Ligularia Fischeri Research Articles

Growth characteristics of the Ligularia fischeri cultivated under different light conditions in hardwood forests in central Korea

The cultivation of wild greens under the conventional culture system has been increasing in Korea, but the production cost is high and the quality of the products’ flavor and aroma is not as good as that of uncultivated wild plants. Thus, cultivation of wild greens in a forest farming system is an attractive alternative to wild harvesting, due to its much lower production cost compared with conventional cultivation, and its provision of a second income to the landowner. Yet little is known about the conditions that would maximize the growth of wild greens in a forest farming system. Thus, this study was conducted to evaluate the optimal conditions that would maximize the yield of Ligularia fischeri (Ledeb.) Turcz., which is being cultivated in three forest farming systems in Korea. After the fibrous roots of L. fischeri were planted in hardwood forests, the environmental conditions therein were measured, including the relative light intensity, soil moisture content, and plant growth characteristics such as the survival rate, total number of leaves, and leaf width. The results showed that the light intensity and tree density in such sites were significantly correlated with the leaf growth. It is thus suggested that the 20–40% light transmittance that was measured in the open field is the optimal condition for the growth and yield of L. fischeri in a forest farming system. The results of this study can help formulate a management plan for when thinning practices should be performed, to establish a forest farming system and to maintain a suitable tree density for the cultivation of wild greens.

장안산과 팔공산 구간(전북 장수)의 식물상 및 보전관리방안

The vascular plants in the trail from Jangansan to Palgongsan were listed 389 taxa (8.0% of all 4,881 taxa of Korean vascular plants); 84 families, 226 genera, 325 species, 1 subspecies, 55 varieties and 8 forms. Furthermore, hemicryptophytes (H) were 110 taxa (28.3%), geophytes (G) were 77 taxa (19.8%), megaphanerophytes (MM) were 48 taxa (12.3%), nanophanerophytes (N) were 47 taxa (12.1%) showed high proportional ratio in life form. So, resource plants of 237 taxa of edible (60.9%), 278 taxa of medicinal (71.5%), 229 taxa of ornamental (58.9%) and 178 taxa of the others (45.8%). Based on the list of rare plants, 7 taxa (1.8% of all 389 taxa of flora and 1.2% of all 571 taxa of rare plants); Paeonia japonica (VU), Viola albida, Viola diamantica, Lilium distichum, Disporum ovale, Tricyrtis dilatata, Iris ensata var. spontanea (LC), etc. Based on the list of endemic plants, 7 taxa (1.8% of all 389 taxa of flora and 2.1% of all 328 taxa of endemic plants); Salix koriyanagi, Aconitum pseudolaeve var. erectum, Thalictrum actaefolium var. brevistylum, Philadelphus schrenckii, Asperula lasiantha, Weigela subsessilis, Carex okamotoi, etc. Based on the list of specific plants, 46 taxa (11.8% of all 389 taxa of flora and 4.3% of all 1,071 taxa of specific plants); 2 taxa (Wisteria floribunda for. floribunda, Cardamine yezoensis, etc.) in class IV, 6 taxa (Acer palmatum, Ulmus davidiana, etc.) in class III, 14 taxa (Ligularia fischeri, Cymopterus melanotilingia, etc.) in class II, 24 taxa (Ilex macropoda, Fraxinus mandshurica, etc.) in class I. Based on the list of naturalized plants, 6 families, 16 genera, 18 taxa (Phytolacca americana, Robinia pseudoacacia, Oenothera odorata, Erechtites hieracifolia, Festuca arundinacea, etc.) and invasive alien plants were 3 taxa; Rumex acetocella, Ambrosia artemisiifolia, Aster pilosus. Naturalization rate (NR) was 4.6% of all 389 taxa of vascular plants and urbanization index (UI) was 5.6% of all 321 taxa of naturalized plants.

Far-infrared drying characteristics and quality assessment of Ligularia fischeri

The purpose of this study was to investigate drying models and drying characteristics of Ligularia fischeri by using far-infrared drying. The far-infrared drying tests on L. fischeri were performed at air velocities of 0.6 and 0.8 m/s, and drying chamber temperatures of 40, 45, and 50°C. Four thin layer drying models were used to estimate drying curves. Drying characteristics were analyzed on the basis of drying rate, color, antioxidant activity, and contents of polyphenolics and flavonoids. The goodness of the models was estimated using the coefficient of determination, the root mean square error, and the reduced chi-square. The results revealed that increases in drying temperature and air velocity caused a decrease in drying time. The Page and Thompson models were considered suitable for the far-infrared drying of L. fischeri. After drying, the antioxidant properties of L. fischeri were decreased under all drying conditions.

Terpenoid 분석을 통한 취나물류의 향기지표물질 비교

A comparison of essential oils composition of Aster tataricus L. (gaemichwi), Ligularia fischeri (gomchwi), Solidago virga-aurea var. asiatica Nakai (miyeokchwi), and Aster scaber (chamchwi) was performed by gas chromatography and mass spectrometry for the identification of volatile flavor characteristics in chwi-namuls. The essential oils were extracted by the hydro distillation extraction method. One hundred volatile flavor components were identified from gaemichwi essential oil. <TEX>${\alpha}$</TEX>-Pinene (11.5%) was the most abundant compound, followed by myrcene (8.9%) and <TEX>${\beta}$</TEX>-pinene (7.5%). Ninety-one volatile flavor components were identified from the essential oil of gomchwi. Aromadendrene (14.8%) was the most abundant component, followed by <TEX>${\beta}$</TEX>-caryophyllene (7.6%) and 1-methyl-4-(1-methylethylidene)-cyclohexene (7.3%). Ninety-five volatile flavor constituents were detected in the essential oil of miyeokchwi, moreover, spathulenol (15.7%) was the most abundant component. Ninety-six volatile flavor constituents were detected in the essential oil of chamchwi. Epi-bicyclosesquiphellandrene (21.9%) was the most abundant component, followed by <TEX>${\beta}$</TEX>-caryophyllene (9.5%) and <TEX>${\delta}$</TEX>-terpinene (8.9%). The essential oil composition of gaemichwi was characterized by a higher contents of pinenes. The essential oil composition of gomchwi can be easily distinguished by the percentage of aromadendrene. Spathulenol and epi-bicyclosesquiphellandrene were regarded as the characteristic odorants of miyeokchwi and chamchwi, respectively.

곰취(Ligularia fischeri)와 한대리곰취(Ligularia fischeri var. spicifoprmis) 정유의 향취 및 향기성분 비교

본 연구의 목적은 곰취(Ligularia fischeri)와 한대리곰취(Ligularia fischeri var. spicifoprmis) 정유의 향취 및 화학성분을 비교하는데 있다. 곰취와 한대리곰취의 정유 함량은 각각 0.12%와 0.04%였으며, 역치수준은 각각 0.01%와 0.1%였다. 향수개발전문가에 의해 평가된 곰취 정유의 향취는 citrus, coniferous, green, oily, smoky, wet하였던 반면 한대리곰취 정유의 향취는 balsam, earthy, oily, wet하여 매우 상이하였다. 곰취 정유에는 총 19종의 화학성분이 검출되었는데 탄화수소가 14종(75%), 알코올이 4종(20%), 아세테이트가 1종(5%)이었으며, 주된 화학성분은 L-β-pinene(36.02%), D-limonene(25.64%), α-pinene(24.85%), β-phellandrene(5.39%), 3-carene(3.77%), α-phellandrene(2.05%)이었다. 한대리곰취 정유에는 총 25종의 화학성분이 검출되었는데 탄화수소 17종(68%), 알코올 4종(16%), 아세테이트 3종(12%), 질소화합물 1종(4%)이었으며, 주된 화학성분은 D-limonene(39.80%), L-β-pinene(35.43%) α-pinene(11.94%), 3-carene(3.70%), β-cubebene(3.51%), β-phellandrene(2.38%)이었다. 한대리곰취 정유에서는 ρ-cymene, γ-muurolene, γ-cadinene, germacrene D, ingol 12-acetate, butyl 9,12,15-octadecatriene과 nimorazole이 미량 검출되었으나, 곰취 정유에서는 이러한 화학성분들이 검출되지 않았다. 본 연구의 결과 각각 독특한 향취를 갖는 곰취와 한대리곰취 정유는 향장산업제품 개발을 위한 소재로 활용될 수 있을 것이라 판단된다.

식물공장 농산물에 대한 소비자 선호속성 탐색

이 연구는 식물공장 시스템으로부터 재배된 농산물에 대한 소비자 선호속성과 지불의향을 파악하기 위해 수행되었다. 식물공장 시스템으로부터 재배되는 농산물은 상추, 딸기, 곰취, 인삼으로 구분하였다. 우리는 소비자의 구매행태와 선호를 조사하기 위해 설문지를 구성하고, 선호분석과 한계지불의사액(MWTP) 추정을 위해 최종적으로 200여개의 타당한 응답을 선택하여 분석에 이용하였다. 우리는 실증분석을 위해 컨조인트 분석과 다항로짓모형을 이용하였다. 품목별 지불의사액 추정결과, 상추 1,016원(100g), 딸기 6,014원(2kg), 곰취 3,652원(100g), 인삼 5,905원(100g)으로 나타났다. 몇몇 소비자 구매행태의 속성이 포착되어 제시되었고, 식물공장 시스템에 대한 보다 적절한 정책 수립을 위해 몇가지 정책적 함의가 제안되었다. This study was carried out to find consumers' attributes and willingness to pay(WTP) of agricultural products cultivated from plants factory system. The agricultural products were sorted into lettuce, strawberry, Ligularia fischeri(wild edible greens), and ginseng. We made the questionaires to survey consumers' purchasing attributes and preference of agricultural products. Finally, 200 valid replies were selected to analyze consumers' attributes and to estimate marginal willingness to pay. We used conjoint analysis and multinomial logit model for empirical analysis. MWTPs of commodities are estimated as follows; Lettuce is 1,016KW(100g), Strawberry is 6,014KW(2kg), Ligularia fischeri is 3,652KW(100g), and Ginseng is 5,905KW(100g). Several attributes of consumers' purchasing behavior were identified from this study. We suggested several implications to formulate more appropriate policy of plants factory system.

고랭지 경사 밭 콩 재배지에서 토양유실경감을 위한 피복방법 평가

There is relatively high vulnerability of soil erosion in slope highland agriculture due to a reclamation of mountain as well as low surface covering in early summer season with high rainfall intensity time. The aim of this study was to evaluate various surface covering methods for reducing soil loss in highland radish cultivation in highland. The experiment was conducted in 17% sloped lysimeter () with 8 treatments including covering with cut rye, sod culture of rye, Ligularia fischeri var. spiciformis Nakai, Arachniodes aristata Tindale, Aster koraiensis Nakai, Festuca myuros L. and mulching with black polyethylene film, and runoff water, eroded soil and radish growth were investigated. Surface covering with sod culture and plant residue, especially cut rye treatment, had lower runoff water than non-covering, whereas black polyethylene film mulching had the reverse. The amount of eroded soil was also lowest in cut rye treatment, , and increased in the order of rye sod culture, Ligularia fischeri var. spiciformis Nakai, Aster koraiensis Nakai, Festuca myuros L., Arachniodes aristata Tindale, black polyethylene film, and non-covering, . The results showed that surface covering with sod culture or plant residue could be effective for reducing runoff water and soil erosion in the radish field, significantly in cut rye treatment. On the other hand, in sod culture of rye, Aster koraiensis Nakai and Ligularia fischeri var. spiciformis Nakai, radish yields were lower than in the non-covering. Unlike this, covering with cut rye, sod culture of Festuca myuros L. had similar radish yield to the non-covering radish yield. In conclusion, covering with cut rye and sod culture of Festuca myuros L. were beneficial for reduction of soil loss without decreasing in radish yield in highland sloped fields.

NEW ORNAMENTAL FLOWERS IN BEIJING

Based on investigation of wild flowers in northern China 80 species of wild flowers were introduced in Beijing. After five years of research and cultivation eight kinds of ornamentals were selected as good, drought resistant, extensive management, cold-resistant, suitable for planting in the Beijing area, 5 for early spring blooming, Adonis amurensis, Pulsatilla dahurica, Corydalis ambigua var. amurensis, Corydalis speciosa, Tulipa altaica, 2 for summer, Thalictrum petaloideum and Ligularia fischeri, and 1 for fall, Sanguisorba officinalis.

Inhibition of Gastrointestinal Lipolysis by Green Tea, Coffee, and Gomchui (Ligularia fischeri) Tea Polyphenols during Simulated Digestion

Green tea, coffee, and gomchui (Ligularia fischeri) tea, which are rich in polyphenols, may exhibit antiobesity effects by inhibiting pancreatic lipase. However, the bioavailability of some polyphenols is poor due to either degradation or absorption difficulties in the gastrointestinal tract, thus making their beneficial effects doubtful. This study was conducted to evaluate the inhibitory effect of three beverages on lipolysis and the contribution of their major polyphenols during simulated digestion. During simulated digestion, gomchui tea was the most potent at inhibiting gastrointestinal lipolysis, whereas green tea was the least potent. The strongest lipase inhibitor among purified major polyphenols was a green tea polyphenol, (-)-epigallocatechin gallate (EGCG, IC(50) = 1.8 ± 0.57 μM), followed by di-O-caffeoylquinic acid isomers (DCQA, IC(50) from 12.7 ± 4.5 to 40.4 ± 2.3 μM), which are gomchui tea polyphenols. However, the stability of DCQA was greater than that of EGCG when subjected to simulated digestion. Taken together, gomchui tea, which has DCQA as the major polyphenol, showed stronger lipolysis inhibitory activity during simulated digestion compared to both green tea and coffee.

Content of Antioxidative Caffeoylquinic Acid Derivatives in Field-Grown Ligularia fischeri (Ledeb.) Turcz and Responses to Sunlight

Ligularia fischeri (Ledeb.) Turcz, a commercial leafy vegetable, contains caffeoylquinic acid derivatives (CQAs) as major phenolic constituents. The HPLC chromatograms of leaf extracts collected from different areas in Korea showed a significant variation in CQA amount, and two tri-O-caffeoylquinic acids (triCQAs) were purified and structurally identified by NMR and MS from this plant. Radical scavenging activities among CQAs were found to be increased in proportion to the number of caffeoyl groups. Since this plant prefers damp and shady growth conditions, the effects of sunlight were investigated by growing plantlets in sunlight and shade for four weeks. Greater leaf thickness and higher phenolic contents were found for leaves grown in sunlight than in shade. Four major CQAs-5-mono-O-caffeoylquinic acid (5-monoCQA), and 3,4-, 3,5-, and 4,5-di-O-caffeoylquinic acid (diCQA)-were induced by solar irradiation, whereas the content of these compounds decreased steadily in shade leaves. The leaves of L. fischeri clearly showed adaptation responses to sunlight, and these characteristics can be exploited for cultivation of this plant for potential use as a nutraceutical and functional food.

Inhibititory Effect of Gastrointestinal Lipolysis by Green Tea, Coffee, and Gomchui (Ligularia fischeri) Tea during Simulated Digestion

Green tea (GrT) and coffee are both rich in bioactive polyphenols (PP), such as EGCG or caffeoylquinic acids (CQA). PPs are beneficial to human body, especially, known to inhibit pancreatic lipase resulting in anti‐obesitic effect. Gomchui (Ligularia fischeri) has been used as herbal medicine or consumed as tea in Eastern Countries. It is also known to be rich in PPs, dicaffeoylquinic acid (DCQA). Despite of these beneficial effects of PPs, some are poor in bioavailability due to either degradation or difficulty of absorption in the GI track. Among them, GrT EGCG is easily degraded during digestion, thus making the beneficial effect doubtful in our body. We first investigated the inhibitory effect of three teas on lipid catabolism and found out Gomchui tea (GoT) was the most potent inhibitor. We assumed that the potency was due to both lipase inhibitory effect and the stability of PPs. Thus we monitored their stability during simulated digestion (SD) and assayed for the inhibitory effect on lipase. Among tested, EGCG was the most potent inhibitor (IC50 1.8 ± 0.57 μM) followed by DCQA (33.2 ± 4.2 μM). However, the stability was inverted. EGCG was not detected after the SD whereas DCQA was stable. Taken these together, it was concluded that despite of high potency on lipase inhibition, the overall effect of a PP on lipid catabolism should be considered as the sum of inhibitory effect as well as the stability during digestion.

Hepatoprotective effect of Handaeri-gomchi (Ligularia fischeri var. spiciformis Nakai) extract against chronic alcohol-induced liver damage in rats

Handaeri-gomchi (Ligularia fischeri var. spiciformis Nakai) (LF) is well known as a medicinal plant in Korea, especially to treat a variety of human disease including hepatic function failure. In this study, we examined the activity of LF extract against the liver injuries and oxidative stress by chronic alcohol in Spargue-Dawley rats. Severe liver damage caused by alcohol intake with increasing activity of hepatic markers was decreased in the group of rats fed LF extract. The results were confirmed through hematoxylin and eosin staining. Antioxidative capacity was decreased by alcohol but it was recovered by LF extract. Elevated indicators of oxidative stress by chronic alcohol were diminished in the group of LF extract. Furthermore, LF extracts increased antioxidative capacity and the activity of antioxidant enzymes. In conclusion, LF extract has a protective effect against chronic alcohol hepatotoxicity, suggesting it could be developed as a functional food or medicine for protection of liver disease.

수리취(Synurus deltoids)의 일반성분, 무기질 및 식이섬유에 관한 연구

This study was conducted to estimate the general components, minerals, and dietary fiber contents of Synurus deltoides. S. deltoides contained 81.1% moisture content, and the proportions of crude fat, crude protein, crude ash, and crude fiber were 0.3%, 4.2%, 2.6%, and 3.5%, respectively. Potassium (3,249.1 mg) was the most abundant component among the minerals in S. deltoides. In addition, S. deltoides contained many other minerals, e.g. calcium (854.8 mg), phosphorus (60.3 mg), magnesium (344.7 mg), sodium (57.3 mg), zinc (1.7 mg), iron (30.9 mg), copper (0.8 mg), and manganese (5.8 mg). Almost all of the mineral contents of S. deltoides were higher than those of Aster scaber and Ligularia fischeri, except for zinc, copper, and manganese. Total dietary fiber (TDF), insoluble dietary fiber (IDF), and soluble dietary fiber (SDF) contents of S. deltoides were 42.6 g, 37.9 g, and 4.7 g, respectively, and these were also higher than those of A. scaber and L. fischeri used in this study. These results suggest that S. deltoides may be a valuable nutrient source.

고랭지 경사 밭 무 재배지에서 토양유실경감을 위한 피복방법 평가

There is relatively high vulnerability of soil erosion in slope highland agriculture due to a reclamation of mountain as well as low surface covering in early summer season with high rainfall intensity time. The aim of this study was to evaluate various surface covering methods for reducing soil loss in highland radish cultivation in highland. The experiment was conducted in 17% sloped lysimeter () with 8 treatments including covering with cut rye, sod culture of rye, Ligularia fischeri var. spiciformis Nakai, Arachniodes aristata Tindale, Aster koraiensis Nakai, Festuca myuros L. and mulching with black polyethylene film, and runoff water, eroded soil and radish growth were investigated. Surface covering with sod culture and plant residue, especially cut rye treatment, had lower runoff water than non-covering, whereas black polyethylene film mulching had the reverse. The amount of eroded soil was also lowest in cut rye treatment, , and increased in the order of rye sod culture, Ligularia fischeri var. spiciformis Nakai, Aster koraiensis Nakai, Festuca myuros L., Arachniodes aristata Tindale, black polyethylene film, and non-covering, . The results showed that surface covering with sod culture or plant residue could be effective for reducing runoff water and soil erosion in the radish field, significantly in cut rye treatment. On the other hand, in sod culture of rye, Aster koraiensis Nakai and Ligularia fischeri var. spiciformis Nakai, radish yields were lower than in the non-covering. Unlike this, covering with cut rye, sod culture of Festuca myuros L. had similar radish yield to the non-covering radish yield. In conclusion, covering with cut rye and sod culture of Festuca myuros L. were beneficial for reduction of soil loss without decreasing in radish yield in highland sloped fields.

곰취(Ligularia fischeri), 미역취(Solidago virga-aurea), 삼나물(Aruncus dioicus) 복합 추출물의 항염증 효과

본 연구에서는 울릉도 특산 약용 작물 추출물이 항염증에 대한 실험연구가 이루어져 있지 않은 것에 착안하여 LPS에 의해 활성화된 대식세포로부터 유도되는 염증반응에 대한 억제효과를 조사하였다. 울릉도 자생 식물인 곰취, 미역취, 삼나물의 세가지 식물 추출물을 이용하여 피부 염증에 대하여 연구를 하였다. 산화질소와 cytokine의 생산은 면역세포의 대표적인 염증인자이다. 세포는 LPS 처리 후 한 시간 뒤에 곰취, 미역취, 삼나물 70% 아세톤 추출물을 처리를 하였다. 세포 독성이 나타나지 않는 농도인 1, 10, 100 ug/ml를 사용하였다. 곰취, 미역취, 삼나물 70% 아세톤 추출물은 NO, <TEX>$PGE_2$</TEX>, TNF-<TEX>${\alpha}$</TEX>, IL-1<TEX>${\beta}$</TEX>, IL-6, iNOS, COX-2의 생성을 저해 시켰다. <TEX>$PGE_2$</TEX>는 100 ug/ml의 농도에서 60%에 가까운 저해율을 나타내었다. iNOS와 COX-2 역시 100 ug/ml의 농도에서 각각 54%, 65%가 저해가 되었다. 게다가 곰취, 미역취, 삼나물 70% 아세톤 추출물은 염증성 사이토 카인인 TNF-<TEX>${\alpha}$</TEX>, IL-1<TEX>${\beta}$</TEX>, IL-6의 생성을 감소 시켰다. 이러한 결과로 곰취, 미역취, 삼나물 추출물은 염증 예방과 치료에 효과적임을 확인할 수 있었다. The objective of this study was to evaluate the skin inflammation effects of three herb mixture extracts, Ligularia fischeri, Solidago virga-aurea and Aruncus dioicus, which are from Ullung island in Korea. Regulatory mechanisms of cytokines and nitric oxide (NO) are involved in the immunological activity of Raw 264.7 cells. Tested cells were pretreated with 70% acetone extracts of Ligularia fischeri, Solidago virga-aurea and Aruncus dioicus (LSA-A) and further cultured for an appropriated time after lipopolyssacharide (LPS) addition. During the entire experimental period, 1, 10, and 100 <TEX>${\mu}g/ml$</TEX> of LSA-A had no cytotoxicity. In these concentrations, LSA-A inhibited the production of NO and prostaglandin <TEX>$E_2$</TEX> (<TEX>$PGE_2$</TEX>), tumor necorsis factor-a (TNF-a), interleukin-1<TEX>${\beta}$</TEX> (IL-1<TEX>${\beta}$</TEX>), interleukin-6 (IL-6) expression of inducible NO synthase (iNOS), and cyclooxygenase-2 (COX-2). LSA-A showed a 60% <TEX>$PGE_2$</TEX> inhibition rate at 100 <TEX>${\mu}g/ml$</TEX>. iNOS and COX-2 inhibition activities were 54%, and 65% at 100 <TEX>${\mu}g/ml$</TEX>, respectively. In addition, LSA-A extract reduced the release of inflammatory cytokines including TNF-a, IL-1<TEX>${\beta}$</TEX> and IL-6. These results suggest that LSA-A may have significant effects on inflammatory factors, and may be a potential anti-inflammatory therapeutic agent.

산나물류의 식품 화학적 성분과 전자 공여능

The composition analysis and electron donating activity of 4 kinds of Korean wild sannamuls[Cacalia firma Komar. (CFK), Aster scaber Thunb. (AST), Ligularia fischeri (Ledeb.) Turcz. (LFT), Synurus palmatopinnatifidus (Makino) Kitam., SDN)] were investigated. The Korean edible mountainous vegetable was named a sannamul or chwinamul in Korea, generally. Most of them, the characterization of the each vegetable was reported, individually without comparative research. This study was compared the proximate compositions, mineral, dietary fiber and electron donating abilities on 2,2-diphenyl-1-picrylhydrazyl (DPPH) of sannamuls. In the chemical analysis of sannamuls, CFK was shown higher value in ash (), crude fat (), carbohydrate (), potassium () and magnesium () than others. Each mineral of the 3 other samples was a similar value but phosphorus in LFT was contained . The compositions of total dietary fiber of the samples were listed CFK LFT SDN AST and the Value on DPPH radicals, LFK CFT AST SDN. These results have susggest that CFK and LFT may be useful for a funtional material to food development.

Inhibitory effects of organic solvent extracts from Korean local plants on the complement classical pathway

The study evaluated the anticomplement effects from organic solvent extracts of five Compositae plants (Ligularia fischeri (Ledeb.) Turez, Ligularia taquetii (H.Lev. & Vaniot) Nakai, Ainsliaea acerifolia Sch.Bip, Aster scaber Thunb, Aster koraiensis Nakai, Synurus deltoides Aiton) from South Korea on the classical pathway complement system. We have evaluated organic solvent extracts from five Compositae with regard to its anticomplement activity. Chloroform extracts from L. taquetii showed inhibitory activity against complement system with 50% inhibitory concentrations (IC50) values of 73.2 μg/mL. This is the first report of anticomplement activity from L. taquetii.

Determination of hyperoside and 2″-acetylhyperoside from Ligularia fischeri by high performance liquid chromatography

Ligularia fischeri and its main flavonoids, hyperoside and 2″-acetylhyperoside, posses antioxidant properties. This study was carried out to investigate the contents of hyperoside and 2″-acetylhyperoside in L. fischeri by using high performance liquid chromatography (HPLC). An HPLC–photodiode array (PDA) detection method was established for the simultaneous determination of hyperoside and 2″-acetylhyperoside in L. fischeri. Two flavonoids were successfully separated in less than 20 min using an YMC RP 18 column. The mobile phase was composed of water (A) and acetonitrile (B) with isocratic elution system (23% B) at a flow rate of 1 mL/min. Their calibration curves showed good linear regression ( r > 0.9992) within the test ranges. The method was validated for specificity, accuracy, precision, and limits of detection. The determined two compounds were well separated with a linear range of 18–180 μg/mL. The contents of hyperoside and 2″-acetylhyperoside were 0.387 ± 0.002 and 0.526 ± 0.006 mg/g in L. fischeri, respectively.

Isolation and Identification of Antioxidant Compounds from Ligularia fischeri

The plant Ligularia fischeri var. spiciformis Nakai, a well-known edible medicinal herb in Korea, has been used to treat maladies such as jaundice, scarlet fever, rheumatoid arthritis, and hepatic function failure. In this research, 4 major antioxidant compounds were detected from this plant's leaves using an on-line high-performance liquid chromatography (HPLC)-ABTS screening system, which can determine the antioxidant activity based on a decrease in absorbance at 734 nm after postcolumn reaction of HPLC-separated antioxidants with the 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radicals (ABTS(*)). In order to isolate these active compounds, a preparative HPLC was applied and their chemical structures were identified as 5-O-caffeoylquinic acid (5-CQA), 3,4-di-O-caffeoylquinic acid (3,4-DCQA), 3,5-di-O-caffeoylquinic acid (3,5-DCQA), and 4,5-di-O-caffeoylquinic acid (4,5-DCQA) by ESI/MS(n) and (1)H NMR. These 4 isomers comprised over 10% of the dried leaves, with 3,5-DCQA being the most abundant compound. The radical scavenging activity of each isomer was also evaluated simultaneously through the on-line HPLC-ABTS method, which showed 94% antioxidant activity of the ethanol extract derived from caffeoylquinic acids. Among these isomers, 3,4-DCQA contained the most strong antioxidant activity while 3,5-DCQA accounted for the highest radical scavenging capacity due to having the highest content.

곰취차로부터 라디칼 소거능을 갖는 Caffeoylquinic Acid류 화합물의 추출조건

본 연구에서는 항산화 기능이 잘 알려진 곰취로부터 곰취의 소비 및 활용도 향상을 위하여 곰취차를 제조한 후 주요 항산화물질을 탐색하고, 이들 성분의 추출율 변화를 분석하였다. 우선 온라인 항산화 장치를 통하여 곰취 추출물의 주요 항산화 성분이 5-CQA, 3,4-DCQA, 3,5-DCQA 및 4,5-DCQA로 구성된 caffeoylquinic acid류의 화합물임을 확인 할 수 있었다. 곰취차에서 이들 성분의 추출율 변화를 추출온도, 시간, 용매 및 추출 방법 등에 따라 비교 분석하였는데, 물에서의 추출율은 어느 온도에서든지 10분의 분석시간동안 증가하는 것을 관찰할 수 있었다. 특히 8oC에서의 추출율도 매우 우수하였는데, 80oC 추출율의 약 60%의 유효성분이 10분 동안 추출되는 것을 확인할 수 있었다. 에탄올 함량에 따른 곰취차의 추출율에서도 추출시간에 따라 추출율이 증가하였지만, 에탄올의 함량이 많아질수록 추출율은 감소하였는데, 특히 100% 에탄올 조건에서는 추출율이 현저히 감소하였다. 곰취차의 제조 과정 중 블렌칭 단계에서는 곰취분말로부터 약 60% 이상의 항산화성분이 물로 유출되는 것이 확인되었다. 3,5-DCQA의 경우에는 90% 이상의 손실을 나타내어, 곰취분말에서는 3,5-DCQA가 가장 많이 추출되는 반면 곰취차에서는 3,4-DCQA가 가장 추출율이 높은 항산화성분이었다. 한편 초음파 추출법은 일반 추출법에 비해 항산화 성분의 추출율에는 큰 영향을 주지 않았다. 본 연구를 통해서 곰취가 차로 만들어졌을 경우에 항산화 성분을 다량 함유하고 있고, 찬물에서도 쉽게 이용할 수 있는 건강기능차로서 그 효용성과 편리성이 매우 좋을 것으로 기대된다.