Ligurian Sea Research Articles

Temporal evolution of plankton and particles distribution across a mesoscale front during the spring bloom

AbstractThe effect of mesoscale features on the distribution of planktonic organisms are well documented. Yet, the interaction between these spatial features and the temporal scale, which can result in sudden increases of the planktonic biomass, is less known and not described at high resolution. A permanent mesoscale front in the Ligurian Sea (north‐western Mediterranean) was repeatedly sampled between January and June 2021 using a SeaExplorer glider equipped with an Underwater Vision Profiler 6 (UVP6), a versatile in situ imager. Both plankton and particle distributions were resolved throughout the spring bloom to assess whether the front was a location of increased zooplankton concentration and whether it constrained particle distribution. Over the 5 months, the glider performed more than 5000 dives and the UVP6 collected 1.1 million images. We focused our analysis on shallow (300 m) transects, which gave a horizontal resolution of 900 m. About 13,000 images of planktonic organisms were retained. Ordination methods applied to particles and plankton concentrations revealed strong temporal variations during the bloom, with a succession of various zooplankton communities. Changes in particle abundance and size could be explained by changes in the plankton community. The front had a strong influence on particle distribution, while the signal was not as clear for plankton, probably because of the relatively small number of imaged organisms. This work confirms the need to sample both plankton and particles at fine scale to understand their interactions, a task for which automated in situ imaging is particularly adapted.

The role of atmospheric aerosols on severe convective precipitation in a Mediterranean coastal region

Liguria, located in the Northwest of Italy, is one of the Italian regions hit in the recent past by some of the most severe precipitation events among the ones observed in Italy. From a synoptic point of view, similar configurations characterize the extreme events that affected Liguria, i.e. the simultaneous presence of a deep pressure minimum west of the region and a strong high pressure over eastern Europe. Such conditions are favorable to the triggering of a quasi-stationary mesoscale V-shaped convective system over the Ligurian Sea. Furthermore, this kind of configuration is favorable to the formation and intrusion of wide plumes of aerosol, mainly mineral dust from the Sahara Desert and sea salt aerosols generated under high wind conditions in the Mediterranean basin. The present study aims at evaluating the impact that these aerosol plumes can have on meteorological fields during the triggering and evolution of the deep convective systems responsible for the Liguria flooding events. This study is carried out through numerical simulations performed with the WRF-Chem model, version 4.0. In particular, our main aim is to investigate the influence that the so-called direct (aerosol-radiation) and indirect (aerosol-cloud) interactions may have on mesoscale V-shape convective systems and on the associated rainfall events. In the simulations in which the direct aerosol effects are switched on, a consistent weakening signal is identified in the wind speed in all simulations; a major role seems to be played by the increase of downward longwave radiation in the presence of aerosol, that, by reducing the land-sea temperature gradient, causes the weakening of surface winds. More complex are the consequences of aerosol on the amount/intensity of the resulting precipitations. Unexpectedly, a reduced convergence is not necessarily associated with a weakening of precipitation and in half of the studied cases the interaction between aerosol, moisture and radiation increases the instability despite the reduced dynamical forcing due to the weakening of the convergence.

Large-Scale Re-Implantation Efforts for Posidonia oceanica Restoration in the Ligurian Sea: Progress and Challenges

The Ligurian Sea (NW Mediterranean) has been a focal point for numerous interventions aimed at restoring Posidonia oceanica meadows. The success of pioneer restoration actions in France during the 1970s stimulated similar initiatives across the Mediterranean Sea. Early attempts in the Ligurian Sea were implemented in 1993 and 1996 on limited seabed areas (i.e., tens of square meters) at the two coastal sites of Sori and Rapallo (Liguria, NW Italy). No further initiatives have been reported for the Ligurian Sea until 2022. In that year, a large-scale restoration project, which uses biodegradable mats coupled with metal mesh, began in Liguria. Different levels of anthropogenic pressure and wave exposure characterize the three investigated locations: (1) Portofino, on the eastern Liguria and on the border with the Portofino Marine Protected Area; (2) Bergeggi in the central Liguria and within the Isola di Bergeggi Marine Protected Area; and (3) Sanremo in the western Liguria, without any formal protection. Despite recent setbacks caused by severe storms in late 2023, which particularly damaged the Portofino site, ongoing monitoring revealed promising survival rates. Most notably, the site in Bergeggi displayed a 90% survival rate in September 2023. Although challenges to restore P. oceanica beds persist, such as mitigating damages caused by unpredictable events, this extensive re-implantation initiative offers the opportunity to evaluate the effectiveness of new basin-scale restoration strategies. This approach marks an important step in the conservation of Posidonia oceanica habitat.

A Beacon in the Dark: Grey Literature Data Mining and Machine Learning Enlightening Historical Plankton Seasonality Dynamics in the Ligurian Sea

The Mediterranean Sea, as one of the world’s most climate-sensitive regions, faces significant environmental changes due to rising temperatures. Zooplankton communities, particularly copepods, play a vital role in marine ecosystems, yet their distribution dynamics remain poorly understood, especially in the Ligurian Sea. Leveraging open-source software and environmental data, this study adapted a methodology to model copepod distributions from 1985 to 1986 in the Portofino Promontory ecosystem using the Random Forest machine learning algorithm to produce the first abundance and distribution maps of the area. Five copepod genera were studied across different trophic guilds, revealing habitat preferences and ecological fluctuations throughout the seasons. The assessment of model accuracy through symmetric mean absolute percentage error (sMAPE) highlighted the variability in copepod dynamics influenced by environmental factors. While certain genera exhibited higher predictive accuracy during specific seasons, others posed challenges due to ecological complexities. This study underscores the importance of species-specific responses and environmental variability in predictive modeling. Moreover, this study represents the first attempt to model copepod distribution in the Ligurian Sea, shedding light on their ecological niches and historical spatial dynamics. The study adhered to FAIR principles, repurposing historical data to generate three-dimensional predictive maps, enhancing our understanding of copepod biodiversity. Future studies will focus on developing abundance distribution models using machine learning and artificial intelligence to predict copepod standing crop in the Ligurian Sea with greater precision. This integrated approach advances knowledge of copepod ecology in the Mediterranean and sets a precedent for integrating historical data with contemporary methodologies to elucidate marine ecosystem dynamics.

On the biology of blackbellied angler, Lophius budegassa Spinola, 1807, in the north-western Mediterranean

In the Mediterranean Sea, the blackbellied angler, Lophius budegassa Spinola, 1807, represents a relevant fraction of the commercial by-catch of bottom trawling and small-scale fisheries that use passive gears (e.g., set nets, bottom longlines, etc.). Despite its importance, there are no comprehensive studies on the population dynamics of this species, and there is still scarce and scattered knowledge of its biology and ecology. Biomass, morphometric, and biological data of the blackbellied angler were collected from 1994 to 2021 by means of the Mediterranean International Bottom Trawl Survey (MEDITS) programme in the Ligurian and northern Tyrrhenian Seas, and in the EU Data Collection Framework during the period of 2006-2021. Furthermore, ageing was performed on specimens collected from 2013 to 2021 during the MEDITS programme. There were high values for the density indices and the spatial distribution of biomass in the central sector of the investigated area (the Tuscan Archipelago), mostly on the continental shelf. The length-weight relationship showed negative allometric growth when considering the sexes together, and no difference between the sexes. The size at first maturity (L50) was estimated at 39.4 cm total length (TL) in females and at 29.2 cm TL in males. The obtained Von Bertalanffy growth parameters were: in females, L∞ = 74.50 cm, k = 0.10 years-1, and t0 = -0.40 years; in males, L∞ = 61.30 cm, k = 0.14 years-1, and t0 = -0.38 years. There were differences in growth between males and females for L∞ (χ2 = 10.04; p < 0.05) and k (χ2 = 6.12; p < 0.05).

Learning from the history of red shrimp fisheries in the Mediterranean to improve sustainability of deep-water bottom trawling

Abstract As deep-water red shrimp (DWRS) trawl fisheries gain importance in the Mediterranean, their impact on fragile deep-sea ecosystems requires careful management. We reviewed the historical development of DWRS fisheries in the Mediterranean to propose a harvest strategy that would enhance fishery sustainability, while preserving habitat and community integrity. We considered two representative typologies of DWRS fisheries: a domestic fleet operating within the Italian territorial waters of the Ligurian Sea, and a distant fleet operating mostly in international waters of the central-eastern Mediterranean and sharing shrimp stocks with non-European countries. Our proposed management approach is based on three main harvesting measures: (i) preventing the uncontrolled growth in number of trawlers, (ii) adopting a catch control regime, and (iii) identifying a network of deep-water areas designated alternately for fishing and conservation purposes. As with fisheries in other regions, new fishing grounds should be explored using “encounter protocols” to avoid areas hosting species indicators of Vulnerable Marine Ecosystems. Additionally, empirical indicators such as catch rate thresholds derived from fishers’ experience, could be used to move from one fishing ground to another to avoid local depletion of DWRS abundance and a decrease in fishery profitability.

The Changing Biogeography of the Ligurian Sea: Seawater Warming and Further Records of Southern Species

Global warming is causing poleward expansion of species ranges. Temperate seas, in particular, are undergoing a process known as ‘tropicalisation’, i.e., the combination of sea-water warming and establishment of southern species. The Ligurian Sea is one of the coldest sectors of the Mediterranean and has thus been characterized by a dearth of warm-temperate species and a comparative abundance of cold-temperate species. This paper uses a time series of sea surface temperature (SST) and new records of thermophilic fish species to reconsider the biogeography of the Ligurian Sea. SST has risen by about 0.7 °C on average between 1948 and 2023, but two phases may be distinguished: a cool one (ended in the mid-1980s) and a warm one (still ongoing); the latter phase shows alternating periods of rapid warming and comparatively stationary temperature. The arrival of thermophilic species coincided with the periods of rapid warming; some of these species were established in the subsequent stationary periods. Heatwaves and climate-related diseases associated with the periods of rapid warming have caused mass mortalities of autochthonous species. Our knowledge on the biogeography of the Ligurian Sea was established during the cool phase; the present situation, however, calls for re-defining the chorological spectrum of the Ligurian Sea biota.

Where and when has the rare Fish Species Regalecus glesne been Found in the Mediterranean so far?

The finding of two specimens of oarfish, Regalecus glesne, in the north-western Ionian Sea (central Mediterranean), in the years 2021 and 2022 and at depths of 525 and 350 m respectively, is reported to update the current information on the distribution of this teleost fish in the Mediterranean waters. In fact, to date, that most of the R. glesne findings are distributed in the western part of the Mediterranean basin, between the Ligurian Sea (Italy) and the Gulf of Lion (France), with 22 and 17 findings, respectively. The TL recorded in the Ligurian Sea ranges from 86.5 to 433 cm, and in the Gulf of Lion from 88 to 446 cm. For the two captured specimens during these surveys, the estimated total length were 650 and 2700 mm, respectively. Macroscopic examination of the gonads identified these specimens as an indeterminate and a female in the early stage of gonad maturity.

A Tale of Two Sisters: The Southerner Pinna rudis Is Getting North after the Regional Extinction of the Congeneric P. nobilis (Mollusca: Bivalvia)

In the Mediterranean Sea, the bivalve genus Pinna is represented by two species: the endemic Pinna nobilis and the (sub)tropical Atlantic Pinna rudis. P. rudis is generally less common and mostly restricted to the warmer regions of the western Mediterranean. However, since a mass mortality event, caused by a pathogen infection, has brought P. nobilis to the brink of extinction, records of P. rudis have increased in several Mediterranean regions, where it had not been previously observed. This paper reports on the presence of several P. rudis individuals in the Ligurian Sea, the northernmost reach of this species in the western Mediterranean. P. rudis has become increasingly common between 2021 and 2023, with a total of 28 new records from seven localities along the Ligurian coast. The size of the individuals and their estimated growth rate (3.6 cm·a−1) indicated that a recruitment event most likely took place in summer 2020, when P. nobilis was no longer present in the area. Our observations suggest that the recruitment success of P. rudis increased following the decline of P. nobilis. However, considering the thermophilic nature of P. rudis, in all likelihood, the ongoing water warming is playing a crucial role in the successful establishment of this species in the Ligurian Sea. A full understanding of the recent range expansion of P. rudis in the Mediterranean is far from being achieved, and whether P. rudis will be able to fulfil the ecological role of P. nobilis is difficult to predict. Large scale monitoring remains the only effective way to know about the future of Pinnids in the Mediterranean Sea.

Paleoclimatic reconstruction of the past 28 kyr BP based on calcareous nannofossil assemblages in the Ligurian Sea.

Paleoclimatic reconstruction of the past 28 kyr BP based on calcareous nannofossil assemblages in the Ligurian Sea.

Post-mortem examination on a striped dolphin (Stenella coeruleoalba) reveals a potential fatal interaction with bottlenose dolphins (Tursiops truncatus) in Italian waters

Abstract Despite attacks of bottlenose dolphins towards conspecifics and other species of dolphins being reported worldwide, inside the Mediterranean Sea these behaviours are still considered sporadic and have been, to date, only recorded in Spain and France, mostly directed to striped dolphins. In this work, we reported the necropsy outcomes of a dead striped dolphin that suggest a fatal interaction with bottlenose dolphins inside the Italian side of the Pelagos Sanctuary, Western Ligurian Sea. Results from the post-mortem examination showed multiple, multifocal external rake marks spaced 1–1.2 cm and several internal injuries, consisting of subcutaneous and muscular haematomas and haemorrhages, multiple vertebral and rib fractures, haemothorax and lung laceration. The inter-tooth distance and all the internal findings were consistent with a fatal traumatic interaction with bottlenose dolphins as reported in similar cases in other parts of the world where the same results were found. No other significant gross and microscopic findings and concurrent significant pathogens were detected. This case highlights the existence of negative interspecific interactions in an area where they have never been reported before and adds new important information to the literature for understanding their occurrence, expanding the geographical range of observation in the western Mediterranean Sea.

Aemila Scauri Aurelia

Can a road, with its branches, be considered sustainable and be so important for a territory that it has always influenced its evolution and that of its inhabitants over the centuries? The main purpose of the research is to clarify how a road, via Aemilia Scauri – Aurelia, can become a symbol of culture and social identity of a territory, the Tigullio, through the centuries and millennia, investigating the visible material remains of the Roman and of medieval times. The Tigullio land is a part of Liguria in the so-called Levante Ligure, east of Genoa; enclosed between the sea and the mountains, this strip of land overlooks the Golfo del Tigullio which is part of the Ligurian Sea. Its landscape is predominantly mountainous, a feature that has always influenced the inhabitants, settlements, and roads. A complex study due to the paucity of archaeological finds, destroyed or buried under centuries of reconstruction, and the almost total absence of quotations from ancient scholars. Other problems are related to the lack of memories of the inhabitants of the area as they are mostly unaware of their historical past. Few ancient structures to understand how a road can be defined as important and sustainable for the people who have inhabited the area over the centuries, it is necessary to retrace the ancient route, identifying the few signs of the past that are still visible.

Edible Sea urchin (Paracentrotus lividus) larval dispersal and connectivity modelling in the northwestern Mediterranean Sea

The edible sea urchin (Paracentrotus lividus) is of significant economic importance due to high demand for its consumption in various Mediterranean countries. This echinoderm plays a crucial ecological role by regulating the dynamics of seaweed and seagrass through its grazing behaviour. The abundance of sea urchin is variable in time and space, but it is currently potentially declining in the Mediterranean Sea, and likely to be negatively affected by global change. Therefore, gaining a more comprehensive understanding of sea urchin larval dispersal patterns over an extended period could contribute to our understanding of how their dynamics relate to population connectivity. To investigate the trajectories of larval dispersal of this sea urchin in the Northwestern Mediterranean Sea, we combined a Lagrangian model (Ichthyop) with a hydrodynamic model (MARS3D). This study covered the years 2010–2018 and aimed to quantify the connectivity between the preferred habitats of the sea urchin (seagrasses and rocky substrates). The present study explores seasonal variations in dispersal routes and habitat connectivity relationships for two spawning scenarios identified in the literature (spring and autumn). The results enable us to identify dispersal routes, and their regularity over time. They highlight a consistent connection between Sardinia, Corsica, and the coastlines of the Ligurian and Tyrrhenian Seas, indicating a lack of genetic structuring within the edible sea urchin population in this area, particularly in Corsica. The connectivity matrices confirm the observed dispersal trajectories and highlight certain areas of significant and enduring local retention over time. The results also reveal minimal seasonal variability. Conversely, interannual variability appears pronounced, particularly in relation with trends in the warming of surface waters in the Mediterranean, which seem to impact the dispersal routes taken by P. lividus larvae. Lastly, a connectivity map was created to explore spatial resource management perspectives, aiming to identify frequent connections over an extended time period and assign varying levels of intensity to them. This approach can provide guidance with regard to resource management issues for different coastal areas that can be connected by larvae.

Optical Constituent Concentrations and Uncertainties Obtained for Case 1 and 2 Waters From a Spectral Deconvolution Model Applied to In Situ IOPs and Radiometry

AbstractA spectral deconvolution model (SDM) for inversion of light absorption, a(λ) and backscattering, bb(λ), to estimate concentrations of chlorophyll (CHL), colored dissolved organic material (CDOM) and non‐biogenic mineral suspended solids (MSS) in offshore and shelf waters is presented. This approach exploits the spectral information embedded in the ratio bb(λ)/a(λ), without the need to know each parameter separately. The model has been applied to in situ inherent optical properties (IOPs), a(λ) and bb(λ), and to in situ remote sensing reflectance, rrs(λ). CHL, MSS, and CDOM estimates are provided by propagating uncertainties in input IOPs and material‐specific IOPs using a bootstrapping approach. Application of the SDM to a data set collected in the Ligurian Sea provides Mean Average Errors (MAE) of <0.7 mg m−3 for CHL, <0.02 m−1 for CDOM, and <0.2 g m−3 for MSS. The SDM is found to perform as well as, or in some cases better than, single parameter algorithms and other semi‐analytical algorithms (SAA) for each parameter for the Ligurian Sea data set. The SDM CHL product is tested using the NOMAD, Case 1 dominated, global data set and found to perform consistently with the quasi‐analytical algorithm (Lee et al., 2002, https://doi.org/10.1364/ao.41.005755) but with slightly poorer performance than standard OCx algorithms. However, the additional estimates of CDOM and MSS provided by the SDM suggest that the approach may be particularly useful for Case 2 waters. Successful retrieval of constituent concentrations with uncertainties suggests good potential to adapt this technique for satellite remote sensing.

The comparative study by Raman spectroscopy of the plastic tide in the three ports of the Mediterranean Sea

This paper summarizes the field studies on marine microplastics (MPs) carried out in the autumn season in four various localisations within three ports chosen at the Mediterranean Sea near the French Riviera and the West Coast of Italy (within the Ligurian Sea). It considers the transport problem and the fate of the MPs introduced to the sea by analysing beach debris found on the shore after the stormy weather. Monitored ports included Saint-Tropez, Portoferraio and Porto Ercole, in which two different places were monitored. The aim is to approach the plastic tide phenomena by concentrating on a selected fraction of all MPs presented on the seashore. The final identification of debris was performed using Raman spectroscopy, providing a high-resolution signal. The PE, PP and PS contents were compared as the most frequent and representative polymers. Finally, we tackle the pending issue of the compound leakage from the MPs taking the environmentally aged particles from Portoferraio for further laboratory experiments and discuss an innovative approach with a low detection limit based on the electrochemical methods.Graphical

Relative dispersion and kinematic properties of the coastal submesoscale circulation in the southeastern Ligurian Sea

Abstract. An array of Lagrangian instruments (more than 100 drifters and a profiling float) were deployed for several days in the coastal waters of the southeastern Ligurian Sea to characterize the near-surface circulation at the submesoscale (< 10 km). The drifters were trapped in an offshore-flowing filament and a cyclonic eddy that developed at the southwestern extremity of the filament. Drifter velocities are used to estimate differential kinematic properties (DKPs) and the relative dispersion of the near-surface currents on scales as small as 100 m. The maximum drifter speed is ∼ 50 cm s−1. The DKPs within the cluster exhibit considerable spatial and temporal variability, with absolute values reaching the order of magnitude of the local inertial frequency. Vorticity prevails in the core of the cyclonic eddy, while strain is dominant at the outer edge of the eddy. Significant convergence was also found in the southwestern flow of the filament. The initial relative dispersion on small scales (100–200 m) is directly related to some of the DKPs (e.g., divergence, strain and instantaneous rate of separation). The mean squared separation distance (MSSD) grows exponentially with time, and the finite-size Lyapunov exponent (FSLE) is independent of scale. After 5–10 h of drift or for initial separations greater than 500 m, the MSSD and FSLE show smaller relative dispersion that decreases slightly with scale.

Dynamics of Atmospheric-Driven Surface Currents on The Gulf of Cadiz Continental Shelf and its link with The Strait of Gibraltar and The Western Alboran Sea

Several studies about the coastal circulation over the continental shelf of the Gulf of Cadiz have been published in the last twenty years. One of the most studied features in these papers are the so-called Coastal Counter Currents (CCCs) which are located roughly between the 100 m isobath and the coastline and are directed westwards. Despite the considerable number of articles devoted to the analysis of this phenomenon, the actual along-coast extension and origin of these CCCs currently remain unclear.In the present study, different mechanisms are proposed to explain the development of these CCCs. They have been revised and discussed taking into account the latest findings from a recent analysis that utilized surface currents derived from High Frequency Radar (HFR) as well as numerical model simulations. The most interesting finding is that the most intense CCCs events are initiated on the coastal margin of the westernmost side of the Strait of Gibraltar, and they are extended further west of Cape Santa Maria. Furthermore, these intense and extensive CCCs seem to be triggered by the joint action of local winds over the Gulf of Cadiz and additional remote effects which are coming from (i) the wind forcing over the Alboran Sea and the easternmost side of the Strait of Gibraltar, and (ii) the Sea Level Atmospheric Pressure forcing over the Ligurian Sea (western Mediterranean Sea). In addition, the same forcing mechanisms are valid to explain the eastward intensification of the coastal currents.The most relevant feature of the hydrodynamic pattern originated by this combination of forcing agents is its quasi-geostrophic adjustment against the coast in the whole spatial dominion. In addition, these results offer a clear example of how important the effects of forcing in rather remote regions may be in defining the hydrodynamic patterns of certain oceanographic regions.

An Epidemiological Update on Anisakis Nematode Larvae in Red Mullet (Mullus barbatus) from the Ligurian Sea.

Red mullet (Mullus barbatus) is a commercially relevant fish species, yet epidemiological data on anisakid nematode infestation in M. barbatus are scarce. To fill this gap, we report the occurrence of Anisakis larvae in red mullet in the Ligurian Sea (western Mediterranean). This survey was performed between 2018 and 2020 on fresh specimens of M. barbatus (n = 838) from two commercial fishing areas (Imperia, n = 190; Savona, n = 648) in the Ligurian Sea. Larvae morphologically identified as Anisakis spp. (n = 544) were characterized using PCR-RFLP as Anisakis pegreffii. The overall prevalence of A. pegreffii was 24.46%; the prevalence at each sampling site was 6.32% for Imperia and 29.78% for Savona. Furthermore, 3300 larvae of Hysterothylacium spp. were detected in the visceral organs of fish coinfected with A. pegreffii, showing that coinfection with two parasitic species is not rare. This study provides a timely update on the prevalence of ascaridoid nematodes in red mullet of the Ligurian Sea, an important commercial fishing area in the Mediterranean.

Plastitar records in marine coastal environments worldwide from 1973 to 2023

Plastics embedded in tar residues encrusting rocky coastlines were discovered on the Canary Islands (NE Atlantic Ocean) and termed “plastitar” in 2022. As plastic and tar pollution is widespread in marine pelagic and benthic habitats, it was predicted that this novel plastic form could occur on other coasts as well. To test this prediction, we reviewed the literature on plastic embedded in tar and examined rocky coastlines in our regions for plastitar. We found eight studies which reported plastic embedded in tar under several descriptions and terms (including “tar-bonded beach-conglomerate tarcrete”, “plasto-tar crust”, “plasto-tarball” and “plastitar”) in India (Arabian Sea), Bermuda (Saragossa Sea), the Bahamas (NW Atlantic Ocean), the United Arabic Emirates (Arabian Gulf and Gulf of Oman), Malta (central Mediterranean Sea), Croatia (Adriatic Sea), Italy (Adriatic and Ligurian Sea), Cabrera island, Menorca island and Sardinia island (western Mediterranean Sea) and Indonesia (Java Sea) from 1973 to 2023. We also detected plastitar during our field surveys in Japan (Sea of Japan) and Mallorca island (western Mediterranean Sea) in 2022 and 2023, respectively. Using Fourier-transform infrared spectroscopy (FTIR), we verified the contained plastics as polyethylene (PE). Pyrolysis-gas chromatography-mass spectrometry (PY-GC/MS) indicated that the tar residues derived from crude oil. Furthermore, plotting all these plastitar records in a global map revealed that almost all plastitar findings were made along the major oil transportation routes across the northern hemisphere suggesting that plastitar resulted from tanker-released crude oils. Overall, our study shows, for the first time, that plastitar has been a widespread and long overlooked plastic form in coastal systems worldwide.

Monitoring and Genotyping of Norovirus in Bivalve Molluscan Shellfish from Northern Italian Seas (2018-2020).

Norovirus (NoV) is an enteric virus with foodborne transmission. Bivalve shellfish are a main source of infections and outbreaks. In Italy a voluntary based monitoring plan to check the safety of bivalve shellfish was set up at provincial level. This study describes the occurrence and distribution of NoV in the Northern Adriatic Sea and in the Ligurian Sea. From October 2018 to September 2020, 807 bivalve shellfish samples (n = 205 oysters, n = 182 mussels, n = 348 clams, n = 72 other bivalve shellfish) were tested by One-Step Retrotranscription Real-time polymerase chain reaction for NoV GI and GII and quantified according to the ISO 15216-2:2013 and ISO 15216-1:2017. Positive samples were further analyzed to determine genotype by sequencing of the ORF1/ORF2 junction of the viral genome. A total of 126 samples were positive for NoV, mussels, and oysters had the highest probability of being positive and positive samples were found mainly in the colder season. Of these samples, 46% were NoV GII, 13% NoV GI, and 40% carried both genogroups. Thirty-seven samples were typeable (GI n = 12 and GII n = 25) with GI samples belonging to four genotypes and GII samples belonging to five genotypes. GII.3 genotype was the most prevalent, followed by GII.4, particularly Sydney 2012 subtype, a leading cause of infections worldwide, was found in three oysters' and three clams' samples. The phylogenetic analysis revealed a high heterogeneity among the species that are scattered in several clusters. Considering the low infectious dose the overall presence of NoV in edible shellfish, particular those to be eaten raw or undercooked, is moderately high. The presence of genotypes frequently involved in human infections strengthens the need for ongoing monitoring, which should be extended at national level.