Light-saturated Photosynthetic Rate Research Articles

Elevated ozone reduced leaf nitrogen allocation to photosynthesis in poplar

We investigated the effects of elevated ozone (O3) concentration on leaf nitrogen (N), a key determinant of plant photosynthesis, with two clones of poplar grown in open-top chambers. We focus on the difference between mass-based leaf N concentration (Nmass) and area-based one (Narea) in their responses to elevated O3, and the allocation of N to different leaf components: photosynthetic apparatus, cell walls, and others under elevated O3 level. Our results showed that elevated O3 significantly increased Nmass, but reduced Narea and leaf mass per area (LMA). The two clones showed no difference in Nmass response to O3, but the more sensitive clone showed greater reduction of Narea and LMA due to O3. We also found positive relationships between Narea and photosynthetic parameters, e.g. light-saturated photosynthetic rate (Asat). Furthermore, elevated O3 significantly reduced photosynthetic N-use efficiency (PNUE) and leaf N allocation to photosynthetic components, while increasing N allocation to cell walls and other components. We concluded that plants invested more N in cell walls and other components to resist O3 damages at the expense of photosynthetic N. The change of N allocation in plant leaves in response to elevated O3 could have an impact on ecological processes, e.g. leaf litter decomposition.

Leaf Temperature and Vapour Pressure Deficit (VPD) Driving Stomatal Conductance and Biochemical Processes of Leaf Photosynthetic Rate in a Subtropical Evergreen Coniferous Plantation

Photosynthesis is arguably the most important biochemical process on Earth, which is dramatically influenced by environmental conditions. How environmental factors drive stomatal conductance and biochemical processes of leaf photosynthetic rate has not been sufficiently investigated in subtropical China. In this study, we analysed the effects of stomatal and biochemical parameters on the photosynthetic rate of native Masson’s pine (Pinus massoniana Lamb.) and exotic slash pine (Pinus elliottii Engelm.) in response to leaf temperature and vapour pressure deficit (VPD) in subtropical China, based on leaf gas exchange measurements in 2016. Our results showed that there was no significant difference in the light-saturated photosynthetic rate (Asat) between native Masson’s pine and exotic slash pine. The seasonal patterns of maximum rate of the carboxylation (Vcmax25) were basically consistent with seasonal patterns of Asat for both species. The positive effect of leaf temperature on Asat was mainly produced through its positive effect on Vcmax25. Leaf temperature had no significant effect on stomatal conductance. Vcmax25 and gs simultaneously affected Asat in response to VPD. Our results highlighted the importance of biochemical processes in limiting leaf photosynthetic rate in response to environmental conditions in subtropical evergreen coniferous plantations.

Conifers but not angiosperms exhibit vulnerability segmentation between leaves and branches in a temperate forest

Vulnerability segmentation (VS), an important mechanism for protecting plants from drought, hypothesizes that the distal organs of a plant should be more susceptible to embolism than the basal organs. However, experimental studies testing the VS hypothesis for trees are limited and have reached inconsistent conclusions. Here, we tested the VS hypothesis with three angiosperms and four conifers co-existing in a temperate forest in northeastern China. The results showed that the difference in vulnerability to cavitation between leaves and branches (P50leaf-branch) was positive for the conifers but negative for the angiosperms, implying that the conifers rather than the angiosperms exhibited VS. The conifers had lower leaf hydraulic safety margins and more embolism-resistant branches than the angiosperms. Although the angiosperms did not display VS, they took a hydraulic compensatory strategy (e.g., great leaf and branch hydraulic conductivities) to maintain the water supply of their leaves. In addition, we found a significant trade-off between the sapwood-specific hydraulic conductivity (KSS) and xylem pressure inducing 50% loss of hydraulic conductivity (P50branch) across all species. Both KSS and P50branch increased with the area-based light-saturated photosynthetic rate (Aarea), suggesting that increased embolism resistance of branches comes at the cost of reduced hydraulic efficiency, which in turn constrains the photosynthesis. Aarea was negatively correlated with P50leaf-branch, further indicating that the conifers had strong VS and were associated with a conservative strategy. Conversely, the angiosperms displayed an acquisitive strategy, tending to have higher Aarea, leaf and branch hydraulic conductivities, but lower embolism resistance. These differentiations in the functional traits between the angiosperms and conifers provide potential mechanisms for their co-existence in this temperate forest community.

Leaf traits explaining the growth of tree species planted in a Central Amazonian disturbed area

Tree species selected for planting should exhibit both high survival and fast growth. The growth of a tree or forest plantation is affected by the resource supply (light, water and nutrients), the fraction of resources acquired and resource use efficiency. Leaf traits related to the last two processes have the potential to explain the growth rates. In this study, we evaluated 24 leaf traits (morphological and physiological) at the individual level to investigate whether simple leaf traits can be used to predict the variation in relative growth rates of eight native tree species in a mixed plantation in disturbed areas in Central Amazonia 15 months after planting. Tachigali vulgaris and Trattinnickia rhoifolia exhibited the highest growth rates in both height and diameter, which were approximately three and two times higher than those of Bombacopsis macrocalyx, respectively. Species exhibited different establishment strategies as indicated by the functional leaf trait performance. Tachigali vulgaris, Ochroma pyramidale, Trattinnickia rhoifolia and Ceiba pentandra are efficient resource use species, have high light-saturated photosynthetic rates and are tolerant to high irradiance stress. Endlicheria anomala exhibited the worst performance based on functional traits, with the lowest light-saturated photosynthetic rate (Amax) and maximum quantum yield of photosystem II (FV/FM). Of all traits analyzed, 12 leaf traits were correlated with the relative growth rate (RGR). Leaf traits related to resource acquisition, such as individual leaf area, chlorophyll content, leaf water potential and leaf nutrient concentration, were not good predictors of growth. Only stomatal conductance (gs) was related to the RGR. Leaf traits related to photosynthetic use efficiency (carbon use efficiency and photosynthetic nitrogen and phosphorus use efficiency) explained, on average, 20% and 30% of tree growth in height and diameter, respectively. Resource use efficiency traits were better predictors of growth than the individual physiological traits gs and Amax, which explained, on average, 12% and 19% of the growth in height and diameter, respectively. Photosynthetic efficiency-related traits are good predictors of tree growth, and species with high efficiency – such as T. vulgaris, O. pyramidale, T. rhoifolia and C. pentandra – can achieve high growth in Amazonian disturbed areas. The identification of species with better performance during initial establishment can improve the design of mixture plantations in disturbed areas. Additionally, the selection of traits most correlated with growth performance can be more informative for reforestation monitoring; consequently, previous silvicultural interventions can be adopted prior to the reduction in both growth and survival rates.

Short-term effects of high CO2 accelerate photosynthetic induction in Populus koreana × trichocarpa with always-open stomata regardless of phenotypic changes in high CO2 growth conditions

Long-term high CO2 exposure accelerates photosynthetic induction response due to rapid light increase. However, it is unclear whether the acceleration is caused by acclimation of photosynthetic components (long-term CO2 effect) and/or by the sufficient substrate under high CO2 at the measurement (short-term CO2 effect). Populus koreana × trichocarpa cv. Peace has wide-open stomata almost not responding to changes of photon flux density. Using this species, we examined the long- and short-term CO2 effects on photosynthetic induction by focusing on biochemical components. We grew the plants under [CO2] of 380, 700 and 1020 μmol CO2 mol-1 air and measured the photosynthetic induction response under [CO2] of 380 and 1020 μmol CO2 mol-1 air. Despite significant reduction in Rubisco content and light-saturated photosynthetic rate in the leaves from the high growth CO2, the photosynthetic induction time was similar in leaves from different growth CO2 plants when measurement [CO2] was the same. The induction, however, was significantly fast at the higher than at the lower measurement [CO2], regardless of growth CO2 of the plants. These results demonstrate that the acceleration of apparent photosynthetic induction under high CO2 environment was mainly contributed by a short-term CO2 effect rather than by a long-term acclimation effect when stomatal limitation is not the major factor.

Leaf hydraulic vulnerability triggers the decline in stomatal and mesophyll conductance during drought in rice.

Understanding the physiological responses of crops to drought is important for ensuring sustained crop productivity under climate change, which is expected to exacerbate the frequency and intensity of periods of drought. Drought responses involve multiple traits, and the correlations between these traits are poorly understood. Using a variety of techniques, we estimated the changes in gas exchange, leaf hydraulic conductance, and leaf turgor in rice (Oryza sativa) in response to both short- and long-term soil drought. We performed a photosynthetic limitation analysis to quantify the contributions of each limiting factor to the resultant overall decrease in photosynthesis during drought. Biomass, leaf area, and leaf width significantly decreased during the 2-week drought treatment, but leaf mass per area and leaf vein density increased. Light-saturated photosynthetic rate declined dramatically during soil drought, mainly due to the decrease in stomatal conductance (gs) and mesophyll conductance (gm). Stomatal modeling suggested that the decline in leaf hydraulic conductance explained most of the decrease in stomatal closure during the drought treatment, and may also trigger the drought-related decrease of stomatal conductance and mesophyll conductance. The results of this study provide insight into the regulation of carbon assimilation under drought conditions.

Effects of elevated ozone concentration and nitrogen addition on ammonia stomatal compensation point in a poplar clone

The stomatal compensation point of ammonia (χs) is a key factor controlling plant-atmosphere NH3 exchange, which is dependent on the nitrogen (N) supply and varies among plant species. However, knowledge gaps remain concerning the effects of elevated atmospheric N deposition and ozone (O3) on χs for forest species, resulting in large uncertainties in the parameterizations of NH3 incorporated into atmospheric chemistry and transport models (CTMs). Here, we present leaf-scale measurements of χs for hybrid poplar clone ‘546’ (Populusdeltoides cv. 55/56 x P. deltoides cv. Imperial) growing in two N treatments (N0, no N added; N50, 50 kg N ha−1 yr−1 urea fertilizer added) and two O3 treatments (CF, charcoal-filtered air; E-O3, non-filtered air plus 40 ppb) for 105 days. Our results showed that χs was significantly reduced by E-O3 (41%) and elevated N (19%). The interaction of N and O3 was significant, and N can mitigate the negative effects of O3 on χs. Elevated O3 significantly reduced the light-saturated photosynthetic rate (Asat) and chlorophyll (Chl) content and significantly increased intercellular CO2 concentrations (Ci), but had no significant effect on stomatal conductance (gs). By contrast, elevated N did not significantly affect all measured photosynthetic parameters. Overall, χs was significantly and positively correlated with Asat, gs and Chl, whereas a significant and negative relationship was observed between χs and Ci. Our results suggest that O3-induced changes in Asat, Ci and Chl may affect χs. Our findings provide a scientific basis for optimizing parameterizations of χs in CTMs in response to environmental change factors (i.e., elevated N deposition and/or O3) in the future.

Study on chemical hydrography, chlorophyll-a and primary productivity in Liaodong Bay, China

A field study was carried out during the summer of 2013 in Liaodong Bay, China to determine the dynamics of the phytoplankton in the bay and the extent to which primary production in the bay was constrained by environmental factors. There was little or no evidence of limitation of phytoplankton production by nutrient concentrations at any of the sampling stations, with the possible exception of a few offshore stations where phosphate concentrations were less than 30 nM. This assessment was consistent with the results of nutrient enrichment experiments and the values of light-saturated photosynthetic rates and areal photosynthetic rates. To examine the effects of irradiance and temperature on light-saturated photosynthetic rates normalized to chlorophyll a concentrations (Poptb) at twelve stations where photosynthetic rates were measured by 14C method, light-conditioned values were modeled as a function of the temperature with a satisfactory fit to our field data (R2 = 0.60, p = 0.003). According to this model, the light-conditioned Poptb values increased with temperatures from 22 °C to roughly 25 °C but declined precipitously at higher temperatures, and Poptb values and corresponding areal photosynthetic rates at all 66 stations were estimated to be 7.6 ± 2.4 g C g−1 Chl a h−1 and 532 ± 429 gC m−2 d−1 in average, respectively. The quanta absorbed per carbon atom fixed averaged 14 ± 2 and 37 ± 10 at six coastal stations and six estuarine stations, respectively. The relatively high Poptb values and low quantum requirements at the coastal stations implied the highly efficient usage of absorbed light by phytoplankton under nutrient-replete conditions and favorable temperatures. Comparatively, the low Poptb values and high quantum requirements at the estuarine stations suggested rather extreme light limitation and lowly efficient usage of absorbed light in photosynthesis in the Liaohe River estuary. Areal production in Liaodong Bay appears to be controlled by a combination of temperature and light limitation.

Photosynthetic acclimation of Grammatophyllum speciosum to growth irradiance under natural conditions in Singapore

Grammatophyllum speciosum, a native species to Singapore, have become extinct mainly due to habitat loss. Recently, Singapore has reintroduced G. speciosum into the natural environment under the orchid conservation programme. In this study, leaves of G. speciosum grown under low light (LL) under natural conditions had faster expansion rate and higher specific leaf area than leaves grown under intermediate light (IL) and high light (HL). All leaves had more than 95% midday relative water content. Although midday Fv/Fm ratios were lower in HL leaves than in IL and LL leaves, none of them exhibited chronic photoinhibition. HL leaves had upregulated their light utilization through higher photochemical quantum yield (ΔF/Fm′) and greater electron transport rate. HL leaves also had higher non-photochemical quenching, indicating that they had higher capability to dissipate excess light as heat, which was supported by their lower chlorophyll but higher carotenoids content. Although there was a linear correction between leaf temperature and photosynthetic photon flux density (PPFD), no correlations were found between stomatal conductance (gs) and PPFD, gs and leaf temperature. Light-saturated photosynthetic CO2 assimilation rate (Asat) was significantly higher in HL leaves than those of IL and LL leaves. However, all leaves had similar light-saturated stomatal conductance. Although LL leaves had higher leaf total reduced nitrogen that those of IL and HL leaves, none of them seemed to suffer from nitrogen deficiency during the experimental period. To conclude, G. speciosum is able to survive under different growth irradiances without watering and adding fertilizers.

Maize leaf functional responses to drought episode and rewatering

Effects of crop growth and physiological activities to drought and irrigation regimes have been extensively studied; however, the combined responses of plant growth, morphological and photosynthetic behaviors to drought episodes and thereafter rewatering receive a less attention. This field experiment was carried out directly in situ at an agricultural ecosystem research station across two entire growing seasons during 2015–2016, in northeastern China, on the renowned northeastern maize production belt, where is being threatened by severe drought. A field automatic rain-shelter was used, and five irrigation regimes including control, four drought episodes, and rewatering treatments were established. The chlorophyll contents (SPAD values), light-saturated photosynthetic rate (Asat), and photosystem II actual quantum yield (ΦPSII), maximum quantum yield (Fv′/Fm′) decreased at lower leaf positions and with plant development. Episodic drought effects on plant growth, leaf morphological traits and photosynthetic processes at both vegetative and reproductive stages were severely remarked, particularly at late development stage and with longer drought duration. The recovery of leaf functional traits of the plants experienced historical-drought following re-irrigating was not fully restored to the level of the plants subjected to ample and normal water status; and the strength of recovery was proportional to the persistence of pre-drought episodes. The relationship of Asat with SPAD depends on water status and plant development. A principal component analysis can well denote the change patterns in responses to water status treatments with plant development. The results may give an insight into how to understand the maize traits’ responses to drought episode and rewatering, and this also might assist the drought-stricken crops to cope with future climatic change.

Anatomical and diffusional determinants inside leaves explain the difference in photosynthetic capacity between Cypripedium and Paphiopedilum, Orchidaceae.

Comparing with other angiosperms, most members within the family Orchidaceae have lower photosynthetic capacities. However, the underlying mechanisms remain unclear. Cypripedium and Paphiopedilum are closely related phylogenetically in Orchidaceae, but their photosynthetic performances are different. We explored the roles of internal anatomy and diffusional conductance in determining photosynthesis in three Cypripedium and three Paphiopedilum species, and quantitatively analyzed their diffusional and biochemical limitations to photosynthesis. Paphiopedilum species showed lower light-saturated photosynthetic rate (A N), stomatal conductance (g s), and mesophyll conductance (g m) than Cypripedium species. A N was positively correlated with g s and g m. And yet, in both species A N was more strongly limited by g m than by biochemical factors or g s. The greater g s of Cypripedium was mainly affected by larger stomatal apparatus area and smaller pore depth, while the less g m of Paphiopedilum was determined by the reduced surface area of mesophyll cells and chloroplasts exposed to intercellular airspace per unit of leaf area, and much thicker cell wall thickness. These results suggest that leaf anatomical structure is the key factor affecting g m, which is largely responsible for the difference in photosynthetic capacity between those two genera. Our findings provide new insight into the photosynthetic physiology and functional diversification of orchids.

Arbuscular mycorrhizal fungi alter nitrogen allocation in the leaves of Populus × canadensis ‘Neva’

Intra-leaf nitrogen allocation plays a pivotal role in plant growth performance; however, the effects of arbuscular mycorrhizal (AM) fungi on the allocation of nitrogen within a leaf remain poorly understood. A pot experiment was conducted with different nitrogen levels and Populus × canadensis ‘Neva’ with or without Rhizophagus irregularis inoculation. The fractions of leaf nitrogen allocated to water-soluble protein, membrane-bound protein, cell wall protein and photosynthetic apparatus were used to estimate nitrogen allocation strategy. AM fungi increased the light-saturated photosynthetic rate (Pmax) and photosynthetic nitrogen use efficiency (PNUE) under low nitrogen levels, whereas the nitrogen content per unit area (Narea) and nitrogen content per unit mass (Nmass) were unaffected. AM inoculation decreased leaf mass per area (LMA) and the fraction of leaf nitrogen allocated to cell walls but increased the fraction of nitrogen allocated to photosynthesis under low nitrogen levels. The increased fraction of photosynthetic nitrogen allocated to carboxylation (PC) and bioenergetics (PB) in response to AM inoculation under low nitrogen levels contributed to the higher Pmax and PNUE values. The improved photosynthesis and PNUE resulting from AM inoculation were mostly determined by the relative allocation of nitrogen to photosynthesis and not by the leaf nitrogen concentration. AM fungi may enhance nitrogen allocation to photosynthesis at the expense of cell walls.

No significant interactions between nitrogen stimulation and ozone inhibition of isoprene emission in Cathay poplar

Isoprene emission from plants subject to a combination of ozone (O3) and nitrogen (N) has never been investigated. Cathay poplar (Populus cathayana) saplings were exposed to O3 (CF, charcoal-filtered air, NF, non-filtered ambient air and E-O3, non-filtered air +40ppb) and N treatments (N0, 0kgNha−1year−1, N50, 50kgNha−1year−1 and N100, 100kgNha−1year−1) for 96days. Increasing O3 exposure decreased isoprene emission (11.5% in NF and 57.9% in E-O3), as well as light-saturated photosynthetic rate (Asat) and chlorophyll content, while N load increased isoprene emission (19.6% in N50 and 33.4% in N100) as well as Asat and chlorophyll content. Although O3 and N interacted significantly in Asat, N did not mitigate the negative effects of O3 on isoprene emission, i.e. the combined effects were additive and did not interact. These results warrant more research on the combined effects of co-existing global change factors on future isoprene emission and atmospheric chemical processes.

Higher photosynthesis, nutrient- and energy-use efficiencies contribute to invasiveness of exotic plants in a nutrient poor habitat in northeast China.

The roles of photosynthesis-related traits in invasiveness of introduced plant species are still not well elucidated, especially in nutrient-poor habitats. In addition, little effort has been made to determine the physiological causes and consequences of the difference in these traits between invasive and native plants. To address these problems, we compared the differences in 16 leaf functional traits related to light-saturated photosynthetic rate (Pmax ) between 22 invasive and native plants in a nutrient-poor habitat in northeast China. The invasive plants had significantly higher Pmax , photosynthetic nitrogen- (PNUE), phosphorus- (PPUE), potassium- (PKUE) and energy-use efficiencies (PEUE) than the co-occurring natives, while leaf nutrient concentrations, construction cost (CC) and specific leaf area were not significantly different between the invasive and native plants. The higher PNUE contributed to higher Pmax for the invasive plants, which in turn contributed to higher PPUE, PKUE and PEUE. CC changed independently with other traits such as Pmax , PNUE, PPUE, PKUE and PEUE, showing two trait dimensions, which may facilitate acclimation to multifarious niche dimensions. Our results indicate that the invasive plants have a superior resource-use strategy, i.e. higher photosynthesis under similar resource investments, contributing to invasion success in the barren habitat.

Differential responses of peach (Prunus persica) seedlings to elevated ozone are related with leaf mass per area, antioxidant enzymes activity rather than stomatal conductance

To evaluate the ozone (O3) sensitivity among peach tree (Prunus persica) cultivars widely planted in Beijing region and explore the possible eco-physiological response mechanisms, thirteen cultivars of peach seedlings were exposed to either charcoal-filtered air or elevated O3 (E-O3, non-filtered ambient air plus 60 ppb) for one growing season in open-top chambers. Leaf structure, stomatal structure, gas exchange and chlorophyll a fluorescence, photosynthetic pigments, antioxidant defense system and lipid peroxidation were measured in three replicated chambers. Results showed that E-O3 significantly reduced abaxial epidemis thickness, but no effects on the thicknesses of adaxial epidemis, palisade parenchyma and spongy parenchyma. Stomatal area, density and conductance were not significantly affected by E-O3. E-O3 significantly accelerated leaf senescence, as indicated by increased lipid peroxidation and more declines in light-saturated photosynthetic rate and pigments contents. The reduced ascorbate content (ASC) was decreased but antioxidant enzyme activity (CAT, APX and SOD) and total antioxidant capacity (TAC) were significantly increased by E-O3 among cultivars. The cultivars with visible symptoms also had more reductions in net photosynthetic rate than those without visible symptoms. Ozone sensitivity among cultivars was strongly linked to leaf mass per area (LMA), antioxidant enzymes activity e.g. SOD, APX rather than stomatal parameters (stomatal area, density and conductance) and ASC. Results could provide a theoretical basis for selecting and breeding the ozone-resistant cultivars of peach trees grown in high O3-polluted regions.

Spring phytoplankton communities of the Labrador Sea (2005–2014): pigment signatures, photophysiology and elemental ratios

Abstract. The Labrador Sea is an ideal region to study the biogeographical, physiological, and biogeochemical implications of phytoplankton community composition due to sharp transitions between distinct water masses across its shelves and central basin. We have investigated the multi-year (2005–2014) distributions of late spring and early summer (May to June) phytoplankton communities in the various hydrographic settings of the Labrador Sea. Our analysis is based on pigment markers (using CHEMTAX analysis), and photophysiological and biogeochemical characteristics associated with each phytoplankton community. Diatoms were the most abundant group, blooming first in shallow mixed layers of haline-stratified Arctic shelf waters. Along with diatoms, chlorophytes co-dominated at the western end of the section (particularly in the polar waters of the Labrador Current (LC)), whilst Phaeocystis co-dominated in the east (modified polar waters of the West Greenland Current (WGC)). Pre-bloom conditions occurred in deeper mixed layers of the central Labrador Sea in May, where a mixed assemblage of flagellates (dinoflagellates, prasinophytes, prymnesiophytes, particularly coccolithophores, and chrysophytes/pelagophytes) occurred in low-chlorophyll areas, succeeding to blooms of diatoms and dinoflagellates in thermally stratified Atlantic waters in June. Light-saturated photosynthetic rates and saturation irradiance levels were highest at stations where diatoms were the dominant phytoplankton group ( > 70 % of total chlorophyll a), as opposed to stations where flagellates were more abundant (from 40 up to 70 % of total chlorophyll a). Phytoplankton communities from the WGC (Phaeocystis and diatoms) had lower light-limited photosynthetic rates, with little evidence of photoinhibition, indicating greater tolerance to a high light environment. By contrast, communities from the central Labrador Sea (dinoflagellates and diatoms), which bloomed later in the season (June), appeared to be more sensitive to high light levels. Ratios of accessory pigments (AP) to total chlorophyll a (TChl a) varied according to phytoplankton community composition, with polar phytoplankton (cold-water related) having lower AP : TChl a. Polar waters (LC and WGC) also had higher and more variable particulate organic carbon (POC) to particulate organic nitrogen (PON) ratios, suggesting the influence of detritus from freshwater input, derived from riverine, glacial, and/or sea ice meltwater. Long-term observational shifts in phytoplankton communities were not assessed in this study due to the short temporal frame (May to June) of the data. Nevertheless, these results add to our current understanding of phytoplankton group distribution, as well as an evaluation of the biogeochemical role (in terms of C : N ratios) of spring phytoplankton communities in the Labrador Sea, which will assist our understanding of potential long-term responses of phytoplankton communities in high-latitude oceans to a changing climate.

Differences in the photosynthetic plasticity of ferns and Ginkgo grown in experimentally controlled low [O2]:[CO2] atmospheres may explain their contrasting ecological fate across the Triassic-Jurassic mass extinction boundary.

Background and Aims Fluctuations in [CO2] have been widely studied as a potential driver of plant evolution; however, the role of a fluctuating [O2]:[CO2] ratio is often overlooked. The present study aimed to investigate the inherent physiological plasticity of early diverging, extant species following acclimation to an atmosphere similar to that across the Triassic–Jurassic mass extinction interval (TJB, approx. 200 Mya), a time of major ecological change. Methods Mature plants from two angiosperm (Drimys winteri and Chloranthus oldhamii), two monilophyte (Osmunda claytoniana and Cyathea australis) and one gymnosperm (Ginkgo biloba) species were grown for 2 months in replicated walk-in Conviron BDW40 chambers running at TJB treatment conditions of 16 % [O2]–1900 ppm [CO2] and ambient conditions of 21 % [O2]–400 ppm [CO2], and their physiological plasticity was assessed using gas exchange and chlorophyll fluorescence methods. Key Results TJB acclimation caused significant reductions in the maximum rate of carboxylation (VCmax) and the maximum electron flow supporting ribulose-1,5-bisphosphate regeneration (Jmax) in all species, yet this downregulation had little effect on their light-saturated photosynthetic rate (Asat). Ginkgo was found to photorespire heavily under ambient conditions, while growth in low [O2]:[CO2] resulted in increased heat dissipation per reaction centre (DIo/RC), severe photodamage, as revealed by the species’ decreased maximum efficiency of primary photochemistry (Fv/Fm) and decreased in situ photosynthetic electron flow (Jsitu). Conclusions It is argued that the observed photodamage reflects the inability of Ginkgo to divert excess photosynthetic electron flow to sinks other than the downregulated C3 and the diminished C2 cycles under low [O2]:[CO2]. This finding, coupled with the remarkable physiological plasticity of the ferns, provides insights into the underlying mechanism of Ginkgoales’ near extinction and ferns’ proliferation as atmospheric [CO2] increased to maximum levels across the TJB.

Effects of deer on the photosynthetic performance of invasive and native forest herbs.

Overabundant generalist herbivores can facilitate non-native plant invasions, presumably through direct and indirect modifications to the environment that affect plant performance. However, ecophysiological mechanisms behind ungulate-mediated plant invasions have not been well-studied. At a long-term Odocoileus virginianus (white-tailed deer) exclusion site in a temperate deciduous forest, we quantified deer-mediated ecophysiological impacts on an invasive biennial Alliaria petiolata (garlic mustard) and two palatable native herbaceous perennials, Maianthemum racemosum and Trillium grandiflorum. In mid-summer, we found that leaf-level light availability was higher in unfenced areas compared with areas fenced to exclude deer. Alliaria in unfenced areas exhibited 50 % higher mean maximum photosynthetic rates compared with fenced areas. Further, specific leaf area decreased by 48 % on average in unfenced areas, suggesting leaf structural responses to higher light levels. Similarly, Maianthemum had 42 % higher mean photosynthetic rates and 33 % decreased mean specific leaf area in unfenced areas, but these functional advantages were likely countered by high rates of deer herbivory. By contrast, Trillium exhibited significantly lower (26 %) maximum photosynthetic rates in unfenced areas, but SLA did not differ. Deer-mediated differences in light saturated photosynthetic rates for all three species were only significant during months with overstory tree canopy cover, when light availability in the herb layer was significantly lower in fenced areas. Alliaria's enhanced photosynthetic rates implicate overabundant deer, a situation that is nearly ubiquitous across its invaded range. Collectively, our results provide empirical evidence that generalist herbivores can alter non-native plant physiology to facilitate invasion.

Amelioration of drought-induced negative responses by elevated CO2 in field grown short rotation coppice mulberry (Morus spp.), a potential bio-energy tree crop.

Present study describes the responses of short rotation coppice (SRC) mulberry, a potential bio-energy tree, grown under interactive environment of elevated CO2 (E) and water stress (WS). Growth in E stimulated photosynthetic performance in well-watered (WW) as well as during WS with significant increases in light-saturated photosynthetic rates (A Sat), water use efficiency (WUEi), intercellular [CO2], and photosystem-II efficiency (F V/F M and ∆F/F M') with concomitant reduction in stomatal conductance (g s) and transpiration (E) compared to ambient CO2 (A) grown plants. Reduced levels of proline, H2O2, and malondialdehyde (MDA) and higher contents of antioxidants including ascorbic acid and total phenolics in WW and WS in E plants clearly demonstrated lesser oxidative damage. Further, A plants showed higher transcript abundance and antioxidant enzyme activities under WW as well as during initial stages of WS (15 days). However, with increasing drought imposition (30 days), A plants showed down regulation of antioxidant systems compared to their respective E plants. These results clearly demonstrated that future increased atmospheric CO2 enhances the photosynthetic potential and also mitigate the drought-induced oxidative stress in SRC mulberry. In conclusion, mulberry is a potential bio-energy tree crop which is best suitable for short rotation coppice forestry-based mitigation of increased [CO2] levels even under intermittent drought conditions, projected to prevail in the fast-changing global climate.

Photosynthetic response of four fern species from different habitats to drought stress: relationship between morpho-anatomical and physiological traits

Ferns flourish in many habitats, from epiphytic to terrestrial and from sunny to shady, and such varied conditions require contrasting photosynthetic strategies to cope with drought. Four species of temperate ferns from different habitats were subjected to drought by withholding irrigation in order to investigate their photosynthetic responses. Lepisorus thunbergianus (epiphytic) had low stomatal density and showed high water-use efficiency (WUE) retaining photosynthetic activity with low relative frond water content under drought stress, which suggested their high adaptation to drought. On the other hand, low WUE with low light-saturated photosynthetic rate in Adiantum pedatum (terrestrial, shady environment) was associated with much lower photosynthesis than in the other species under drought stress, suggesting lower adaptation to drought-prone habitats. Morphological stomatal traits such as stomatal density and photosynthetic response to drought in ferns involved species-specific adaptation to survive and grow in their natural habitats with different levels of drought.