has received great attention from the industry, but the main challenges are appearance, transparency and public acceptance of herbal active ingredients. On the other hand, herbal cosmetics, such as those using lime peel, are also growing. This study aims to determine the antioxidant activity of lime peel made in a nano extract serum preparation. The method for making nano extracts uses ionic gelation with PSA (Particle Size Analyzer) and Zeta Potential characterization. The concentration of nano serum of lime peel ethanol extract used was 0.5%; 1%; and 3%. Meanwhile, the antioxidant activity testing method uses DPPH and data analysis applied one-way ANOVA then by Tukey test. The results of the nano extract serum formulation using organoleptic testing are transparent, thick and liquid, odorless, and feel moist on the skin. The results of nanoparticle characterization using PSA measurements with three replications were 295.1; 320.3; 385.4 nm and the results of testing the zeta potential of nanoparticles with three replications, namely 3.2; 3.4; 6.0mV. Furthermore, the results of the antioxidant activity test on the basis of the preparation formula are classified as weak (IC50 value of 156.22 μg/mL); Meanwhile, preparation formula with a concentration of 0.5%; 1%; 3% are considered very strong (IC50 value of 46.39 μg/mL; 41.06 μg/mL; 36.27 μg/mL). The results of data analysis consider significance of p value below 0.05. The conclusion is nano lime peel extract meets the requirements and the serum preparation meets the requirements and has antioxidant activity.

THE EFFECTIVENESS OF KAFFIR LIME PEEL EXTRACT (Citrus hystrix) ON PLASMA MALONDIALDEHYDE LEVEL IN DEMENTIA MICE

ABSTRACTBackground: Antioxidant is a substance that can catch and neutralize free radicals so that the process of oxidative stress can be stopped and cell damage can be avoided. Kaffir lime peel contains antioxidant compounds that have the potential to be the neuroprotective agent and can protect neurons from free radical-induced damage.Objective: To evaluate the effect of kaffir lime peel extract (Citrus hystrix) on the plasma malondialdehyde (MDA) level of mice induced by Scopolamine (SCM).Method: This research was a true experimental study with a post-test-only controlled group design. Thirty mice were randomly divided into 5 groups consisting of a healthy control (K+), Negative Control (K-), extract 5 mg/20gBW group (P1), 10 mg/20gBW group (P2), 20 mg/20gBW group (P3). SCM was injected intraperitoneally on days 1-7 and kaffir lime peel extract was given orally on days 2-7, the MDA levels testing of mice using TBARS method on day 8. Data analysis used One-Way ANOVA test and continued with Post Hoc LSD test.Results: The mean MDA levels of K+, K-, P1, P2, P3 were 4,212; 4,644; 3,481; 4,555; 4,733 nmol/mL, respectively. MDA levels of P1 were lower than K-, even though there were no statistically significant differences.Conclusion: There was no effect of administration kaffir lime peel extract (Citrus hystrix) on MDA levels in mice with scopolamine-induced dementia.Keywords: Citrus hystrix, malondialdehyde, dementia, mice