Controlling food intake by suppressing appetite can potentially be one of the principal approaches to preventing weight gain and obesity. A major gut hormone involved in appetite control is cholecystokinin (CCK), where previous experiments showed that polyunsaturated fatty acids (PUFAs) derived from Korean pine nuts (Pinus koraiensis) PinnoThin™ induces high amounts of CCK release by STC-1 enteroendocrine cells. This study investigates the effects of PinnoThin™ on appetite sensations and appetite-regulating hormones in humans. A randomized, cross-over, placebo-controlled, double-blind study was performed with 18 overweight post-menopausal women (BMI=25–30 kg/m 2 ) receiving capsules with 3 g PinnoThin™ or olive oil (placebo) with a light breakfast. At 0, 30, 60, 90, 120, 180 and 240 min following supplementation blood samples were taken for analyses of appetite suppressing hormones CCK and glucagon-like peptide-1 (GLP-1). Appetite sensations were evaluated by using visual analogue scales. PinnoThin™ significantly induced CCK after 30 min and GLP-1 after 60 min relative to placebo. Over a period of 4 h the total amount of plasma CCK and GLP-1 in response to PinnoThin™ was 60% ( P P