Enzymatic Hydrolysis Of Lignocellulose Research Articles

Bioconversions of cellulose—A major source of material for the biochemical industry

AbstractThe abundance and renewability of lignocellulosic materials renders them to be a promising feedstock for production of energy, food and chemicals. Enzymatic hydrolysis of lignocellulose into fermentable sugars has been extensively studied in order to reach a more efficient utilization of renewable biomass energy. Enzymatic hydrolysis of lignocellulose means degradation of cellulose to D‐glucose, but the mechanism of cellulose hydrolysis by enzymes is still not well understood.This paper deals with processes for obtaining lignocellulosic materials, for converting them into fermentables by use of acid and enzyme hydrolysis, and for fermentation of the sugars to useful products.

Technology advances for continuous compression milling pretreatment of lignocellulosics for enzymatic hydrolysis

The feasibility of a continuous compression milling pretreatment process for the enzymatic hydrolysis of lignocellulosics has been demonstrated. Pretreatment efficiency was improved significantly by adjustment of feedstock moisture content prior to milling and/or increasing the roll pressure on the feedstock. Optimum moisture contents for newspaper (24%). corn stover (17%), popular (12-20%) were determined. Sugar Yields of 48% were obtained from air-dried newspaper after six passes through even-pressure rolls and the specific energy input was 0.21kW h/lb. The Effect of roll speed on enzymatic hydrolysis improvement was constant over a roll speed range of from 30-110 ft/min (65 rpm). Enzymatic hydrolysis results from commercial-scale pretreatment of moist newspaper processed at 6 tons/h/ correlated well with laboratory mill data while energy consumption was 26% less.

A lignone-dust explosion

Alkaline potassium permanganate solution (APP) was applied to the pretreatment of corncob with a simple and effective optimization of APP concentration, reaction time, temperature and solid to liquid ratio (SLR). The optimized pretreatment conditions were at 2% (w/v) potassium permanganate with SLR of 1:10 treating for 6 h at 50 °C. This simple one-step treatment resulted in significant 94.56% of the cellulose and 81.47% of the hemicellulose recoveries and 46.79% of the lignin removal of corncob. The reducing sugar in the hydrolysate from APP-pretreated corncob was 8.39 g/L after 12 h enzymatic hydrolysis, which was 1.44 and 1.29 folds higher than those from raw and acid pretreated corncobs. Physical characteristics, crystallinity and structure of the pretreated corncob were analyzed and assessed by SEM, XRD and FTIR. The APP pretreatment process was novel and enhanced enzymatic hydrolysis of lignocellulose by affecting composition and structural features.