Fish-collection and transportation systems (FCTS) have been introduced and applied as innovative approaches to facilitate fish passage in China for several years. The efficacy of FCTSs depends directly on the efficiency of the fish-guidance measures employed. In this study, the effects of three parameters—water flow, artificial lighting, and bubble curtains—on the attraction of mosquitofish (Gambusia affinis) was investigated using a fish barge model in a large pond. The experiment was conducted in two steps. First, a univariate experiment was performed, and then, a multivariate experiment using an orthogonal design was performed. Four different colors of light were tested: 1) yellow light (570 nm), 2) blue light (460 nm), 3) warm white light (3000 K), and 4) a weak light control group, whose illuminance belongs to the interval 0.001 lx to 0.3 lx. Supplementing water flow within the barge decreased the number of attracted fish (i.e., the number of the targeted fish species that appeared within 1.5 m of the barge model’s entrance) but increased the fish-collection ratio of the fish-collecting platform (i.e., the ratio of the number of attracted fish to the number of collected fish). Warm white light was shown to be significantly effective in attracting fish, while no obvious effect was observed in terms of the fish-collection ratio. The bubble curtain exhibited excellent fish-attraction efficacies, but also had no significant effect on the attraction and collection efficacy of the fish-collecting platform. During the day, the optimal combination for fish attraction was to have no water flow with a bubble curtain, while the optimal combination for fish collection was to have no water flow and no bubble curtain. During the night, the optimal combination for fish attraction was to have no water flow, no bubble curtain, and a warm white light, while the optimal combination for fish collection was to have water flow, no bubble curtain, and a weak light. This study tested the combined efficacies of various fish-guidance measures on a fish-collecting platform, thus providing experimental support for optimizing various fish-attraction measures and the application of these measures in FCTS projects.
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