Light is an important component in poultry production, and it may impact bird behavior, an important component of animal welfare. Light-emitting diode (LED) lamps are of interest for broiler production since they are inexpensive to run and provide monochromatic colors. This study aimed to understand the impact of three light colors (blue, green, or white), provided by LED lighting, on behavioral expression, stress and fear levels of broilers. A total of 14,256 male and female broilers of 2 genotypes (Ross EPMx708 and Ross YPMx708) were housed in 9 rooms in 2 blocked trials (3 room replicates per light per trial), with sexes and genotypes housed in 12 separate pens per room. Behavioral expression was recorded using an infrared camera and analyzed using a scan sampling technique. To assess fear, 3 tests were conducted: tonic immobility, novel object, and response to observer. Blood was collected to evaluate chronic stress using the heterophil:lymphocyte (H:L) ratio. Data were statistically analyzed using SAS (MIXED procedure) in a 3 × 2 × 2 factorial design, with lighting treatment nested within room. Fear tests indicated reduced fear levels in birds raised under blue light (lower latency to rise during the tonic immobility test and a lower percentage of birds moving due to the passage by of an observer). No differences were observed for the novel object test. Light color resulted in changes in stress levels, indicated by a lower H:L ratio for broilers raised under blue light compared to those raised under white light. Behavior was influenced by light color, especially at 33 to 34 d of age, where birds raised under white light were more active, and birds raised under blue light spent more time resting. Overall, results indicated that light color has minor influences on behavioral expression. Utilizing blue light during the brooding and rearing phase leads to lower stress and a reduction in fear, suggesting that blue light may improve the emotional states of fear and stress, thereby improving the welfare of poultry.
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