Photosynthetic activity of intact nursery-grown white spruce (Piceaglauca (Moench) Voss) seedlings was assessed by measuring CO2 exchange (apparent photosynthesis) and normalized, integrated variable chlorophyll fluorescence emission. Agreement between fluorescence and apparent photosynthesis indicated that photosynthetic inactivation of seedlings from selected seedlots began in mid-August and approached completion in late October. Inactivation occurred somewhat earlier in northern seedlot seedlings than in those from a more southerly provenance. Seedlings tested in late October showed significant photosynthetic inactivation, as indicated by both fluorescence and apparent photosynthesis. These seedlings also had passed the −18 °C frost hardiness test currently used in British Columbia as an indicator for safe lifting. On removal from −2 °C storage, seedlings lifted and stored according to nursery practises showed fluorescence emission indicative of photosynthetic reactivation and also had high root growth capacity scores. Low root growth capacity scores were associated with delayed or incomplete photosynthetic reactivation. These results show that fluorescence assessment provides information about the physiological status of white spruce seedlings. Variable fluorescence assays are nondestructive and can be made and interpreted within minutes. As an indicator of shoot metabolic activity, fluorescence assessment provides information useful in selecting lifting dates and in evaluating the effects of dark cold storage on white spruce seedlings.