Mass timber composites (MTCs), where the webs are typically spaced 750–2000 mm apart, have shown potential in overcoming span limitations of conventional flat slab mass timber floors. However, fundamental research investigating the non-linear stress distribution (i.e., shear lag) in the flanges is required. While this behaviour has been simplified in design for other materials using the concept of an effective flange width (EFW), no guidance exists in international design codes for mass timber composites. In the current study, a parametric analysis was conducted on a mass timber I-beam under positive bending where the effects of web spacing, beam configuration (edge or interior placement), shear connection stiffness, concentrated versus uniform loading, web depth, web width, flange thickness, and flange modulus of elasticity on the EFW, were investigated. The reference section in the finite element model consisted of a glued-laminated timber (GLT) panel for the flanges, a glulam beam web, and a fully composite glued connection between elements.The results have shown that existing design code provisions, even those meant for light-frame wood members, over-estimate the EFW of GLT-glulam I-beams. The web spacing, shear connection stiffness, loading type, flange thickness, and web width were observed to be the most influential parameters on the EFW of GLT-glulam MTCs. Between the upper and lower boundaries of connection stiffness, a sharp increase in the EFW was observed below the point of full composite action due to the section beginning to behave as three separate elements. Although, the decrease in composite action resulted in an overall decrease in the bending stiffness of the section. For GLT-glulam MTCs, the modulus of elasticity (MOE) of the GLT flange was not observed to significantly affect the EFW for high degree of composite action configurations. A small increase in the EFW was observed when the degree of composite action was relatively low. Ultimately, the development of equations for quantifying the EFW of MTCs is key to their increased adoption but requires the expansion of the current study to include other mass timber elements, as well as experimental data investigating the most significant parameters identified herein.